• Title/Summary/Keyword: Mori Cortex Radicis

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Characterization of anti-oxidative effects of Mori Cortex Radicis

  • Noh, Won-Ki;Park, Jin-Baek;Kim, Sung-Jin
    • Advances in Traditional Medicine
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    • v.10 no.4
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    • pp.271-277
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    • 2010
  • We tested to determine if Mori Cortex Radicis extract has antioxidant activities and its potential mechanism of action was explored. Anti-oxidative effects were tested by measuring free radical and nitric Oxide (NO) scavenging activity, and reducing power. Since iNOS and COX-2 are important enzymes responsible for the production of free radicals in the cell, Mori Cortex Radicis extract was tested as to whether it could inhibit iNOS and COX-2 expression in LPS stimulated Raw cells. 70% methanolic extract of Mori Cortex Radicis exerted significant DPPH free radical and NO scavenging activities. In addition, the Mori Cortex Radicis extract exerted dramatic reducing power with maximal activity observed at 1 mg/ml (11-fold over control). Production of iNOS induced by LPS was significantly inhibited by the Mori Cortex Radicis extract, suggesting it could inhibit NO production by suppressing iNOS expression. COX-2 induced by LPS was also significantly inhibited by the Mori Cortex Radicis extract. The extract contains well known antioxidant components including phenolics, flavonoids and anthocyanin at the concentration of 0.23 mg/g, 42.97 mg/g and 12.08 mg/g, respectively. These results suggest that 70% methanolic extract of Mori Cortex Radicis exerts significant anti-oxidant activity via inhibiting iNOS and COX-2 induction.

Optimization of Cookies Prepared with Mori cortex radicis Powder (상백피 분말 첨가 쿠키의 제조조건 최적화)

  • Park, Young Il;Joo, Nami
    • The Korean Journal of Food And Nutrition
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    • v.27 no.3
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    • pp.522-531
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    • 2014
  • The purpose of this study was to investigate the antioxidant effects of Mori cortex radicis powder and to determine the optimal composite recipe by testing different amount of Mori cortex radicis powder and sugar in cookies prepared with Mori cortex radicis powder. In regard to its antioxident effects, Mori cortex radicis powder had a total phenolic content and DPPH free radical scavenging activity of 149.56 mg GAE/g and $137.77{\mu}g/mL$, respectively. The response surface methodology was used to obtain ten experimental points (including two replicates for Mori cortex radicis powder and sugar) and Mori cortex radicis cookie formulation was optimized using rheology. The results of the sensory evaluation produced significant values for color (p<0.05), texture (p<0.05), sweetness (p<0.01) and overall quality (p<0.05), and the results of instrumental analysis showed significant values in sweetness (p<0.001), redness (p<0.01) and spread ratio (p<0.5). As a result, the optimum formulations obtained by numerical and graphical methods were found to be 16.84 g of Mori cortex radicis powder and 64.42 g of sugar.

Hair growth effects of Mori Cortex Radicis Mixture on the hair of rat (탈모증에 대한 상백피 복합물의 모발성장 효과)

  • 이희삼;윤성중;문윤기;문재유
    • Journal of Sericultural and Entomological Science
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    • v.42 no.2
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    • pp.83-85
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    • 2000
  • This study was carried out to investigate the hair growth effect of Mori cortex radicis mixture on the hair of rat. Cytarabine(50mg/kg) was injected to eight-day-old rats for 7 days. When the mixture of Mori cortex radicis was administered to the rat by route of skin, it promoted the growth of hair. These data suggest that Mori cortex radicis mixture have an effect on the hair growth in cytarabine-induced alopecia rat.

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Pro-Apoptotic Effect of Mori Cortex Radicis in A549 Lung Cancer Cells (상백피가 A549 폐암세포주의 세포사에 미치는 영향)

  • Bae Oh-Sung;Yoo Yeong-Min;Lee Seon-Goo
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.19 no.6
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    • pp.1563-1567
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    • 2005
  • Mori Cortex Radicis is distributed in Northwestern China, northern Asia, northern Europe, North America, and Korea. This extracts drops sugar in bloods and inhibits cyclic AMP phophodiesterase. In this study, we investigated whether Mori Cortex Radicis would cause apoptotic death of A549 lung cancer cells. To examine the apoptotic effect of Mori Cortex Radicis, cytotoxicity assay, DNA fragmentation analysis, caspase-3 activity assay, and Western blotting for caspase-3, caspase-9 and poly(ADP-ribose) polymerase (PARP) and cytochrome c were performed. Treatment of cells with Mori Cortex Radicis was shown to induce cell death in a dose-dependent manner. DNA fragmentation was made in response to Mori Cortex Radicis. The active fragments of caspase-3, caspase-9 and PARP were almost completely induced and cytochrome c was released following exposure to Mori Cortex Radicis. To elucidate the apoptotic mechanisms, RT-PCR and Western blot analyses for the expression of Bcl-2, Bu and Cox-2 were carried out. Treatment with Mori Cortex Radicis was expressed the reduction of Bcl-2 and Cox-2 and the induction of Bax. Especially p21 and p53 were increased prior to untreated control, while cyclin E and cyclin D1 decreased in the cytosol. These results suggest that the effect Mori Cortex Radicis is associated with the cell cycle arrest and pro-apoptotic cell death in A549 lung cancer cells.

