• Applied Chemistry for Engineering
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• v.5 no.1
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• pp.121-126
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• 1994

To elucidate the effect on the reactive extraction of acetic acid, various carriers and modifiers were investigated. Carriers used were secondary and tertiary amines and solvation extractant. Diluent was n-butylacetate. Modifiers were 4-nonylphenol, TBP(Tti-n-butyl phospate) and isodecanol. Besides the effect of temperature and pH in aqueous phase were studied. The mixture of 50% tri-n-octyl/n-decylamine tertiary amine, gave higher degree of extraction and selectivity than other extractants in the extraction of acetic acid. It was found that 4-nonylphenol as modifier fairly good. The degree of extraction was higher with decreasing the pH in aquous phase and the temperature of extraction system.

• Environmental Analysis Health and Toxicology
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• v.17 no.1
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• pp.7-11
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• 2002

Effects of nonylphenol (NP) on gonadal development and growth of olive flounder, Paralichthys olivaceus were investigated. NP treatment was carried out to fry fish (during 55 to 64 days after hatching) using oral adminstration at nominal NP concentrations of 50, 100 and 150 tig/g BW Gonad before NP treatment was sexually undifferentiated as observed with mostly gonia cells. At 159 days after hatching, ovarian lamella of ovary were filled with oogonia and perinucleolus oocytes. On the other hand, testicular lobules of the testis were occupied by spermatogonia, spermatocytes and spermatids. Histological observation of ovary and testis development was any different between the control and NP treated groups. However, growth was significantly increased in NP treated groups than control groups (P< 0.05). These results considerate that NP has any effect for sex differentiation and gonadal development, but act for early growth in olive flounder.

• Environmental Analysis Health and Toxicology
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• v.21 no.4 s.55
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• pp.323-330
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• 2006

The aim of current study was to evaluate the toxicity of nonylphenol(NP) on soil nematode, Caenorhabditi elegans. The stress-related gene expression, growth, reproduction and development have been employed to monitor soil toxicity. The 24-h median effect concentrations $(LC_{50s})$ of NP was $0.15mg/L$. The expressions of vitellogenin-6, vitellogenin-2, cytochrome P450 family protein 35a2 and apoptosis enhancer-1 genes were upregulated in C. elegans by NP exposure. Alterations in growth, reproduction and development were also observed in NP-exposed group and especially hatching failure was observed. The overall results indicate that C. elegans has considerable potential as sensitive markers for NP toxicity monitoring.

• Korean Journal of Environmental Biology
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• v.25 no.1
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• pp.66-71
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• 2007

The fourth instar larvae of C. riparius were treated with potential endocrine disruption chemicals (EDCs) such as bisphenol A (BPA) and 4-nonylphenol and the effects of morphological abnormalities were observed. The deformities of the mentum following exposure to EDCs showed the smooth tooth, the loss of tooth and deformed tooth. The incidence rates of the mentum deformity were associated with chemicals: BPA $31{\sim}90%$, and 4-nonlyphenol was $40{\sim}80%$. As the concentration of BPA increased, the incidence of deformed mentum was dose dependent. While the incidences of deformed mentum following exposure to 4-nonlyphenol was not associated with their concentrations. The deformed MLT observed smooth or round tooth and the deformity of LT showed loss of one or more than tooth. Also, the MIX type was usually smooth or loss tooth. The abundance of deformity type for the mentum showed MIX (MLT+LT) > LT (lateral teeth) > MLT (median lateral teeth).

