• Journal of Ginseng Research
• /
• v.19 no.1
• /
• pp.51-55
• /
• 1995

The DNA fragment containing ginseng ribulose-1,5-bisphosphate carboxytase/oxygenase large subunit(rbcL) gene was cloned from the ginseng chloroplast EcoRl library by colony lift hybridization with tobacco rbcL gene probe. From the screened clone, the DNA fragment containing ginseng rbcL gene was digested with several restriction enzyme and analyzed by Southern blot hybridization for the construction of restriction map. The ginseng rbcL gene fragment was subcloned in pBluescript II SK + vector and sequence analysis was performed. The nucleotide sequence of ginseng rbcL gene was compared with those of petunia, tobacco, alfalfa, rice and barley, which showed a homology of 93.1%, 95.2%, 90.5%, 85.5% and 84.3%, respectively.

• Journal of Ginseng Research
• /
• v.19 no.3
• /
• pp.249-253
• /
• 1995

In the browning reaction of Korean ginseng, it appears that enzymatic and non-enzymatic browning reaction occurred in the initial stage of heating fresh ginseng at low temperature, and then non-enzymatic browning reaction followed in the drying period after heating. Activation energy of the browning reaction for red ginseng was about 9.0 kcal/mol. Browning reaction of red ginseng was accede- rated with an increase in steaming time, and a great extent of browning reaction occurred between 60-90 min of steaming at 10$0^{\circ}C$. Browning pigments of red ginseng were mostly water soluble subset.

• Korean journal of applied entomology
• /
• v.20 no.4 s.49
• /
• pp.223-227
• /
• 1981

These experiments were conducted to investigate the nematicidal effects of the insecticides, Mocap(O-Ethyl-S, S-dipropyl phosphorodithioate), Carbofuran(2,3-Dihydro-2,2-dimethyl benzofuranyl ethyl carbamate), Terbufos (S-tert-buthylthio methyl O,O-diethyl phosphordithioate) and their mixtures (Mocap+carbofuran, Mocap+Terbufos, Carbofuran+Terbufos) on growth of 2year-old ginseng, Panax ginseng C.A. Meyer, and the control of root-knot nematodes. There was no evidence of plant injury from insecticide treatment of ginseng, although the rate of emergence of the treated ginseng was slightly inhibited. The insecticide treatments showed no of-flavor of ginseng plant. Terbufos and Mocap provided heifer confrol of the root-knot nematodes than carbofuran alone and their mixtures. Mixtures of the insecticides showed antagonisitic effect to the root-knot nematodes.

• Journal of Ginseng Research
• /
• v.8 no.2
• /
• pp.105-113
• /
• 1984

This study was conducted to investigate chemical and physical characteristics of pectin in Korean ginseng plant. The results obtained are as follows: 1, The molecular weight of the pectin in ginseng plant was in the range of 1.1-2.4x 104 and athydrouronic acid content in the pectin was 97.98%. 2. The intrinsic viscosity of pectin as well as apparent visosity of the pectin in the roots were increased with cultural period. 3. The IR spectra of ginseng pectin showed the OH stretch, C-H bending vibration and the vibration of ester group carboxyl. 4. Sugars present in galacturonic acid, glucose, arbinose, xylose, galactose and rhamnose.

• Journal of Ginseng Research
• /
• v.19 no.3
• /
• pp.231-236
• /
• 1995

The antioxidant activity and the pharmacologically active constituents such as nucleosides and phenolic substancs of Panax ginseng C.A. Meyer (white ginseng powder) treated with gamma irradiation (Rl group) and ethylene oxide fumigation (EO group) were compared with those of non-treated ginseng (control group). High performance liquid chromatograms of nucleosides in three groups were similar with each other. The bathochromic effect of phenolic substances at W absorption region tends to be higher for EO group than Rl group. This tendency was also observed in heat treatment of each group. However, the antioxidant activity may have some effects on the labile property of phenolic substances. In summary the gamma-irradiated ginseng was more stable than the ethylene oxide-treated ginseng, but both ginseng samples gave the similar antioxidant activity.

