• Title/Summary/Keyword: Mixed tea

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The Cytotoxicity and Chemosensitizing Effects of native camellia(Camellia japonica) and nutraceutical camellia teas

  • Hwang, Eun-Joo;Park, Min-Hee;Pyo, Byoung-Sik;Cha, Young-Ju;Lee, Sook-Young
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2003.04a
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    • pp.102-102
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    • 2003
  • The present study has been undertaken to characterize availability of camellia(Camellia japonica L.) as a medicinal plant with antineoplastic and chemosensitizing activities. The crude extracts from fresn camellia flower, young leaves and nutraceutical tea of camellia leaf and flower buds were evaluated on their potential activities against various human cancer cells and multidrug resistance to cancer cells in vitro. The range of cytotoxicity displayed from 120$\mu\textrm{g}$/mL to 200$\mu\textrm{g}$/mL. Catemix 1(CT-1) mixed with camellia and green tea showed high toxicity(respectively IC$\sub$50/=l16$\mu\textrm{g}$/mL, 129$\mu\textrm{g}$/mL) against AML-2/WT, acute myelogenous leukemia cell and MCF-7, brest adenocarcinoma pleual effusion cell. Generally camellia tea mixed with green tea showed higher cytotoxicity than the other camellia teas mixed with some herbs(CH). Methanol extract of steamed camellia tea and roasted camellia tea had a chemosensitizing effect to reverse Pgp-mediated MDR. In addition, camellia flower tea of insignificant cytotoxicity, chemosensitizing effect were increased remarkably chemosensitizing effect in mixed flower tea with some herbs.

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Sensuous Characteristics and Physiological Activity of Cheongtaejeon Tea Produced with the Inoculation of Microbial Strains (균주를 접종하여 제조한 청태전 차의 관능적 특성과 생리활성 효과)

  • Heo, Buk-Gu;Cho, Jung-Il;Park, Yong-Seo;Park, Yun-Jum;Cho, Ja-Yong
    • The Korean Journal of Community Living Science
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    • v.21 no.1
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    • pp.139-148
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    • 2010
  • This study was conducted to restore our traditional Cheongtaejeon tea and to develop the special products. We inoculated Cheongtaejeon tea with lactobacilli (Lactobacillus plantarum CHO 25) and the mixed microbial strains (L. plantarum CHO 25 + Saccharomyces cerevisiae + Bacillus amyloliquefaciens CHO 104). We also examined the sensuous characteristics and physiological activity of Cheongtaejeon tea which was produced by the inoculation of microbial strains. The external appearance of Cheongtaejeon teas were not significant among the teas which were produced with or without the inoculation of L. plantarum CHO 25 and the mixed microbial strains. The taste of the tea increased most in Cheongtaejeon tea which was produced without the inoculation of microbial strains. The taste and liking of Cheongtaejeon tea which was inoculated with Aspergillus oryzae and Aspergillus niger decreased significantly, and it was not suitable to drink. Total phenolics compound contents, total flavonoid contents and DPPH ($\alpha,\alpha$-diphenyl-$\beta$-picryl-hydrazyl) radical scavenging activity of Cheongtaejeon tea extracts increased much more in the order of that produced with the inoculation of L. plantarum CHO 25, control and that with the mixed microbial strains. However, nitrite radical scavenging activity in 1,000 mg/L Cheongtaejeon tea hot water extracts were in the order of the control (94.4%), the inoculation of L. plantarum CHO 25 (93.6%) and the mixed microbial strains (91.1%). Overall results indicated that the sensuous characteristics increased most in Cheongtaejeon tea which was produced without the inoculation of microbial strains, and those physiological activities in tea with the inoculation of L. plantarum CHO 25.

