• Development and Reproduction
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• v.20 no.2
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• pp.65-79
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• 2016

We observed the osteological development of larval and juvenile red spotted grouper (Epinephelus akaara) in order to generate data for the assessment of skeletal deformities and to inform phylogenetic systematics research. Larvae and juveniles were obtained from a aquafarm in Muan-gun, Jeolla-namdo Province, Korea. The average water temperature at the time of breeding was $23.0^{\circ}C$ and average water salinity was 33.0 psu. Freshly hatched fish larvae had not undergone any ossification, but ossification of the parasphenoid bone, which forms the base of the cranium, occurred as the juveniles reached an average body length (BL) of 2.49 mm. At the same time, ossification of the preopercle and opercle occurred in the operculum, and ossification of the maxilla, which forms the upper jaw, and the dentary bones, which form the lower jaw, began. In addition, ossification of the vertebra occurred by formation of 7 vertebral centra and the neural spine in the abdominal vertebra. When the juveniles reached an average (BL) of 5.22 mm, ossification of the nasal, lateral ethmoid, and alisphenoid bones occurred in the cranium; ossification of the endopterygoid and metapterygoid bones began in the palatine region; and ossification of the hypohyal and interhyal bones occurred in the hyoid arch. At an average (BL) of 20.9 mm, ossification of the basisphenoid bone in the cranium and the suborbital bone in the orbital region occurred. Ossification of the vertebra then occurred by the formation of long pairs of ribs from the third to the ninth abdominal vertebrae, completing osteological development.

• Journal of Korean Foot and Ankle Society
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• v.13 no.1
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• pp.99-102
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• 2009

Heterotopic ossification of Achilles tendon is known to be related with history of prior Achilles tendon surgery, trauma, Achilles tendon rupture. We report a case of heterotopic ossification of partially ruptured Achilles tendon and treated by surgical removal of ossification and V-Y advancement with tendon repair.

• Imaging Science in Dentistry
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• v.39 no.1
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• pp.7-11
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• 2009

Purpose: The purpose of this study was to assess the prevalence and radiographic characteristics of the nuchal ligament ossification on lateral cephalometric radiographs in Koreans. Subjects and Method: I review and interpreted the lateral cephalometric radiographs from 4,558 patients (1,857 males and 2,701 females, age range from 2 to 79 years) who visited the Kyungpook National University Dental Hospital from January 1, 2008 to February 3, 2009. I grouped the shapes of nuchal ligament ossification as round, rod-like, and segmented shape. And localized the ossification as the involvement of anterior cervical vertebral body. The data were analyzed by using chi-squared test with two-tailed and at a 5% significance level. Results: Among those who showed the nuchal ligament ossification, he mean age of the 143 males was 51.1 and that of the 97 females was 48.0 years. It as not observed completely below teens, and was observed 1% in twenties, 6.1% in thirties, 18.6% in forties, and 26.3% over fifties. It was significantly prevalent in older age group (P<0.01) and in males than females among the same age group (P<0.05). The shapes of nuchal ligament ossification were as follows in order of frequency: rod-like (49.2%), round (30.4%), and segmented (20.4%). The highest involvement of ossification as found at the level of C5 (67.9%), C4 (29.2%), C6 (22.9%), C3 (3.3%), C7 (2.9%), C2 (0.8%), and C1 (0.4%). Conclusion: The nuchal ligament ossifications on lateral cephalometric radiographs were showed as round, rod-like, or segmented shape. The nuchal ligament ossification is often observed after the age of 40 and is observed more frequently in males than females. The highest shape of nuchal ligament ossification was rod-like shape and the highest involvement of cervical spine was C5.

• Journal of Korean Foot and Ankle Society
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• v.6 no.2
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• pp.221-223
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• 2002

Ossification of the Achilles tendon is a very rare condition. We report a case with ossification of Achilles tendon, recently treated surgically. The patient was a 44 year old male whose chief complaint was discomfort around the Achilles tendon. He didn't have a previous history of surgery or trauma. The roentgenography showed that the bony mass was $15\times3cm$ on the right leg. Ossification of Achilles tendon was found in the Achilles tendon and treated by surgical removal of a bony mass and suturing the tendon. Microscopic examination of the extirpated specimen revealed bone formation through enchondral and intramembranous ossification in the Achilles tendon.

