• Title/Summary/Keyword: Pref-1

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Transcriptional activation of pref-1 by E2F1 in 3T3 L1 cells

  • Shen, Yan-Nan;Kim, Yoon-Mo;Yun, Cheol-Heui;Moon, Yang-Soo;Kim, Sang-Hoon
    • BMB Reports
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    • v.42 no.10
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    • pp.691-696
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    • 2009
  • The E2F gene family appears to regulate the proliferation and differentiation of events that are required for adipogenesis. Pref-1 is a transmembrane protein that inhibits adipocyte differentiation in 3T3-L1 cells. In this study, we found that the expression of pref-1 is regulated by the transcription factor E2F1. The expression of pref-1 and E2F1 was strongly induced in preadipocytes and at the late differentiation stage. Using luciferase reporter assay, ChIP assay and EMSA, we found that the -211/-194 region of the pref-1 promoter is essential for the binding of E2F1 as well as E2F1-dependent transcriptional activation. Knockdown of E2F1 reduced both pref-1 promoter activity and the level of pref-1 mRNA. Taken together, our data suggest that transcriptional activation of pref-1 is stimulated by E2F1 protein in adipocytes.

A study on Development Process of Fish Aquaculture in Japan - Case by Seabream Aquaculture - (일본 어류 양식업의 발전과정과 산지교체에 관한 연구 : 참돔양식업을 사례로)

