• IMMUNE NETWORK
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• v.9 no.5
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• pp.192-202
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• 2009

Background: Little information is available the role of Nitric Oxide (NO) in host defenses during human tuberculosis (TB) infection. We investigated the modulating factor(s) affecting NO synthase (iNOS) induction in human macrophages. Methods: Both iNOS mRNA and protein that regulate the growth of mycobacteria were determined using reverase transcriptase-polymerase chain reaction and western blot analysis. The upstream signaling pathways were further investigated using iNOS specific inhibitors. Results: Here we show that combined treatment with 1,25-dihydroxyvitamin D3 (1,25-D3) and Interferon (IFN)-${\gamma}$ synergistically enhanced NO synthesis and iNOS expression induced by Mycobacterium tuberculosis (MTB) or by its purified protein derivatives in human monocyte-derived macrophages. Both the nuclear factor-${\kappa}B$ and MEK1-ERK1/2 pathways were indispensable in the induction of iNOS expression, as shown in toll like receptor 2 stimulation. Further, the combined treatment with 1,25-D3 and IFN-${\gamma}$ was more potent than either agent alone in the inhibition of intracellular MTB growth. Notably, this enhanced effect was not explained by increased expression of cathelicidin, a known antimycobacterial effector of 1,25-D3. Conclusion: These data support a key role of NO in host defenses against TB and identify novel modulating factors for iNOS induction in human macrophages.

• BMB Reports
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• v.44 no.4
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• pp.267-272
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• 2011

ZAS3 is a large zinc finger transcription repressor that binds the ${\kappa}B$-motif via two signature domains of ZASN and ZASC. A loss-of-function study showed that lack of ZAS3 protein induced accelerated cell proliferation and tumorigenesis. Conversely, gain-of-function studies showed that ZAS3 repressed $NF{\kappa}B$-activated transcription by competing with $NF{\kappa}B$ for the ${\kappa}B$-motif. Based on these observations, we hypothesize that ZAS3 promotes apoptosis by interrupting anti-apoptotic activity of $NF{\kappa}B$. Here, we present evidence that upon $TNF{\alpha}$ stimulation, ZAS3 inhibits $NF{\kappa}B$-mediated cell survival and promotes caspase-mediated apoptosis. The inhibitory effect of ZAS3 on $NF{\kappa}B$ activity is mediated by neither direct association with $NF{\kappa}B$ nor disrupting nuclear localization of $NF{\kappa}B$. Instead, ZAS3 repressed the expression of two key anti-apoptotic genes of $NF{\kappa}B$, TRAF1 and TRAF2, thereby sensitizing cells to $TNF{\alpha}$-induced cell death. Taken together, our data suggest that ZAS3 is a tumor suppressor gene and therefore serves as a novel therapeutic target for developing anti-cancer drugs.

• Journal of Astronomy and Space Sciences
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• v.24 no.2
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• pp.155-166
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• 2007

We have designed a high resolution proton and electron telescope for the detection of high energy particles, which constitute a major part of the space environment. The flux of the particles, in the satellite orbits, can vary abruptly according to the position and solar activities. In this study, a conceptual design of the detector, for adapting these variations with a high energy resolution, was made and the performance was estimated. In addition, a parallel processing algorithm was devised and embodied using FPGA for the high speed data processing, capable of detecting high flux without losing energy resolution, on board a satellite.

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2002.05a
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• pp.134-139
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• 2002

Fracture behaviors of pipes with local wall thinning are very important for the integrity of nuclear power plant. However, effects of local wall thinning on strength and fracture behaviors of piping system were not well studied. Acoustic emission(AE) has been widely used in various fields because of its extreme sensitivity, dynamic detection ability and location of growing defects. In this study, we investigated failure modes of locally wall thinned pipes and AE signals by bending test. From test results, we could be divided four types of failure modes of ovalization, crack initiation after ovalization, local buckling and crack initiation after local buckling. And fracture behaviors such as elastic region, yielding range, plastic deformation range and crack progress could be evaluated by AE counts, accumulative counts and time-frequency analysis during bending test. It is expected to be basic data that can protect a risk according to local wall thinning of pipes, as a real time test of AE.

• Journal of Environmental Science International
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• v.25 no.8
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• pp.1107-1114
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• 2016

This research, sponsored by the Korean Ministry of Environment in 2014, was the first epidemiological study in Korea that investigated the health impact assessment of radon exposure. Its purpose was to construct a model that calculated the annual mean cumulative radon exposure concentrations, so that reliable conclusions could be drawn from environment-control group research. Radon causes chronic lung cancer. Therefore, the long-term measurement of radon exposure concentration, over one year, is needed in order to develop a health impact assessment for radon. Hence, based on the seasonal correction model suggested by Pinel et al.(1995), a predictive model of annual mean radon concentration was developed using the year-long seasonal measurement data from the National Institute of Environmental Research, the Korea Institute of Nuclear Safety, the Hanyang University Outdoor Radon Concentration Observatory, and the results from a 3-month (one season) survey, which is the official test method for radon measurement designated by the Korean Ministry of Environment. In addition, a model for evaluating the effective annual dose for radon was developed, using dosimetric methods. The model took into account the predictive model for annual mean radon concentrations and the activity characteristics of the residents.

