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Genotoxicity Evaluation of the Glycyrrhiza New Variety extract (감초 신품종 추출물의 유전독성 평가)

  • Young-Jae Song;Dong-Gu Kim;Jeonghoon Lee;Wonnam Kim;Hyo-Jin An;Jong-Hyun Lee;Jaeki Chang;Sa-Haeng Kang;Yong-Deok Jeon;Jong-Sik Jin
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2021.04a
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    • pp.67-67
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    • 2021
  • The genus Glycyrrhiza (Licorice) has been used as an oriental herbal medicine for a long time in Asian countries. Wongam (WG), which is Glycyrrhiza new variety, have been developed to improve limitation of licorice including low productivity, environmental restriction and insufficient components by Korea Rural Development Administration. To using WG as a herbal medicine, it is important to reveal the adverse effects in health. In this study, we evaluated the genotoxicity test of WG extract through in vitro bacterial reverse mutation (AMES) assay, in vitro chromosomal aberration assay and in vivo mouse bone marrow micronucleus assay. When compared with the control, WG extract with or without the S9 mix showed no genotoxicity in the AMES assay up to 5000 ㎍/plate and in the chromosomal aberration assay up to 1100 ㎍/ml. In micronucleus assay, no significant increase in the number of micronucleated polychromatic erythrocytes or in the mean ratio of polychromatic to total erythrocytes up to 5000 mg/kg/day for 2 days. The present study demonstrated that WG extract is safe and reliable herbal medicine since no detectable genotoxic effects at least under the conditions of this study.

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Assessment of the Single Oral dose Toxicity of Glycyrrhiza New Variety Extract in Sprague-Dawley Rats (Sprague-Dawley rats에서 감초 신품종 추출물의 단회투여 독성 평가)

  • Dong-Gu Kim;Jeonghoon Lee;Wonnam Kim;yo-Jin An;Jong-Hyun Lee;Jaeki Chang;Sa-Haeng Kang;Young-Jae Song;Yong-Deok Jeon;Jong-Sik Jin
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2021.04a
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    • pp.65-66
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    • 2021
  • Glycyrrhiza species (Licorice) are one of the most commonly used medicinal plants in Asian countries such as China, India and Korea. It has been traditionally used to treat many disease including cough, cold, asthma, fatigue, gastritis and respiratory tract infections. Glycyrrhiza new variety, Wongam (WG), have been developed by Korea Rural Development Administration and revealed several pharmacological effects. However, limited data are available on the potential adverse effects of the WG. Here, we evaluated the general toxicity of the WG extract through single oral dose toxicity study in Sprague-Dawley rats. After single oral dose administration, there was no mortality up to 5000 mg/kg during experiment period. In addition, there was no clinical signs including body weight change, gross findings and necropsy findings up to 5000 mg/kg during experiment period. To conclude, the no-observed-adverse-effect level (NOAEL) of WG was higher than 5000 mg/kg and no target organs were identified in male and female Sprague-Dawley rats.

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Effects of Ridge Width on Growth and Yield of Proso Millet (Panicum miliaceum L.) in Paddy-Upland Rotation Field (답전윤환지에서 이랑너비에 따른 기장의 생육특성 및 수량)

  • Yoon, Dong-Kyung;Oh, Seung-Ka;Lee, Byung-Jin;Chun, Hyun-Sik;Jung, Ki-Youl;Kang, Hang-Won;Jeon, Seung-Ho;Cho, Young-Son
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.60 no.2
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    • pp.217-223
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    • 2015
  • This study was conducted to serve as a basis for establishing a stable production of proso millet on the rotational paddy-upland field by looking out the physicochemical and moisture characteristics of soil and the growth characteristics of proso millet by ridge width. Plant height showed Manhongchal was the smallest 71.3 cm in 240 cm wide of ridge in the first year, Ibeakchal was the highest 69.7 cm in 60 cm wide in the second year, Hwanggeumchal was the highest 72.8 cm in 60 cm wide in the first year. The retention time of the excess water in the soil during cultivation was extended wider the width of ridge. The variation width of the water content was higher by the wider the width of ridge. Yield components showed the longest ear length were 35.1 cm of Manhongchal, 34.8 cm of Ibeakchal in 60 cm wide of ridge in the first year. As the width of ridge extended, ear weight of all variety increased. 1,000 seed weight of the 60 cm wide of ridge was Manhongchal 6.8%, Ibeakchal 46.2% heavier than the 240 cm wide of ridge. Yield showed Manhongchal 221, Ibeakchal 223, Hwanggeumchal $225kg{\cdot}10a^{-1}$ in 60 cm wide of ridge in the first year. The similar pattern of amount showed Manhongchal 278, Ibeakchal 221, Hwanggeumchal $200kg{\cdot}10a^{-1}$ in 60 cm wide of ridge in the second year. This showed Manhongchal 103%, Ibeakchal 119%, Hwanggeumchal 85.2% was more than the 240 cm wide of ridge.