• Analytical Science and Technology
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• v.15 no.2
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• pp.172-179
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• 2002

Determination were performed for the presence of three common environmental endocrine disrupting compounds (EDC), namely nonylphenol (NP), octylphenol (OP), di(2-ethylhexyl)phthalate (DEHP) in rainwater, distilled water, millipore water, groundwater, tap water, and bottle water in market, using OASIS cartrige extraction and GC/MS-SIM. Octylphenol was not detected in any water samples. The order of nonylphenol concentrations were increased in rainwater < millipore water < distilled water < groundwater < tap water < bottle water. The concentration of nonylphenol in bolltle water was $0.44{\mu}g/L$(ppb) at highest. Di(2-ethylhexyl)phthalates were detected in every water samples, and the concentrations were to be $1.70{\sim}2.92{\mu}g/L$ in rainwater, $8.7{\sim}31.7{\mu}g/L$ in distilled water, $0.6{\sim}5.6{\mu}g/L$(mean: $2.5{\pm}0.3{\mu}g/L$) in millipore water, and that were very stable values than other waters, $1.1{\sim}6.0{\mu}g/L$ in groundwater at near Jeonju, $3.1{\sim}5.8{\mu}g/L$ by means in tap water, $0.5{\sim}67.6{\mu}g/L$ in bottle water from market. The concentration of DEHP in bottle water samples from market were in higher than USEPA regulation at over 40%.

• Analytical Science and Technology
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• v.14 no.3
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• pp.244-252
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• 2001

A comprehensive analytical method of endocrine distruptors[i.e., nonylphenol(NP), octylphenol(OP), bis(2-ethylhexyl)phthalate(BEHP)] is fish samples was developed using two internal standards. This method employed closed culture tube extraction with dichloromethane and solvent exchange to iso-hexane and SPE(2g) aminopropyl column, followed by determination on gas chromatograph linked to mass spectrometer(GC/MS) operated in the single ion monitoring(SIM) mode. The recoveries of nonylphenol and octyphenol in the range of $0.2{\sim}20{\mu}g/g$ using 1-phenyl decanol as one internal standard were over 75%, and recovery of bis(2-ethylhexyl)phthalate in the range of $0.4{\sim}40{\mu}g/g$ using bis(2-ethylbutyl)phthalate(BEBP) as the other internal standards was showed over 102%. The present method was applied to fish samples from Korea and UK. The range of concentrations for nonylphnol(NP) and bis(2-ethylhexyl)phthalate in Korean fish were $0.02{\sim}0.06{\mu}g/g$ in 2 samples and $0.18{\sim}2.03{\mu}g/g$ in 9 samples respectively, but bis(2-ethylhexyl)phthalate(BEHP) in UK samples was found $2.99{\mu}g/g$ in just 1 sample. But octylphenol(OP) was not dected in any samples by this method. This two internal standard method provides a more precise analytical tool to investigate endocrine disruptors in a biological matrices of limited quantity.

• Journal of Environmental Science International
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• v.13 no.6
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• pp.561-570
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• 2004

This study was carried out to survey the pollution of nonylphenol (NP) in surface sediments around Gwangyang bay and Yeosu sound. NP was suspected chemicals as endocrine disruption. Gwangyang bay is located on the mid south coast of Korea. It is a semi-closed bay which Yeosu petrochemical industrial complex, POSCO (Pohang Steel Company) and Gwangyang container harbor are there. The surface sediments were collected at 15 stations with gravity corer at October, 1999, February, May and August, 2000. Also, the stream and intertidal sediment were collected at 5 sites at August, 2000. Concentrations of NP in surface sediments were in the range of 6.89 to 202.70 ng/g dry wt.. Seasonal range (mean value) of NP is 13.98 to 30.48 (23.46) ng/g dry wt. at October, 10.35 to 54.91 (28.10) ng/g dry wt. at February, 29.05 to 202.70 (82.32) ng/g dry wt. at May and 6.98 to 83.40 (25.37) ng/g dry wt. at August. NP was seasonally fluctuated, and the highest mean value and range was detected at May, 2000. NP was highly distributed in the inner part of Gwangyang bay than Yeosu sound. Concentrations of NP in stream and intertidal sediments showed the highest value in downstream near Yeosu petrochemical industrial complex and Yondung stream. It suggests that the source of NP is industrial wastewater and municipal sewage.

