• Korean Journal of Poultry Science
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• v.27 no.3
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• pp.243-254
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• 2000

The effect of supplemental microbial phytase and non - phytate phosphorus(NPP) levels on layer productivity and nutrient digestibility were conducted in 640 21 weeks - old HyLine brown layer for 12 weeks. Supplemented phytase levels were 0, 300, 500 and 1,000 DPU/kg diet. NPP levels were adjusted with tricalcium phosphate(TCP), which were 0(0.11% NPP), 0.5(0.20), 1.0(0.29) and 1.5%(0.38). ME, CP and Ca levels were maintained at 2,800㎉/kg diet, 16% and 3.5%, respectively. Egg production was increased with phytase compared to without phytase(P〈0.05). Increasement of egg production was higher latter of experimental period. Egg production was not different to phytase levels. Egg production in TCP levels were increased in above 0.5% compared to 0% TCP. Difference of egg production by TCP was higher after 6 week. Especially, egg production to supplemental phytase was higher in 0% TCP. Egg weight was not different to phytase and TCP levels. Egg mass was increased with phytase compared to without phytase, but not difference significantly. There was similar to phytase levels. Egg mass in TCP group was increased in TCP supplementation(P〈0.05). Feed intake was not different in phytase levels, and greater with increasing TCP levels(P〈0.05). Feed conversion was improved with phytase(P〈0.05), and not difference in TCP levels. All of nutrients digestibility tended to improve with phytase, P(P〈0.05), especially. There were not different among phytase levels. The effect of adding phytase was higher in low phosphorus diets compared normal levels. Eggshell breaking strength and eggshell thickness also improved in added phytase(P〈0.05). Tibial ash and P content were slightly increased with phytase, and Ca content also was higher(P〈0.05) compared without phytase. We concluded that supplemental phytase in low phosphorus diet was showed to increase laying performance, feed efficiency, nutrients digestibility, egg quality, and bone development. Phytase supplementation was able to compensate for low NPP diet. We also thought optimum phytase level is 300 DPU, and can decrease NPP supplementation adding phytase in later diet.

• The Korean Journal of Mycology
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• v.27 no.4 s.91
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• pp.299-303
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• 1999

Phytase(myo-inositol-hexakis phosphate 3-phosphohydrolase, E C 3.1.3.8) sequentially hydrolyzes phytate to myo-inositol and inorganic phosphate. Phytase of Aspergillus ficuum was purified to homogeneity using ultrafiltration, cation exchange column and anion exchange column. It's molecular weight is estimated as around 90,000 by SDS-PAGE. Antibody against the phytase was produced by immunizing mice with the purified phytase. The titer of the antibody was determined to be 1/25,000.

• Journal of Animal Environmental Science
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• v.17 no.sup
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• pp.35-42
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• 2011

This work was conducted to investigate the effect of dietary phytase on growth performance and excreta excretion of growing-finishing pigs for 7 days. Eighty three crossbreeds (Yorkshire ${\times}$ Landrace ${\times}$ Duroc) of growing-finishing pigs were used in this work, and divided into 6 treatments. Six treatments were compared in a $2{\times}2$ factorial arrangement with 2 groups (5 replications/group, 8 head/replications) with the additive phytase and 3 groups(growing I, II and finishing phase) with growing phases. Initial weights with growing phases were $58.6{\pm}3.9$. $83.2{\pm}3.8$ and $111.4{\pm}5.4kg$, respectively. Body weight gain was high in phytase treatment (P<0.01) and low at finishing phase. Feed conversion ratio was high in no phytase treatment and at finishing phase (P<0.05). Feed and water intakes have no significant difference with phytase existence and growing phases. Feces excretion decreased with growing phase (P<0.05), and was low at phytase treatment (P<0.05). There was no significant difference on urine excretion (P>0.05). Nitrogen (N) and phosphorus (P) intake was not found significant difference with phytase and growing phases. N excretion had no significant difference on phytase existence and growing phase (P>0.05), but P excretion decreased at phytase treatment (P<0.05). N and P excretion ratio was low at phytase treatment (P<0.05). Finally, dietary phytase resulted in improvement of growth performance and reduction of excreta excretion of growing-finishing pigs.

