• Korean Journal of Medicinal Crop Science
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• v.21 no.4
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• pp.301-305
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• 2013

This study was performed to select adequate plant materials for developing a natural ${\alpha}$-glucosidase inhibitor by analyzing ${\alpha}$-glucosidase inhibition activity in fronds and rhizomes of three Dryopteridaceae species: Cyrtomium fortunei, Polystichum polyblepharum, and P. lepidocaulon. The highest ${\alpha}$-glucosidase inhibitor obtained from frond of P. lepidocaulon ($4.16{\mu}g{\cdot}mL^{-1}$), and rhizome of C. fortunei ($1.84{\mu}g{\cdot}mL^{-1}$), showed much higher inhibition activity than acarbose ($1413.70{\mu}g{\cdot}mL^{-1}$). The biomass required to inhibit ${\alpha}$-glucosidase by 50% was 0.04 ~ 0.35mg for frond and 0.03 ~ 0.10mg for rhizome, and P. lepidocaulon required the least amount of fronds and P. lepidocaulon the least rhizomes. In frond, ${\alpha}$-glucosidase inhibition activity was the highest in water fraction of C. fortunei ($20.2{\mu}g{\cdot}mL^{-1}$), and n-butanol fraction of P. lepidocaulon ($9.33{\mu}g{\cdot}mL^{-1}$) and P. polyblepharum ($5.10{\mu}g{\cdot}mL^{-1}$). In rhizome, it was the highest in n-butanol fractions of C. fortunei ($19.76{\mu}g{\cdot}mL^{-1}$) and P. polyblepharum ($4.47{\mu}g{\cdot}mL^{-1}$), and ethylacetate fraction of P. lepidocaulon ($5.46{\mu}g{\cdot}mL^{-1}$). The frond biomass required for 50% ${\alpha}$-glucosidase inhibition was the lowest in the water fraction of C. fortunei (1.43mg), and n-butanol fractions of P. lepidocaulon (1.10mg) and P. polyblepharum (0.66mg). The required biomass of rhizome was the lowest in the water fraction of C. fortunei (1.59mg), and n-hexane fractions of P. lepidocaulon (0.04mg) and P. polyblepharum (0.15mg). The result of this study suggested that the three Dryopteridaceae species had high ${\alpha}$-glucosidase inhibition activity with small biomass, which might have high value as materials for economical anti-diabetic medication.

• Korean Journal of Plant Resources
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• v.23 no.1
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• pp.38-46
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• 2010

Phenolic compound contents and scavenging activity on DPPH and ABTS radicals in twelve fern species were studied by analysing ultrasonification extraction of dried aerial parts and rhizomes using methanol solvent. Total polyphenol content ranged from 2.97 to 140.1 mg per 1 g dried sample and 0.80 to $49.58\;mg{\cdot}g^{-1}$ fresh sample. Highest polyphenol content was obtained with aerial part of Davallia mariesii and Polystichum lepidocaulon. Total flavonoid content of dried sample was $2.56{\sim}34.91\;mg{\cdot}g^{-1}$ and fresh sample $0.71{\sim}11.49\;mg{\cdot}g^{-1}$. Higher flavonoids were obtained with dried aerial part of Dryopteris crassirhizoma, but with fresh aerial part of P. lepidocaulon. In general, total polyphenol content was higher in rhizomes, except in case of total flavonoid. Scavenging effects on DPPH and ABTS radicals was higher with rhizome extracts compared to aerial parts. The rhizome of Polystichum polyblepharum showed highest effects on both radicals. However, the amount of fresh sample for $RC_{50}$ with consideration of water content and extraction yield. P. lepidocaulon aerial part was more advantageous than P. polyblepharum rhizome. All the fern extracts except for the Coniogramme japonica extract demonstrated superior scavenging effects on ABTS radicals, being similar activity of ascorbic acid and BHT.