• Title/Summary/Keyword: OLR1

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Cloning of OLR1 Gene in Pig Adipose Tissue and Preliminary Study on Its Lipid-accumulating Effect

  • Sun, Chao;Liu, Chun-wei;Zhang, Zhong-pin
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.10
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    • pp.1420-1428
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    • 2009
  • In this study we cloned and characterized a novel lipid-accumulating gene, the oxidized low-density lipoprotein receptor 1 (OLR1), which is associated with lipogenesis. We analyzed the gene structure and detected the mRNA transcriptional expression levels in pig adipose tissues at different months of age (MA) and in different economic types (lean type and obese type) using real-time fluorescence quantitative PCR. OLR1 expression profile in different tissues of pig was analyzed. Finally, we studied the correlation between OLR1 and lipid metabolism related genes including peroxisome proliferator-activated $receptor{\gamma}2$ ($PPAR{\gamma}2$), fatty acid synthetase (FAS), triacylglycerol hydrolase (TGH), CAAT/enhancer binding protein $\alpha$ ($C/EBP{\alpha}$) and sterol regulatory element binding protein-1c (SREBP-1c). Results indicated that the OLR1 gene of the pig exhibited the highest homology with the cattle (84%), and the lowest with the mouse (27%). The signal peptide located from amino acid 38 to 60 and the domain from amino acid 144 to 256 were shared by the C-type lectin family. The expression level of OLR1 in pig lung was exceedingly higher than other tested tissues (p<0.01). In pig adipose tissue, the expression level of OLR1 mRNA increased significantly with growth (p<0.01). The expression level of OLR1 mRNA in obese-type pigs was significantly higher than that of lean-type pigs of the same monthly age (p<0.05). In adipose tissue, the expression of OLR1 correlated with $PPAR{\gamma}2$, FAS and SREBP-1c, but not TGH or C/EBP${\alpha}$. In conclusion, OLR1 was highly associated with fat deposition and its transcription, as suggested by high correlations, was possibly regulated by $PPAR{\gamma}2$ and SREBP-1c.

A Sensitivity Analysis of Accuracy for COMS Outgoing Longwave Radiation Product

  • Kim, Hyunji;Han, Kyung-Soo;Lee, Chang Suk;Shin, Inchul
    • Korean Journal of Remote Sensing
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    • v.31 no.1
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    • pp.39-46
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    • 2015
  • Outgoing Longwave Radiation (OLR) is emitted energy from the Earth that is an important indicator of cooling effect in global scale and meteorological events in regional scale. Satellite-driven OLR products have its advantages overcoming spatially limited representation. The Korean geostationary satellite, Communication, Ocean and Meteorological Satellite (COMS), has been producing OLR product in accordance with its own algorithm since Apr. 2011. This study introduces Spatio-Temporally Equalized Match-up (STEM) approach to evaluate the COMS OLR products. We have tested a number of cases of thresholds set by standard deviations of a subpixel $10.8{\mu}m$ to find optimal representation of OLR in the selective match-up. Each case was then validated with broadband reference data, Clouds and the Earth's Radiant Energy System (CERES). We found that selective STEM approach was useful to validate OLR product especially its distribution in homogeneous grids.

Slaughterhouse wastewater treatment in a bamboo ring anaerobic fixed-bed reactor

  • Tritt, Wolfgang P.;Kang, Ho
    • Environmental Engineering Research
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    • v.23 no.1
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    • pp.70-75
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    • 2018
  • A pilot scale anaerobic fixed-bed with a reactor volume of $2.8m^3$ was built to treat slaughterhouse wastewater. Bamboo rings were chosen as support media mainly because of their cheaper price in underdeveloped countries. Even with their lower porosity and specific surface, the reactor performance showed a maximum 95% COD removal efficiency at an organic loading rate (OLR) of $1kg\;COD/m^3-d$ with its corresponding hydraulic retention time (HRT) of 7.5 d. At a higher OLR of $4.0kg\;COD/m^3-d$, the COD removal efficiency of 75% was achieved with an HRT of 2 d. No big difference in COD removal efficiencies was found between the reactors operated in both upflow and downflow modes. Their operational behavior and effluent characteristics were similar. The effluent COD/TKN ratio of 6.67 at an OLR of $4.0kg\;COD/m^3-d$ was only marginal acceptable range for a subsequent biological denitrification process. Otherwise carbon supplementation is required at a lower OLR.