Protective Effects of Extracts of Mori Cortex Radicis on Carbon Tetrachloride-induced Hepatotoxicity (사염화탄소에 의해 유발된 간독성에 대한 상백피 추출물의 간보호효과)

  • 김선여;이희삼;류강선;이은주;김영중
    • YAKHAK HOEJI
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    • v.43 no.3
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    • pp.391-396
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    • 1999
  • In order to evaluate the protective effects of extracts of Mori cortex radicis on carbon tetrachloride-induced hepatotoxicity, glutamic oxaloacetic transaminase, glutamic pyruvic transaminase, lactic dehydrogenase, malondiadehyde values and glutathione S-transferase activity were measured in ICR mice. The activities of serum aminotransferase and the hepatic content of lipid peroxide after carbon tetrachloride-treatment were markedly increased than normal control but those levels were significantly decreased by the treatment of butanol fraction of Mori cortex radicis mathanol extract. Glutathione S-transferase activity was decreased by carbon tetrachloride than control, but that also inhibited by the treatment of butanol fraction of Mori cortex radicis methanol extract. These results demonstrate a possible hepatoprotective role of extract role of extract of Mori cortex radicis against ${CCl_4}-induced$ hepatoxicity in vivo.

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Anti-tumor Metastatic Effect and Activation of Innate Immunity by Extract of Mori Radicis Cortex (상백피(桑白皮)의 선천면역 활성화에 의한 항암 효과)

  • Jeong, Jae-Hyuk;Lee, Jin-Moo;Lee, Chang-Hoon;Cho, Jung-Hoon;Jang, Jun-Bock;Lee, Kyung-Sub
    • The Journal of Korean Obstetrics and Gynecology
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    • v.22 no.1
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    • pp.31-40
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    • 2009
  • Purpose: This study was carried out to investigate the anti-tumor metastasis effect and activation of innate immunity by extracts of Mori radicis cortex. Methods: Anti-tumor metastatic experiment was conducted in vitro and in vivo by using colon 26-M3.1 carcinoma cell, L5178Y-R lymphoma cell and HeLa cell. To observe the activation of innate immunity by extracts of Mori radicis cortex, we estimated IL-6, IL-10, IL-12, TNF-${\alpha}$ from peritoneal macrophages. And we evaluated the activation of NK cell by using anti-asialo-GM1 serum. Results: We found that the administration of Mori radicis cortex extracts significantly inhibited tumor metastasis. In an in vitro cytotoxicity analysis, Mori radicis cortex affected tumor cell growth above specific concentration. Mori radicis cortex also stimulated peritoneal macrophage, which was followed by the production of various cytokines such as IL-6, IL-10, IL-12, TNF-${\alpha}$. The depletion of NK cells by anti-asialo GM1 serum partly abolished the inhibitory effect of Mori radicis cortex on tumor metastasis. Conclusion: Mori radicis cortex appears to have considerable activity on the anti-metastasis by activation of innate immunity.

Hair growth Effect of Mori Cortex Radicis extract on Cytarabine-induced alopecia (Cytarabine으로 유도된 탈모증에 대한 상백피 추출물의 육모촉진 효과)

  • 이희삼;문재유
    • YAKHAK HOEJI
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    • v.44 no.1
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    • pp.66-70
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    • 2000
  • This study was designed to examine the effect of Mori cortex radicis extract on cytarabine induced alopecia in young rat. cytarabine (50 mg/kg) was injected to Eight-day-old rats everyday for 7 days. After the injection, Mori cortex radicis extract was spread on the alopecia young rats for 12 days topically. The stimulative effect of hair growth was observed on 12th day of topical application. The stimulative effect of hair growth was best in MeOH extract group. And the same result was obtained in the experiment of hair follicle cross section.