• Korean Journal of Poultry Science
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• v.27 no.3
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• pp.169-179
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• 2000

An experiment was conducted to measure the effect of crude phytase supplementation on the growing performance, blood concentrations of some minerals and tibia characteristics of broiler chickens. Day-old 240 male broiler chickens (Avian) were randomly allotted to four treatments. There were six replicates per treatment, and ten chicks per replicate. Treatments consisted of two levels of crude phytase (0 and 600U/kg) made from Aspergillus ficuum and two levels of non-phytate P (0.45 and 0.35% NPP for the starter period, and 0.35 and 0.25% NPP for the grower period), making the experiment 2$\times$2 factorial. The starter period was from hatch to 21 d of age, and grower period was from 22 to 35 d of age. Feed intake and weight gain of chicks fed diet containing phytase were higher(P〈0.05) than those of chicks fed diets without phytase, however, no differences was found in feed/gain. mortality, and nutrient availabilities regarding the phytase supplementation. Chickens fed diets with low NPP and phytase excreted lower P than did birds fed diets containing normal NPP without phytase. The level of NPP and phytase did not affect N excretion. The Ca availability was increased by feeding low NPP diet. Dietary phytase increased the availabilities of P and Mg, but decreased those of Fe and Zn. There was interactions between dietary NPP level and phytase addition on mineral availability. Tibia was lighter and shorter in low NPP groups, and heavier in phytase treated groups. The tibial contents of Ca, P and Mg decreased in low NPP treated groups, but increased in phytase treated groups. The ash content of tibia of chickens fed diet with phytase was higher than that of birds fed diets without phytase. These data suggest that the crude phytase supplementation to broiler diets containing low NPP level improves growth performance and mineral availability and, reduces fecal P excretion.

• Journal of Life Science
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• v.7 no.2
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• pp.127-133
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• 1997

The phytase(myo-inositol hexkisphosphate phosphohydrolase ; EC 3.1.3.8) activity was observed only in the homogenate of intestinal mucosa, though the activity of alkaline phisphatase was measurable in various organs. In addition, no protein bands were detected in any other organs on immunoblotting using the anti-90kDa phytase antiserum. Thses results suggest that phytase is specifically present in small intestinal mucosa, and that hydrolysis of phytic acid(inositol-hexakisphosphate) can be allotted for a physiological role of the intestine-specific enzyme. The activities of phytase was increased during development of rat. The 70kDa phytase appeared just after birth, but the 90kDa phytase was not observed until adult period, suggesting that the 90kDa phytase was synthesized in response to weanling.

• Journal of Veterinary Clinics
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• v.18 no.1
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• pp.18-21
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• 2001

In this study, we assessed the efficacy of a novel B. amyloliquefacience DS11 phytase (DS11 phytase) and that of a commercial Aspergillus ficcum phytase (AF phytase) through their bioavailabilities of phytin-posphorus and -calcium in the diet using cannulated pigs. For the purpose of evaluating the efficacy of the phytases in pigs, we determined phosphorous concentrations from serum and feces, in addition to ingesta obtained from the cannula at the terminal ileum. As results, phosphorus concentration was lower in feces from DS11 group and BASF group by 17% and 10%, and higher in serum from the respective groups by 34% and 20%, as compared to the control group. Both phytases are evaluated to enhance phosphorus availability to the great extent. Calcium concentration of feces were lower in DS11 group and BASF group by 31% and 10%, than that in the control. Calcium concentration of serum was higher in DS11 phytase group by 4% but lower in AF phyase group by 3%, then that in the control group.

• Microbiology and Biotechnology Letters
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• v.18 no.4
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• pp.423-428
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• 1990

Three strains among 8 isolates from the fermented chungkookjang were shown the strong phytase productivities. The phytase activities in manufacturing chungkookjang with thrse bacteria were maximized after incubating at 35-$40^{\circ}C$, pH 7.0 for 5 day. The contents of same amino acids and riboflavin were increased in chungkookjang manufactured with these phytase-producing bacteria and the rate of phytic acid degradation was much higher in chungkookjang manufactured with a single or mixed cultures of these bacteria than in traditional chungkookjang.

