• Title/Summary/Keyword: Pseudomonas syringae

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Development and Evaluation of PCR-Based Detection for Pseudomonas syrinage pv. tomato in Tomato Seeds (토마토 종자로부터 PCR을 이용한 Pseudomonas syringae pv. tomato의 검출)

  • Cho, Jung-Hee;Yim, Kyu-Ock;Lee, Hyok-In;Yea, Mi-Chi;Cha, Jae-Soon
    • Research in Plant Disease
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    • v.17 no.3
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    • pp.376-380
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    • 2011
  • The bacterial speck of tomato caused by Pseudomonas syringae pv. tomato leads to serious economic losses especially on fruits of susceptible genotype. Thus, Pseudomonas syringae pv. tomato is a plant quarantine bacterium in many countries including Korea. In this study, we developed specific PCR assays for detection of the bacterium from tomato seeds. A specific primer set is designed from the hrpZ gene for specific detection of Pseudomonas syringae pv. tomato. A 501 bp PCR product corresponding to hrpZ gene was amplified only form Pseudomonas syringae pv. tomato strains, but no PCR product was amplified from other tomato bacterial pathogens, such as Pseudomonas syringae pv. glycinea, P. syringae pv. maculicola, P. syringae pv. atropurpurea, P. syringae pv. morsprunorum, and from other P. syringae pathovar strains. The nested-PCR primer set corresponding to an internal fragment of the 501 bp sequence (hrpZ) gine was used to specific detection of Pseudomonas syringae pv. tomato in tomato seed. A 119 bp PCR product using nested PCR primer was highly specific and sensitive to detect low level of Pseudomonas syrigae pv. tomato in tomato seeds. We believe that the PCR assays developed in this study is very useful to detect Pseudomonas syringae pv. tomato from the tomato seeds.

Phytotoxins of Pseudomonas syringae and PCR Primers for Detection of Phytotoxin-Producing Strains (Pseudomonas syringae의 식물독소와 독소 생산 균주의 검출을 위한 PCR Primer)

  • 정재성;한효심;고영진
    • Research in Plant Disease
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    • v.7 no.3
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    • pp.123-133
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    • 2001
  • Many pathovars of the species Pseudomonas syringae are known to produce different phytotoxins as secondary metabolites. Although phytotoxins generally enhance the virulence of P. syringae, they are not required for pathogenesis. Among the phytotoxins produced by P. syringae, lipodepsipeptides, coronatine, phaseolotoxin, and tabtoxin are the most well-known toxins which have been intensively studied for their structure, mode of action, biosynthesis, and regulation. A polymerase chain reaction (PCR) technique that amplifies a segment of the phytotoxin gene cluster using a primer set has been developed in recent years. This method offers the advantages of speed and sensitivity compared to the approaches based on physiological and biochemical methods. PCR detection of genes involved in the production of toxins could be exploited for early diagnosis of plant diseases caused by P. syringae pathovars.

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Changes of Characteristics Related to Photosynthesis in Soybean Leaves Infected with Pseudomonas syringae pv. glycinea (세균성 점무늬병에 감염된 콩의 광합성 관련 특성 변화)

  • Ryu, Kyung-Yul;Heu, Hoon
    • Korean Journal Plant Pathology
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    • v.11 no.1
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    • pp.61-65
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    • 1995
  • Photosynthetic characteristics of soybean leaves infected with Pseudomonas syringae pv. glycinea were investigated for 8 days. The difference in photosynthesis rate between healthy and diseased soybean leaves decreased for 2 to 4 days after inoculation and then increased. In respiration rate, healthy and diseased leaves showed the same tendency as photosynthetic rate. The stomatal resistance increased following Pseudomonas syringae pv. glycinea infection. The total chlorophyll content of the infected leaf was less than that of the uninfected. Pseudomonas syringae pv. glycinea infection induced the malformation of stacked grana in chloroplast. Dry matter production declined after infection.

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Detection of Pseudomonas syringae pv. actinidiae in Soil on the Basis of PCR Amplification (PCR을 통한 토양에서 Pseudomonas syringae pv. actinidiae의 검출)

  • Han, Hyo-Shim;Koh, Young-Jin;Jung, Jae-Sung
    • Research in Plant Disease
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    • v.10 no.4
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    • pp.310-312
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    • 2004
  • Pseudomonas syringae pv. actinidiae is the causative agent of bacterial canker in kiwifruit. A nested PCR detection method that uses primers designed from the cfl gene, involved in production of the phytotoxin coronatine, was applied on soil samples. These primers yielded 665 and 310-bp fragments in consecutive PCR amplification step with DNA from soil inoculated with Korean strain of P. syringae pv. actinidiae. This system was applied to survey soil samples from a kiwifruit orchard destroyed by bacterial canker. A specific 310-bp PCR product was obtained from all six samples of soil tested.