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Purification of Anti-allergic Compound from Mori Cortex Radicis Extract (상백피 추출물 중의 항알러지 활성 성분의 분리)

  • Lee, Eun-Ju;Chae, Ok-Hee;Lee, Moo-Sam;Lee, Hern-Ku;Huh, Hoon
    • YAKHAK HOEJI
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    • v.42 no.4
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    • pp.395-402
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    • 1998
  • Mori Cortex Radicis, the root bark of mulberry tree, has been used in the treatment of bronchial asthma and other lung diseases in traditional medicine. There was a recent repor t that the water soluble part with molecular weight of above 10,000 has anti-allergic activity. Therefore, we intended to isolate and purify the anti-allergic compound from hot water extract of the Mori Cortex Radicis. Crude extract of Mori Cortex Radicis was prepared by hot-water extraction, and anti-allergic compound was further purified by alcohol precipitation, successive ultrafiltration, anion exchange chromatography and gel filtration chromatography. This compound had homogeneity which was shown by the sharp single peak in HPLC chromatogram (TSK-GEL G400OPW column, RI detector). The molecular weight of the compound was estimated as 23Kda on the basis of calibration curve plotted against protein standards. This compound was identified as complex of sugar, protein and lignin (19.2: 5.9: 72.7), and proteolysis could not decrease the anti-allergic activity but mild delignification decreased the activity remarkably. Therefore, we concluded that the anti-allergic compound of Mori Cortex Radicis was a lignin-carbohydrate complex.

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Quality Characteristics and Optimization of Bread with Mori Cortex Radicis Powder Using Response Surface Methodology (상백피 분말 첨가 식빵의 품질특성 및 제조조건 최적화)

  • Chung, Minju;Jeong, Hee Sun;Joo, Nami
    • Journal of the Korean Society of Food Culture
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    • v.28 no.5
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    • pp.512-524
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    • 2013
  • The purpose of this study was to investigate the antioxidative effects of Mori Cortex Radicis powder and to determine the optimal mixing ratio of Mori Cortex Radicis powder and water in the preparation of bread. The optimal sensory composite recipe was determined by producing bread with different levels of Mori Cortex Radicis powder and water. The analysis was performed using response surface methodology and a sensory evaluation was performed with the data. Ten experimental recipes, including two with reference points in the composition, were selected. In terms of the antioxidative effects of Mori Cortex Radicis powder, the $IC_{50}$ for total phenolic content and DPPH free radical scavenging activity were 149.56 GAE/g dry powder and 137.77 /mL respectively. Measurement results of the mechanical properties showed differences in volume (p<0.05), baking loss (p<0.05), yellowness (p<0.01), lightness (p<0.01), redness (p<0.01), hardness (p<0.01) and springiness (p<0.05). The sensory measurements showed significant values for color (p<0.05), appearance (p<0.05), flavor (p<0.01), taste (p<0.01), and overall quality (p<0.01). Overall, based on numerical and graphical methods, the optimal formulation was determined to be 21.16 g of Mori Cortex Radicis powder and 372.47 g of water.

Effects of Jeonglyukdaejosapaetang on the Pulmonary Edema of the Rats Induced by the Herbicide, Paraquat (정역대조사폐탕(大棗瀉肺湯)이 Paraquat로 유발(誘發)시킨 흰쥐의 폐수종(肺水腫)에 미치는 영향(影響))

  • Kang Byung-Ku;Lee Si-Hyeong
    • Herbal Formula Science
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    • v.8 no.1
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    • pp.225-239
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    • 2000
  • Pulmonary edema is a disease involving the principal symptoms: dyspnea, bloody phlegm, asthma, cough, etc. According to oriental medical references, Jeomglyukdaejosapaetang (J.D.T) was efficacious for dropsy, cough, dysnea, etc, so it was thought to be used for remedy of pulmonary edema. Therefore experimental study was performed to investigate the effects of J.C.T on pulmonary edema of the rats induced by the herbicide, paraquat.Thus the survival rats, respiratory rats, lung weights and histopathological view of the lungs of rats were studied. The results are as follows. 1. The survival rates at 72hours of the rats injected with paraquat and treated with J.D.T increased in B group(J.D.T + Cortex Lycii Radicis + Cortex mori). The others were not different with the control. 2. The respiratory rates of the rats which survived 72hours later significantly decreased in B group(J.D.T + Cortex Lycii Radicis + Cortex mori). 3. The lung weights of the rats which survived for 72hours later significantly decreased in B group(J.B.T + Cortex Lycii Radicis + Cortex mori). 4. The histopathological views of the lungs of rats induced by paraquat were seen severe hemorrhage, edema and some broken alveoli in control group. But B group(J.D.T + Cortex Lycii Radicis + Cortex mori) were seen little hemorrhage and interstitial hyperplasia. According to the above results, J.D.T + Cortex Lycii Radicis + Cortex mort is effective on the remedy for pulmonary edema of rats induced by paraquat.

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