• Natural Product Sciences
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• v.23 no.3
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• pp.217-221
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• 2017

Eleven compounds (1-11) were isolated from the rhizomes of Osmunda japonica, and their structures were elucidated based on $^1H$, $^{13}C-NMR$ and LC-IT-TOF MS data. Of these compounds, all compounds (1 - 11) have been previously reported, although five (6 - 9, 11) have not previously been isolated from this plant. The antioxidant activities of isolated compounds (1 - 11) were measured by DPPH and ABTS assays, and compound 10 showed the high antioxidant activity.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2007.11a
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• pp.343.2-343.2
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• 2007

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• Korean Journal of Pharmacognosy
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• v.47 no.3
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• pp.232-236
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• 2016

In traditional Korean medicine, Osmunda japonica Thunb has been used as hemostasis and antipyretic treatment. The main compound "cinnamtannin B-1" was obtained by column chromatographic separation, and its structure was determined by spectroscopic methods, including $^1H$, $^{13}C$ NMR, and IT-TOF-ESI MS. Ash, moisture and extract content and acidinsoluble ash were monitored as identification test to establish the analytical methods. The optimum reflux extraction condition was 100% methanol extracted 30 min for 2 times. A quantitative analysis using HPLC method exhibited that the main compound at 24.7 min and its content was 0.96% in methanol extraction.

• Korean Journal of Plant Resources
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• v.24 no.2
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• pp.174-180
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• 2011

This study was conducted to investigate the optimum condition of extraction from fronds of Osmunda japonica to increase antioxidant compounds and antioxidant activity. Powder (1 g) of lyophilized fronds were mixed with three different solvents (MeOH, 80% EtOH and water). Extraction was carried out using not only by immersion (room temp.), heating ($60^{\circ}C$) and stirring (200 rpm) for 6 h, but also by sonication in 42 kHz ultrasonic bath for 15, 30 and 45 min. Extracts were filtered, and adjusted up to 50 mL to determine contents of soluble solids, total polyphenols and total flavonoids. Antioxidant capacity was measured by radical scavenging activity of 0.15 mM DPPH (2,2-diphenyl-1-picrylhydrazyl) and 7.4 mM ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] radical. Among the solvents, MeOH and 80% EtOH appeared to be effective for extraction. Extract obtained from sonication in MeOH for 15 min resulted high polyphenol contents (45.15 $mg{\cdot}g^{-1}$ db) and DPPH radical scavenging activity ($RC_{50}$= 0.35 $mg{\cdot}mL^{-1}$). The highest flavonoid contents was obtained from immersion or heating extraction with MeOH (38.10~38.10 $mg{\cdot}g^{-1}$ db). ABTS radical scavenging was high in same extraction with 80% EtOH ($RC_{50}$= 0.21~0.22 $mg{\cdot}mL^{-1}$). Altogether, our results indicate that the extraction using ultrasonic bath with MeOH as a solvent (for 15~30 minutes) was the most effective way not only for increasing various antioxidant activities but also for saving labor and time in case of fronds of Osmunda japonica.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2007.11a
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• pp.349.1-349.1
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• 2007

• Journal of Environmental Science International
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• v.20 no.4
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• pp.443-455
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• 2011

This study was conducted to investigate the flora of Mt. Jang located at Haeundae-Gu in Busan. The survey for the flora was carried out from Oct. 2008 to Sep. 2009. The results were obtained as follows. The flora consists of 5 forms, 22 varieties, 238 species, 198 genera, 91 families, 47 orders, 7 classes, and 3 divisions as total 265 taxa. The typical communities were Osmunda japonica com., Stephanandra incisa com., Gleichenia japonica com., Eurya japonica-Pinus thunbergii com., Styrax japonica-Pinus thunbergii com., and Smilax china com. which were distributed along Forest Bathing Area and Yangun Falls, Miscanthus sinensis for. purpurascens com., Carpinus turczaibovii var. coreana com and Pseudosasa japonica were distributed around the top of mountain. According to the ecological characteristic species, the evergreen plants were observed 15 taxa 14 genera 13 families, the naturalized plants 18 taxa 17 genera 10 families, the specific plants categorized by Ministry of Environment 20 taxa 18 genera 18 families, the endangered plant 1 specie Utricularia yakusimensis, and the cultivated plants were observed 21 taxa 18 genera 14 families.