• Journal of Plant Biology
• /
• v.35 no.1
• /
• pp.45-51
• /
• 1992

The changes of protein bodies in endosperm cells of both seeds with red seed coat and indehiscent seeds of Panax ginseng C.A. Meyer have been investigated in relation to the embryo development. In the early stage of seeds with red seed coat, spherical spherosomes were distributed in endosperm cells. Protein bodies were formed from vacuoles containing the storage protein. Cell organelles were hardly observed in the cytoplasm. In the late stage of the seed with red seed coat, the endosperm was filled with spherosomes and protein bodies. The protein bodies consisted of amorphous inclusions with high electron density or proteinaceous matrix with even electron density. In the seed of in dehiscence, the protein body in endosperm cells contained globoids and protein crystalloids. The globoid of protein body had a electron dense materials. Umbiliform layer was formed between embryo and endosperm. The deformation patterns of endosperm cell wall and the cellulose microfibril were observed in endosperm cells near the umbiliform layer. Umbiliform layer consisted of lipid body and autolyzed cell debris. The protein body of endosperm cell near the umbiliform layer showed various degenerative patterns, and so electron density of proteinaceous matrix was gradually decreased.reased.

• Bulletin of Food Technology
• /
• v.18 no.2
• /
• pp.3-13
• /
• 2005

인삼의 효능 및 유효성분에 관한 수많은 문헌 및 연구사례가 보고 되어 있음에도 불구하고 인삼의 주성분인 ginsenosides의 합성경로나 그와 관련된 유전인자에 대하여서는 거의 밝혀져 있지 않다. 인삼의 생리적 특성규명 및 ginsenosides의 생합성 경로를 밝히기 위한 한 방법으로써 프로테옴 분석이 시도되었다. 본지에서는 고려인삼 (Panax ginseng C.A. Meyer)을 대상으로 이루어진 최근의 프로테옴 분석 및 이차 대사산물의 생산과 연관된 유전인자를 찾기 위하여 이루어진 인삼의 기능유전체 분석을 소개하고자 한다.

• Journal of Ginseng Research
• /
• v.14 no.3
• /
• pp.416-420
• /
• 1990

Ultrastructural and anatomical features of the leaf were studied in Panax ginseng C.A. Meyer(ginseng). The ginseng leaf poorly developed palisade tissue and the size of mesophyll cell was larger and the chloroplast density was lower than that of Glycine max (soyben). Ginseng chloroplast was filled with highly stacked grana and condensely-arrayed thylakoid, so the stroma space was hardly absorbed. However, ginseng mesophyll tissue and chloroplast array did not reduce light energy entering the mesophyll chloroplast, and the high LHCP/CP ratio of ginseng thylakoid resulted in the absorption of excess photon. It is reasonable to assume that 1O1-photogenearation by excess light energy partially resulted from the anatomical and ultrastructural characteristics of the ginseng leaf.

• Journal of Ginseng Research
• /
• v.13 no.2
• /
• pp.153-157
• /
• 1989

The characteristics of the areal parts, photosynthetic and respiration rates and stem color were examined in 1- to 5-year-old Green-stem, Violet-stem and Yellow-berry variant ginseng (Panax ginseng C.A. Meyer). The top characteristics, photosynthetic and respiration rates were not significantly different among the three variants. Selfed progency of the Green-stems variant plant produced only green stems, while the others produced green and violet stem in the seedling stage. These results showed that most of the top characteristics of the Green-stem variant were similar those of the Violet-stem and Yellow-berry variants with age.

• Journal of Ginseng Research
• /
• v.14 no.2
• /
• pp.135-141
• /
• 1990

In order to elucidate the mechanism of the leaf-burning disease of ginseng (Panax ginseng C.A. Meyer), the relationships between thylakoid membrane peroxidation and chlorophyll bleaching were investigated in comparison with the ones of soybean (Glycine max L). When I measured the rate of lipid peroxidation in the thylakoids of ginseng and soybean by irradiation of light(60 w.m-2), it was identified that, the remarkably lower rate of lipid peroxidation was found in the ginseng thylakoid than the case of soybean. When lipid peroxidation of ginseng thylakoid was induced in the dark, chlorophyll contents of thylakoid was not changed. The results suggest that lipid peroxidation does not affect the chlorophyll bleaching in ginseng thylakoid. Thylakoid membrane peroxidation as well as chlorophyll bleaching was closely related with photosynthetic electron transport. But, according to the quenching experiment active oxygen species induced lipid peroxidation may be different species in the case of chlorophyll bleaching.