A Comparison of ERBE and AVHRR Longwave Flux Estimates (ERBE와 AVHRR에 의하여 추정된 지구의 장파복사량 비교)

  • 오성남
    • Korean Journal of Remote Sensing
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    • v.6 no.2
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    • pp.75-88
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    • 1990
  • NOAA 위성의 narrow-band AVHRR(Advanced Very. High Resolution Radiometer) 적 외선 채널과 broad-band 0.2 - 50$\mu\textrm{m}$ 영역의 ERBE(Earth Radiation Budget Experiment) scanning instrument에 의하여 관측된 radiance로부터 추정된 지구의 대기 외장파복사량 (Outgoing Longwave Radiation:OLR) 이 비교조사되었다. 이를 위하여 1985년 4월, 7월, 10월과 1986년 1월에 위성에서 관측된 radiance를 각각 이용하였고 비교된 OLR은 위도와 경도가 각각 2.5$^{\circ}$ 간격으로 구분된 grid내에서 일치(collocate)시켜 지역별(zonal), 그리고 전지구(global)규모 로 비교되었다. ERBE와 AVHRR에 의하여 각각 추정된 OLR값의 차(ERBE minus AVHRR)에 의한 분석 결과는 주간의 경우 -1~2 W/m$^2$의 값과 야간의 경우 4~7 W/m$^2$의 값으로 비교적 좋은 일치를 보였지만 이들의 RMS는 하절(6월)에 12 W/m$^2$와 동절(12월)에 5 W/m$^2$의 값으로 다소 높은 차이를 보였다. 한편, 이들 OLR값을 관측지역에 따라 큰 차 이를 나타내어 사막지역과 아열대고기압(subtropical ocean)대에서는 상반된 결과를 보였다. 이들 지역에 대한 차이는 지역적 기온구조와 지표온도의 영향을 다 고려하지 못하고 OLR 측정치를 도 출하는 대기복사모델(radiation model)의 regional systematic bias에서 기인된 것으로 해석된다. 즉 사막의 지표역전층에 대한 AVHRR과 해면의 대기구조에 대한 ERBE의 OLR은 상반된 영향을 보였다.

An Improved Estimation of Outgoing Longwave Radiation Based on Geostationary Satellite

  • Kim, Hyunji;Seo, Minji;Seong, Noh-hun;Lee, Kyeong-sang;Choi, Sungwon;Jin, Donghyun;Huh, Morang;Han, Kyung-Soo
    • Korean Journal of Remote Sensing
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    • v.35 no.1
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    • pp.195-201
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    • 2019
  • The Outgoing Longwave Radiation (OLR) is an important satellite-driven variable for understanding the Earth's energy budget balance. The geostationary OLR retrievals require angular and spectral integration using an empirical equation for irradiance flux-to-OLR from a regression analysis, which determines the accuracy of the narrowband satellite-based OLR. We selected homogeneous pixels which is satisfied less temporal-spatial variability of cloud, on three infrared channels (6.7, 10.8, $12.0{\mu}m$) of the first multipurpose geostationary satellite in Korea, namely the Communication, Ocean and Meteorological Satellite/Meteorological Imager (COMS/MI). Multiple regression analysis was performed to retrieve OLR with improved accuracy using selected parameters based on theoretical and physical significance. This algorithm yielded retrieval with higher accuracy than broadband-based OLR retrieval: RMSE of 10.54 to $3.81W\;m^{-2}$, and bias of -8.49 to $-0.07W\;m^{-2}$.

Performance Evaluation of Anaerobic Bioreactors and Effects of Ammonia on Anaerobic Digestion in Treating Swine Wastewaters