  • 송정헌
    • The Journal of Fisheries Business Administration
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    • v.34 no.2
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    • pp.75-90
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    • 2003
  • When we think of fundamental problems of the aquaculture industry, there are several strict conditions, and consequently the aquaculture industry is forced to change. Fish aquaculture has a structural supply surplus in production, aggravation of fishing grounds, stagnant low price due to recent recession, and drastic change of distribution circumstances. It is requested for us to initiate discussion on such issue as “how fish aquaculture establishes its status in the coastal fishery\ulcorner, will fish aquaculture grow in the future\ulcorner, and if so “how it will be restructured\ulcorner” The above issues can be observed in the mariculture of yellow tail, sea scallop and eel. But there have not been studied concerning seabream even though the production is over 30% of the total production of fish aquaculture in resent and it occupied an important status in the fish aquaculture. The objectives of this study is to forecast the future movement of sea bream aquaculture. The first goal of the study is to contribute to managerial and economic studies on the aquaculture industry. The second goal is to identify the factors influencing the competition between production areas and to identify the mechanisms involved. This study will examine the competitive power in individual producing area, its behavior, and its compulsory factors based on case study. Producing areas will be categorized according to following parameters : distance to market and availability of transportation, natural environment, the time of formation of producing areas (leaderㆍfollower), major production items, scale of business and producing areas, degree of organization in production and sales. As a factor in shaping the production area of sea bream aquaculture, natural conditions especially the water temperature is very important. Sea bream shows more active feeding and faster growth in areas located where the water temperature does not go below 13∼14$^{\circ}C$ during the winter. Also fish aquaculture is constrained by the transporting distance. Aquacultured yellowtail is a mass-produced and a mass-distributed item. It is sold a unit of cage and transported by ship. On the other hand, sea bream is sold in small amount in markets and transported by truck; so, the transportation cost is higher than yellow tail. Aquacultured sea bream has different product characteristics due to transport distance. We need to study live fish and fresh fish markets separately. Live fish was the original product form of aquacultured sea bream. Transportation of live fish has more constraints than the transportation of fresh fish. Death rate and distance are highly correlated. In addition, loading capacity of live fish is less than fresh fish. In the case of a 10 ton truck, live fish can only be loaded up to 1.5 tons. But, fresh fish which can be placed in a box can be loaded up to 5 to 6 tons. Because of this characteristics, live fish requires closer location to consumption area than fresh fish. In the consumption markets, the size of fresh fish is mainly 0.8 to 2kg.Live fish usually goes through auction, and quality is graded. Main purchaser comes from many small-sized restaurants, so a relatively small farmer and distributer can sell it. Aquacultured sea bream has been transacted as a fresh fish in GMS ,since 1993 when the price plummeted. Economies of scale works in case of fresh fish. The characteristics of fresh fish is as follows : As a large scale demander, General Merchandise Stores are the main purchasers of sea bream and the size of the fish is around 1.3kg. It mainly goes through negotiation. Aquacultured sea bream has been established as a representative food in General Merchandise Stores. GMS require stable and mass supply, consistent size, and low price. And Distribution of fresh fish is undertook by the large scale distributers, which can satisfy requirements of GMS. The market share in Tokyo Central Wholesale Market shows Mie Pref. is dominating in live fish. And Ehime Pref. is dominating in fresh fish. Ehime Pref. showed remarkable growth in 1990s. At present, the dealings of live fish is decreasing. However, the dealings of fresh fish is increasing in Tokyo Central Wholesale Market. The price of live fish is decreasing more than one of fresh fish. Even though Ehime Pref. has an ideal natural environment for sea bream aquaculture, its entry into sea bream aquaculture was late, because it was located at a further distance to consumers than the competing producing areas. However, Ehime Pref. became the number one producing areas through the sales of fresh fish in the 1990s. The production volume is almost 3 times the production volume of Mie Pref. which is the number two production area. More conversion from yellow tail aquaculture to sea bream aquaculture is taking place in Ehime Pref., because Kagosima Pref. has a better natural environment for yellow tail aquaculture. Transportation is worse than Mie Pref., but this region as a far-flung producing area makes up by increasing the business scale. Ehime Pref. increases the market share for fresh fish by creating demand from GMS. Ehime Pref. has developed market strategies such as a quick return at a small profit, a stable and mass supply and standardization in size. Ehime Pref. increases the market power by the capital of a large scale commission agent. Secondly Mie Pref. is close to markets and composed of small scale farmers. Mie Pref. switched to sea bream aquaculture early, because of the price decrease in aquacultured yellou tail and natural environmental problems. Mie Pref. had not changed until 1993 when the price of the sea bream plummeted. Because it had better natural environment and transportation. Mie Pref. has a suitable water temperature range required for sea bream aquaculture. However, the price of live sea bream continued to decline due to excessive production and economic recession. As a consequence, small scale farmers are faced with a market price below the average production cost in 1993. In such kind of situation, the small-sized and inefficient manager in Mie Pref. was obliged to withdraw from sea bream aquaculture. Kumamoto Pref. is located further from market sites and has an unsuitable nature environmental condition required for sea bream aquaculture. Although Kumamoto Pref. is trying to convert to the puffer fish aquaculture which requires different rearing techniques, aquaculture technique for puffer fish is not established yet.

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Test-particle Solutions for Electron Acceleration in Low Mach Number Shocks

  • Kang, Hyesung
    • The Bulletin of The Korean Astronomical Society
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    • v.45 no.1
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    • pp.52.1-52.1
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    • 2020
  • We propose semi-analytic models for the electron momentum distribution in weak shocks that accounts for both in situ acceleration and reacceleration through diffusive shock acceleration (DSA). In the former case, a small fraction of incoming electrons is assumed to be reflected at the shock ramp and pre-accelerated to the so-called injection momentum, pinj, above which particles can diffuse across the shock transition and participate in the DSA process. This leads to the DSA power-law distribution extending from the smallest momentum of reflected electrons, pref, all the way to the cutoff momentum, peq, constrained by radiative cooling. In the latter case, fossil electrons, specified by a power-law spectrum with a cutoff, are assumed to be re-accelerated from pref up to peq via DSA. We show that, in the in situ acceleration model, the amplitude of radio synchrotron emission depends strongly on the shock Mach number, whereas it varies rather weakly in the re-acceleration model.

