• Journal of Plant Biology
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• v.26 no.4
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• pp.191-196
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• 1983

The isolatin and culture of protoplasts from hypocotyl originated callus of Phaseolus vulgaris cv. Damyang were carried out. The maximum protoplast yield of 4.6$\times$105 per gram fresh callus, using the 13-day-old callus, was obtained by digeston for 6 hours in the enzyme solution. After 10 day-culture of the isolated callus protoplsts, plating efficiency was 50%. Thereafter, cell cluster medium, and followed by leading to callus formation on an agar medium after 3 weeks of the liquid culture.

• Journal of Plant Biology
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• v.27 no.3
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• pp.173-178
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• 1984

Callus culture of Phaseolus vulgaris L. was carried out to examine the ability to grow on cytokinin-free medium. Of the sixteen cultivars of P. vulgaris, eight were classified as completely cytokinin-autonomous phenotype and five were found to be cytokinin-dependent phenotype. Intermediate phenotype was shown in three cultivars. Using cv. Palgong and ca 21 as cytokinin-dependent genotypes, the genotype responses to the cytokinin requirements of callus tissue were studied in detail. The callus tissue of cv. Palgong and ca 21 were never habituated in cytokinin-free medium, regardless tissue origin and cytokinin concentration in previous passages. The result suggests that cytokinin dependency of callus tissue of P. vulgaris cv. Palgong and ca 21 may be due to inactivation of cytokinin biosynthetic pathway.

• Bulletin of the Korean Chemical Society
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• v.25 no.7
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• pp.955-956
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• 2004

• Bulletin of the Korean Chemical Society
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• v.30 no.10
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• pp.2422-2424
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• 2009

• Bulletin of the Korean Chemical Society
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• v.28 no.2
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• pp.175-176
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• 2007

• Korean Journal of Food Science and Technology
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• v.33 no.1
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• pp.113-116
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• 2001

The effects of phaseolus vulgaris extracts on serum lipid concentrations were evaluated in rats. Forty-two male Sprague-Dawley rats weighing $100{\pm}10\;g$ were divided into six groups and fed high fat diets for four weeks. Experimental groups were administered with following diets; basal fat diet(control) and basal and high fat diets with 1% cholesterol and phaseolus vulgaris extract(40 mg/kg, 400 mg/kg and 4000 mg/kg). The concentrations of serum triglyceride in rats fed the phaseolus vulgaris extract, high fat, 1% cholesterol, and PVHC-40, -400, -4000 mg/kg were lower than those in other groups. The concentrations of total cholesterol in the PVHC-40, -400, -4000 mg/kg groups were lower than those in fat diet groups. The concentrations of HDL-cholesterol in serum of the PVHC-40, -400, 4000 mg/kg groups were significantly higer than those of other groups. The levels of LDL-cholesterol in serum of the PVHC-40, -400, -4000 mg/kg groups were tended to be lower than those of other groups. These results suggest that phaseolus vulgaris extract may reduce elevated levels of serum lipid concentrations in rats fed high fat diets.

• Journal of Forest and Environmental Science
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• v.24 no.2
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• pp.61-68
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• 2008

Acrylamide is present as a contaminant in heated food products, predominantly from the precursor asparagine. Nonanchored inter simple sequence repeats (ISSRs) are arbitrary multiloci markers produced by PCR amplification with a microsatellite primer. In order to assess the feasibility of microsatellite primers as markers for DNA damage, the study was conducted on common bean (Phaseolus vulgaris L.) exposed to different concentrations of acrylamide. Polymorphisms were abundant among plant samples treated with acrylamide in comparison to control (untreated one) tested with 4- tri-nucleotide, 2 tetra-nucleotide, and 3- dinucelotide primers. The primer (CCG)4 was the best tested primer to generate polymorphism between the DNA of plants treated or not by acrylamide. Polymorphisms became evident as the presence and absence of DNA fragments in treated samples compared with the untreated one. The highest number of DNA variation on ISSR patterns was observed at the micromollar concentrations of acrylamide. Acrylamide was able to induce DNA damage in non concentration-dependent manner with effectiveness at micromollar concentrations. This study demonstrated that ISSR markers can be highly reliable for identification of DNA damage induced by acrylamide.

• Journal of Plant Biology
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• v.32 no.4
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• pp.323-330
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• 1989

This work was carried out to elucidate effects of endogenous and exogenous IAA transport on adventitious root formation in Phaseolus vulgaris hypocotyl cuttings. For inverted or normal incubation in distilled water, the adventitious root is always formed at the morphological base but not at the morphological apex. For inverted incubation, in both distilled water and certain chemical solution, the root formation is retarded more at the first stage (0-24 hr) than at the second stage (24-48 hr). When p-chlorophenoxyisobutyric acid (PCIB) was applied to the cuttings at the first stage, theroot formation was inhibited more than at the second stage. Treatment of 2,3,5-triiodobenzoic aicd (TIBA)markedly inhibited the adventitious root formation in Phaseolus vulgaris hypocotyl cuttings. This inhibition influenced the root according to the applied stage and period. Therefore, the root formation is more related to the stage of root primordium formation than to the stage of root elongation from the primordium. Inhibition of auxin transport oraction by TIBA or PCIB could also be reversed when hypocotyl cuttings are incubated in exogenously applied IAA solution.

• Food Science and Biotechnology
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• v.14 no.5
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• pp.672-675
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• 2005

Two anthocyanins were isolated from 1% HCl-20% methanol extracts of KG 97287 black common bean (Phaseolus vulgaris L.) using semipreparative, high-performance liquid chromatography (HPLC). The anthocyanins were identified using a combination of LC/ES-mass spectrometry (MS) and spectroscopic methods of UV-Vis, $^1H-$ and $^{13}C-$ nuclear magnetic resonance (NMR). The chemical structures of these two anthocyanins were elucidated as delphinidin 3-glucoside and petunidin 3-glucoside and their contents in KG 97287 black common bean seed coats were determined to be $2.614{\pm}0.11$ and $0.167{\pm}0.01\;mg/g$, respectively. These contents were lower than reported internationally and we recommend the introduction into Korea of high anthocyanin varieties of black common bean.

• Journal of Plant Biology
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• v.16 no.1_2
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• pp.12-16
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• 1973

The inhibitory effect of Inula sesquiterpene lactones on the growth of Phaseolus vulgaris was tested and the abnormality of the stem organization caused by the lactones was also examined. The longitudinal growth of the young stem and the expansion of the young leaf were stopped by the application of the lactones. However, this inhibitory effect was appeared and strictly restricted within the treated area. So the young shoot was observed for possible bending as a result of the unilateral application of the lactones. When the application of the lactones into the medium, the growth of the plant was entirely repressed. However, the growth of shoot and re-initiation of root were started after the plant was transfered to the lactone free medium. And partial reversal of inhibition of the stem growth was achieved by the additions of gibberelline and the lactones.