• Journal of Korean Society of Steel Construction
• /
• v.17 no.3 s.76
• /
• pp.347-356
• /
• 2005

Recently, pre-engineering building (P.E.B.) systematic frames are increasingly being used in steel factory buildings, but almost of the related techniques are dependent on the engineering program (e.g, MBS, LTI), which is usually imported from other countries. These are designed under the AISC-ASD because at present there is no Korean design code for P.E.B. frames. Also, there are few studies onbehaviour and we need to develop the element techniques by using H-shaped components.In particular, there is a tendency towards overestimated design because column-rafter connections have been designed with extended end plate type joint, which is treated asrigid joint,so structural examinations are needed. Therefore, this study represents a basic step in ascertaining the application of P.E.B. systematic frames by using H-shaped column-rafter connectionwith flush type end plate. Its structural performance is compared with that of existing extended type joint using a structural performance test. The structural behaviour of specimen was understood qualitatively and the possibility of application (e.g, design aid charts) of semi-connection (flush type) with H-shaped column-rafter was determined.

• Journal of Korean Society of Steel Construction
• /
• v.19 no.5
• /
• pp.549-558
• /
• 2007

Hook bolt brace is being used to construct pre-engineered building's (PEB) systematic frames in steel factory building, but they pose difficulties in terms of their structural performance. There are also few studies on their behavior and there is a need to develop elementary techniques by conducting loading tests on other types of braces (e.g., rod bar, rod shoe, angle), which is used in Japan and the USA. Its structural performance is compared with that of current hook bolt-type brace. Therefore, in this study, we present a basic step in the structural performance of these specimens to examine its performance qualitatively and theof application to PEB frames.

• Journal of Information Processing Systems
• /
• v.12 no.1
• /
• pp.46-56
• /
• 2016

Dynamic textures are videos that exhibit a stationary property with respect to time (i.e., they have patterns that repeat themselves over a large number of frames). These patterns can easily be tracked by a linear dynamic system. In this paper, a model that identifies the underlying linear dynamic system using wavelet coefficients, rather than a raw sequence, is proposed. Content based threshold filtering based on Set Partitioning in a Hierarchical Tree (SPIHT) helps to get another representation of the same frames that only have low frequency components. The main idea of this paper is to apply SPIHT based threshold filtering on different bands of wavelet transform so as to have more significant information in fewer parameters for singular value decomposition (SVD). In this case, more flexibility is given for the component selection, as SVD is independently applied to the different bands of frames of a dynamic texture. To minimize the time complexity, the proposed model is implemented on a graphics processing unit (GPU). Test results show that the proposed dynamic system, along with a discrete wavelet and SPIHT, achieve a highly compact model with better visual quality, than the available LDS, Fourier descriptor model, and higher-order SVD (HOSVD).

• Journal of the Korea Institute of Information Security & Cryptology
• /
• v.21 no.3
• /
• pp.183-188
• /
• 2011

According to increased multimedia data(i.e., MPEG video stream) in mobile application, protecting data becomes an important problem in the multimedia data delivery. SECMPEG is a selective encryption approach for protecting multimedia data. However, the computational overhead of SECMPEG's security level 3 is quite large because it encrypts the whole I-frames whose size is relatively larger than P/B-frames. Therefore, we need to analyze the characteristics of MPEG2 standard and derive an effective encryption of the I-frames. In this paper, we propose a slice-level, selective encryption approach by using the error-propagation characteristics of I-frames by a receiver. Our experimental results show that the proposed approach can reduce the execution time of SECMPEG's security level 3 by a factor of 30 without degradation of the security.