• Applied Biological Chemistry
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• v.46 no.4
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• pp.291-298
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• 2003

A bacterial strain producing a high level of an extracellular phytase was isolated from livestock waste water, identified as a strain of Pseudomonas fragi and designated as Pseudomonas fragi Y9451. Under the phytase production medium, the activity of phytase reached the highest level after 120 hours of incubation. On the effect of carbon sources on the phytase production, the most favorable carbon source for phytase production was fructose. As for the effect of nitrogen sources, high levels of phytase activity were detected in the medium containing nutrient broth as the nitrogen source. Free $PO_4^{3-}$ inhibited phytase production with increasing concentration of $KE_2PO_4$ and phytate in the media. The addition of $CaCl_2$ and $MgSO_4$ also resulted in the inhibition of phytase production. To investigate the effect of aeration on the phytase production, different volumes of culture broth in Erlenmeyer flasks were incubated in rotary shaker at the speed of 200 rpm. As a result, a high level of phytase activity was detected at small volume of culture broth as compared to larger volume because of its more aerobic condition.

• Applied Biological Chemistry
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• v.48 no.3
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• pp.228-232
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• 2005

A yeast strain producing phytase, isolated from a mash of Korean traditional Yakju, was identified as a strain of Saccharomyces cerevisiae and designated as Saccharomyces cerevisiae CY strain. Phytase was produced by CY strain both intracellularly and extracellularly. Total phytase activity by the shaking culture was about two times higher than that of the static culture. The portion of extracellular phytase to total phytase activity ranged between 23 and 49 percent, depending on the glucose concentration in the culture medium. Phytase production was reached at approximately 1 U/ml as total phytase activity and the maximum intracellular phytase activity was 0.17-0.19 U/mg-DCW at late logarithmic growth phase. The optimum reaction pH and temperature of intracellular phytase were 3.5 and $40^{\circ}C$, respectively. Over 95% of the phytate was degraded by growing cells after 36 hours yeast cell culture and about 90% of total phytate was effectively degraded by suspending the whole cell with the biomass of 0.4 mg-DCW/ml-reaction solution after 12 hours degradation reaction.

• Animal Bioscience
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• v.34 no.3_spc
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• pp.463-470
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• 2021

Objective: This experiment was conducted to find out the immunological effects of wheat phytase when long-chain inorganic polyphosphate (polyP) treated with wheat phytase was added to a macrophage cell line, Raw 264.7, when compared to intact long-chain polyP. Methods: Nitric oxide (NO) production of Raw 264.7 cells exposed to P700, a long-chain polyP with an average of 1,150 phosphate residues, treated with or without wheat phytase, was measured by Griess method. Phagocytosis assay of P700 treated with or without phytase in Raw 264.7 cells was investigated using neutral red uptake. The secretion of tumor necrosis factor α (TNF-α) by Raw 264.7 cells with wheat phytase-treated P700 compared to intact P700 was observed by using Mouse TNF-α enzyme-linked immunosorbent assay kit. Results: P700 treated with wheat phytase effectively increased NO production of Raw 264.7 cells by 172% when compared with intact P700 at 12 h exposure. At 5 mM of P700 concentration, wheat phytase promoted NO production of macrophages most strongly. P700, treated with wheat phytase, stimulated phagocytosis in macrophages at 12 h exposure by about 1.7-fold compared to intact P700. In addition, P700 treated with wheat phytase effectively increased in vitro phagocytic activity of Raw 264.7 cells at a concentration above 5 mM when compared to intact P700. P700 dephosphorylated by wheat phytase increased the release of TNF-α from Raw 264.7 cells by 143% over that from intact P700 after 6 h exposure. At the concentration of 50 μM P700, wheat phytase increased the secretion of cytokine, TNF-α, by 124% over that from intact P700. Conclusion: In animal husbandry, wheat phytase can mitigate the long-chain polyP causing damage by improving the immune capabilities of macrophages in the host. Thus, wheat phytase has potential as an immunological modulator and future feed additive for regulating immune responses caused by inflammation induced by long-chain polyP from bacterial infection.