Characterization of Kombucha Beverages Fermented with Various Teas and Tea Fungus

  • Lee, Sam-Pin;Kim, Chan-Shick
    • Preventive Nutrition and Food Science
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    • v.5 no.3
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    • pp.165-169
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    • 2000
  • Kombucha beverages were made from sweetened tea by Oriental, European and Tibetan tea fungus starters. The hot water extracts o green tea, black tea, Gugija and Omija were mixed with white and/or brown sugar, and were fermented under a static culture at 3$0^{\circ}C$. Titrable acidity, pH, color and cellulose production in kombucha beverages were evaluated. All tea fungus starters showed a higher acid production in green/black tea extracts rather than Gugija and Omija extracts. In green/black tea extracts Oriental tea fungus produced a kombucha beverage with a higher titrable acidity and lower pH than those of European and Tibetan tea fungus starters. By the static fermentation of green/black tea extract for 18 days, Oriental, Tibetan and European tea fungus starters produced cellulose pellicles of 0.43g, 0.16g, and 0.19 g (dry weight) on the top in the culture, respectively. As a mother starter, the cellulose pellicle was more efficient in acid production compared with tea fungus broth. Oriental/Tibetan mixed tea fungus showed the best acid production in the green/black tea extract supplemented with brown sugar.

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Isolating Microorganisms to Ferment Traditional Cheongtaejeon (발효차 청태전 제조용 미생물의 분리)

  • Park, Jung-Suk;Cho, Jung-Il
    • Journal of the Korean Society of Food Culture
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    • v.26 no.2
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    • pp.190-197
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    • 2011
  • Chungtaejeon is a traditional tea introduced in the age of the Three States and is the only "Don-cha" culture in the world that survived on the southwestern shore of Korea. To restore Chungtaejeon and to make the tea with consistent quality, the microorganisms involved in traditional type fermentation of Chungtaejeon were isolated, and the tea was prepared with high fermentation ability starters. The sensuous characteristics of Chungtaejeon were also examined. Only Bacilli were found in 3 and 5 year aged Chungtaejeon samples. The Lactobacilli were isolated from properly fermented kimchi and one of them showed high growth capability in media containing green tea extract and also showed strong antagonistic activity against methicillin-resistant Staphylococcus aureus, S. aureus, Salmonella, and E. coli. It was identified and named Lactobacillus plantarum CHO25. Chungtaejeon was fermented with a single starter of L. plantarum CHO25 and with a mixed starter (L. plantarum CHO25, Saccharomyces cerevisiae and Bacillus amyloliquefaciens CHO104). The single fermented sample had the highest cell growth after 5 days of inoculation and the level decreased slowly thereafter. The mixed fermented sample showed strong growth of S. cerevisiae. The highest hunter values were the a value of the single fermented sample and the b value of the mixed sample. The single fermented tea showed the best incense score.

Determination of hesperidin in mixed tea by HPLC (HPLC를 이용한 혼합차의 Hesperidin 정량)

  • Han, Saem;Mok, So-Youn;Kim, Hye-Min;Lee, Jeong-Min;Lee, Dong-Gu;Lee, Sook-Young;Kim, Jong-Kee;Kim, Sun-Ju;Lee, Sang-Hyun
    • Korean Journal of Agricultural Science
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    • v.38 no.2
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    • pp.295-299
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    • 2011
  • The content of hesperidin in the mixed tea, which was composed of dried orange peel, laurel leaf, mulberry leaf, silver magnolia leaf, oriental melon tap, cassia seed, and licorice root, was determined by high performance liquid chromatography (HPLC). Hesperidin was quantified by a reverse phase column with gradient solvent system (watcr:acetonitrile = 80:20 to 35:65 for 30 min) and UV/VIS detection (280 nm). The How rate was kept constant at 1.0 ml/min. The content of hesperidin in the mixed tea was measured in depending on extraction time 1, 2, 3, and 4 min (29.07, 52.39, 52.45, and 88.35 mg/g, respectively).

Evaluation of Mixed Treatment of Amino Acid Liquid Fertilizer and Compost Tea as a Substitute for Oil-cake in Organic Cultivation of Maize (옥수수 유기재배에서 유박 대체제로써 아미노산액비와 퇴비차의 혼합처리 평가)