• Biomolecules & Therapeutics
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• v.11 no.4
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• pp.245-248
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• 2003

The purpose of this study was to evaluate tyrosinase inhibitory activity of plant extracts, especially Korean indigenous plants, for the cosmetic use of skin whitening. When 50 plant extracts were tested, the methanol extracts of Agrimonia pilosa, Aster scaber; Dianthus sinensis, Fatsia japonica, Hemistepta lyrata, Lespedeza cuneata, Osmunda japonicum, Pyrvla japvnica, Rodgersia phodophylla and Veratrum grandiforum possessed the considerable tyrosinase inhibitory activity at 3-300 $\mu\textrm{g}$/mL. Especially, L. cuneata, aerial part of O. japonicum and V. gandiforum exhibited the strong inhibition (>50％ inhibition at 300 $\mu\textrm{g}$/mL). In particular, the methanol extract of V. grandiforum and its ethylacetate fraction showed potent inhibition ($IC_{50}$/=30 and 13$\mu\textrm{g}$/mL, respectively), while the reference compound, kojic acid, showed $IC_{50}$/ value of 26$\mu\textrm{g}$/mL. These plant extracts may be used as tyrosinase inhibitors in cosmetics.

• Korean Journal of Plant Resources
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• v.24 no.4
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• pp.351-357
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• 2011

This study was carried out to investigate the effective extraction condition for increase of phytoecdysteroids from fronds of Matteuccia struthiopteris (FMS) and Osmunda japonica (FOJ). Lyophilizated fronds were mixed with three different solvents (MeOH, 80% EtOH or water) and then extraction was carried out by using six different methods, such as, immersion (room temp.), heating (60$^{\circ}C$), stirring (200 rpm) for 6 h, or sonication in 42 kHz ultrasonic bath for 15, 30 and 45 minutes. Contents of 20-hydroxyecdysone (20E) and ponasterone A (PonA) were measured by using HPLC after purification of the extracts by $C_{18}$ cartridge. Altogether, our results indicate that the extraction using sonication with MeOH as a solvent (for 30 minutes) was the most effective condition for 20E and PonA from both MFS and FOJ. Resulting contents of 20E from FMS and FOJ were 66.76 and 104.48 ${\mu}g{\cdot}g^{-1}$ and PonA were 53.43 and 43.82 ${\mu}g{\cdot}g^{-1}$, respectively.

• Biomolecules & Therapeutics
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• v.11 no.3
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• pp.196-199
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• 2003

The methanol extracts from 60 plant extracts were prepared and evaluated for their antioxidative activity. Arctium lappa, Diospyros kaki, Eugenia caryophyllata, Melia azedarach and Forsythia suspensa showed the inhibitory activity against lipid peroxidation. Caesalpinia sappan, Crataegus pinnatifida, Eugenia caryophyllata, Gleditsia japonica, Osmunda japonica, Rhus javanica and Sanguisorba officinalis showed the highest inhibitory activity against DPPH radical formation. In particular, Eugenia caryophyllata demonstrated strong inhibitory activity on the lipid peroxidation and free radicals. The results suggested that selected plant extracts have a potential as natural antioxidant.

• The Korean Journal of Pesticide Science
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• v.8 no.3
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• pp.220-230
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• 2004

This study was conducted of Korean native plants to screen herbicidal activity which could be used for the development of new natural herbicides. Ninety-eight plants were collected from Wan Island, Chollanamdo in Korea and their methanol extracts were obtained. Herbicidal activities of the methanol extracts were determined by seed bioassay using canola (Brassica napus L.) seedlings. Among ninety-eight species, twenty plants were highly herbicidal ($GR_{50}<1,000\;{\mu}g\;g^{-1}$): Abies holophylla MAXIM., Ailanthus altissima (MILL.) SWINGLE, Anthemis nobilis L., Aralia elata SEEM., Artemisia iwayomogi KITAMURA, Asarum sieboldii MIQ., Brassica campestris subsp. napus var. nippo-oleifera MAKINO, Clematis terniflora DC., Crataegus scabrida SARG., Gnaphalium affine D. DON, Jasminum nudiflorum LINDL., Kalopanax pictus (THUNE.) NAKAI, Machilus japonica S. et Z., Myrica rubra S. et Z., Osmunda japonica THUNB., Phytolacca esculenta V. Houtte, Platanus occidentalis L., Quisqualis indica L., Rubus hirsutus THUNB., Yucca smalliana FERN. Fifty plants were shown moderate herbicidal activity $(1,000\;{\mu}g\;g^{-1}, however, twenty-eight plants were not shown any herbicidal activity.