• Title/Summary/Keyword: Newcastle disease virus

Search Result 94, Processing Time 0.139 seconds

Studies on Histological Changes of Bursa of Fabricius in Chicken Treated with Thyroxine II. Effect of Thyroxine on Antibody Production (갑상선(甲狀腺) 호르몬이 닭의 Fabricius낭(囊)에 미치는 조직학적변화(組織學的變化)에 관(關)한 연구(硏究) II. 갑상선(甲狀腺) 호르몬이 항체산생(抗體産生)에 미치는 영향(影響))

  • Kim, Soon Bok;Lee, Cha Soo
    • Korean Journal of Veterinary Research
    • /
    • v.20 no.2
    • /
    • pp.99-104
    • /
    • 1980
  • The effects of thyroxine (TX) or propylthiouracil (PPT) administration on the antibody forming activity agains t sheep red blood cell (SRBC) and Newcastle disease virus (NDV) were studied by using of hemagglutination and hemagglutination-inhibition techniques. Antibody titers to both SRBC and NDV increased significantly in the TX-treated group, whereas decreased in the PPT-treated group, compared with control. When TX was administered after antigen inoculatioon, antibody forming activity was significantly enhanced, compared with the TX administration before antigen inoculation.

  • PDF

수의학강좌 II: 최신 양계 호흡기 질병 동향 및 대처방안

  • Song, Chang-Seon
    • Journal of the korean veterinary medical association
    • /
    • v.46 no.8
    • /
    • pp.726-735
    • /
    • 2010
  • 전염성기관지염 바이러스(infectious bronchitis virus: IBV), 조류 뉴모 바이러스(Avian pneumovirus: APV), 뉴캣슬병 바이러스 (Newcastle disease virus: NDV), 조류인플루엔자 바이러스(avian influenza virus: AIV) 전염성 후두기관염 바이러스 (infectious laryngotracheitis virus: ILTV)는 닭의 호흡기에 직접 감염하여 호흡기질환을 일으키는 대표적인 바이러스로 알려져 있다. 그 밖에 아데노바이러스(adenovirus)와 레오바이러스 (reovirus)도 닭의 상부호흡기에 침투하여 피해를 입히는 이차적 원인체로 작용할 수 있다. 이들중 APV와 ILTV는 닭의 호흡기도에 국한되어 증식하지만 IBV, NDV, AIV의 경우 호흡기도 이외의 장기에서 증식이 가능하여 그 피해가 다양하게 나타나 문제 시 되기도 한다 (예: 산란장기 및 신장 (IBV), 소화기 (NDV, IBV, AIV), 중추신경계 (NDV, AIV)). 이외에도 상당수의 감염성 질환이 닭의 호흡기에 영향을 미칠 수 있으나, 해당 농장의 호흡기 피해가 어떤 질병에 의한 것인지 명확히 파악하지 못한 채 단순 항생제 처방에만 의지하는 경우가 많은 것이 현실이다. 따라서 국내 양계농가에서 문제시되는 주요 호흡기 질병과 이들의 감수성을 증대시키는 요인을 파악하는 것이 필요하고, 호흡기 질병의 피해에 대한 재인식과 아울러 호흡기 질병 피해 감소를 위한 진단과 예방노력이 하루속히 정착되어야 할 것이다. 본지에서는 대표적인 호흡기 질병 세가지(전염성기관지염, 조류뉴모바이러스감염증, 뉴캣슬병)의 최근 발생동향과 그 대처방안에 대하여 소개하고자 한다.

  • PDF

IMPROVEMENT AND UTILIZATION OF GENETIC RESOURCES IN NATIVE CHICKEN : RECIPROCAL CROSS BETWEEN TAIWAN COUNTRY CHICKEN AND SINGLE COMB WHITE LEGHORN

  • Lee, Yen-Pai;Huang, Hwei-Huang
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.2 no.2
    • /
    • pp.103-114
    • /
    • 1989
  • Reciprocal crosses were conducted between three strains of Taiwan Country chickens, developed in the National Chung-Hsing University, and two strains of Single Comb White Leghorns, developed in the Taiwan Livestock Research Institute. Traits studied were growing performances, laying performances, egg quality traits and traits concerning disease resistance, including resistance to Marek's disease virus and immune responses to Newcastle disease virus vaccine and to sheep red blood cell. Results indicated that laying performances of Taiwan country chickens were much inferior to White Leghorns, but they matured earlier, their eggs had better shell strength and larger proportion of yolk, and their general disease resistance was much better than White Leghorns. Heterosis were found in laying performances and egg quality traits. The heterosis in laying traits was so large that the hybrid laid as many eggs and as large eggs as did pure strains of White Leghorns. Strategies on the improvement of native chickens and the utilization of genetic merits of native chickens were also discussed.