• The korean journal of orthodontics
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• v.7 no.1
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• pp.13-22
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• 1977

The author investigated the timing of land and wrist ossification events taking place in the hamate and pisiform by means of roentgenographic examination and its relation to the peak growth velocities in body height. Materials obtained included 493 sheets of X-ray films taken from 257 boys ana 236 girls aged between 7 and 17 years lived in Seoul area. The results indicate that; 1) The average ages at occurrence of initial ossification of the hook of the hamate were 12 years 3 months in boys and 10 years 1 month in girls. 2) The average ages at which advanced ossification of the hook of the hamate occurred were 13 years 7 months in boys and 11 years 11 months in girls. 3) Initial ossification of the pisiform appeared at 13 years 0 month in boys and 10 years 2 months in girls. 4) All three ossification events in hand and wrist, and peak growth velocity in body height occurred earlier in girls than in boys. 5) Initial ossification in the hook of the hamate and pisiform preceded or coincided with peak growth in body height in most boys and girls, on the other hand, advanced ossification in the hook of the hamate occurred around peak growth in body height but in girls did it after peak growth in body height. 6) It would appear that initial ossification in the hook of the hamate or in the pisiform, both for boys and girls, provides a better indication of approaching peak adolescent growth in body height. 7) Advanced ossification in the hook of the tamale would indicate that the period of cercumpuberal peak growth had teen entered or finished.

• Applied Microscopy
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• v.26 no.2
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• pp.177-195
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• 1996

Fine structure of the processes of intramembranous ossification and endochondral ossification at the tip of the distal phalanx of human fetuses was studied by electron microscopy. In 50 mm fetus, intramembranous ossification of the tip of cartilaginous phalanx was first noted. The osteoblasts of the perichondral zone of tip of cartilaginous phalanx started to lay down a thick membranous bony lamella. Most of the hypertrophied chondrocytes in the marginal parts of tip of the distal phalanx remained viable after being embeded in mineralized cartilaginous septa. The tuberosity of the distal phalanx was formed by membranous bony trabeculae on the exterior of the subperiosteal cap at 80 mm fetus. At this stage endochondral ossification was first observed in distal extremity of the distal phalanx. The maority of hypertrophied chondrocytes in the center of distal extremity appeared to be disintegrating. Resorption of calcified matrix was undertaken by perivascular cells and chondroclasts. From the periosteum, zone of calcification, vascular sprouts expanded within a recently opened lacunae, and the invading osteoblasts laid down osteoid and bone. After 120 mm fetus, endochondral and subperiosteal ossification proceeded in only one direction, just proximally. These findings demonstrate that intramembranous ossification, calcification, and endochondral ossification start at tip of the distal phalanx instead of at the center of the shaft, as was the case in other long bones.

• Journal of Korean Academy of Oral and Maxillofacial Radiology
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• v.1 no.1
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• pp.21-27
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• 1971

The author investigated the carpal bone and ephiphyses on the roentgenographic films. Several methods have been used in the study of carpal bones but I have calculated the numbers of ossification center. The subjects consisted of 259 boys and 239 girls from 3 to 13 years of age. The results are summerized as follows; 1. The number of ossification center were increased in aging both boys and girls. 2. Ossification tendency was more faster boys than girls and the difference of left and right hand were non significant. 3. The ossification of ephiphyses was completed in 4 years of age.