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A Study on the Plan Change in Japan Rural House by the Modernization - Focused on the Miyashiro in Saitama pref., Japan - (일본 농촌주택의 현대화에 의한 평면변화에 관한 연구 - 일본(日本) 기옥현 궁대정(宮代町)을 중심으로 -)

  • Kim, Kang Sub
    • Journal of the Korean Institute of Rural Architecture
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    • v.8 no.3
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    • pp.1-8
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    • 2006
  • A house is basic unit of human being living space and it reflects an occupation, family relation, a life style and sense of value of resident. To analyze of modernization process the rural house in Japan diversely and systematically, this study examined the elements and characteristics of changing floor plan in house through field studies and residential interviewing about the rural house of Miyashiro, Saitama pref. in Japan. The results of this study are as follow. First, the traditional TANOJI type changed into NAKAROUKA and TSUZUKIMA type on modernizing process. Second, Toma is an important space of farmhouse. It is succeeded with the element, which is the possibility of knowing the remnant of traditional element from modern rural house. Third, the cause of changing floor plan is a narrow and small space by growth of children. That is the most factor of changing house deterioration of equipments and necessity of children's space.

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Estimation of environmental damage assessment in the shoreline after the NAKHODKA oil-spill using Geo-informatics

  • Kim, Sang-Woo;Goto, Shintaro;Matui, Kouji;Shikada, Masaaki;Shikida, Asami;Sawano, Nobuhiro
    • Proceedings of the KSRS Conference
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    • 1999.11a
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    • pp.443-449
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    • 1999
  • The investigation of the amount of the ecosystem damage on the shoreline due to the NAHODKA oil-spill accident, which occurred in the Sea of Japan, was attempted by using geoinformatics. At first, it was assumed that symbolical vegetation's distribution could be specified in the coast in Ishikawa Pref. where the heavy oil was washed, and surveyed the regional distribution. Then, the presumption result of those environmental capacities was arranged by GIS. In addition, the amount of the ecosystem damage was presumed as cost necessary though a symbolical living thing for the retreat because of the base line by the heavy oil drifting ashore was recovered. By comparing the vegetation line and the surveying data which shows environmental capacity, the retreat areas of the vegetation were 1100-1200 $m^2$. When the amount of damage on the ecosystem of the NAHODKA oil-spill accident was presumed based on the retreat area of this vegetation and the restoration cost, the amount of damage within Shioya beach which 150m in the surveying range became 2 to 2.5 million Yen. Because the extension distance from the Shioya beach to the Katano beach was about 3,500m, the amount of damage became about 46 to 65 million Yen. As a result of calculation for the amount of damage on the ecosystem of the NAHODKA oil-spill accident, it was estimated approximately 1,400 to 2,000 million Yen in the shoreline of Ishikawa Pref., because the total extension of beaches in Ishikawa Pref. is about 110km.

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A comparative study of the effect of root planing methods and various conditioning agents (치근면 활택 방법 및 수종의 치근면 처리 약제의 효과에 대한 비교 연구)

  • Kang, Seung-Mo;Hong, Ki-Seok;Lim, Sung-Bin;Chung, Chin-Hyung
    • Journal of Periodontal and Implant Science
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    • v.35 no.3
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    • pp.719-730
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    • 2005
  • The purpose of this study was to compare the effect of tetracycline HCL, Citric acid and PrefGel applied on the root surfaces that planed with periodontal curret with Roto bur. In this study, 20 extracted teeth with advanced periodontal disease were used. The teeth were root planing with periodontal curette and Roto bur. Following root planing, each agents was burnished on the prepared root surface for 3 minutes to find opened dentinal tubules. And then, each specimens were investigated using scanning electron microscope. Amount of remained cementum by loss of tooth substance index and the number of opened dentinal tubules were evaluated to each specimens The results were as follows. 1. Groups treated with periodontal curette were almost seemed no removed. Other groups treated with Roto bur showed partially opened dentinal tubule orifices. 2. Loss of tooth substance index were compared between groups. There was no statistically difference between periodontal curette groups. Between Roto bur groups was alike. But there were statistically differences between periodontal curette and Roto bur groups. 3. At comparing with various root conditioning agents, Tetracycline HCL group took statistically higher than Citric acid and PrefGel in opened dentinal tubules. On the other hand, there was no statistically difference between Citric acid group and PrefGel group. As a result of this study, groups treated with Roto bur showed more cementum removed than groups treated with periodontal curette. In a treatment for regeneration of periodontal tissue, it was regarded that Roto bur should be used and that Tetracycline HCL would be more effective as chemical root conditioning agent.

