• Asian-Australasian Journal of Animal Sciences
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• 제26권6호
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• pp.838-844
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• 2013

This study was conducted to evaluate chemical composition, nitrogen-corrected true metabolizable energy (TMEn) and true amino acids digestibility of corn distillers dried grains with solubles (DDGS) produced in China. Twenty five sources of corn DDGS was collected from 8 provinces of China. A precision-fed rooster assay was used to determine TMEn and amino acids digestibility with 35 adult cecectomized roosters, in which each DDGS sample was tube fed (30 g). The average content of ash, crude protein, total amino acid, ether extract, crude fiber and neutral detergent fiber were 4.81, 27.91, 22.51, 15.22, 6.35 and 37.58%, respectively. TMEn of DDGS ranged from 1,779 to 3,071 kcal/kg and averaged 2,517 kcal/kg. Coefficient of variation for non-amino acid crude protein, ether extract, crude fiber and TMEn were 55.0, 15.7, 15.9 and 17.1%, respectively. The average true amino acid digestibility was 77.32%. Stepwise regression analysis obtained the following equation: TMEn, kcal/kg = -2,995.6+0.88${\times}$gross energy+$49.63{\times}a^*$ (BIC = 248.8; RMSE = 190.8; p<0.01). Removing gross energy from the model obtained the following equation: TMEn, kcal/kg = 57.88${\times}$ether extracts+$87.62{\times}a^*$ (BIC = 254.3, RMSE = 223.5; p<0.01). No correlation was found between color scores and lysine true digestibility (p>0.05). These results suggest that corn DDGS produced in China has a large variation in chemical composition, and gross energy and $a^*$ value can be used to generate TMEn predict equation.

• Asian-Australasian Journal of Animal Sciences
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• 제31권4호
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• pp.564-568
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• 2018

Objective: This experiment was conducted to determine the effects of dietary ${\beta}$-mannanase on the additivity of true metabolizable energy (TME) and nitrogen-corrected true metabolizable energy ($TME_n$) for broiler diets. Methods: A total of 144 21-day-old broilers were randomly allotted to 12 dietary treatments with 6 replicates. Five treatments consisted of 5 ingredients of corn, wheat, soybean meal, corn distillers dried grains with solubles, or corn gluten meal. One mixed diet containing 200 g/kg of those 5 ingredients also was prepared. Additional 6 treatments were prepared by mixing 0.5 g/kg dietary ${\beta}$-mannanase with those 5 ingredients and the mixed diet. Based on a precision-fed chicken assay, TME and $TME_n$ values for 5 ingredients and the mixed diet as affected by dietary ${\beta}$-mannanase were determined. Results: Results indicated that when ${\beta}$-mannanase was not added to the diet, measured TME and $TME_n$ values for the diet did not differ from the predicted values for the diet, which validated the additivity. However, for the diet containing ${\beta}$-mannanase, measured $TME_n$ value was greater (p<0.05) than predicted $TME_n$ value, indicating that the additivity was not validated. Conclusion: In conclusion, the additivity of energy values for the mixed diet may not be guaranteed if the diet contains ${\beta}$-mannanase.

• Journal of Nutrition and Health
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• 제25권7호
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• pp.543-554
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• 1992

True metabolizable energy(TME) is believed a better indicator for animal performance than apparent metabolizable energy (AME) for excluding the endogenous energy losses from excreta, However few researches have been conducted to compare superiority of any energy systems through practical animal feeding tests. Present study was to compare the energy systems in young chicks in terms of predictability of energy intake for the birds performances including body energy retention and of methodological accuracy by evaluating reproducibility and additi-vity of energy values of feed ingredients and compound diets. Five ingredients such as yellow corn wheat soybean meal fish meal and wheat bran were measured for their various biological energy values. in the first feeding trial chicks were restric-ted-fed the basal diet at 80, 60 and 40% on weight basis of the amount of feed ingested by chicks fed ad libitum the same diet. chicks in the second trial were also restricted-fed diets at levels of 80, 70, 60 and 50% on energy basis of the amount consumed by the basak duet group fed ad libitum The diets in the latter trial were however composed of differeent formulations from the basal diet. One-week-old Single Comb White Leghorn male chicks were individually alloted in a cage on 10 cages/treatment basis and fed the diets for 14 days. Individual carcass energy was measured after the feeding trials. Coefficients of variation of energy measurements were lesser for nitrogen-corrected AME and TME(AMEn & TMEn respectively) than AME and TME values suggesting taht reprodu-cibility of energy determinations by former systems could be better than the latters. The coeffi-cients for AME and TME were almkost of the same values. Additivity obtained by the rations between the calculated values and catual measurements appeared quite satisfactory for all the energy systems. Those of AME and TME however were relatively better than the other systems. Regression coefficient ${r}^2$ between energy intake by various systems and chick performances appeared higher for TME, AMEn and TMEn than AME implying that the former systems could provide better predictability for body weight gain and energy retention than the AME. The ${r}^2$ values for TME and AMEn particularly for body weight gain were on the average 0.967 and 0.960 respectively. In conclusion TME or AMEn can be recommended as choice for dietary energy system in terms of performance predictability of the birds and of procedural convenience for the measurements.

