• The KIPS Transactions:PartC
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• v.17C no.4
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• pp.335-346
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• 2010

Mac OS X is the Computer Operating System that develop in Apple Inc. Mac OS X is the successor to Mac OS 9 Version which had been Apple's primary operating system since 1984. Recently, Mac OS X 10.6 (Snow Leopard) has been manufactured and is distributed to user. Apple's Mac OS X Operating System is occupying about 10% in the world Operating System market share. But, Forensic tools that is utilized on digital forensic investigation can not forensic analysis about Mac OS X properly. To do forensic investigation about Mac OS X, information connected with user's action and trace can become important digital evidence in Operating System. This paper presents way about user trace data acquisition methodology in Mac OS X.

• Journal of Life Science
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• v.27 no.12
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• pp.1393-1402
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• 2017

In plants, calcium ($Ca^{2+}$)-dependent protein kinases (CDPKs) are important sensors of $Ca^{2+}$ signals. Previous research demonstrated the expression of the OsCPK11 gene in various tissues at the transcription level, but its developmental and biochemical functions at the protein level were not determined. This study was aimed to identify biochemical characteristics of OsCPK11. GST- OsCPK11 was expressed in E. coli and used for an in vitro kinase assay. Biochemical analyses identified OsCPK11 as a CDPK. OsCPK11 autophosphorylated itself and transphosphorylated histone III-s and MBP as substrates in the presence of $Ca^{2+}$. The activity of the recombinant OsCPK11 was influenced by $Mg^{2+}$, with optimum activity detected at pH 7.0-7.5. OsCPK11 activity was not affected by $Mg^{2+}$, $Mn^{2+}$, or $Na^+$ in the presence of a high level of $Ca^{2+}$. Autophosphorylation of OsCPK11 decreased $Ca^{2+}$ sensitivity of OsCPK11. An anti-OsCPK11 rabbit antibody recognized 95.5 kD of GST-OsCPK11, as shown by an immunoblot analysis. These results shed light on the function of OsCPK11 in $Ca^{2+}$-mediated signaling in rice.

• Molecules and Cells
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• v.37 no.7
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• pp.532-539
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• 2014

We isolated a rice (Oryza sativa L.) WRKY gene which is highly upregulated in senescent leaves, denoted OsWRKY42. Analysis of OsWRKY42-GFP expression and its effects on transcriptional activation in maize protoplasts suggested that the OsWRKY42 protein functions as a nuclear transcriptional repressor. OsWRKY42-overexpressing (OsWR KY42OX) transgenic rice plants exhibited an early leaf senescence phenotype with accumulation of the reactive oxygen species (ROS) hydrogen peroxide and a reduced chlorophyll content. Expression analysis of ROS producing and scavenging genes revealed that the metallothionein genes clustered on chromosome 12, especially OsMT1d, were strongly repressed in OsWRKY42OX plants. An OsMT1d promoter:LUC construct was found to be repressed by OsWRKY42 overexpression in rice protoplasts. Finally, chromatin immunoprecipitation analysis demonstrated that OsWRKY42 binds to the W-box of the OsMT1d promoter. Our results thus suggest that OsWRKY42 represses OsMT1d-mediated ROS scavenging and thereby promotes leaf senescence in rice.

• KIPS Transactions on Software and Data Engineering
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• v.2 no.3
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• pp.151-160
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• 2013

iOS applications refer to the apps operating on iOS, a mobile OS developed by Apple. As iOS provides graphic user interfaces based on touch screens, most of iOS apps support GUIs. GUIs become increasingly important for iOS apps. So are GUI tests. As GUI functions are performed by event handlers, faulty event handlers could cause defects in GUIs. Hence, this study detects faults in event handlers as a way to test GUIs for iOS apps, and suggests how to generate test cases by re-defining input domains of event handlers.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.30-30
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• 2022

Philippines is the second world supplier of coconut by-products. As its first major genomics project, the Philippine Genome Center program for Agriculture (PGC-Agriculture) took the challenge to sequence and assemble the whole coconut genome. The project aims to provide advance genetics tools for our collaborating coconut researchers while taking the opportunity to initiate local capacity. Combination of different NGS platforms was explored and the Philippine 'Catigan Green Dwarf' (CATD) variety was selected with the breeders to be the crop's reference genome. A high quality genome assembly of CATD was generated and used to characterize important genes of coconut towards the development of resilient and outstanding varieties especially for added high-value traits. The talk will present the significant results of the project as published in various papers including the first report of whole genome sequence of a dwarf coconut variety. Updates will include the challenges hurdled and specific applications such as gene mining for host insect resistance and screening for least damaged coconuts (thus potentially insect resistant varieties). Genome-wide DNA markers as published and genes related to coconut oil qualitative/quantitative traits will also be presented, including initial molecular/biochemical studies that support nutritional and medicinal claims. A web-based genome database is currently built for ease access and wider utility of these genomics tools. Indeed, a major milestone accomplished by the coconut genomics research team, which was facilitated with the all-out government support and strong collaboration among multidisciplinary experts and partnership with advance research institutes.

• Herbal Formula Science
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• v.11 no.1
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• pp.171-195
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• 2003

Objectives: This experimental study has been done to compare the Os Draconis composition before and after using processing method. Os Draconis has a quality for calming the liver meridian function and relaxation the mind. Methods: I studied the Os Draconis and processed Os Draconis by vinegar to compare the compositions and its' character. Results: Os Draconis is not a dinosaur's bone fossil but a mammal's bone fossil which has a Calcite mineral, an Apatite mineral, $SiO_2\;Al_2$ O, etc. Os Draconis contains a main ingredients CaO>50.00%. Processed Os Draconis which is heated and soaked in vinegar changes to weak condition Conclusion: Os Draconis is supposed to be a mammal's bone fossil. Some Os Draconis has a radioactive substance like a U, Th so we pay heed to deal with it in a medical clinic.

