• Korean Journal of Agricultural Science
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• v.49 no.4
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• pp.821-831
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• 2022

American plum line pattern virus (APLPV), a member of the genus Ilarvirus in the family Bromoviridae, is one of the plant quarantine pathogens in Korea. In this study, 15 candidate primer sets were designed and examined to develop a reverse transcription polymerase chain reaction (RT-PCR) assay for plant quarantine inspection of APLPV. Using APLPV-infected and healthy samples, the primer sets were assessed for APLPV detection. To confirm the occurrence of nonspecific reactions, six ilarviruses (Apple mosaic virus, Asparagus virus 2, Blueberry shock virus, Prune dwarf virus, Prunus necrotic ringspot virus, and Tobacco streak virus) and 10 target plants (Prunus mume, P. yedoensis, P. persica, P. armeniaca, P. dulcis, P. tomentosa, P. avium, P. glandulosa, P. salicina, and P. cerasifera) were examined. Finally, two primer sets were selected. These primer sets could generate the expected amplicons even with at least 1 ng of the total RNA template in concentration-dependent amplifications. In addition, a positive clone was developed for use as a positive control in the abovementioned RT-PCR assay.

• Korean Journal of Agricultural Science
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• v.40 no.2
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• pp.93-99
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• 2013

The disease and insect were surveyed locally in greenhouse, fruit packing house and store house of 51 farms in 13 towns having purpose of paprika exportation. By analysis, various disease and insect were not only founded locally but more ones detected in farms having old facilities and no natural enemy. We found 15 pathogens such as Fusarium spp., Alternaria solani, Leveilluila taurica, PepMV (Pepino mosaic virus) and TMV (Tobacco mosaic virus) in greenhouse, Fusarium spp. in fruit packing house and Penicillium spp. in store house. We found 15 insects in greenhouse such as Bemisia tabaci, rialeurodes vaporariorum and Myzus persicae in greenhouse, Hylobitelus haroldi in fruit packing house. However, the problem quarantine disease and insect for importation and exportation were not detected in inspection time.

• Journal of Veterinary Science
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• v.19 no.6
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• pp.850-854
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• 2018

Novel H5N6 highly pathogenic avian influenza viruses (HPAIVs) were isolated from duck farms and migratory bird habitats in South Korea in November to December 2017. Genetic analysis demonstrated that at least two genotypes of H5N6 were generated through reassortment between clade 2.3.4.4 H5N8 HPAIVs and Eurasian low pathogenic avian influenza virus in migratory birds in late 2017, suggesting frequent reassortment of clade 2.3.4.4 H5 HPAIVs and highlighting the need for systematic surveillance in Eurasian breeding grounds.

• Korean Journal of Veterinary Research
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• v.62 no.2
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• pp.14.1-14.5
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• 2022

Salmonella enterica serotype Choleraesuis causes swine paratyphoid, with clinical findings of enterocolitis and septicemia. However, the clinicopathological features of S. Choleraesuis infections in pigs have not been reported in Korea. We describe the pathological findings of two weaned pigs with S. Choleraesuis infections, presenting with diarrhea, cough, and sudden death. Pathological examination indicated severe necrotic colitis in pig 1 and septicemic lesions in pig 2. Multidrug-resistant S. Choleraesuis was isolated from the pigs' lungs and intestinal contents. Further research is required for the surveillance of S. Choleraesuis infections in pigs and the virulence estimation in the S. Choleraesuis isolates.

• Korean journal of applied entomology
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• v.53 no.3
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• pp.301-303
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• 2014

The genus Charitoprepes (Warren), a probable vagrant group of the family Crambidae is newly recorded for the first time from the Korean Peninsula, which was described based on C. lubricosa (Warren) from Jeju islands. Diagnosis and illustrations of detailed diagnostic characters, including genitalia are provided.

• Mycobiology
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• v.33 no.4
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• pp.243-244
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• 2005

Three fungi newly intercepted from importing plants were identified in 2004. They were Ascochyta chrysanthemi on Lactuca sativa from China, A. spinaciicola on Spinacia oleracea from Denmark, and Leptosphaerulina australis on Brassica oleracea var. capitata from China. The characters of these fungi were described and illustrated.

• Korean Journal of Veterinary Research
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• v.53 no.4
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• pp.273-276
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• 2013

Two Korean black goat (approx. 2 and 3 years old) showing diarrhea and chronic weight loss were submitted to Animal and Plant Quarantine Agency. At necropsy, there were thickening of small intestine and enlargement of mesenteric lymph nodes. Microscopically, they had granulomatous enteritis in the small and large intestine and granulomatous lymphadenitis. By polymerase chain reaction (PCR) and acid fast stain, strong positive reaction and acid-fast rod bacteria were detected. According to the result of histopathology and PCR, we confirmed this case as Johne's disease. As far as we know, this is the first report of Johne's disease in Korean black goat.

• Korean Journal of Veterinary Research
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• v.54 no.4
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• pp.257-260
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• 2014

A calf suffering from diarrhea was admitted to the Animal and Plant Quarantine Agency for diagnostic evaluation. Postmortem examination revealed that the mesenteric lymph node was enlarged and small intestine wall was thin. Microscopically, a large number of small round organisms were attached to the small intestine villi. Villous atrophy and proprial neutrophil infiltration were also observed. Based on modified Ziehl-Neelsen staining, electron microscopy, and ELISA results, the calf was diagnosed with fatal cryptosporidiosis.

• Journal of Microbiology and Biotechnology
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• v.27 no.4
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• pp.868-868
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• 2017

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2004.10a
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• pp.42-43
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• 2004

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