• The Journal of Korean Medicine
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• v.27 no.1 s.65
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• pp.11-22
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• 2006

Objectives : In this study, the aim was to investigate the inhibitory effects of Bangpungtong-seoung-san on the obese gene and obese inhibitory about obese-mouse induced by high fat diet. Methods : In order to investigate the effects of Bangpuangtongseoung-san(BPTS) on the obese gene and obese inhibitory, C57BL/6 mice were induced by high fat diet. C57BL/6 mice were divided into three groups(normal, high fat diet with control, high fat diet with BPTS extract) and fed for 13weeks. Results : 1. The change of body weight and the final increase of body weight were decreased significantly. 2. The amount of the adipocyte in body weight was decreased significantly. 3. In primary adipocytes, ${\beta}3AR$ gene expression was increased significantly and leptin gene expression was decreased in $100{\mu}g/ml$ density significantly. 4. In primary adipocytes, the amount of TNF-${\alpha}$ was decreased in $100{\mu}g/ml$ density significantly and the amount of leptin was decreased but did not show significance. 5. In adipocytes tissue, the expression of leptin was decreased and the expression of ${\beta}3AR$ was increased. Conclusions : These results suggest that BPTS may inhibit the expression of the obese gene mad obese inhibitory about obese-mouse induced by high fat diet.

• The Journal of Korean Medicine
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• v.29 no.2
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• pp.116-132
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• 2008

Objective: This study investigated the effects of Bimanbang-1 (肥滿1號方: here in after referred to BMB-1) on the obese gene and obese inhibitory. Methods: C57BL/6 mice were induced by high fat diet. Mice were divided into three groups (normal, high fat diet with control, high fat diet with BMB-1 extract) and fed for 15 weeks. Items of this experimental study are as follows: body weight change, final body weight, the weight change of the adipocytes in body, the level change of LFT, NEFA and creatinine, the expression of ${\beta}3AR$ and leptin gene in primary adipocytes, the production change of leptin in primary adipocytes, the expression of ${\beta}3AR$, leptin and $TNF-{\alpha}$ in adipocytes tissue. Result: 1. All experimental groups showedthat the weight change decreased considerably and the high density group showedthat the final weight decreased considerably. 2. The high density group showed that the amount of the adipocyte in weight decreased considerably. 3. All experimental groups showedthat the amount of ALT decreased considerably, and AST decreased in the high density group. However, the amount of creatinine and glucose did not increase considerably. 4. All experimental groups showed that the amount of total cholesterol, LDL-cholesterol, triglyceride, and NEFA decreased, and HDL-cholesterol increased considerably. 5. The high density groups showedthat the amount of leptin decreased considerably. 6. All experimental groups showed that the revelation of ${\beta}3AR$ in primary adipose cell and 3T3-L1 cell increased considerably, and that the revelation of leptin and $TNF-{\alpha}$ in primary adipose cellsand 3T3-L1 cells decreased considerably. 7. All experimental groups showed that the size of adipocyte in adipocytes tissue decreased. 8. All experimental groups showed that the adipose vacuoles in liver tissue decreased considerably. Conclusion: The findings suggest that Bimanbang-1 causes weight loss and histological change, thus it may be effective to treat obesity.

• Journal of Korean Medicine Rehabilitation
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• v.18 no.3
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• pp.51-65
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• 2008