  • Lee, Gook-Hee;Seo, Jun-Won;Kim, Jong-Soo
    • Korean Journal of Environmental Agriculture
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    • v.25 no.3
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    • pp.195-201
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    • 2006
  • The operational characteristics of anaerobic bioreactors in treating swine wastewater were evaluated upto hydraulic retention time (HRT) of 1 day and organic loading rate (OLR) of $5.1kg-COD/m^3{\cdot}d$ for 200 days. The bioreactors were effective in treating swine wastewaters with COD removal efficiency of $78.9{\sim}81.5%$ and biogas generation of $0.39{\sim}0.59m^3/kg-COD_r$ at OLR of $1.1{\sim}2.2kg-COD/m^3{\cdot}d$. The two-stage ASBF anaerobic bioreactors was effective in treating different characteristics of swine wastewaters since they showed high and stable COD removal efficiency at high OLR due to effective retention of biomass by media and staging. The effects of ammonia on anaerobic digestion were investigated by operating two-stage ASBF reactors using swine wastewaters as influent without and with ammonia removal at HRT of $1{\sim}2$ days and OLR of $2.2{\sim}9.6kg-COD/m^3{\cdot}d$ for 250 days. The COD removal efficiency and biogas generation of two-stage ASBF reactors was decreased by increasing influent ammonia concentrations to 1,580 mg (T-N)/L with increasing OLR to $6.3kg-COD/m^3{\cdot}d$, while those were increased by maintaining influent ammonia concentrations below 340 mg (T-N)/L by MAP precipitation with increasing OLR to $9.6kg-COD/m^3{\cdot}d$. Initial inhibition of ammonia on anaerobic processes was observed at a concentration of 760 mg (T-N)/L and the COD removal efficiency and biogas generation dropped to 1/2 at ammonia concentration ranges of $1,540{\sim}1,870mg$ (T-N)/L. It is essential to remove ammonia in swine wastewaters to an initial inhibition level before anaerobic processes for the effective removal of COD.

Analysis of the oxidized low density lipoprotein receptor 1 gene as a potential marker for carcass quality traits in Qinchuan cattle

  • Gui, Lin-sheng;Raza, Sayed Haidar Abbas;Jia, Jianlei
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.1
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    • pp.58-62
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    • 2019
  • Objective: The oxidized low density lipoprotein receptor 1 (OLR1) gene plays an important role in the degradation of oxidized low-density lipoprotein and adipocyte proliferation in mammals. For this reason, we aimed at investigating the association of OLR1 gene polymorphisms with carcass quality traits in Chinese Qinchuan cattle. Methods: The single nucleotide polymorphism (SNP) was identified in the 3' untranslated region of bovine OLR1 gene by DNA sequencing. In addition, the haplotype frequency and linkage disequilibrium estimates of three SNPs were evaluated in 520 individuals. Results: Results indicated that the studied three SNPs were within the range of moderate genetic diversity (0.25< polymorphism information content<0.5). Haplotype analysis of three SNPs showed that ten different haplotypes were identified, but only five haplotypes were listed as those with a frequency of <0.05 were excluded. The Hap3 ($-G_1T_2C_3-$) had the highest haplotype frequency (42.10%). Linkage disequilibrium analysis showed that the three SNPs had a low linkage ($r^2<0.001$). The T10588C and C10647T were significantly associated with backfat thickness and intramuscular fat content in Qinchuan cattle. Conclusion: Based on our results, we believe that the OLR1 gene could be a strong candidate gene for influencing carcass quality traits in Qinchuan cattle.

Effects of the Recirculation Port Location on Treatment Efficiency of an Anaerobic Hybrid Reactor Consisted of a Fluidized Bed and a Packed Bed (유동상과 충전상이 결합된 혐기성 혼성 반응조에서 순환수의 인출지점이 처리효율에 미치는 영향)

  • Kim, Seong-Yong;Park, Soo-Young;Cho, Kwang-Myeung
    • Journal of Korean Society of Environmental Engineers
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    • v.22 no.11
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    • pp.1935-1944
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    • 2000
  • This research was performed to investigate the effects of the location of recirculation port on the wastewater treatment efficiency of an anaerobic hybrid reactor consisted of a fluidized bed and a packed bed. The recirculation port was located either at the top of the packed bed (Reactor 2) or above the fluidized bed (Reactor 1). Media for the fluidized bed and the packed bed were granular activated carbon and Pall ring-type plastic media. respectively. At organic loading rates(OLR) up to $6.2kg\;COD/m^3-day$. Reactor 2 showed somewhat better performance than Reactor 1 with COD removal efficiencies of 85.0-95.2%. The COD removal efficiencies of the reactors drastically deteriorated at OLRs above $6.2kg\;COD/m^3-day$, and the tendency was more severe for Reactor 1 than for Reactor 2. Eventhough the two reactors showed similar effluent SS concentrations at OLRs below $3.6kg\;COD/m^3-day$, Reactor 2 showed higher effluent SS concentrations than Reactor 1 at OLRs above $5.3kg\;COD/m^3-day$. Reactor 2 was stabler than Reactor 1 with a methane production rate of $5.5kg\;COD/m^3$-day at the OLR of $13.3kg\;COD/m^3-day$. An abrupt increase in effluent volatile acid concentration was observed at the OLR of $6.2kg\;COD/m^3-day$ for Reactor 1 and $7.1kg\;COD/m^3-day$ for Reactor 2. and the increase was greater in Reactor 1. In conclusion. the range of OLR for adequate treatment in the hybrid reactor was determined according to the location of the internal recirculation port. It is more desirable for higher OLRs to locate the recirculation port at the top of the packed bed in order to utilize the whole volume of the reactor.