• Toxicological Research
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• v.17 no.1
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• pp.65-71
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• 2001

The key targets of endocrine disruptors are nuclear hormone receptors, which bind to steroid hormones and regulate their gene transcription. A yeast-based steroid hormone receptor gene trascription assay was previously developed for the evaluation of chemicals with endocrine modulating activity. The yeast transformants used in this assay contain the human estrogen receptor along with the appropriate steroid response elements upstream of the $\beta$-galactosidase reporter gene. We tried to evaluate several natural and synthetic steroids of their potential to interact directly with the steroid receptor. Some putative endocrine disruptors, including nonylphenol, are weakly estrogenic. But the combined treatment oj these chemicals with di-(2-ethylhexyl)phthalate (DEHP) significantly increased the $\beta$-galactosidase activity in the yeast transformant. These results suggest that we also have to consider the synergistic effects of endocrine disruptors. In this study, we showed that yeast-based bioassay is a valuable tool for screening potential endocrine disruptors and quantitative determination of estrogenicity. And the possibility that the estrogen receptor binds multiple environmental chemicals adds another level of complexity to the interaction between the endocrine disruptors and the human hormone system.

• Environmental Analysis Health and Toxicology
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• v.17 no.1
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• pp.1-6
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• 2002

Effects on nonylphenol (NP) treatment on early development of fertilized eggs and survival of larvae in olive flounder, Paralichthys olivaceus were investigated. Fertilized eggs and hatched larvae were exposed to aqueous solutions of NP at nominal concentrations of 50, 100, 150 and 200 $\mu\textrm{g}$/L. In the control I and II (methanol-carrier control) groups, the hatchability of fertilized eggs were 90.7$\pm$7.02 and 90.0$\pm$5.29％ (P <0.05), respectively. However, treatments of NP concentrations at 50, 100, 150 and 200 ug/L were reduced to 78.7$\pm$4.16, 46.0$\pm$9.17, 48.0$\pm$3.46 and 33.3$\pm$11.02% (P<0.05), respectively. The time to hatching of fertilized eggs was delayed in high-dose NP treatment groups rather than control groups. Fertilized eggs of NP immersed group could not hatch normally, and also vertebra of the larvae observed as abnormal shape. Cumulative mortality of hatched larvae after 26hr NP treatment was 38.3, 78.3, 88.3 and 100% in NP 50, 100, 150 and 200 ug/L treatment groups, respectively. Whereas, control I and II were 11.7, 16.7% (P<0.05). Hatched larvae showed most death at the embryonic development stage with abnormalities. These results suggest that NP treatment in fertilized eggs and larvae was inhibited as normally development for fertilized egg and growing of larvae.

• Environmental Analysis Health and Toxicology
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• v.18 no.4
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• pp.249-254
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• 2003

본 연구는 환경호르몬(endocrine disruptors)으로 분류되었으며, 에스트로젠 화합물의 특성을 지닌 4-Nonylphenol (NP)이 설치류 Pituitary 세포 중 성장호르몬을 분비하는 GH3 세포의 Nitric oxide(NO)을 증가시키는 작용기전을 규명코자 수행되었다 먼저 GH3세포에 NP처리 농도에 따른 NO의 생성을 측정한 결과 NP처리농도 의존적으로 증가시켰다. 이러한 NO의 증가가 genomic action인지를 확인하기 위해 GH3세포의 NO를 증가시키는 효소인 neuronal oxide synthase의 단백질량을 측정한 결과 GH3세포에서 NP에 의한 nNOS의 단백질의 변화는 없었다. 에스트로젠 화합물인 NP가 에스트로젠 리셉터 (ER)와의 관계를 조사하기 위해 ER억제제(ICI 168,780)클 처리한 경우 NP에 의해 증가한 NO가 감소하였다. 또한 유전자 전사억제제인 actinomycin D 및 단백질 발현 억제제인 cycloheximide을 처리한 경우는 NP에 의한 NO 증가억제효과가 없었다. 이러한 결과를 종합해 볼 때 GH3 세포에서 NP는 ER을 매개한 non-genomic action에 의해 NO를 증가키는 것으로 사료된다.