Objectives : In order to investigate the effects of Soongijeseub-bang(Shunqichushi-fang)(here in after referred to SJB) on the obese gene and obese inhibitory, on C57BL/6 mice were induced by high fat diet. Methods : C57BL/6 mice were divided into three groups(normal, high fat diet with control, high fat diet with SJB extract) and fed for 15 weeks. And observed that, body weight change, final increase of body weight, the weight change of the adipocytes, the level change of ALT, AST, creatinine, total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, NEFA, glucose, the expression of ${\beta}3AR$, leptin, and $TNF-{\alpha}$ gene in primary adipocytes and adipocytes tissue. Results : The following results were obtained in this study. 1. SJB 500 mg/kg extract group showed considerable decrease in weight, the final increase of weight and the amount of adipocyte in weight. 2. All experimental group showed that the amount of ALT, AST, total cholesterol, LDL-cholesterol, triglyceride and NEFA were decreased considerably. SJB 500 mg/kg extract group showed the amount of HDL-cholesterol and leptin were increased considerably. 3. All experimental group showed that the revelation of ${\beta}3AR$ in primary adipose cell and 3T3-L1 cell were increased considerably, and that the revelation of leptin in primary adipose cell and 3T3-L1 cell were decreased considerably. SJB $100{\mu}{\ell}/m{\ell}$ extract group showed that the revelation of $TNF-{\alpha}$ were decreased considerably. 4. SJB 500 mg/kg extract group showed that the size of adipocyte in adipocytes tissue were decreased. 5. All experimental group showed that the adipose vacuoles in liver tissue were decreased considerably. Conclusions : Comparison of the results for this study showed that SJB is effective on obesity care and has obese-inhibitory effects in obese mouse induced by high fat diet. So it is respected that the clinical application of SJB can help the treatment of obesity.

• Korean Journal of Oriental Medicine
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• v.14 no.1
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• pp.161-171
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• 2008

Objective : In order to investigate the effects of Soongijeseub-bang(here in after referred to SJB) on the obese gene and obese inhibitory, C57BL/6 mice were induced by high fat diet Methods : C57BL/6 mice were divided into three groups(normal, high fat diet with control, high fat diet with SJB extract) and fed for 15 weeks. And measured body weight change, the weight change of the adipocytes in body, the levels of ALT, AST, creatinine, total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, NEFA(non-esterified fat acid), glucose in serum and the expression of ${\beta}3AR$, leptin, and TNF- ${\alpha}$ gene in primary adipocytes and adipocytes tissue. Results : The following results were obtained in this study. 1. SJB 500 mg/kg extract group showed significant decrease in final weight. 2. SJB 500 mg/kg extract group showed significant decrease in the amount of adipocyte in weight. 3. All experimental group showed significant decrease in ALT and AST levels. 4. All experimetal group did not show significant decrease in glucose and creatinine levels. 5. All experimental group showed significant decrease in total cholesterol, LDL-cholesterol, triglyceride and NEFA levels. HDL-cholesterol were increase significantly in SJB 500 mg/kg extract group. 6. SJB 500 mg/kg extract group showed significant decrease in leptin levels. 7. All experimental group showed significantly increased expressions of fJ 3AR in primary adipose cell and 3T3-Ll cell, and those of leptin in primary adipose cell and 3T3-Ll cell were decreased significantly. SJB $100{\mu}l/ml$ extract group showed significant decrease in the expression of TNF-${\alpha}$. 8. SJB 500 mg/kg extract group showed decrease in the size of adipocyte in adipocytes tissue. 9. All experimental group showed significant decrease in adipose vacuoles in liver tissue.

• Journal of Korean Medicine Rehabilitation
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• v.19 no.4
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• pp.79-93
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• 2009

Objectives : In order to investigate the effects of Gambibang-3(here in after referred to GBB3) on the obese gene and obese inhibitory, C57BL/6 mice were induced by high fat diet. Methods : C57BL/6 mice were divided into four groups(normal, high fat diet with control, high fat diet with Reductil(here in after referred to RDT), high fat diet with GBB3 extract) and fed for 8 weeks. And observed that, the weight change of the adipocytes in body and liver, total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, glucose, leptin change in the serum, the expression of ${\beta}3AR$ and leptin gene in 3T3 cell and primary adipocyte cell. Results & conclusions : The following results were obtained in this study. 1. GBB3 and RDT group showed that the revelation of ${\beta}3AR$ in primary adipose cell and 3T3 cell were increased considerably, and that the revelation of leptin in primary adipose cell and 3T3 cell were decreased considerably. 2. GBB3 and RDT group showed that the weight of adipocyte and liver were not different with control group significantly. 3. GBB3 and RDT group showed that the amount of HDL-Cholesterol were increased considerably, the amount of glucose, LDL-Cholesterol and Triglyceride were decreased considerably. 4. GBB3 group showed that the amount of leptin in the serum were decreased considerably.