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Ammonia Effects on Anaerobic Digestion of Swine Wastewaters (양돈폐수의 혐기성 소화에 대한 암모니아의 영향)

  • Kim, Jong-Soo
    • Journal of the Korea Organic Resources Recycling Association
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    • v.10 no.1
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    • pp.102-108
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    • 2002
  • The effects of ammonia on mesophilic anaerobic digestion were investigated by operating lab-scale two-stage ASBF reactors using swine wastewaters as influent without and with ammonia removal at HRT of 1-2 days and OLR of $2.2{\sim}9.6kg-COD/m^3{\cdot}d$ for 250 days. The COD removal efficiency and biogas generation of two-stage ASBF reactors was decreased by increasing influent ammonia concentrations to 1,580 mg(T-N)/L with increasing OLR to $6.3kg-COD/m^3{\cdot}d$, while those were increased by maintaining influent ammonia concentrations below 340 mg(T-N)/L by MAP precipitation with increasing OLR to $9.6kg-COD/m^3{\cdot}d$. Initial inhibirion of ammonia on anaerobic processes was observed at a concentration of 760 mg(T-N)/L and the COD removal efficiency and biogas generation dropped to 1/2 at ammonia concentration ranges of 1,540~1,870 mg(T-N)/L. It is essential to remove ammonia in swine wastewaters to an initial inhibition level before anaerobic processes for the effective removal of COD.

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Evaluation and Intercomparisons of the Estimated TOVS Precipitable Waters for the Tropical Plume (Tropical Plume 에 대한 TOVS 추정 가강수량의 평가와 상호비교)

  • 정효상;신동인
    • Korean Journal of Remote Sensing
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    • v.9 no.2
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    • pp.51-69
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    • 1993
  • Precipitable Water(PW) are retrieved over the tropical and subtropical Pacific Ocean from TOVS infrared and microwave channel brightness temperature and OLR observations by means of stepwise linear regression. The retrieved TOVS PW fields generated by PW$_{sfc}$(71.1 % of the variance and 0.62 g cm$^{-2}$ standard error over the surface) and PW$_{700500}$(71.7 % and 0.17 g cm$^{-2}$ over the 700 - 500 hPa layer) revealed more evolving synoptic signals over the tropical and subtropical Pacific Ocean. The PW$_{sfc}$ dose not show significantly the TP feature because of the representation of the lower PW for high-level clouds not associated with deep convection. There exists some elusion to trace the TP on the PW$_{sfc}$ field if any supplementary information does not provide. But ECMWF analysis has a general tendency of drying the subtropics and moistening the ITCZ (InterTropical Convergence Zone) and SPCZ(South Pacific Convergence Zone). However, although ECMWF analysis is fairly successful in capturing mean patterms, it is unsuccessful in following active synoptic signal like a tropical plume. Similarly, SMMR-PW does not represent the TP well which consists of the highand middle-level clouds, but PW$_{sfc}$ shows underestimated moistness of TP and does not depict significant signal of TP. In the PW field derived from microwave observations, the TP can not be recognized well. Furthermore, the signature of PW$_{sfc}$ was different from OLR for the TP, which implies the presence of high- and middle-layer thin clouds, but in a closer agreement for deep and active convection areas which contain thick middle- and lower-layer clouds; though OLR represented the cloudiness in the tropics well. In synoptically active regions, it differed from OLR analysis, primarily bacause of actual differences in water vapor and cloud features. The signature of PW$_{sfc}$ was different from OLR for the TP.