Influence of Land Use on the Pollution Load in the Saemangeum Basin (새만금 유역에서 토지 이용이 오염부하에 미치는 영향 평가)

  • Lee, Deog-Bae;Kim, Jong-Cheon;Lee, Kyung-Bo;Kim, Jong-Gu;Park, Chan-Won
    • Korean Journal of Soil Science and Fertilizer
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    • v.39 no.4
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    • pp.237-244
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    • 2006
  • The SMG project has been driven to secure food and water resources by closing of the SMG dyke for the preparation of the unification of Korean peninsular. It was investigated for pollution loads, land use distribution and water consumption for environmental assessments in two watersheds, the Mankyeong River (MK) and the Dongjin River (DJ) to assess the role of agricultural land on the alleviation of pollution loads to the SMG basin. It is needed to give the priority in managing pollution sources to conserve freshwater in the Saemangeum (SMG) basin after the completion of the SMG reclamation from tideland. The MK has $700million\;m^3$ water of which 14.1% were used for living, 73.6% for agriculture and 12.3% for industry. The DJ has $505million\;m^3$ water of which 3.0% for living, 94.5% for agriculture and 2.5% for industry. As compared to proportion of each land of total area, agricultural land was 1.4 times larger, livestock farming 7 times larger, forest 0.74 times smaller, and built-up area 0.67 times smaller in DJ watershed than in MK watershed. Pollution sources in MK and DJ watersheds were originated at a higher proportion from population including the sewage disposal and a livestock farming area rather than from the land. Water consumption and land use distribution influenced the water quality of the rivers; DJ watershed had far lower value of electric conductivity, $BOD_5$, TN and TP than MK watershed. A large proportion of paddy field also influenced to reduce pollute loadings after rainfall; DJ watershed, which has a relatively large area of paddy fields, had a far lower delivery load after rainfall than MK watershed even though DJ watershed had large livestock farming area. As paddy fields was irrigated by Iksancheon water, 37% of nitrogen, 50% of phosphates and 14.0% of $BOD_5$ was removed by the paddy field just after flowing 150 meter, and rice plants could remove TN 100.0 kg, $P_2O_5$ 24.0 kg, and $K_2O$ 119.2 kg per hectare at harvest by irrigation of Iksancheon water. Conclusively, rice paddy fields played a positive role to conserve the water quality in the Iksancheon watershed.

Physicochemical Properties of Breeding Lines of Colored Barleys (유색보리 육성계통의 이화학적 특성)

  • Choi, Jae-Seong;Park, Soo-Jin;Joung, Yong-Myeon;Kim, Jung-Gon;Won, Mi-Hee;Kang, Myung-Hwa
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.spc1
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    • pp.125-132
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    • 2006
  • The below is the results of physicochemical analysis of 40 breeding lines of colored barley (CB) whose systems are different Water content of CB No. 24 showed the lowest value of 7.4% while CB No. 9 showed the highest value of 10.8%. Crude protein of CB $9.7{\sim}12.9%$ range was found. Crude fat content of CB No. 6 showed the highest value of 4.35% while CB No. 34 showed the lowest of 1.35%. Crude ash content of CB No. 31 showed the lowest value of 1.20%. Ca content of CB No. 10 showed the highest value of 717.50 mg% while general barley showed the lowest value of 442.82 mg%. Mg content of CB No. 10 showed the highest value of 1320.00 mg%. Cu content of CB No. 20 showed the lowest value of 2.20 mg% while CB No. 33 showed the highest value of 6.25 mg%. K content of CB No. 20 showed the lowest value of 723.24 mg% while CB No. 1 showed the highest value of 1002.50 mg%. Mn content of CB No. 28 showed the lowest value of 31.72 mg% while general barley showed the highest value of 94.56 mg%. ${\beta}-Glucan$ content of CB No. 25 showed the lowest value of 5.20 mg% while CB No. 28 showed the highest value of 4.46 mg%.