Changes of maternal antibodies in chicks vaccinated to breeder against infectious bronchitis, infectious Bursal disease, and Newcastle disease virus (모계의 전염성기관지염, 전염성 F낭병 및 뉴캣슬병 백신투여에 따른 모체이행 항체의 변동)

  • 고원석;김태중;이정원;서이원;송희종;오언평
    • Korean Journal of Veterinary Service
    • /
    • v.21 no.2
    • /
    • pp.133-139
    • /
    • 1998
  • Serum samples collected from 30 breeders and their progeny 30 chicks. The antibodies against infectious bronchitis(IB), infectious bursal disease (IBD) and Newcastle disease(ND) viruses were detected by ELISA using commercial ELISA kit. The breeders were vaccinated against IB, IBD and ND viruses according to general vaccination program. Geometric mean titers(GMT) of ELISA were monitored from 1-day old to 17-day old chicks and compared with breeder chickens. The GMT of ELISA to IB, IBD and ND were declined half level of the breeder antibody titer at 6-, 8- and 7-day old. And, the GMT of ELISA to IB, IBD and ND were declined than that of protective titer at 6-, 1-, and 4-day old. Thereafter, the GMT of ELISA was declined and disappeared according to ages of chicks. Taken together, this study led to conclusion that time-course of maternal antibody titers of chicks from vaccinated breeders, and this is very important data for vaccination to chicks.

  • PDF

Immunohistochemistry and RT-PCR for pathogenesis of Newcastle disease in chickens (닭 뉴캣슬병의 발병기전 규명을 위한 RT-PCR 및 면역조직화학적 연구)

  • 이민권;진영배;문운경;김순복
    • Korean Journal of Veterinary Service
    • /
    • v.27 no.1
    • /
    • pp.63-73
    • /
    • 2004
  • The present experiment was carried out to study the pathogenesis of Newcastle disease(ND), ND virus (NDV) antigens and genes in various organs from NDV inoculated chickens were detected by immunohistochemistry and RT-PCR. Immunohistochemically, NDV antigens were detected in the spleen, thymus, cecal tonsil, proventriculus, trachea and lungs at 12 hour post-inoculation (hpi). Viral antigens were localized mainly in the cytoplasm of lymphocytes and macrophages. After 48 hpi, clinical findings of the affected chickens were open-mouth breathing, conjunctivitis, watery diarrhea and edema around the eye and neck. After 72 hpi, chickens showed muscular tremor, paralysis of the legs and wings, and coma. Histopathological results consist of multi-focal necrosis with hemorrhages in lymphoid aggregates of the intestinal tracts, necrosis of the lymphoid tissues, neuronal degeneration and necrosis, and perivascular cuffing. Using RT-PCR, virus genes were detected in the spleen and proventriculus at 48 hpi, and in the brain at 60 hpi.

Production of Newcastle vaccine using continuous mammalian cells

  • Gwak, Il-Yeong;Choe, Yeon-Suk;Jeong, Yeon-Ho;Jeon, Gye-Taek;Kim, Ik-Hwan
    • 한국생물공학회:학술대회논문집
    • /
    • /
    • pp.281-284
    • /
    • 2002
  • Specific pathogen free (SPF) eggs have been used to produce live vaccines. however, their application causes many problems such as cost, space and waste disposal. The substitution of mammalian cells for SPF eggs offers a desirable system of vaccine production. In this study, mammalian cells were tested for the infection of Newcastle disease virus (NDV). As a result, DF-I and MDBK cells showed high virus productivity compared to the other mammalian cells. For the highest productivity of NDV, the optimal multiplicity of infection (M.O.I.) in DF-I or MDBK cells was determined to be 0.2 or 0.5 M.O.I., respectively.

  • PDF

Seroprevalance of Newcastle Disease Virus in Wild Birds in Korea (국내 야생 조류에서의 뉴캣슬병 바이러스 항체분포율 조사)

  • Choi, Kang-Seuk;Jeon, Woo-Jin;Kye, Soo-Jeong;Yoon, Soon-Seek;Jeong, Woo-Seog;Kim, Ji-Ye;Kwon, Jun-Hun
    • Korean Journal of Poultry Science
    • /
    • v.39 no.2
    • /
    • pp.97-104
    • /
    • 2012
  • Newcastle disease virus (NDV) infects a variety of birds with a wide range of clinical signs from asymptomatic to severe. During a 10-month period in 2011, a total of 1,024 sera from wild birds including 42 species of birds in 8 orders were collected and the seroprevalence of NDV in wild birds was evaluated by hemagglutination inhibition (HI) test. Evidence of NDV infection was observed in 12.6% (129/1,024) of wild birds with a maximum prevalence reported in Mandarin duck (27.8%, 32/115) followed by Mallard duck (20.8%, 57/274), Spot-billed duck (11.9%, 36/303), Pintail (2.9%, 1/34), Black-tailed gull (2.9%, 1/34), White-fronted goose (1.8%, 1/56) and Common teal (1.4%, 1/69). None of the other 35 species of wild birds were antibody-positive for NDV. Mandarin duck, Mallard duck and Spot-billed duck showed high sero-prevalance of 12.2% to 42% during winter season (November to March). Our results indicate that Mandarin duck, Mallard duck and Spot-billed duck might be natural reservoirs for NDV in Korea and the prevalence of NDV infection in wild birds displayed a seasonal pattern with high prevalence of NDV in winter season (November to March).

