• Journal of Veterinary Clinics
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• v.19 no.2
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• pp.147-152
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• 2002

Ovaries from total 192 slaughtered cows, 154 Korean native cows and 38 dairy cows were collected during the slaughtering process in Kimhae, Changyoung and Yangsan abattoirs in Kyungnam province from January 2001 to January 2002. Rates of pregnant and non-pregnant and ovarian findings were invested. Rates of pregnant cows in 192 slaughtered cows were 12.5% (24 cows) and in difference of cow breeds, 11.0% (17 cows) in 154 Korean native cows and 18.4% (7 cows) in 38 dairy cows from total 192 cows, respectively. Ages of fetuses in pregnant Korean native cows were mostly less than 4 months and ages of fetuses in dairy cows were mostly about 7-8 months. Cows which each diameter of follicles and corpus luteums in same cow was more than 5-6 mm in diameter were 69.8% (134 cows) in total 192 slaughtered cows and in difference of cow breeds, 64.7% (11 cows) in 17 Korean native cows and 57.1% (4 cows) in 7 dairy cows. Mean diameter of foliicles and corpus luteums in Korean native cows are 13.7$\pm$5.6$\times$ 11.2$\pm$4.6mm and 17.5$\pm$4.6$\times$14.6$\pm$4.0 mm in non-pregnat cows, and are 11.0$\pm$4.8$\times$9.1 $\pm$ 2.6mm and 21.2$\pm$2.9$\times$18.3$\pm$ 2.7 mm in pregnant cows, respectively. Mean diameter of follicles and corpus luteums in dairy cows are 15.8$\pm$7.1 $\times$ 14.3$\pm$ 6.0 mm and 20.3$\pm$5.9$\times$16.9$\pm$ 5.8 mm in non-pregnant cows, and are 10.1 $\pm$ 3.0$\times$9.2$\pm$2.3 mm and 23.0$\pm$ 1.7$\times$20.1 $\pm$ 1.3 mm in pregnant cows, respectivley. The above findings indicate that the co-appearance rate of follicles and corpus luteums in same cows are higher in both pregnant and non-pregnant cows. Compared in pregnant and non-pregnant cow ovaries, mean size of follicles are smaller in pregnant cows but size of corpus luteums are more larger in pregnant cows than in non-pregnant cows. Correlation of the follicle size (Y) and corpus luteum size (X) in same cows developed each other in inversive size. Those correlative formulas appeared to be Y = -0.2022X+17.175 in Korean native cows and Y= -0.5754 X+24.153 in dairy cows.

• Korean Journal of Pharmacognosy
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• v.28 no.3
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• pp.104-111
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• 1997

The traditional herbal medicine, Jackyakgamcho-tang(JGT), was reported to decrease serum testosterone levels and make pregnancy possibel in anovulatory woman and rat. JGT contains Paeoniae Radix(PR) and Glycyrrhizae Radix(GR) in equal amount. This study was designed to investigate the effect of JGT and its components(PR, GR, paeoniflorin and glycyrrhizin) on uterine and ovarian responses, follicular development, and estrogen secretion in the immature rat. The samples(water extracts of JGT, PR, GR; pure compound of paeoniflorin and glycyrrhizin) were administered orally to rats from the 21th day of age to the 28th or 30th days of age for 7 or 9 days. JGT(400mg/kg) and PR(100mg/kg, 200mg/kg) treatments significantly increased serum estradiol above levels in control rats, but both GR and glycyrrhizin had no effect on this parameter. Gross observation and histological analysis revealed that an increased number of growing follicules was observed in the ovaries of JGT and PR treated rat. However the lutenized follicles and ova present in the oviducts were not observed in all rats except one treated with estrogen as a positive control. These results indicate that JGT stimulates the estrogen production and follicular maturation in the immature rat and PR is the main component to induce such reaction.