Flowering Responses and Floral Characteristics of Ipomoea batatas Varieties (고구마 품종별 개화성 및 화기특성)

  • Ahn, Young-Sup;Chung, Mi-Nam;Lee, Joon-Seol;Kim, Young-Guk;An, Tae-Jin;Min, Kyung-Soo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.54 no.4
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    • pp.433-440
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    • 2009
  • Korean sweetpotato, Ipomoea batatas, varieties were examined for their flowering response as a fundamental research to improve breeding efficiency by hybridization of this crop. Under 10.0~10.5 hours short day condition, self-rooted plants of three varieties such as Shinmi, Hongmi and Hwangmi flowered sparsely or moderately, and those of fourteen varieties including Eunmi flowered very sparsely. Under 10.6~12.0 hours day length condition, self-rooted plants of three varieties such as Shinmi, Hongmi and Hwangmi flowered very sparsely. Under the day length longer than 12 hours, none of the tested plants flowered. In all the sweetpotato plants grafted on morning glory, flowering occurred with varying degrees by varieties and day lengths. Under 10.0~10.5 hours day length, thirty two varieties including Hongmi flowered moderately or profusely, and one variety such as Mokgye 1 flowered sparsely. Under 10.6~12 hours day length, twenty three varieties including Sinchunmi flowered moderately or profusely, nine varieties including Jeungmi flowered sparsely, and Mokgye 1 didn't flower at all.

Pro-apoptotic and Anti-adipogenic Effects of Proso Millet (Panicum miliaceum) Grains on 3T3-L1 Preadipocytes (기장(Panicum miliaceum)의 마우스 3T3-L1 세포에 대한 에폽토시스 유발 및 지방세포형성 억제 효능)

  • Jun, Do Youn;Lee, Ji Young;Han, Cho Rong;Kim, Kwan-Pil;Seo, Myung Chul;Nam, Min Hee;Kim, Young Ho
    • Journal of Life Science
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    • v.24 no.5
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    • pp.505-514
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    • 2014
  • To examine the anti-obese activity of miscellaneous cereal grains, 80% ethanol extracts from eight selected miscellaneous cereal grains were compared for their cytotoxic effects on 3T3-L1 murine preadipocytes. The ethanol extract of proso millet exhibited the highest cytotoxicity. Further fractionation of the ethanol extract with methylene chloride, ethyl acetate, and n-butanol showed that the cytotoxicity of the ethanol extract was mainly partitioned into the butanol fraction. As compared with differentiated mature adipocytes, 3T3-L1 preadipocytes were more susceptible to the cyctotoxicity of the butanol fraction. When each organic solvent fraction (25 ${\mu}g/ml$) was added during the differentiation period for 6 days, the cell viability was not affected significantly except for the butanol fraction, but the intracellular lipid accumulation declined to a level of 81.5%~50.3% of the control. The Oil Red O staining data also demonstrated that the ethanol extract as well as the butanol fraction could inhibit the differentiation of 3T3-L1 preadipocytes into mature adipocytes. The presence of the butanol extract during the induced adipocytic differentiation also resulted in a significant reduction in the expression levels of critical adipogenesis mediators $(C/EBP{\alpha}$, $PPAR{\gamma}$, aP2, and LPL) to a barely detectable or undetectable level and the cells retained the fibroblast-like morphology of 3T3-L1. In 3T3-L1 cells, the cytotoxicity of the butanol fraction (50-100 ${\mu}g/ml$) was accompanied by mitochondrial membrane potential (${\Delta}{\psi}m$) loss, caspase-3 activation, and PARP degradation. Taken together, these results indicate that proso millet grains possess pro-apoptotic and anti-adipocytic activities toward adipocytes, which can be applicable to prevention of obesity.

Changes of Feed Quality at Different Cutting Dates among Five Winter Cereals for Whole-Crop Cereal Silage in Middle Region (중부지역에서 총체맥류의 예취시기별 사료가치 변화)