Comparative Pathology of chickens Experimentally Inoculated with Virulent Viscerotropic Newcastle Disease Viruses isolated in Korea (강병원성 뉴캣슬병 바이러스 한국분리주의 SPF 닭 접종에 따른 병리학적 변화 비교)

  • I. P. Mo;Y. K. Kwon;M. G. Han;H. W. Seong
    • Korean Journal of Poultry Science
    • /
    • v.28 no.2
    • /
    • pp.99-106
    • /
    • 2001
  • Pathologic changes and distribution of viral antigen as determined by immunohistochemistry were compared among 4-wk-old specific-pathogen free (SPF) chickens inoculated intratracheally with velogenic vis-cerotropic Newcastle disease virus isolated in Korea. Although the pattern of organ involvement and severity of lesion was different among chickens infected with different velogenic viscerotropic Newcastle disease (VVND) viruses, the pathological types of lesion was similar among the chickens. Severe lymphocytic necrosis and depletion were main histologic lesions in the immune related organs such as thymus, Fabricius bursa and spleen. The frequency of IP positive staining was variable depends on the types of tissues but not types of the kinds of VVND viruses infected. Brain, Fabricius bursa, thymus, cecal tonsil and trachea were IP positive with fairly high frequency and spleen, lung, proventriculus, intestine, pancreas, liver, kidney, heart and Harderian gland were with relatively low frequency. These results suggest that histologic evaluation and viral antigen specific immunohistochemical staining methods to determine virus distribution will be useful for pathogenic study of velogenic viscerotropic Newcastle disease virus infection in chicken.

  • PDF

The Antiviral Effects of Areca catechu L. Extract (빈랑 추출물의 새로운 항바이러스 활성)

  • Lee, Doseung;Boo, Kyung Hwan;Kim, Young Cheon;Lee, Jin-Man;Kang, Seungtae;Lee, Wang Shik;Riu, Key Zung;Lee, Dong-Sun
    • Korean Journal of Food Science and Technology
    • /
    • v.46 no.2
    • /
    • pp.245-248
    • /
    • 2014
  • Trafficking of viral glycoproteins to the cell surface results in syncytium formation in baby hamster kidney (BHK) cells infected with Newcastle disease virus (NDV). An extract from the medicinal Areca catechu L plant inhibited not only syncytium formation, but also trafficking of the hemagglutinin-neuramidase (HN) glycoprotein to the cell-surface. The viral glycoprotein was processed within the endoplasmic reticulum during transit to the cell membrane. Fungal extracts showed inhibitory activities ($IC_{50}10{\mu}g/mL$) against ${\alpha}$-glucosidase. These results suggested that A. catechu L. extracts inhibited the cell-surface expression of NDV-HN glycoprotein without significantly affecting HN glycoprotein synthesis in NDV-infected BHK cells.

Preliminary Studies on Production of Porcine Leucocyte Interferon (돼지 Leucocyte Interferon 생산(生産)에 대한 기초연구(基礎硏究))

  • Jun, Moo Hyung;Chung, Un Ik;Park, Bong Kyun;Kim, Kyo Joon;Lee, Hun Jun
    • Korean Journal of Agricultural Science
    • /
    • v.12 no.1
    • /
    • pp.153-161
    • /
    • 1985
  • As a preliminary step for production of large quantities of porcine leucocyte interferon(Por IFN-${\alha}$), various factors such as the sources of leucocytes, inducers and culture conditions were investigated. In addition, an assay system for the potency of porcine interferon was developed in a micro-system by using porcine kidney cell line, PK-15, and vesicular stomatitis virus. By the experiments, it was found that the porcine leucocyte interferon was synthesized more effectively in the leucocyte suspension with the higher viability and the lesser erythrocyte contamination. Regarding cell sources, the peripheral leucocytes produced a consistently higher unit of interferon than the spleen cells. When the efficiency of inducers was compared, the Sendai virus at the concentration of 125 HA unit/ml was found to be more effective for porcine leucocyte interferon production than the Newcastle disease virus. Average potency of 9 batches of the crude interferon measured by the established assay system was 1,200 unit/ml.

  • PDF