Orientation and Microstructure of Watts and Bright Nickel Electrodeposits (Watts 니켈전착층과 광택니켈 전착층의 방위와 현미경조직)

  • 이동녕;예길촌
    • Journal of the Korean institute of surface engineering
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    • v.15 no.1
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    • pp.18-23
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    • 1982
  • The microstructure of Watts and bright ni-ckel electrodepositss is correlated with pref-erred orientation. The characteristics lamellar structure of bright nickel was atributed to the periodic adsorption of organic additives and related to cyclic fluctuations in temper-ature.

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A Study on the Planning and Architectural Characteristics of Regional Public Facilities through Resident Participation - Focused on the New City Hall of Miyashiro in Saitama pref., Japan - (주민 참여에 의한 지역공공시설 계획과 건축적 특성에 관한 연구 -일본(日本) 기옥현 궁대정(宮代町) 신청사(新廳舍)를 대상으로 -)

  • Kim, Kang Sub;Son, Kwang Ho;Lee, Sang Jung
    • Journal of the Korean Institute of Rural Architecture
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    • v.8 no.1
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    • pp.25-32
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    • 2006
  • The object of this study is the new city hall of Miyashiro in Saitama pref., Japan, which was built through local resident participation. The purpose of this study is to offer the reference data of regional public facilities by researching the process, role and meaning of resident participation and architectural characteristics and meanings. The results of the study are as follows. First, the new city hall of Miyashiro was built through voluntary will and efforts of the residents. Through close cooperation of the local residents, administration and specialists, it took into significant consideration regional conditions and individual characteristics. Second, the new city hall of Miyashiro applied the concept of barrier-free for children, the aged and handicapped, etc. Third, the new city hall of Miyashiro introduced wooden structure making the most of the merits of lumber. Finally, the new town hall of Miyashiro took sustainability into consideration by striving for the efficiency of energy application.

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Taxonomic Review of Pristiophorus japonicus Complex (Pristiophoridae, Chondrichthyes) in the Northwest Pacific (북서태평양 톱상어(톱상어과, 연골어강)의 분류학적 재검토)

  • Myoung, Se Hun;Kim, Jin-Koo;Song, Choon Bok;Kai, Yoshiaki
    • Journal of Marine Life Science
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    • v.1 no.1
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    • pp.8-17
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    • 2016
  • The family Pristiophoridae belonging to the order Pristiophoriformes consists of about 8 species and 2 genera in the world. Among them, only one species (Pristiophorus japonicus) is distributed in northwestern Pacific. The family is characterized by having an elongated and flattened rostrum with laterally projecting a low of sharp teeth. In order to clarify variability of local populations of P. japonicus, we investigated 16 individuals collected from four locations (Korea, n=6; Miyagi, n=1; Kochi Pref., n=1; Okinawa, n=8) for morphological and molecular analyses. Morphological analysis showed that there may exist three morphotypes, of which A morphotype (Korea, Miyagi, Kochi Pref.) has a short snout (26.8% of TL), being most similar to syntypes of P. japonicus. On the other hand, B morphotype (Okinawa) has a long (31.7% of TL) and wider snout (snout width at nostrils 5.2 times pre-oral length) and, C morphotype (Okinawa) has a long (31.7% of TL) and narrower snout (6.3 times). Molecular analysis using mitochondrial DNA cytochrome b DNA sequences (386 base pairs) showed that the K2P distance between A and C morphotypes were 2.1~2.7%, but those within A morphotype were 0.0~0.5%. We identified A morphotype as P. japonicus, and designated B and C morphotypes as Pristiophorus sp. 1 and Pristiophorus sp. 2, respectively. Further studies are required to elucidate the taxonomic status of two morphotypes of the sawshark in Okinawa.