• Journal of Environmental Health Sciences
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• v.27 no.2
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• pp.73-81
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• 2001

The purpose of this study was to determine the adverse effects of methylmercuric chloride(MMC) against the fetal growth and the ossification rate of fetal pectoral and pelvic girdle, stermebrae, ribs and tail in pregnant Fischer 344 rats administered orally on day 7 of gestation. The resulted obtained are as follows. The weight and size of fetus were highly reduced by MMC. The reduction of fetal weight and size were 16. 2%~24.5%(p<0.01), and 34.1%~48.8%(p<0.01), and that of the litter’s weight were 67.0%(p<0.01) and 89.2%(p<0.01) by 20 and 30mg/kg MMC, respectively. Ossification centers were never formed in pectoral and pelvic phalanges and sternebrae, and was reduced as much as 70% in tail by 30mg/kg MMC. And also those were 82.4%~ 91.2%(p<0.01) in ischium, and 52.4~66.7%(p<0.01) in the others(ilium, fenur, tibia, fibula, metatarsals)of pelvic girdle by 30 mg/kg MMC. Ossification of sternebrae was terrible. 5th bone of sternebrae was not ossificated by 20 and 30 mg/kg MMC(p<0.01), and 2nd was also not ossificated by 30 mg/kg MMC(p<0.01).And reduction of ossification rate was 84.8~97.8%(p<0.01) in the others of sternebrae by 30 mg/kg MMC. And then, the reduction of ossification rate was 26.65~49.8%(p<0.01) in fetal ribs by 30 mg/kg MMC, and they were trend to increased as following from center to each edge. In conclusion, it was observed that fetal weight, size, and ossification of each bone were highly significantly reduced by the increased dosage of MMC.

• Journal of Korean Foot and Ankle Society
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• v.10 no.1
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• pp.88-91
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• 2006

Purpose: To evaluate the effect on clinical course of heterotopic ossification of distal tibiofibular syndesmosis after ankle fractures. Materials and Methods: From June 2001 to May 2004, we found nine cases of heterotopic ossification of distal tibiofibular syndesmosis after ankle fractures. There were 8 male patients and 1 female patient; their mean age was 42 years old. There were 6 Weber type B and 2 Weber type C fractures, and there is 1 case with posterior malleolus fracture only. Among them, 8 ankle fractures were operated. Follow up period was averaged for 14 months. We were able to review radiographs at initial injury and to review clinical menifestation and radiographs at last follow up. We used an ankle-hindfoot scoring system of AOFAS which combined symptom, function and alignment with maximum score of 100 point. Results: In all cases ankle dorsiflexion and plantarflexion were not significantly different from that of the contralateral side. The patients who had developed heterotopic ossification in distal tibiofibular syndesmosis had a similar functional score. The mean ankle-hind foot score was 94 points. Conclusion: We concluded that the heterotopic ossification of distal tibiafibular syndesmosis after ankle fractures had little effect on clinical course and range of motion of ankle joint.

• Applied Microscopy
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• v.24 no.1
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• pp.59-76
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• 1994

Fine structure of the distal femoral epiphysis of growing mouse was studied by electron microscopy. The first morphological evidence of developing secondary center of ossification in the distal femoral epiphysis was found at newborn mouse. Ossification center was in the form of multiple foci of calcification and its cells were represented by remnant of degenerated cells within large lacunae that were separated by mineralized cartilaginous septa. Endochondral ossification beneath the articular cartilage proceeded in a less orderly manner than metaphyseal endochondral ossification. Columns of hypertrophied chondrocytes were not distinctly parallel to intercellular mineralized septa in all direction. Hypertrophied chondrocytes in the inner zone of the epiphseal center of ossification showed disintegrated. Resorption of mineralized cartilaginous septa was undertaken by perivascular cells and multinucleated chondroclasts. Resorption of the calcified cartilage was restricted to the region of ruffled border of the chondroclast. Growth along the metaphyseal side of the epiphyseal center of ossification was different from that along the articular surface. As the secondary center expanded toward the metaphyseal side, many vascular buds penetrated unmineralized cartilaginous septa and invaded viable chondrocytes. Many hypertrophied chondrocytes bodering the metaphyseal side of bone center remained viable after they became embedded in mineralized cartilaginous septa. This result suggested that the hypertrophied.