• Food Science and Biotechnology
• /
• v.18 no.2
• /
• pp.396-401
• /
• 2009

The nucleotide sequence contains 2 open reading frames encoding a 45-amino-acid protein homologous to a transcriptional repressor protein CopG, and a 203-amino-acid protein homologous to a replication protein RepB. Putative countertranscribed RNA, a double-strand origin, and a single-strand origin were also identified. A shuttle vector, pUCL2.1, for various lactic acid bacteria (LAB) was constructed on the basis of the pCL2.1 replicon, into which an erythromycin-resistance gene as a marker and Escherichia coli ColE1 replication origin were inserted. pUCL2.1 was introduced into E. coli, Lc. lactis, Lactobacillus (Lb.) plantarum, Lb. paraplantarum, and Leuconostoc mesenteroides. The recombinant LAB maintained traits of transformed plasmid in the absence of selection pressure over 40 generations. Therefore, pUCL2.1 could be used as an E. coli/LAB shuttle vector, which is an essential to engineer recombinant LAB strains that are useful for food fermentations.

• Journal of Microbiology and Biotechnology
• /
• v.33 no.2
• /
• pp.211-218
• /
• 2023

A cryptic plasmid (pTH32) was characterized from Tetragenococcus halophilus 32, an isolate from jeotgal, Korean traditional fermented seafood. pTH32 is 3,198 bp in size with G+C content of 35.84%, and contains 4 open reading frames (ORFs). orf1 and orf2 are 456 bp and 273 bp in size, respectively, and their translation products showed 65.16% and 69.35% similarities with RepB family plasmid replication initiators, respectively, suggesting the rolling-circle replication (RCR) mode of pTH32. orf3 and orf4 encodes putative hypothetical protein of 186 and 76 amino acids, respectively. A novel Tetragenococcus-Escherichia coli shuttle vector, pMJ32E (7.3 kb, Em^r), was constructed by ligation of pTH32 with pBluescript II KS(+) and an erythromycin resistance gene (ErmC). pMJ32E successfully replicated in Enterococcus faecalis 29212 and T. halophilus 31 but not in other LAB species. A pepA gene, encoding aminopeptidase A (PepA) from T. halophilus CY54, was successfully expressed in T. halophilus 31 using pMJ32E. The transformant (TF) showed higher PepA activity (49.8 U/mg protein) than T. halophilus 31 cell (control). When T. halophilus 31 TF was subculturd in MRS broth without antibiotic at 48 h intervals, 53.8% of cells retained pMJ32E after 96 h, and only 2.4% of cells retained pMJ32E after 14 days, supporting the RCR mode of pTH32. pMJ32E could be useful for the genetic engineering of Tetragenococcus and Enterococcus species.

• Journal of Microbiology and Biotechnology
• /
• v.14 no.3
• /
• pp.593-598
• /
• 2004

The arsenical resistance (ars) operon was cloned from a 67-kilobase pair (kb) plasmid, which was previously shown to be responsible for arsenic salts resistance in K. oxytoca D12. When plasmid pAE48, carrying the ars operon, was transformed into E. coli, transformed cells displayed enhanced survival in the presence of 4 mM arsenite, 50 mM arsenate, or 0.4 mM antimonite. The nucleotide sequence of the 5.6-kb fragment encoding arsenical resistance revealed five open reading frames (ORFs), which were predicted to encode polypeptides of 12.8 (arsR), 13.4 (arsD), 62.6 (arsA), 45 (arsB), and 16.7 (arse) kilodaltons (kDa). Each ORF was preceded by a ribosome binding site. A putative promoter-like sequence was identified upstream of arsR, and a possible termination site was found downstream of arsC. When the deduced amino acid sequences of the K. oxytoca Dl2 Ars proteins were compared with the amino acid sequences of the E. coli R773 Ars proteins, a significant amino acid similarity was observed (87.9％ for ArsR, 89.2％ for ArsD, 83.2％ for ArsA, 92.6％ for ArsB, and 91.3％ for ArsC), suggesting an evolutionary relationship of the ars genes of E. coli plasmid R773 and K. oxytoca Dl2.