• Journal of radiological science and technology
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• v.39 no.4
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• pp.549-555
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• 2016

This study was an evaluation of the significance of each parameter through aimed at pregnant women subjected to screening test(integrated test) in predicting risk of Down syndrome. We retrospectively analysed the correlation of risk of Down's syndrome with Nuchal Translucency(NT) images measured by ultrasound, Pregnancy Associated Plasma Protein A(PAPP-A), alpha-fetoprotein(AFP), unconjugated estriol(uE3), human chorionic gonadotrophin(hCG) and Inhibin A by maternal serum. As a result, a significant correlation with NT, uE3, hCG, Inhibin A is revealed with Down's syndrome risk(P<.001). In ROC analysis, AUC of Inhibin A is analysed as the biggest predictor of Down's syndrome(0.859). And the criterion for cut-off was inhibin A 1.4 MoM(sensitivity 81.8%, specificity 75.9%). In conclusion, Inhibin A was the most useful in parameters to predict Down's syndrome in the integrated test. If we make up for the weakness based on the cut-off value of parameters they will be able to be used as an independent indicator in the risk of Down's syndrome screening.

• The Korean Journal of Nuclear Medicine Technology
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• v.12 no.1
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• pp.62-65
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• 2008

Purpose: Vitamin $B_{12}$ and folic acid are type of water-soluble vitamin and those work as a secondary vital enzyme, but especially those are involved in the nuclear DNA synthesis. Those are mainly measured in order to diagnose megaloblastic anemia and to assess the storage of folic acid during pregnancy. It is generally known that the hemolyzed serum is useless for folic acid and vitamin $B_{12}$, but it is not easy to abide by this information because our lab (Samkwang Medical Laboratories) is reference laboratory. We tested how much the extent of hemolyzed serum had influence on the results. Materials and Method: We performed the tests of vitamin $B_{12}$, folic acid. For the hemolysis effect study, we used 40 cases. According to the level of A, B and C groups, each group had 10 samples which were mechanically hemolyzed serum. Results: Hemolysis did not affect the vitamin $B_{12}$ results. However in case of folic acid, the value increased according to the degree of hemolysis. And severe hemolyzed cases had the highest value (greater than 20 ng/ml). Conclusions: Preventing the hemolysis, it has to be informed that blood-collecting, separation and storage are performed more carefully. As you see from the above results, hemolyzed serum is not proper for folic acid test, and hemolysis does not affect the results of vitamin $B_{12}$. However, for the more accurate results, it is necessary to avoid hemolysis.

• Journal of The Korean Society of Grassland and Forage Science
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• v.35 no.1
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• pp.1-5
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• 2015

This study assessed the impacts of grazing and barn feeding on the reproductive performance of black goats and the growth performance of goatlings to gather basic data for the establishment of an ideal goat-breeding system. A total of 36 Korean black goats were grouped by feeding system (a pasture grazing group and a barn feeding group, n=18) over the 8-month study period from April to November 2011. The difference between the singleton pregnancy rate of the grazing group, measured at 31.5%, and the barn-feeding group's 37.0% was not statistically significant. Also, breeding type did not yield any meaningful differences, with 1.76 live births recorded for the grazing group and 1.69 recorded for the barn-feeding group. In regard to weaner goats, the grazing group showed a higher number compared to the barn-feeding group (p=0.11). The birth weight of the grazing group was measured at 2.3 kg, while that of the barn-feeding group was 2.29 kg, with breeding type yielding no significant differences. The grazing group's weight at 90 days of age was 9.97 kg, which was higher than the barn-feeding group's 9.45 kg (p=0.09). The grazing group showed higher daily weight gain during the lactation period than the barn-feeding group (p=0.13). Overall, with breeding type considered, the grazing group did not show significant differences in terms of the number of live births and birth weights compared to the barn-feeding group; however, the grazing group showed better outcomes in terms of daily weight gain. As it has been shown that grazing satisfies the physiological needs of goats more effectively than barn feeding, we believe that it contributes to the farming of healthy goats.