• Journal of Korean Medicine Rehabilitation
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• v.20 no.2
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• pp.35-50
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• 2010

Objectives : In order to investigate the effects of So-yangin Biman-bang(here in after referred to BM) on the obese gene and obese inhibitory, C57BL/6 mice were distinctively induced by high fat diet. Methods : C57BL/6 mice were divided into four groups(normal, high fat diet with control, high fat diet with Reductil(here in after referred to RDT), high fat diet with BM extract) and have been fed for 8 weeks. Through observing the process, the weight change of the adipocytes in body and liver, total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, glucose, leptin change in the serum, the expression of ${\beta}3AR$ and leptin gene in 3T3-L1 cell and primary adipocyte cell. Results : The following results have been found in this study. 1. Losing weight have significantly been shown. 2. The weight change of the body adipocyte and liver have been decreased but no significance has been found. 3. With close observation on the change of blood, HDL-cholesterol has positively been increased in numbers but the level of glucose, LDL-cholesterol, triglyceride have distinctively been decreased. 4. The manifestation of ${\beta}3AR$ in 3T3-L1 cell and primary adipocyte cell have shown a definite increase in numbers but the manifestation of leptin has shown certain decrease in its amount. Conclusions : As results of this, So-yangin Biman-bang is thought to be effective in metabolic syndrom and lipid metabolism disorder that can possibly be occurred by obesity control and treatment. The clinical research will be needed in order to apply to So-yangin constitution in the future.

• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.4
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• pp.837-847
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• 2009

In order to investigate the anti-obesity effects of Gambibang-2(here in after referred to GBB2) on the obese gene and obese inhibitory, C57BL/6 mice were induced by high fat diet. C57BL/6 mice were divided into four groups(normal, high fat diet with control, high fat diet with Reductil(here in after referred to RDT), high fat diet with GBB2 extract) and fed for 8 weeks. And observed that, body weight change, the weight change of the adipocytes in body and liver, total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, glucose, leptin change in the serum, the expression of ${\beta}3AR$ and leptin gene in 3T3 cell and primary adipocyte cell, histological analysis of adipose tissue and liver tissue. GBB2 and RDT group showed that the revelation of ${\beta}3AR$ in primary adipose cell and 3T3 cell were increased considerably, and that the revelation of leptin in primary adipose cell and 3T3-L1 cell were decreased considerably. GBB2 and RDT group showed that the body weight were decreased considerably after 7-8 weeks. GBB2 and RDT group showed that the weight of adipocyte and liver were decreased. GBB2 and RDT group showed that the amount of HDL-Cholesterol were increased, the amount of glucose, LDL-Cholesterol and Triglyceride were decreased considerably. GBB2 group showed that the amount of leptin in the serum were decreased. GBB2 and RDT group showed that the size of adipocyte in adipocytes tissue and the adipose vacuoles in liver tissue were decreased. Taking all these observations into account, GBB2 considered to be effective in treating on the obese gene and obese inhibitory. Therefore we have to survey continuously in looking for the effective substance and mechanism in the future.

