• 한국식품저장유통학회지
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• 제30권3호
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• pp.383-394
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• 2023

The ultra-fast quantitative real-time polymerase chain reaction (qPCR) assay was developed and validated to differentiate the morphologically similar ones, Oncorhynchus keta and Oncorhynchus mykiss. Species-specific primers were designed for the COI genes of mtDNA. The species-specific primers designed for O. keta and O. mykiss were selectively amplified by O. keta and O. mykiss DNA, respectively. The sensitivity of O. keta and O. mykiss primers was 1 ng/μL. Quantitative testing showed that the results met the 'Guidelines on Standard Procedures for Preparing Analysis Method such as Food' proposed by the Ministry of Food and Drug Safety. The qPCR method developed and validated in this study for identifying O. keta and O. mykiss has advantages such as speed and field applicability. Therefore, this method is expected to help control forgery and alteration of raw materials in the seafood industry.

• 대한두개안면성형외과학회지
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• 제19권1호
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• pp.20-34
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• 2018

Background: Polydeoxyribonucleotide (PDRN) influencing cellular growth and differentiation is recognized to promote wound healing by stimulating tissue repair. Although PDRN can be extracted from human placentas, PDRN medications have recently been extracted from the semen of trout (Oncorhynchus mykiss) and salmon (Oncorhynchus keta). The present study was designed to evaluate the wound healing effects of O. keta-derived PDRN for injection (Rejuvenex) and PDRN cream (Rejuvenex Cream) in comparison with those of O. mykiss-derived PDRN injection (Placentex). Methods: Full-thickness skin defects were made on the back of mice (n=60). The mice were divided into the following four groups according to the dressing used for the wounds: O. mykiss-derived PDRN injection group, O. keta-derived PDRN injection group, O. ketaderived PDRN cream group, and normal saline soaked dressing group (control group). We analyzed the gross findings, wound sizes, histological findings, immunohistochemistry and enzyme-linked immunosorbent assays for the groups immediately after the treatment, and again after 4, 7, and 10 days of treatment. Results: The wound healing effects were the greatest in the O. keta-derived PDRN injection and O. mykiss-derived PDRN injection groups, which showed similar scores, followed by the O. keta-derived cream and normal saline soaked dressing groups. Conclusion: The injection of PDRN extracted from O. keta was found to be as effective at healing full-thickness skin defects as the O. mykiss-derived PDRN injection, which is currently used in the clinic. Moreover, the O. keta-derived PDRN injection was also found to reduce the time required for wound healing.

• 한국어류학회지
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• 제5권1호
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• pp.96-112
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• 1993

한국산 연어속에 속하는 연어, 산천어 및 무지개송어 3종의 형태학적 비교 연구를 하였다. 연어속 어류인 연어, 산천어, 무지개송어의 계측, 계수형질을 비교한 결과 체형에선 암컷이 두장을 비롯한 13개, 수컷이 11개 형질에서 차이가 있었고, 계수 형질에선 유문수와 새파수에서 가장 뚜렷한 차이를 나타내었다. 이들 세 종은 암, 수컷 모두에서 표준체장에 대한 체고, 미병고, 등지느러미 길이, 뒷지느러미의 길이와 두장에 대한 주둥이 길이, 안경, 위턱, 뺨의 비에서 차이를 보였다. 이중 두장에 대한 안경은 암수 모두에서 산천어 무지개송어, 연어 순으로 크게 나타나 3종을 분류할 수 있는 새로운 형질로 나타났다. 유문수 수는 연어가 103~236개(평균 151개)로 무지개송어 37~84개(평균 56개), 산천어 23~63개(평균 41개)로 종간에 좋은 분류 형질로 나타났다. 본 연구에 나타난 체형 및 계수 형질 분석 결과 산천어는 연어와 무지개송어의 중간 형질을 가지고 있는 종으로 나타나, Smith and Stealy(1989)의 계통수와 잘 일치하였다.

• 미생물학회지
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• 제22권2호
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• pp.85-90
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• 1984

Two viruses were isolated from kidney and spleen tissues of goldfish(Carasius auratus) and the ovarian fluid of chum salmon(Oncorhynchus keta). Both viruses replicated and produced cytopathic effect in EPC, CHSE-214, and CHH-1 cell lines at $15^{\circ}C$. The isolates were resistant to pH 3 and choloroform. Antiserum to infections pancreatic necrosis virus(IPNV) serotype VR 299 neutralized the infectivity of both of the isolates. Electron microscopy showed that the particles had typical IPNV particle morphology with average diameters of 55nm, This paper describes the first isolation of viruses infecting cultured fish in Korea.

