• Title/Summary/Keyword: Protoplast fusion and nuclear transfer

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Studies on Development of New Basidiomycetes by Protoplast Fusion and Nuclear Transfer I - The Antitumor Components of the Protoplast fusants - (원형질체 융합 및 핵전이에 의한 새로운 담자균류의 개발에 관한 연구(I) - 융합균사체의 항암성분 -)

  • Moon, Chul;Yoon, Jong-Myung;Kim, Chae-Kyun;Kim, Ha-Won;Choi, Eung-Chil;Kim, Byong-Kak
    • Korean Journal of Pharmacognosy
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    • v.27 no.3
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    • pp.224-230
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    • 1996
  • To find pharmacologically active hybrids among the inter-order protoplast fusants of Lentinula edodes and Ginoderma lucidam the antitumor test was performed and the fusant P22 was selected among them. The hot water and alkaline extracts from the cultured mycelia of P22 were purified and separated into four fractions by DEAE-cellulose anion exchange chromatography. When a dose of 20 mg/kg/day of each fractions was injected into ICR mice by i.p., the tumor inhibition ratio of Fr. IV against solid sarcoma 180 was the higher than any other fraction. Fr. IV was a protein-bound polysaccharide which was composed of 69. 12% polysacchafide and 9.76% protein and the molecular weight of Fr. IV was $6.7{\times}10^4$ dalton.

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Studies on Development of New Basidiomycetes by Protoplast Fusion and Nuclear Transfer II - The Effects of the Components of the Protoplast Fusants on Mouse Immune Cells - (원형질체 융합 및 핵전이에 의한 새로운 담자균류의 개발에 관한 연구(II) - 융합균사체의 항암성분이 생쥐의 면역세포에 미치는 영향 -)

  • Moon, Chul;Kim, Chae-Kyun;Yoon, Jong-Myung;Shim, Mi-Ja;Kim, Ha-Won;Choi, Eung-Chil;Kim, Byong-Kak
    • Korean Journal of Pharmacognosy
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    • v.27 no.3
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    • pp.231-237
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    • 1996
  • The antitumor components of the protoplast fusants of Lentinula edodes and Ganoderma lucidum were examined for immunological activity to elucidate the mechanism of their antitumor activity. They did not show any direct cytotoxicity against tumor cells. But being examined for immunopotentiation activity, they increased the number of colonies in the bone marrow stem cells to 3.0 times. They also increased the activities of the acid phosphatase in activated macrophages to 2.1 times and the secretion of nitric oxide in RAW 264.7 to 2.2 times, respectively. They activated the components of the alternative complement pathway. In humoral immunity. they increased the activities of the alkaline phosphatase in differentiated B cells to 1.6 times and the number of plaque forming cells to 1.8 times, respectively. In cellular immunity, they restored the depressed response of delayed type hypersensitivity in tumor bearing mice to normal level.

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