• Journal of Microbiology
• /
• v.40 no.1
• /
• pp.43-50
• /
• 2002

A cluster of genes encoding the pyruvate dehydrogenase complex (PDC) of Streptomyces seoulensis, a Gram-positive bacterium, was cloned and sequenced. The genes of S. seoulensis consist of four open reading frames. The first gene, lpd, which encodes a lipoamide dehydrogenase, is followed by pdhB encoding a dihydrolipoamide acetyltransferase (E2p), pdhR, a regulatory gene, and pdhA encoding a pyruvate dehydrogenase component (Elp). Elp had an unusual homodimeric subunit, which has been known only in Gram-negative bacteria S. seoulensis E2p contains two lipoyl domains like those of humans and Streptomyces faecalis. The pdhR gene appears to be clustered with the structural genes of S. seoulensis PDC. The PdhR-overexpressed S. seoulensis howed growth retardation and the decrease of Elp, indicating that PdhR regulates the function of PDC by repressing the expression of Elp. A strain of Streptomyces licidans overexpressing S. seoulensis PdhR showed a significant decreasein the level of actinorhodin, implying a regulatory role for Streptomyces PDC in antibiotic biosynthesis.

• Korean Journal of Microbiology
• /
• v.52 no.1
• /
• pp.110-115
• /
• 2016

A small plasmid (pDK4) from the Antarctic marine organism Pseudoalteromonas sp. PAMC 21150, was purified, sequenced and analyzed. pDK4 was determined to be 3,480 bp in length with a G+C content of 41.64% and contains three open reading frames encoding a replication initiation protein (RepA), a conjugative mobilization protein (Mob) and a hypothetical protein. PCR-amplified pDK4 was cloned in high-copy pUC19 to yield the fusion vector pDOC153. The chloramphenicol resistance gene was inserted into pDOC153 to give an ampicillin and chloramphenicol-resistant, Pseudoalteromonas - Escherichia coli shuttle vector (7,216 bp; pDOC155). The TonB-dependent receptor (chi22718_IV ) and exochitinase (chi22718_III ) genes from Arctic marine P. issachenkonii PAMC 22718 were cloned into pDOC155 to produce pDOC158 and pDOC165, respectively. Both vector derivatives were transferred into plasmid-free Pseudoalteromonas sp. PAMC 22137 by the triparental mating method. PCR experiments showed that the genes were stably maintained both in Pseudoalteromonas sp. PAMC 22137 and E. coli $DH5{\alpha}$ cells, indicating the potential use of pDOC155 as a new gene transfer system into marine Pseudoalteromonas spp.

• Proceedings of the Korean Society of Plant Pathology Conference
• /
• 2003.10a
• /
• pp.94.2-95
• /
• 2003

E. intermedium 60-2G possessing a strong ability to solubilize insoluble phosphate, has plant growth-promoting activity, induced systemic resistance activity against scab pathogen in cucumber, and antifungal activity against various phytopathogenic fungi. The phosphate solubilizing activity of 60-2G may be mainly accomplished by production of gluconic acid through a direct extracellular oxidation of glucose by glucose dehydrogenase that required a PQQ cofactor for its activation. A pqq gene cluster conferred Phosphate-solubilizing activity in E. coli DH5${\alpha}$ was cloned and sequenced. The 6,783 bP pqq sequence had six open reading frames (from A to F) and showed 50-95％ homology to pqq genes from other bacteria. The E. coli strain expressing the pqq genes solubilized phosphate from hydroxyapatite after a pH drop to 4.0, which paralleled in time the secretion of gluconic acid. To study the role of PQQ in biocontrol traits of E. intermedium, PQQ mutants of 60-2G were constructed by marker exchangee mutagenesis. The PQQ mutants of E. intermedium were lost activities of solubilizing phosphate, growth inhibition of phytopathogenic fungi, and plant growth promotion. These findings suggest that PQQ plays an important role, possibly activation of certain enzymes, in several beneficial bacterial traits of E. intermedium by as yet an unknown mechanism.