• 환경생물
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• 제40권4호
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• pp.651-659
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• 2022

본 연구는 연어(Oncorhynchus keta) 치어의 성장, 생존 및 혈액 성상에 미치는 사육수온(8, 11, 14 및 17℃)의 영향을 연구하기 위해 수행되었다. 실험 종료 시 연어의 체중(body weight, BW)은 14℃ 실험구에서 가장 높았을 뿐 아니라, 특이성장률(specific growth rate, SGR), 사료전환 효율(feed conversion ratio, FCR), 사료효율(feed efficiency, FE), 증중률(weight gain, WG) 및 비만도(condition factor, CF)와 같은 성장지표에서도 14℃ 실험구가 가장 높은 경향을 나타내었다. 생존율(survival rate)은 8℃와 11℃ 실험구에서 100%를 보였고, 14℃ 실험구에서는 96% 및 17℃ 실험구에서는 98%를 각각 나타내었다. 혈장 성분 중 알라닌 아미노전이효소(alanine aminotransferase, ALT)는 17℃ 실험구에서 유의한 감소를 보였으나, 알부민(albumin), 총단백질(total protein, TP), 나트륨(sodium, Na⁺), 칼륨(potassium, K⁺) 및 염소(chloride, Cl^-)는 유의한 변화를 보이지 않았다. 실험 종료 시 연어의 체성분 조사를 실시한 결과, 수분(moisture), 단백질(crude protein) 및 회분량(crude ash)은 수온에 따른 유의한 변화는 보이지 않았으나, 지질(crude lipid)은 다른 실험구에 비해 8℃ 실험구에서 유의하게 높은 경향을 보였다. 결론적으로 연어 치어가 성장하는데 필요한 최적의 사육 수온은 14℃로 보이나, 혈액 성상 등 생리적인 영향은 향후 추가적인 연구가 필요한 것으로 판단된다.

• Fisheries and Aquatic Sciences
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• 제18권1호
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• pp.13-20
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• 2015

Calculated chemical scores (computed in relation to the FAO/WHO reference protein) for salmon liver protein hydrolysates indicated that all amino acids (other than methionine and threonine) were present in adequate or excess quantities; thus, the raw liver material is a good source of essential amino acids. The hydrophobic amino acids contents in hydrolysates prepared from Oncorhynchus keta and O. gorbuscha were 38.4 and 39.1%, respectively. The proportion of released peptides exceeding 500 kDa was reduced when hydrolysates were treated with the commercial enzyme Alcalase, although proportions in the following MW ranges were elevated: 100-500 kDa and <50 kDa. The optimal conditions for enzymatic hydrolysis were as follows: pH 7.0, $50^{\circ}C$, and a reaction time of 1 h. Of the different proteases tested, Alcalase was the most efficient for production of salmon liver hydrolysate with the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity. The hydrolysates prepared from salmon liver had a balanced amino acid composition. The liver protein hydrolysates contained low molecular weight peptides, some of which may be bio-active; this bio-active potential should be investigated. Inhibition of the DPPH radical increased with increased degree of hydrolysis (DH), regardless of protease type. DPPH radical scavenging abilities, antithrombotic effects and ${\alpha}$-glucosidase enzyme inhibition effects of O. keta liver hydrolysate increased in a dose-dependent manner. Thus, salmon liver hydrolysate may be useful in functional food applications and as a source of novel products.

• 수산해양교육연구
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• 제26권2호
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• pp.421-429
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• 2014

We investigated the feeding ecology of juvenile chum salmon (Oncorhynchus keta) during the critical early life stage prey selectivity of juvenile chum salmon during early marine migration in Korean waters at spring 2011. Salmon juveniles and zooplanktons were collected to draw with $20m{\times}5m$ gill net and $300{\mu}m$ mesh zooplankton net at each station on 11th-13th April n 2011. Collected zooplanktons were classified to 5 Phylum, 6 Class, 9 Order 17 Species in this study. Almost 76.4-100% species were classified to Phylum Arthropoda, dominant species was a species out of Hyperia galba of Order Amphipoda, Acartia spp and Paracalanus parvus of Order Calanoida. Collected salmon juveniles were grew up to average 4.7-5.4 cm fork length and average 1.0-1.5 g wet weight in whole station. Fish stomach content (mg/salmon) was heaver to 97.4, 82.4 and 63.2 mg wet weight/salmon in ST 2, 3, 4 than 20.4, 18.9 mg/salmon of ST 1, 5, because there are fish (sand eel, Hypoptychus dybowskii) and Krill (Euphausia) as prey in salmon stomach in ST 2, 3, 4. And ST 2, 3, 4 and 5 were dominated by Amphipoda as Hyperia galba, Themisto japonica and Gammarus sp., but ST 1 was dominated by copepod, because of absence of Amphipoda in the station. Therefore small Amphipoda as Hyperia galba was good prey for just released salmon juvenile in nature.