• Proceedings of the Korean Information Science Society Conference
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• 2007.10b
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• pp.269-273
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• 2007

하드웨어의 급속한 성능증가와 서버의 동적인 활용을 위하여 가상화 기술의 연구 및 개발이 급격히 증가하고 있다. 이러한 가상화 기술의 성능을 측정하기 위한 기존의 방법들은 가상머신에 설치된 Guest OS 만을 고려하거나, 시스템이 설치된 하드웨어를 대상으로 통합적으로 평가되었다. 하지만, 여러 개의 Guest OS를 사용하는 경우/ Host OS와 Guest OS가 서로 통신할 경우에서는 이와 같은 측정 방법에 문제점이 발생한다. 본 논문은 Host OS와 Guest OS가 서로 통신할 경우 발생하는 Host OS의 자원 사용을 평가하여 Guest OS의 성능에 미치는 영향을 분석하고, 이러한 영향을 정확히 분석하기 위한 방법을 제안한다.

• Proceedings of the Botanical Society of Korea Conference
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• 1985.08b
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• pp.73-81
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• 1985

We have previously isolated OsMADS4 gene that is a member of the class B MADS box genes from rice. In this study, another member of the class B MADS box genes was isolated from rice flower by the yeast two-hybrid screening method using OsMADS4 as bait. RNA blot analyses revealed that the clone, OsMADS16, was expressed in the second and third whorls, whereas the OsMADS4 transcripts were present in the second, third, and fourth whorls. These expression patterns of the OsMADS16 and OsMADS4 genes are very similar with those of AP3 and PI, the class B genes of Arabidopsis, respectively. In the yeast two-hybrid system, OsMADS4 interacted only with OsMADS16 among several rice MADS genes investigated, suggesting that OsMADS4 and OsMADS16 function as a heterodimer in specifying sepal and petal identities. We have also isolated OsMADS6 gene using OsMADS1 as a probe. Both are members of the AGL2 MADS family. Various MADS genes that encode for protein-protein interaction partners of the OsMADS6 protein were isolated by the yeast two-hybrid screening method. A majority of these genes belong to the AGL2 family. Sequence Homology, expression pattern, and ectopic expression phenotypes indicated that one of the interaction partners, OsMADS14, appears to be homologous to API, the class A MADS gene of Arabidopsis.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.149-149
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• 2017

The RING (Really Interesting New Gene) finger proteins are known to play crucial roles in various abiotic stresses in plants. In this study, we report on RING finger E3 ligase, ${\underline{O}ryza}$ ${\underline{s}ativa}$ ${\underline{s}alt$-, ${\underline{A}BA}$- and ${\underline{d}rounght}$ stress-${\underline{i}nduced}$ RING finger ${\underline{p}}rotein{\underline{1}}$ gene (OsSAD1). In vitro ubiquitination assay demonstrated that unlike OsSAD1, a single amino acid substitution ($OsSAD1^{C168A}$) of the RING domain showed no E3 ligase activity, supporting the notion that the activity of most E3s is specified by a RING domain. Result of Yeast-Two hybridization, In vivo protein degradation assay supports that OsSAD1 interacting with 3 substrate, OsSNAC2, OsGRAS44 and OsPIRIN1, and mediates proteolysis of 3 substrates via the 26S proteasome pathway. Subcellular localizations of OsSAD1 while approximately 62% of transient signals were detected in cytosol, 38% of signals were showed nucleus. However, transiently expression of OsSAD1 was detected in cytosol 30% while as 70% of nucleus under 200 mM salt treated rice protoplasts. Results of bimolecular fluorescence complementation (BiFC) showed that two nucleus-localized proteins (OsSNAC2 and OsGRAS44) interacted with OsSAD1 in the both cytosol and nucleus. Heterogeneous overexpression of OsSAD1 Heterogeneous overexpresssion of OsSAD1 in Arabidopsis exhibited sensitive phenotypes with respect to Salt-, mannitol-responsive seed germination, seedling growth. In ABA conditions, OsSAD1 overexpression plants showed highly tolerance phenotypes, such as root length and stomatal closure. Our findings suggest that the OsSAD1 may play a negative regulator in salt stress response by modulating levels of its target proteins.

• Proceedings of the Korean Information Science Society Conference
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• 2001.04a
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• pp.31-33
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• 2001

최근 web기반에서 언제 어디서나 사용자만의 컴퓨터환경을 제공하는 Virtual OS를 구현한 사례가 많이 발표되고 있다. [1, 2, 3]. Virtual OS는 Unix나 Windows같은 Real OS를 기반으로 하여 web에서 OS의 기능을 구현한 것으로 Real OS에 있는 프로그램을 실행하고 파일을 관리한다. 본 논문에서는 Web기반 Virtual OS에서 동작하는 C 언어 Preprocessor를 설계한다. Web기반으로 preprocessor를 설계하면 어디서나 사용자가 프로그래밍을 할 수 있는 환경을 구축할 수 있다. 본 논문에서는 Linux를 기반으로 preprocessor를 구현하였으며, 분석 결과를 시뮬레이션을 통해 알아보았다.