• Journal of Embryo Transfer
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• v.27 no.3
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• pp.133-139
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• 2012

This study was carried out to develop the useful inducing method of estrus for Korean native cows. Under the condition of estrus induction by administering $PGF_{2{\alpha}}$ for the cows in which corpus luteum (CL) in ovaries was detected by ultrasonography, ovarian responses and the changes of progesterone ($P_4$) concentration against $PGF_{2{\alpha}}$ compared with conception rate were observed in cows and heifers. In inducing estrus administering $PGF_{2{\alpha}}$. to the cows which has corpus luteum in ovaries, ovarian reponses, the changes of progesterone concentration, and conception rate were identified and compared. The results attained from the studies were as follows. Significant decreases of CL in size over time after $PGF_{2{\alpha}}$ administration were detected in both cow and heifer groups (p<0.001), but not different between groups in the CL regression rate (p>0.05). In addition, the percentage changes relative to the plasma $P_4$ concentration on day 1 after $PGF_{2{\alpha}}$ treatment were decreased to below 1ng/ml. The growth rate of follicle was observed as 31% on day 1 and 42% on day 2 in cows, and 34% on day 1 and 97% on day 2 in heifers, resulting that growth of heifers are faster than that of cows (p<0.05). The conception rate after $PGF_{2{\alpha}}$ treatment were 60.5% and 64.2% in cows and heifers, respectively. It also indicated that the conception rate after estrus observation with $PGF_{2{\alpha}}$ injection was as high as 66.6% while that with timed-artificial insemination (TAI) regardless of the estrus observation was 56%, which means the pregnancy rate of artificial insemination after estrus observation was higher than that of TAI (p<0.05). In the result of all above, there were significant decreases in CL size and the plasma $P_4$ concentration by days but rapid growth in follicles, which has no differences in cows and heifers. The conception rate was commonly high after estrus observation and more than 50% under TAI.

• Journal of Embryo Transfer
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• v.27 no.3
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• pp.141-147
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• 2012

This study was operated to establish induction using ultrasonography by estimating the relation of follicle size and estrus manifestation. Clinical estrus symptoms were observed 97.4% in cows and 87.5% in heifers when overall 55 cows were induced to estrus in a single dose of $PGF_{2{\alpha}}$ after verifying CL through ultrasonography, which means estrus hours among those 52 cows showing the clinical estrus symptoms were estimated 2.39 days on cows and for 2.37 days on heifers which showed no differences (p>0.05). The estrus manifestation hours according to the follicle size in cows didn't have any significance each other (p>0.05), though estrus hours was 54 hours (the shortest) with follicle size bigger than 10 mm and were made up within 69 hours. The estrus manifestation hours according to the follicle size in heifers didn't have any significance each other (p>0.05) and took around 42 hours (the shortest) with follicle size of 5mm (the smallest) and were made up within 66 hours. Follicles after $PGF_{2{\alpha}}$ injection were ovulated and assigned to many phases as follows; Group 1 (growing phase) - continuously growing into ovulation, Group 2 (growing and static phase) - delaying in growth after the growth of follicles, Group 3 (static and growing phase) - growing after growth delay, Group 4 (regressing and new growing phase) - the follicle is closed and a new follicle grows. In addition, the process of follicle development and estrus hours had no significance each other (p>0.05), though estrus manifestation hours in Group 1 and 2 was relatively short, and in Group 3 and 4 for a relatively long time. In the result of all above, the estrus manifestation hours after $PGF_{2{\alpha}}$ injection has no differences accoring to the follicle size in cows and heifers. Therefore, High pregnancy rate is obtained when practicing artificial insemination within 3 days in estrus or TAI in 72 to 80 hours after adminitrating $PGF_{2{\alpha}}$.