• 한국양식학회지
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• 제11권3호
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• pp.363-369
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• 1998

본 연구에서는 북해도의 연어(Oncorhynchus keta) 치어에 있어서 1일 4회, 1일 2회, 1일 1회, 하루건너 4회 및 하루건너 2회의 각기 다른 사료 공급 조건에 의한 성장을 비교 분석하였다. 또한, 주 1회 및 2회의 사료공급 중지에 따른 영향을 조사하였다. 치어의 성장은 배일 2회 및 4회의 사료공급군이 다른 실험군에 비해 성장률, 비만도, 사료전환효율을 및 생존율에서 좋은 성장 결과를 나타내었다. 그리고 주 5회 사료공급한 실험군보다 매일 공급 및 주 6회 사료공급한 실험군이 좋은 성장을 나타내었다. 어류의 간세포 핵의 크기는 매일 공급한 실험군이 큰 수치를 나타내어 체내물질대사가 좋은 것으로 나타났다. 이러한 결과로 보아, 연어 치어의 사육에 있어서는 주 6일이상, 1일 2회이상의 사료공급이 적합한 것으로 판단된다.

• 한국수산과학회지
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• 제36권6호
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• pp.578-585
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• 2003

The chum salmon (Oncorhynchus keta) is an anadromous fish distributed all around the North Pacific. Artificial production and release of the juveniles are being made by Korea, Japan, Russia, Canada and the United States. It is important to set up some criteria identifying each stock in order to clarify each nation's right of harvest for the chum salmon resource. As an attempt to build such criteria, we analyzed sequences of a microsatellite DNA Ogo5 and the COIII-ND3-ND4L region of the mitochondrial DNA from chum salmons of Korea, Japan, and the United States. Ogo5 has 4 different alleles: allele A, B-1, B-2, and B-3. Allele B-3 is found only in 3 individuals out of 12 Korea salmons. The Japan salmons have the other 3 alleles and the America salmons have only 2 allots, A and B-1. Heterozygosity index (Ho/He) distinguishes the Korea (1.61) and Japan salmons (1.63) from the America ones (1.09). Seventeen different haplotypes are found in the COIII-ND3-ND4L region from 60 individuals,20 from each stock. The gene genealogy of the haplotypes revealed by TCS program shows that the Korea and Japan salmons are genetically closely linked, but that they are clearly distinguished from the America ones. Ten and eleven individuals of the Korea and Japan salmons have an identical haplotype. Nine individuals of the Korea salmons $(45\%),$ however, are separable from the Japan salmons by their own specific nucleotides. This result presents usefulness of the COIII-ND3-ND4L region as a genetic marker for identification of the chum salmon stocks.

• 한국수산과학회지
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• 제56권5호
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• pp.734-740
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• 2023

The effect of feeding frequency on growth performance, body nutrient composition, and hematological parameters of juvenile chum salmon Oncorhynchus keta was investigated. The juveniles (10.9±0.3 g) were fed commercial feed for 30 days with feeding frequencies of 1, 2, 3, 4, or 5 meal (s)/day (n=2 tanks per treatment) in a recirculating aquaculture system (RAS). Fish were fed to satiation at each of the feeding frequencies. At the end of the feeding trial, final body weight, weight gain, and specific growth rate of fish fed 2 meals/day were significantly higher than those of fish fed 1 meal/day(P<0.05); however, no difference was detected among the other feeding frequencies. Daily feed intake significantly increased with increasing feeding frequency, whereas feed and protein utilization efficiencies continuously decreased with increase in the feeding frequency. Among the treatments, fish fed 1 meal/day showed the lowest value in whole-body lipid content and total protein concentration in plasma. Taken together, the optimal feeding frequency for growth performance and feed utilization efficiency in chum salmon juveniles reared in the RAS was determined to be 2 meals/day.