  • Lee, Sung-Hee;Lee, Kyu-Hoi;Kim, Hyun-Ju;Lee, Sang Min;Kim, Ju-Hyoung
    • Korean Journal of Organic Agriculture
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    • v.27 no.4
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    • pp.453-461
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    • 2019
  • The purpose of this study was to evaluate the mixed treatment of compost tea and amino acid liquid fertilizer as a substitute of oil cake for the organic cultivation of maize in Goesan, Chungbuk province. As a result, nitrogen, phosphoric acid, potassium, calcium and magnesium contents of each organic fertilizer were in the order of oil cake > amino acid fertilizer > compost tea. Each of these organic fertilizers had little influence on the change of soil chemical. Individual treatment of amino acid fertilizer and oil cake for weight of an ear as well as all individual treatments for the fresh weight of 100 kernels showed significant increase, compared to the untreated control. For the corn yield of merchantable quality, each treatment of compost tea, amino acid liquid fertilizer and oil cake increased higher 3.9, 5.4 and 5.9% than untreated control, respectively. On the other hand, as a result of mixed treatment of amino acid fertilizer and compost tea, the change of soil chemical property was insignificant. The mixed treatment, Nonetheless, showed significant increase for stem height to 1st ear, grain setting length in an ear, weight of an ear, and fresh weight of 100 kernels, compared to the oil cake as a control. After all, the corn yield of merchantable quality produced by the mixed treatment was 3.9% higher than the control. Therefore, our study would be considered that the mixed treatment of amino acid and compost tea is one of organic materials that can replace oil-cake in maize.

Dental erosive effects of fluoride-containing tea beverages with low pH (불소를 함유한 pH가 낮은 액상차의 치아 부식 위험도 평가)

  • Seon, Su-Yun;Yun, In-Gyeong;Kim, Ji-Eun;Jeong, Seong-Soog;Choi, Choong-Ho
    • Journal of Korean Academy of Oral Health
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    • v.42 no.4
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    • pp.118-123
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    • 2018
  • Objective: This study aimed to investigate the effects of fluoride-containing acidic tea beverages on bovine teeth surfaces. Methods: The pH values and fluoride concentrations of 11 kinds of mineral water and 23 kinds of tea were measured. The fluoride-containing Seopyenje black tea beverages with pH 3.48 were chosen for the experimental group. Distilled water that did not contain fluoride and had the same pH value as that of the Seopyenje black teas served as the positive control. The Jeju Samdasoo mineral waters with neural pH (pH 7.52) and no fluoride were chosen as the negative control. Bovine teeth in each group were submerged for 10 minutes. Thereafter, surface microhardness of each bovine teeth was measured and the results were analyzed. Results: The pH value range was 6.94-8.09 ($mean=7.37{\pm}0.08$) for the 11 kinds of mineral water and 3.48-6.74 ($5.62{\pm}0.05$) for the 23 tea beverages. Titratable acidity of the Seopyenje black tea beverages was higher than that of the distilled water mixed with citric acid, and the pH was 5.5 and 7.0, respectively. The fluoride content of the 11 kinds of mineral water was 0-1.09 ppm ($mean=0.44{\pm}0.02ppm$) that of the 23 tea beverages was and 0-0.74 ppm ($0.64{\pm}0.06ppm$). In terms of enamel microhardness reduction of the bovine teeth, the tea beverages had the largest effects. There was no significant difference in microhardness reduction between the tea beverages and distilled water mixed with citric acid (P>0.05). Conversely, a significant difference was found between the kinds of mineral water and tea beverages, and also between mineral water and distilled water mixed with citric acid (P<0.05). Conclusions: Acidic tea beverages appeared to erode the surfaces of bovine teeth. The amount of fluoride in the tea beverages was not enough to inhibit erosion. Therefore, frequent intakes of acidic tea beverage are likely to increase erosion on the surfaces of bovine teeth, by affecting the enamel microhardness of teeth.

Effect of Green Tea on Mixed Functon Oxidase System and Xanthine Oxidase Activities in Rat Liver Exposed to Microwaves (전자파를 조사한 흰쥐 간조직에서의 Mixed Function Oxidase System과 Xanthine Oxidase 활성에 미치는 녹차의 영향)