• 한국가금학회지
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• 제31권4호
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• pp.255-263
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• 2004

본 연구는 호마박의 사료적 가치를 평가하고 산란계의 성적에 미치는 호마박의 첨가 효과를 구명하기 위해 수행하였다. 실험 1에서는 16수의 ISA-Brown 종계 수탉을 공시하여 강제 급여 방법에 의해 호마박의 진정 대사에너지(TME),질소 보정 진정 대사 에너지 (TMEn)및 진정 아미노산 이용률(TAAA)을 조사하였다. 호마박의 TME 및 TMEn은 각각 2.30 kca1/g및 1.99 kcal/g이었고, 15개 아미노산의 평균 이용률은 $76.93\%$로 나타났다. 실험 2에서는 48주령의 ISA-Brown산란계 90수를 3개 처리(대조구, 호마박 $5\%$, 호마박 $10\%$)로 나누어 4주간의 사양 실험을 실시하였다. 실험사료 내 호마박 첨가가 난 생산성 및 계란 품질에 미치는 영향을 조사하였다. 산란계 사료 내에 호마박을 $5\%$및 $10\%$첨가했을 때 난 생산성 및 생산된 계란의 품질에는 큰 영향을 미치지 않은 것으로 나타났다. 호마박 $10\%$ 첨가구에서 난황 내 Cl8:3 $\omega$3의 비율이 가장 높았으나, 통계적인 유의차는 인정되지 않았다. 혈청 내GOT, G-GT, BUN및 cholesterol 조성에 있어서도 처리간에 큰 차이는 발견되지 않았다. 본 연구에서는 호마박을 정확한 원료 평가를 거쳐 사용한다면 산란계 사료에 있어서 $10\%$까지 사용하여도 난 생산성 및 계란 품질을 저하시키지 않았으며, 공시계에게 어떠한 부정적인 영향도 미치는 않았다는 결과가 시사되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제18권12호
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• pp.1761-1768
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• 2005

Effects of alpha-galactosidase (GAL) on broiler corn-soybean meal diet was investigated. In experiment 1, sixty cockerels were allocated to five groups, including three enzyme treatments (GAL added at 0, 500, and 1,000 mg/kg diet), a nitrogen-free diet group and a fast group. The true nitrogen-corrected ME (TME$_n$) and true amino acid availability were determined. In experiment 2, 324 day-old chicks were used in a 2${\times}$3 factorial design consisting of two energy contents (high and low) and three GAL levels (0, 250, and 500 mg/kg). Three feeding phases, comprising 0-21 d, 22-35 d and 36-48 d, were involved. GAL addition improved TME$_n$ and the availability of methionine and cystine (p<0.05). The apparent ME (AME) or nitrogen-corrected AME (AME$_n$) and digestibility of dry matter, organic matter, calcium, and phosphorus were improved significantly on d 21, so was crude protein and an interaction of energy and GAL on AME$_n$ (p<0.05) was found on d 35. However, daily intake and daily gain were significantly improved with GAL addition (p<0.05) during 21 d. The small intestine relative weight decreased at 250 mg/kg GAL (p<0.05) on d 35, whereas presented an interaction between GAL and energy on d 21 (p<0.05). Likewise, this treatment increased breast muscle ratio (p<0.05). On d 21, triglycerides level of broilers showed interaction between energy and enzyme levels (p<0.05). Uric acid level in 500 mg/kg GAL declined linearly (p<0.05). On d 35, quadratic effects (p<0.05) were observed in total protein, albumin, globulin and cholesterol content for enzyme supplementation. And the interactive effects of energy and GAL on serum values showed more obviously. The study implies that GAL improved energy and nutrient availability of corn-soybean meal diet in broiler. The GAL supplementation to corn-soybean meal based diet can improve performance of broilers in early stages of growth.

