• Journal of Korean Association for Spatial Structures
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• v.8 no.5
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• pp.83-93
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• 2008

Space truss is a rational system which forming large span in spatial structure and the steel tube is used well as a structure member in truss system. This study includes coupon test and Stub-column compression test on the structural steel tube. The compression test of Stub-column was performed to characterize and quantify the material characteristic and strength of column. In this study, we also researched the matter of compatibility, in which we compared the experiment formula and the abstract formula by the application of the LSD standard formula, SSRC and ECCS multiple column curve.

• Journal of Korean Society of Steel Construction
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• v.20 no.3
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• pp.437-444
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• 2008

The use of imported structural steel tubes has been increased in domestic construction field because of its low price, but the mechanical properties of those steel tube are not verified exactly. This study includes coupon test and stub - column compression test on the structural steel tube. The compression test of stub - column was performed to characterize and quantify the material characteristic and strength of column compatibility, in which we compared the experiment formula and the abstract formula by the application of the LRFD standard formula and multiple column curve.

• YAKHAK HOEJI
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• v.28 no.1
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• pp.21-23
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• 1984

High performance amino acid analyzing method has been developed for the routine analysis of taurine in preparations. Ion-exchange resin #2619 Hitachi Custom Ion-Exchange Resin, $2.6(I.D.){\times}150$(length)mm was used as column, buffer I, pH 3.3 as mobile phase. The retention time of taurine was 7 minutes. Calibration curve by peak height for standard taurine was linear from 2.5ppm to 25ppm. The reproducibility showed relative standard deviation $\pm$1.9% when analyzed 10 times for standard solution. The samples could be continuously analyzed without regenerating the resin between samples. Five samples were applied to column every 12 min. and then the resin was regenerated for 30 min. during one analyzing cycle time, 90 min. The automatic amino acid analyzer has made it possible to assay multiple samples in a relatively short period of time using the analytical magnetic program card. The high sensitivity and specificity of the analytical column of the automatic amino acid analyzer permits the routine analysis of taurine in preparations.

• Earthquakes and Structures
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• v.23 no.1
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• pp.23-34
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• 2022

This paper presents experimental studies on reinforced concrete moment resisting frames that have engineered cementitious composite (ECC) in plastic hinge length (PHL) of beam/column members and beam-column joints. A two-story frame structure reduced by a 1:3 scale was further tested through a shake-table (seismic simulator) using multiple levels of simulated earthquake motions. One model conformed to all the ACI-318 requirements for IMRF, whereas the second model used lower-strength concrete in the beam/column members outside PHL. The acceleration time history of the 1994 Northridge earthquake was selected and scaled to multiple levels for shake-table testing. This study reports the observed damage mechanism, lateral strength-displacement capacity curve, and the computed response parameters for each model. The tests verified that nonlinearity remained confined to beam/column ends, i.e., member joint interface. Calculated response modification factors were 11.6 and 9.6 for the code-conforming and concrete strength deficient models. Results show that the RC-ECC frame's performance in design-based and maximum considered earthquakes; without exceeding maximum permissible drift under design-base earthquake motions and not triggering any unstable mode of damage/failure under maximum considered earthquakes. This research also indicates that the introduction of ECC in PHL of the beam/column members' detailing may be relaxed for the IMRF structures.

• Journal of Korean Society of Steel Construction
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• v.13 no.3
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• pp.289-299
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• 2001

The purpose of this study was to analyze the characteristics of semi-igid behavior of the steel tubular column to H-beam connection reinforced with exterior square-plate diaphragms and to check the main parameters that affect this behavior. Steel tube connections without interior diaphragm and/or complicated exterior diaphragm show the considerable flexibility due to out of-plane deformation of tube flange. For the exact analysis well-reflected the effect of this flexibility on the overall frame performance. it need to find out the moment-rotation curve function that well trace the result of experiment in the whole region and the function should be simply transformed into an adequate form for the nonlinear analysis program. After collecting several test data same to the connection type considered. we carried out FEM analysis using ANSYS for the assumed beam-to-column connection developed from the simple tension test and the results are compared with experimental values. Based on the parametric study. we proposed the moment-relation curve function and performed the multiple-regression analysis procedure for three parameters consisting of this function with the main geometric parameter of this connection type.

• Analytical Science and Technology
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• v.35 no.2
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• pp.82-91
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• 2022

Grayanotoxin-contaminated honey exhibits toxicity. In this study, a reliable and sensitive liquid-chromatography-tandem-mass-spectrometric method (LC-MS/MS) was developed and validated for the quantitation of grayanotoxin I and grayanotoxin III in honey. The grayanotoxins were extracted from honey via solid phase extraction and separated on a biphenyl column with a mobile phase consisting of 0.5 % acetic acid in water and methanol. Mass spectrometric detection was performed in the multiple-reaction monitoring mode with positive electrospray ionization. The calibration curve covered the range 0.25 to 100 ㎍/g. The intra- and inter-day deviations were less than 10.6 %, and the accuracy was between 94.3 and 114.0 %. The validated method was successfully applied to the determination of grayanotoxins in mad honey from Nepal. The concentrations of grayanotoxin I and grayanotoxin III in 33 out of 60 mad honey samples were 0.75 - 64.86 ㎍/g and 0.25 - 63.99 ㎍/g, respectively. The method established herein would help in preventing and confirming grayanotoxin poisoning.