  • 이순재;이용희
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.29 no.1
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    • pp.168-173
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    • 2000
  • The purpose of this study was to investigate the changes of microsomal mixed function oxidase (MFO) and xanthine oxidase activities (XOD) in the liver of rats exposed to microwave. Sprague-Dawley male rats weighing 200$\pm$10g body weight were randomly assigned to a normal and microwave exposed(MW) groups; microwave exposed groups were divided into two groups; microwave (MW) group and green tea(GT) gropu which were fed distilled water and green tea extracts during experimental periods, respectively. Rats were irradiated with microwave at the frequency of 2.45 GHz for 15min and rats wre sacrificed at the 4th day of the microwave irradiaton. The hemoglobin level of GT group was higher than that of the normal gropu and MW group, but the hematocrit value was not significantly different among all experimental gropus. The activity of serum GOT of MW group was significantly increased but that of GT group was similar to normal group. Activities of GPT were not significantly different among all experimental groups. Liver XOD activity was significantly increased in the microwave exposed groups but green tea normalized the XOD activity. The activity of hepatic microsomal cytochrome P450 was significantly increased in MW group compared to normal group and that of GT group was similar to that of the normal group. The activity of hepatic microsomal NADPH-cytochrome P450 reductase was also significantly increased in MW group compared to normal group, but that of GT group was similar to that fo the normal group. In conclusion, the activities of MFO and XOD were elevated by microwave irradiaton, but the activation of MFO system as well as the damage of the liver by microwave were reduced by green tea supplementation.

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Effects of Shanshuyu (Cornus Officinalis Sieb) tea and Market teas feeding on the Hematology end Liver Function of Rat (산수유 및 차류식이(茶類食餌)가 흰쥐의 간기능과 혈액상(血液像)에 미치는 영향)

  • Joo, Hyun-Kyu;Jang, Dae-Ja
    • Journal of the Korean Society of Food Culture
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    • v.4 no.3
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    • pp.257-264
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    • 1989
  • This study was conducted to develop the Korean traditional tea and investigate the effects of Shan-shu-yu, Gugiga, Ginseng, Younggi and coffee on the hematology of rat. Gugiga, Shan-shu-yu, Younggi and Ginseng tea were adimistrated 3g/day/rat with feeding, respectively. Coffee was adminstrated 1.8g/day/rat. The mixing ratio of mixed tea were 1:1 (w/w). According to the feeding days (10, 20, 30), enzyme activities and chemical components in serum and change in whole blood were determined. 1. The activities of s-GOT and s-GPT of rat administrated Shan-shu-yu, Young-gi, Gugiga, Ginseng and their mixed tea were increased at the normal ranges, and coffee and it's mixed tea were significantly increased other, group (p<0.05). 2. In coffee and it's mixture groups, the content of s-glucose and s-cholesterol were remarkably increased (p<0.05), but in others (except coffee additive group) were decreased than coffee and it's mixture groups. 3. In all groups (except coffee addivite groups), the range of WBC, RBC, Ht and Hb was 7.30-8.00 $({\times}10^3/mm^3)$, 8.18-9.00 ($({\times}10^6/mm^3)$, 50-60 (%) and 16.10-17.40 (g/d), respectively and in strict coffee group, the level of WBC, RBC, Ht and Hb was $8.90{\pm}0.40$, $8.10{\pm}0.37$, $49{\pm}0.38$ and $14.90{\pm}0.44$ (p<0.05), respectively.

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Volatile Flavor Components In a Mixed Tea of Pueraria Radix and Green Tea (녹차와 갈근을 혼합한 차의 휘발성 향기성분)

  • Jeon, Ju-Yeon;Choi, Sung-Hee
    • Journal of Life Science
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    • v.20 no.3
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    • pp.350-355
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    • 2010
  • This study was carried out in order to characterize the flavor of Pueraria radix-green tea. To make a new tea with good flavor and functional properties, Pueraria radix was mixed with green tea. Volatile flavor compounds of Pueraria radix-green tea were extracted by simultaneous distillations and extraction methods using a Likens and Nickerson's extraction apparatus. The concentrated extract was analyzed and identified by GC and GC-MS. Forty-nine compounds including $\beta$-selinene, $\beta$-caryophyllene, hexanal and nonanal were isolated and identified from Pueraria radix. Sixty-four compounds including nerolidol, linalool, linalool oxide and phenylethyl alcohol were isolated and identified from green tea. Eighty-two compounds including linalool, $\delta$-cadinene, limonene, $\beta$-caryophyllene and $\beta$-ionone were isolated and identified from Pueraria radix-green tea.