• 한국가금학회지
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• 제17권2호
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• pp.101-107
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• 1990

본시험은 Canola 전지종실과 Canola 박 및 Canola 유의 유효에너지가(AM 및 TME)와 아미노산 이용을(TAAA)를 측정하기 위하여 실시하였다. 본시험에는 단관백색레그혼종(S.C.W.C.) 성계수탉이 실시되었으며, AME시험에서는 측정사료인Canola 전지증실, Canola 박 Canola 박＋유(60:40)을 30% 그리고 Canola 유를 10% 배합한 4종의 시험사료와 기초사료를 각각 자유채식시켰고 예비시험기간은 3일 채분기간은 4일이었다. TME와 TAAA시험에서는 Canola 전지종실과 Canola 박은 단-사료로, Canola 박＋유와 Canola 유는 각각 기초사료에 30%와 10%의 비율로 혼합하여 각각 1일 30g을 24시간의 절식기간 후에 강제투여(force feeding)하였고 48시간동안 전분을 채취하였다. 모든 에너지가는 질소정정 에너지가로 환산하였는데, Canola 전지종실, Canola 박, Canola 유 및 Canola ＋유의 AMEn가는 각각 4,485, 1,984. 8,275 및 5,655 kcal/kg이었고 TMEn가는 각각 4,577, 2,103, 8,487 및 5,630kcal/kg으로 측정되어 Canola 박＋유는 Canola 전지종실보다 높은 에너지가를 나타냈으며(P＜0.01), 전반적으로 Canola전지종실은 Canola 박보다 높은 TAAA가를 나타냈다.

• Asian-Australasian Journal of Animal Sciences
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• 제26권4호
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• pp.537-544
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• 2013

This study examined whether pre-treating palm kernel expeller (PKE) with exogenous enzyme would degrade its fiber content; thus improving its metabolizable energy (ME), growth performance, villus height and digesta viscosity in broiler chickens fed diets containing PKE. Our results showed that enzyme treatment decreased (p<0.05) hemicellulose and cellulose contents of PKE by 26.26 and 32.62%, respectively; and improved true ME (TME) and its nitrogen corrected value ($TME_n$) by 38% and 33%, respectively, compared to the raw sample. Average daily gain (ADG), feed intake and feed conversion ratio (FCR) of chickens fed on different dietary treatments in the grower period were not significantly different. Although there was no difference in feed intake (p>0.05) among treatment groups in the finisher period, ADG of chickens in the control (PKE-free diet) was higher (p<0.05) than in all treatment groups fed either 20 or 30% PKE, irrespective of with or without enzyme treatment. However, ADG of birds fed with 20% PKE was higher than those fed with 30% PKE. The FCR of chickens in the control was the lowest (2.20) but not significantly different from those fed 20% PKE diets while birds in the 30% PKE diets recorded higher (p>0.05) FCR. The intestinal villus height and crypt depth (duodenum, jejunum and ileum) were not different (p>0.05) among treatments except for duodenal crypt depth. The villus height and crypt depth of birds in enzyme treated PKE diets were higher (p<0.05) than those in the raw PKE groups. Viscosity of the intestinal digesta was not different (p>0.05) among treatments. Results of this study suggest that exogenous enzyme is effective in hydrolyzing the fiber (hemicellulose and cellulose) component and improved the ME values of PKE, however, the above positive effects were not reflected in the growth performance in broiler chickens fed the enzyme treated PKE compared to those received raw PKE. The results suggest that PKE can be included up to 5% in the grower diet and 20% in the finisher diet without any significant negative effect on FCR in broiler chickens.