• Journal of Korean Medicine Rehabilitation
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• v.18 no.2
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• pp.81-96
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• 2008

Objectives : This study was performed to investigate inhibitory effects of Gamipalmul-tang($jiaweibawu-t{\bar{a}}ng$) on the hematological and histological changes of obese mice. Methods : C57BL/6 mice were divided into four groups (normal group, high fat diet with normal saline, high fat diet with reductil, high fat diet with Gamipalmul-tang($jiaweibawu-t{\bar{a}}ng$) and fed for 8 weeks. Body weight change, final increase of body weight, ALT, AST, creatinine, total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, glucose, cell viability by cytotoxicity, the expression of ${\beta}3AR$ in 3T3-L1 cell, the expression of leptin, ${\beta}3AR$ and serotonin in adipocyte tissue and size of adipocyte were observed in 8 weeks. Results : 1. In 3T3-L1 cell. the expression of ${\beta}3AR$ was increased significantly. 2. The final increase of body weight in obese-mouse were decreased significantly. 3. The level of AST, ALT were decreased significantly. 4. The level of LDL-cholesterol was decreased significantly and HDL-cholesterol was increased significantly. 5. The levels of triglyceride was decreased and leptin and glucose were decreased significantly. 6. In adipocyte tissue, the expression of ${\beta}3AR$ were increased significantly. 7. In adipocyte tissue, the expression of leptin and serotonin were decreased significantly. 8. The size of Adipocyte was decreased. Conclusions : On the basis of these results, we concluded that Gamipalmul-tang($jiaweibawu-t{\bar{a}}ng$) has inhibitory effects in rat.

• Korean Journal of Oriental Medicine
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• v.12 no.1
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• pp.77-89
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• 2006

Objective : This experimental study was designed to investigate the effects of Mahwangbokhapbang2 (every abbreviation from now on MHBHB2) on obesity care where it is prescribed for high fat diet-induced mice. Also designed to find out effects of MHBHB2 on controlling th obesity clinically) Method : In order to investigate th obese inhibitory effects of MHBHB2, C57BL/6 mice were induced by high fat diet C57BL/6 mice were divided into four group(normal, high fat diet, high fat diet with reductil, high fat diet with MHBHB2 extract) and fed for 15weeks. Result : 1. Mahwangbokhapbang2(MHBHB2) decreased significantly the body weig-ht. 2. MHBHB2 decreased significantly the weight of adipocyte. 3. MHBHB2 decreased significantly the blood level of serum ALT, AST. 4. MHBHB2 decreased significantly the blood level of serum total cholester-l, LDL- cholesterol, friglyceride and NEFA(free fatty acid). 5. MHBHB2 decreased significantly the blood level of HDL-cholesterol. 6. MHBHB2 500mg/kg decreased significantly the blood level of Leptin. Conclusion : Based on these results, it is prove that MHBHB2 is effective on obesity care and has obese-inhibitory effects in obese-mouse induced by high fat diet. So it is espected that the clinical application of MHBHB2 can help the treatment of obesity.

• Journal of Physiology & Pathology in Korean Medicine
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• v.21 no.3
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• pp.634-641
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• 2007

In order to investigate the effects of Bogigambi-tang(here in after referred to BGGBT) on the obese gene and obese inhibitory, C57BL/6 mice were induced by high fat diet. C57BL/6 mice were divided into three groups(normal, high fat diet with control, high fat diet with BGGBT extract) and fed for 15 weeks. Items of this experimental study are as follows. Body weight change, final inclose of body weight, the weight change of the adipocytes in body, the level change of ALT, AST, total cholesterol, LDL-Cholesterol, triglyceride, glucose, free fatty acid and creatinine, the expression of ${\beta}$3AR and leptin gene in primary adipocytes, the production change of leptin in primary adipocytes, the expression of ${\beta}$3AR and leptin in adipocytes tissue. The following results have been obtained All experimental group have shown that the weight and the final increase of weight have decreased considerably. All experimental group have shown that the amount of the adipocyte in weight has decreased considerably. All experimental group have shown that the amount of leptin has decreased considerably. All experimental group have shown that the revelation of ${\beta}$3AR in primary adipose cell and 3T3-L1 cell has increased considerably, and that the revelation of leptin in primary adipose cell and 3T3-L1 cell has decreased considerably, All experimental group have shown that the size of adipocyte in adipocytes tissue has decreased. The high density group have shown that the adipose vacuoles in liver tissue has decreased considerably, and that the cell nucleuses has similar with normal group.