• Bulletin of the Korean Chemical Society
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• v.34 no.9
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• pp.2760-2764
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• 2013

Geniposide is a biologically active ingredient of gardenia fruit. A liquid chromatography-tandem mass spectrometric method was developed and validated for the determination of geniposide in rat plasma. The plasma samples were pretreated by solid-phase extraction and introduced into a BDS Hypersil $C_{18}$ column ($50{\times}2.1mm$, $5{\mu}m$) for chromatographic separation. The mobile phase consisted of 0.1% formic acid and 0.1% formic acid in acetonitrile, and gradient elution was performed at a flow rate of 0.25 mL/min. For mass spectrometric detection, multiple reaction monitoring was performed via an electrospray ionization source in positive mode. The calibration curve for geniposide was linear ($r^2=0.997$) in the concentration range of $0.005-1{\mu}g/mL$. The intra- and inter-day accuracies and precisions fulfilled the required criteria (${\pm}15%$). The developed method was subsequently used for pharmacokinetic analysis of geniposide after oral administration to rats at a dose of 50 mg/kg. The mean maximum plasma concentration of geniposide was $0.68{\pm}0.29{\mu}g/mL$ at $0.44{\pm}0.13h$, and the mean area under the plasma concentration versus time curve was $1.46{\mu}g{\cdot}h/mL$.

• Archives of Pharmacal Research
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• v.27 no.12
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• pp.1202-1206
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• 2004

A rapid, sensitive and selective liquid chromatography-tandem mass spectrometric (LC-MS/ MS) method for the determination of lithospermic acid B (LSB) in rat serum was developed. LSB and internal standard, 7-hydroxy-3-phenyl-chromen-4-one (HPC) were extracted from rat serum with methyl-tert-butyl ether at acidic pH and analyzed on a Luna $C_8$ column with the mobile phase of acetonitrile-ammonium formate (10 mM, pH 6.5) (50:50, v/v). The analytes were detected using a negative electrospray ionization tandem mass spectrometry in the multiple- reaction-monitoring mode. The standard curve was linear $(r^2 = 0.997)$ over the concentration range of 10.0-500 ng/mL. The coefficient of variation and relative error for intra- and interassay at three QC levels were 1.1~6.2% and -10.3~-2.7%, respectively. The recovery of LSB from serum sample ranged from 73.2 to 79.5%, with that of HPC (internal standard) being 75.1 %. The lower limit of quantification for LSB was 10 ng/mL using 50 ${\mu}L$ of serum sample.

• Proceedings of the Ginseng society Conference
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• 1980.09a
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• pp.59-69
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• 1980

A new HPLC-method for separation and quantitative determination of ginsenosides in Panax ginseng, Panax quinquefolium and in pharmaceutical drug preparations is elaborated. A reversed-phase-system with ${\mu}Bondapak\;C_{18}$ column (3.9 mm $I.D.{\times}30\;cm$) using acetonitrile-water (30:70) 2 ml/min and acetonitrile-water (18:82) 4 ml/min is suitable for the base-line separation of $Rb_1,\;Rb_2,\;Rc,\;Rd,\;Rf,\;Rg_2,\;respectively\;Re,\;Rg_1$ in 30 minutes. The ginsenosides are directly detected at 203 nm (without derivatization) with the LC-55 or LC-75 spectrophotometer (Perkin-Elmer) at $100\%$ transmission. Detection limit is 300 ng at a signal-to-noise ratio of 10:1. The ginsenosides-peak identification is carried out with HPTLC (high performance thin layer chromatography), with MIR-IR (multiple internal reflection-IR-spectros-copy) and with FD-MS (field desorption mass spectrometry). The calibration curve of each ginsenoside has a correlation coefficient very near to 1. Relative standard deviation for quantitative determinations depends upon the amount of ginsenosides and is approximately 1\%$ for ginsenoside contents of 1\%$. This method is adaptable for routine analysis in quality control laboratories.

• Journal of the Korean Geotechnical Society
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• v.38 no.6
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• pp.17-28
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• 2022

The effect of the soil-structure interaction (SSI) on has been recently evaluated in shaking table tests. However, most of these tests were conducted on single-degree-of-freedom (SDOF) superstructures and a single-pile. This study investigates the inertial interaction effect of a multi-degree-of-freedom (MDOF) superstructure system with a group piles on a large-scale shaking table test. Whereas the SDOF superstructure system shows a single-frequency amplification tendency, the MDOF superstructure system exhibited amplification tendencies of the acceleration phase and frequency responses for multiple frequencies. In addition, the amplification phenomenon between the footing and the column-type superstructure exceeded that between the footing and the wall-type superstructure, indicating a greater inertial interaction effect of the column-type superstructure. The relationship between shear force and inertial force, the relative vertical and horizontal displacements on the footing was figured out. Also, the ananlysis of dynamic p-y curve at each depth was conducted. In summary, the MDOF and SDOP superstructure systems exhibited different behaviors and the column-type superstructure exerted a higher interaction effect than the wall-type superstructure.