  • Ju, Jung-Il;Lee, Joung-Jun;Park, Ki-Hun;Lee, Hee-Bong
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.29 no.3
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    • pp.187-196
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    • 2009
  • The objectives of this study were to evaluate the different cutting dates on the changes of feed quality among five cereals (barley, wheat, rye, triticale and oat) for whole crop silage. Field trials were conducted at paddy field in Yesan, Chungnam Province and the aerial parts were clipped 10 days from 15 March to 15 June. Changes of acid detergent fiber (ADF) content in relation to different cutting dates was described by a quadratic curve for 5 winter cereals crops. ADF content reached a maximum at 5 days after heading in barley cultivar 'Youngyang', 7 days in wheat 'Keumkang', 18 days in rye 'Gogu', 1 days in triticale 'Shinyoung' and 10 days in oat 'Samhan'. Neutral detergent fiber (NDF) content were linearly increased as growing after overwintering and stagnated or slightly decreased after heading. The crude protein were linearly decreased throughout the growth period of 5 whole crop cereals. Digestible dry matter (DDM) content were decreased from early stages to heading and subsequently increased as grain filling. Relative feed value (RFV) for 5 crops were decreased as growing and subsequently increased as grain filling after heading. Barley cultivar for only forage use 'Youngyang' were lower at ADF and NDF content and higher at DDM and RFV after heading than those of other cereals for forage use. So, barley for whole crop silage was a good crop with high feed quality and high proportion of spikes compared with other winter cereal crops. Wheat cultivar for grain 'Keumkang' were higher at crude protein than those of other four cereals from overwintering to maturing and were higher at DDM and RFV after heading than those of rye, triticale and oat. Rye cultivar with cold tolerant and high fresh yielding 'Gogu' were highest at ADF and NDF content and lowest at DDM content and RFV. So, rye was a crop with low quality for forage use compared to other winter cereal crops. Triticale cultivar with flourishing and high yielding 'Shinyoung' was intermediated between barley and rye, and were linearly increased at DDM yield by different cutting dates. Oat cultivar with cold tolerant and high tillering 'Samhan' were lower at ADF and NDF content and higher at crude protein before heading, but after heading, there are not especially advantages compared to barley, wheat or triticale.

Changes of Mating Type Distribution and Fungicide-resistance of Phytophthora infestans Collected from Gangwon Province (강원지역 감자 역병균 Phytophthora infestans의 교배형 및 약제저항성 변화)

  • Park, Kyeong-Hun;Ryu, Kyoung-Yul;Yun, Jeong-Chul;Jeong, Kyu-Sik;Kim, Jeom-Soon;Kwon, Min;Kim, Byung-Sup;Cha, Byeong-Jin
    • Research in Plant Disease
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    • v.16 no.3
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    • pp.274-278
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    • 2010
  • Potato late blight caused by Phytophthora infestans was the most constrain disease at potato cultivation areas. The mating type distribution and fungicides response of P. infestans were investigated to elucidate the changes of pathogen from Gangwon province. On the fungal isolates in 2006, 58.7% were A1 mating type and 41.3% were A2 mating type. In 2007, A1 mating type isolates increased to 93.3% and A2 mating type isolates were collected from Jinbu areas as much as 6.7%. About 234 isolates analysed for metalaxyl response, the results was resistance 73.7%, intermediate 18.8% and sensitive 7.5% in 2006. And it was resistance 59.4%, intermediate 4.0% and sensitive 36.6% in 2007. It meant that mating type distribution and fungicide response were very different over the collection sites. Minimal inhibition concentration (MIC) of dimethomorph examined with 126 isolates in 2006 and 106 isolates in 2007. MIC over $1.0\;{\mu}g/ml$ was 56.3% in 2006 and it was 3.8% in 2007. The average $EC_{50}$ value of P. infestans was $0.37\;{\mu}g/ml$ in 2006, but it decreased to $0.12\;{\mu}g/ml$ in 2007. Fungicides response and pathogenesis of P. infestans should be monitored continuously to enhance the chemical efficacy at potato fields.

Changes in Lignan Content and Antioxidant Activity of Fermented Sesame (Sesame indicum L.) by Cultivars (생물전환에 의한 품종별 참깨 발효물의 Lignan 함량과 항산화 활성 변화)

  • Jung, Tae-Dong;Shin, Gi-Hae;Kim, Jae-Min;Oh, Ji-Won;Choi, Sun-Il;Lee, Jin-Ha;Cho, Myoung-Lae;Lee, Sang Jong;Heo, In Young;Park, Seon Ju;Kim, Sung-Up;Jung, Chan-Sik;Lee, Ok-Hwan
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.1
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    • pp.143-148
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    • 2016
  • This study investigated the lignan content, total phenol content, and antioxidant activities [2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and oxygen radical absorbance capacity (ORAC)] of fermented sesame by cultivars. The results showed that the lignan contents of fermented and non-fermented sesame ranged from 2.35~6.58 mg/g and 2.17 to 6.58 mg/g, respectively. The highest total phenol contents of fermented and non-fermented sesame were 51.90 mg gallic acid equivalent (GAE)/g and 25.94 mg GAE/g, respectively. DPPH radical scavenging and ORAC value ranged from 37.95 to 82.57% and from 172.34 to $1,067.80{\mu}M$ TE/g in non-fermented sesame and fermented sesame, respectively. Fermented sesame had higher lignan content, total phenol content and antioxidant activities. than those of non-fermented sesame. Fermented sesame subjected to bioconversion showed increased lignan content and high antioxidant activity.