• Journal of the Korean earth science society
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• v.31 no.7
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• pp.673-680
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• 2010

A steel pile often utilized to bear heavy loads of the upper sub-structure, e. g., bridge constructions and tall buildings. As the steel piles are driven in the underground, it is not easy to detect the depth of the existing pile foundation when there is no detailed foundation information available. However, accurate informations of the depths of piles becomes critical required when reinforcing the existing structures or constructing new ones at the adjacent stage to assure the safety of existing structures. In this study, we tested the applicability of the three components borehole fluxgate magnetometer for detecting the depths and locations of steel piles which are commonly used in civil engineering. Results showed that the information of location as well as the depth of steel piles could be obtained by using data from the three components borehole fluxgate magnetometer.

• Journal of the Korea Institute of Military Science and Technology
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• v.18 no.5
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• pp.582-593
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• 2015

For military training and course-of-action analysis, OneSAF Int'l version being used in ROK Army has a limited capability to simulate NBC(nuclear, biological, and chemical) damages. For high-fidelity NBC combat simulation, it is required to visualize NBC contamination dispersion in consideration of weather conditions and terrain characteristics. However, OneSAF itself handling interaction among thousands of combat entities cannot carry out a simulation of NBC contamination dispersion because it brings about an excess burden. To resolve this problem, this research aims to design simulation federation for analysis on NBC operational effects. After examining design consideration to connect OneSAF and a NBC contamination dispersion model, we design a federation architecture that facilitates the interaction between OneSAF and a NBC contamination dispersion model. Afterwards, we implement a federation interface to share simulation data by publish-subscribe pattern and to translate them into the proprietary format for each model. We prove the possibility of federation between both models, as showing that dispersion of NBC contaminated cloud and changes in concentration are reflected in OneSAF-based engagement simulation.

• BMB Reports
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• v.30 no.6
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• pp.431-437
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• 1997

Since the role of CD40 on the interleukin-4(IL-4) -induced B cell activation has been strongly implicated in the agumentation of IgE production and response, we have investigated the intracelluar signaling pathways utilized by IL-4 and CD40 for type II IgE receptor (CD23) expression. IL-4 and anti-CD40 antibody treatment of human B cells, independently caused a rapid induction of CD23 gene activation within 2 h. There was a noticeable synergism between the action of the two agents inducing CD23 expression: the addition of anti-CD40 to the IL-4-treated culture significantly agumented the IL-4-induced CD23 on both mRNA and surface protein levels, and the inclusion of IL-4 in the anti-CD40-treated cells caused a further increase of CD23 expression far above the maximal level induced by anti-CD40. Protein tyrosine kinase (PTK) inhibitors effectively suppressed the both IL-4- and anti -CD40-induced CD23 expression. whereas protein kinase C (PKC) inhibitors had no effects. Electrophoretic mobility shift assays (EMSA) have shown that IL-4 and anti-CD40 induce the activation of NF-IL-4 and $NF-_{K}B$, respectively, binding to the CD23 promoter, both in a PKC-independent and PTK-dependent manner. These data suggest that the synergistic activation of CD23 gene expression by IL-4 and anti-CD40 is mediated by co-operative action of distinct nuclear factors. each of which is rapidly activated via PKC-independent and PTK-dependent process.

• BMB Reports
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• v.51 no.7
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• pp.356-361
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• 2018

Actin-binding LIM protein 1 (ABLIM1), a member of the LIM-domain protein family, mediates interactions between actin filaments and cytoplasmic targets. However, the role of ABLIM1 in osteoclast and bone metabolism has not been reported. In the present study, we investigated the role of ABLIM1 in the receptor activator of $NF-{\kappa}B$ ligand (RANKL)-mediated osteoclastogenesis. ABLIM1 expression was induced by RANKL treatment and knockdown of ABLIM1 by retrovirus infection containing Ablim1-specific short hairpin RNA (shAblim1) decreased mature osteoclast formation and bone resorption activity in a RANKL-dose dependent manner. Coincident with the downregulated expression of osteoclast differentiation marker genes, the expression levels of c-Fos and the nuclear factor of activated T-cells cytoplasmic 1 (NFATc1), critical transcription factors of osteoclastogenesis, were also decreased in shAblim1-infected osteoclasts during RANKL-mediated osteoclast differentiation. In addition, the motility of preosteoclast was reduced by ABLIM1 knockdown via modulation of the phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K)/Akt/Rac1 signaling pathway, suggesting another regulatory mechanism of ABLIM1 in osteoclast formation. These data demonstrated that ABLIM1 is a positive regulator of RANKL-mediated osteoclast formation via the modulation of the differentiation and PI3K/Akt/Rac1-dependent motility.

• Proceedings of the Korean Society of Medical Physics Conference
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• 2004.11a
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• pp.39-42
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• 2004

The Central Electrode Correction Factor that corrects the effect due to the electrode being made of non-air material is base on the data from the depth of 5cm in water. But TG-51 protocol proposes the reference depth of 10cm in water. The purpose of this research is to check the alteration of Central Electrode Correction Factor due to the change of reference depth from 5cm to 10cm in water using Monte Carlo Computing Methods. The results showed that the change of Central Electrode Correction Factor is ignorable in the statistical errors of 2% for two different depth, 5cm and 10cm.