• Journal of Microbiology and Biotechnology
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• v.11 no.2
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• pp.179-185
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• 2001

Nitrate grown cells of cyanobacterium Plectonema boryanum, transferred to nitrogen stress, evolved nitrogenase catalyzed $H_2$ under microaerophilic condition. Nitrogen ($N_2$) in gs phase, low light intensity, and reducing substances in incubation phase stimulated $N_2$fixation ($H_2\;evolution$). Cyanobacterium grew slowly under microaerobic condition with a low intracellular ammonia pool. Nitrogen sources (${NO_3}^-,{NH_4}^+,\;and\;CH_3NH_3$) inhibited nitrogenase and glutamine synthetase (GS) transferase activity, and methylamine behaved like an ammonical nitrogen source. Depletion of molybdenum (Mo) and addition of tungsten (W) in the incubation medium inhibited $H_2$ evolution, Cyanobacterium was able to take up nitrate and expressed nitrate reductase (NR) activity under microaerophilic condition at an extremely slow rate.