• Asian-Australasian Journal of Animal Sciences
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• 제21권7호
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• pp.1053-1058
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• 2008

A total of 392 twenty eight week-old laying hens was used to study the effects of dietary inclusion of solvent-extracted palm kernel cake (PKC) (0%, 12.5% and 25%) and enzyme (mixture of mannanase, ${\alpha}$-galactosidase and protease) supplementation (0 kg/t, 1 kg/t and 2 kg/t) on the performance of laying hens. The levels of PKC did not significantly influence nitrogen corrected true metabolizable energy (TMEn) of the diets. Enzyme-supplemented PKC had significantly higher AME and TMEn values than PKC diets with no enzyme supplementation. Dietary inclusion of 12.5% and 25% PKC in the diets of laying hens did not adversely affect mean egg production or daily egg mass. However, layers consumed significantly more PKC-based diets and had significantly poorer feed conversion ratios (FCR) than controls. However, the feed intake and FCR of hens provided the 12.5% PKC-based diets with enzyme supplementation at 1 kg/t did not differ from the controls. Dietary inclusion of PKC or enzyme did not affect eggshell quality, but egg yolk colour was significantly paler when layers were fed the 25% PKC diet.

• 한국가금학회지
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• 제31권3호
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• pp.145-150
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• 2004

본 실험은 생미강을 부존 사료 자원으로 사용하기 위한 사료적 가치 평가와 육계의 생산성에 미치는 영향을 구명함과 동시에 TAAA와 TMEn을 이용한 사료배합의 필요성을 검증하기 위하여 실시하였다. 실험 1에서는 종계 수탉을 공시하여 생미강을 강제 급여함으로서 TME, TMEn 그리고 TAAA를 측정하였고, 실험 2에서는 대조구, 생미강(5, 10, 15%) 첨가구의 4개 처리구에 처리당 3반복 반복당 6수로 완전 임의 배치하여 사양실험을 실시하였다. 생미강의 TME와 TMEn은 건물기준으로 각각 3.25 kcal/g와 3.12 kcal/g이었고, 16개 아미노산의 평균 생체 이용율(TAAA)은 76.21% 이었다. 3주령부터 6주령까지 실시한 사양실험에서 생미강 첨가구에서 사료섭취량이 다소 증가하는 경향을 나타내었지만, 유의한 차이는 인정되지 않았다. 증체량과 사료요구율에 있어서도 생미강 첨가구에서 다소 높은 경향을 보였으나 유의한 차이는 인정되지 않았다. 본 실험 결과 TMEn과 TAAA를 기초로 사료를 배합할 경우 부존원료의 이용에 의한 성장성적의 저하를 막을 수 있었고, 생미강을 15%까지 첨가하여도 아무런 부정적인 영향 없이 사료를 배합할 수 있어 부존 사료 자원으로 이용할 수 있음이 입증되었다.

• Journal of Animal Science and Technology
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• 제45권5호
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• pp.759-766
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• 2003

본 연구는 탈지미강의 부존사료원료로써의 영양적 가치 평가와 사료 내에 첨가수준을 달리했을 때 육계의 생산성에 미치는 영향을 구명하기 위한 목적으로 수행되었다. 실험Ⅰ에서는 절식구 8수, 탈지미강 급여구 8수, 총 16수의 종계 수탉을 공시하여 강제급여에 의해 탈지미강의 TME, TMEn 및 TAAA를 평가하였다. 탈지미강의 TME와 TMEn은 건물 기준으로 각각 2.19 kcal/g, 2.05 kcal/g이었으며, 15개 아미노산의 평균 생체이용률은 77.29%로 나타났다. 실험Ⅱ에서는 옥수수와 대두박 위주의 대조구 사료와 탈지미강을 5%, 10% 및 15% 수준으로 첨가한 실험사료를 총 72수의 육계 (4처리, 3반복, 반복당 6수)에 3주간 급여하였다. 사료섭취량은 탈지미강 15% 첨가구에서 가장 높았지만 처리간에 통계적인 유의차는 없었다. 주당 증체량은 탈지미강을 여러 수준으로 첨가한 처리구에 비해 대조구에서 가장 높은 것으로 나타났다. 탈지미강 10% 및 15% 첨가구에서는 대조구에 비해 약간 높아졌으나 통계적인 유의차는 인정되지 않았다. 탈지미강 5% 첨가구에서 통계적인 유의차는 나타나지 않았지만 사료요구율이 가장 낮은 것으로 나타났다. 본 실험에서는 사료 원료의 정확한 품질 평가 및 생체이용률을 근거로 사료를 배합했을 때 항영양인자가 함유되어 있지 않는 한 사료의 품질저하를 막을 수 있었고, 탈지미강 역시 가금 사료 내에 다양한 수준으로 첨가하여 부존사료원료로써 이용할 수 있다는 결과가 시사되었다.