• Journal of Embryo Transfer
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• v.28 no.1
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• pp.25-30
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• 2013

The aim of this study was to estimate of ovulation time and parturition day at the same time as breeding in small dog by vaginal cytology and to confirm the accuracy by comparing the expected parturition day and the real one. Characteristic features of vaginal cytology during the estrous cycle were the high proportion of large intermediate cell, superficial cell, anuclear cell and erythrocyte in proestrus, superficial cell and anuclear cell in estrus, parabasal cell, small intermediate cell, large intermediate cell and leukocyte in diestrus, parabasal cell and small intermediate in anestrus, respectively. When day 0 was the parturition day, the period of pregnancy is 67.45(64~75) days when the cornification index (CI) is over 90%. Also, on the basis of ovulation day, 63.65(59~66) days was confirmed, and 57.0(52~60) days was confirmed based on the first day of diestrus. There are the gap of 4 days between the day being over 90% in CI and ovulation day. On the basis of this, when expecting parturition day based on the day being over 90% in CI by vaginal cytology, 18.1% was produced in the same of the expected parturition day and the real one, 30.3% and 33.3% were produced in the gap of one day and two days, respectively so, the accuracy within two days was 81.7%. In addition, based on the first day of diestrus, it also was identified to 81.7% as the difference between the expected parturition day and the real one within 2 days. It demonstrated there are any difference between any expected parturition day by vaginal cytology. Thus on the basis of the day of being over 90% CI, it is fully thought to using clinically due to the possibility of prediction the parturition day at the same time as the determination of the proper time of the optimal mating time.

• Fire Science and Engineering
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• v.27 no.5
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• pp.75-79
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• 2013

Application of the left lateral tilt position has been recommended during cardiopulmonary resuscitation (CPR) of pregnant patients. However, the left lateral tilt could displace the left ventricle (LV) besides the gravid uterus and may compromise the cardiac pump mechanism of CPR. Thus, we investigated the effect of left lateral tilt on the spatial relationship between the anterior-posterior axis (AP axis), which represents the direction of sternal displacement during CPR, and the LV. We retrospectively reviewed the medical records and multidetector computed tomography (MDCT) scans of 90 patients who underwent virtual gastroscopy using MDCT. Virtual gastroscopy was performed with the patient both in the left lateral tilt position and in the supine position. On an axial image showing the maximal area of the LV, the angle between the AP axis and the LV axis ($Angle_{AP-LV}$), the shortest distance between the AP axis and the mid-point of LV cavity ($D_{AP-MidLV}$) and the shortest distance between the AP axis and the LV apex ($D_{AP-Apex}$) were measured. In the supine scans, the LV was situated on the left side of the AP axis in 87 patients (96.7%). On the left lateral tilt scans, the mean tilt angle was $43.4{\pm}11.0^{\circ}$. $D_{AP-MidLV}$ and $D_{AP-Apex}$ were significantly longer in the left lateral tilt position (p<0.001), but $Angle_{AP-LV}$ was comparable between the positions. This study indicates that the left lateral tilt position may compromise the cardiac pump mechanism of chest compression in pregnant cardiac arrest patients.

• Journal of Embryo Transfer
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• v.20 no.3
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• pp.297-302
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• 2005

Artificial insemination (AI) technology has widely applied in domestic animals including cattle for production and preservation of valuable animals. However, AI in goat was poorly studied. We here compared two different estrus synchronization methods, CIDR (controlled intravaginal drug release)+$PGF_2a$ and CIDR+PMSG, in Korean native goats. After treatment of CIDR+$PGF_2a$ or CIDR+PMSG for estrus synchronization, AI was applied to 38 recipients. Of 18 recipients treated with CIDR+$PGF_2a$, 4 recipients produced offsprings. However, no pregnancy was obtained in 20 recipients treated with CIDR+PMSG. Especially, after treatment of CIDR+$PGF_2a$, AI with fresh or frozen sperm showed markedly higher parturition rates than that of CIDR+PMSG. From these results, we suggest that combination of CIDR and $PGF_2a$ could be used as good method for estrus synchronization in Korean native goats.