Occurrence of the strA-strB Streptomycin Resistance Genes in Pseudomonas Species Isolated from Kiwifruit Plants

  • Han Hyo Shim;Koh Young Jin;Hur Jae-Seoun;Jung Jae Sung
    • Journal of Microbiology
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    • v.42 no.4
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    • pp.365-368
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    • 2004
  • The occurrence of strA-strB streptomycin-resistance genes within transposon Tn5393 was examined in Pseudomonas syringae pv. actinidiae, P. syringae pv. syringae, and P. marginalis, isolated from kiwifruit plants in Korea and Japan. PCR amplification with primers specific to strA-strB revealed that three of the tested Pseudomonas species harbored these genes for a streptomycin-resistance determinant. Tn5393, containing strA-strB, was also identified with PCR primers designed to amplify parts of tnpA, res, and tnpR. No IS elements were detected within tnpR, nor were they found in the intergenic region between tnpR and strA. Nucleotide sequence analysis indicated that the strA sequence of P. syringae pv. actinidiae contained a single nucleotide alteration at position 593 (CAA $\rightarrow$CGA), as compared to Tn5393a in P. syringae pv. syringae. This resulted in an amino acid change, from Gin to Arg.

Identification and Characterization of Coronatine-Producing Pseudomonas syringae pv. actinidiae

  • Han, Hyo-Shim;Koh, Young-Jin;Hur, Jae-Seoun;Jung, Jae-Sung
    • Journal of Microbiology and Biotechnology
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    • v.13 no.1
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    • pp.110-118
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    • 2003
  • Pseudomonas syringae pv. actinidiae strains, which cause canker disease in kiwifruit, were collected from kiwifruit orchards in Korea and identified using biochemical and physiological tests. The nucleotide sequences of the 16s rDNA and 16s-23s internally transcribed spacer of the isolates were found to be Identical to those of' the pathotype strain, Kwl 1, of P syringae pv. actinidiae. Remarkably, no coding sequence for phaseolotoxin biosynthesis or phaseolotoxin- resistant ornithine carbamoyltransferase was found by PCR amplification in any of the new Korean isolates of pseudomonas syringae pv. actinidiae, although this was clearly identified in the control pathotype Kwl 1 reference strain. In contrast, three primer sets derived from the coronatine biosynthetic gene cluster and DNA from the Korean strains yielded amplified DNA fragments of the expected size. A sequence analysis of the PCR products revealed that P. syringae pv. actinidiae and the Korean strains of pv. actinidiae contain coronafncate ligase genes (cfl)with identical sequences, whereas their. corR genes exhibited 91% sequence similarity. The production of coronatine, instead of phaseolotoxin, by the Korean strains of P. syringae pv. actinidiae was confirmed by a bioassay using reference pathovars known to produce coronatine and phaseolotoxin. The genes for coronatine biosynthesis in the Korean strains of P. syringae pv. actinidiae were found to be present on plasmids.

Draft genome sequences of Pseudomonas syringae pv. syringae strain WSPS007 causing bacterial shoot blight on apple (사과가지마름병원세균 Pseudomonas syringae pv. syringae WSPS007 균주의 유전체 해독)

  • Lim, Yeon-Jeong;Ryu, Duck Kyu;Kang, Min Kyu;Jeon, Yongho;Park, Duck Hwan
    • Korean Journal of Microbiology
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    • v.55 no.1
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    • pp.80-82
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    • 2019
  • Pseudomonas syringae pv. syringae strain WSPS007 was isolated from infected twigs (Malus pumila) in 2013 in Yeongju, Gyeongbuk Province, Republic of Korea. Here, we report the draft genome sequence of WSPS007 with a chromosome size of 6,238,498 bp (59.04% G+C content). The genome comprises 5,379 CDS, 16 rRNA genes, and 65 tRNA genes. The P. syringae pv. syringae strain WSPS007 genome possesses an ice-nucleating activation (INA) gene and an antifreeze operon that may be related to frost damage by this pathogen. Thus, the genome sequence determined in this study will be useful in understanding the relationship between the outbreak of bacterial shoot blight disease and frost damage in northern Gyeongbuk Province.

Plasmid Profiles of Pseudomonas syringae pv. syringae Isolated from Kiwifruit Plants in Korea and the Copper Resistance Determinant (우리나라에서 분리된 참다래 꽃썩음병 병원세균(Pseudomonas syringae pv. syringae)의 플라스미드와 Cu 저항성 유전자)

  • Park, So-Yeon;Han, Hyo-Shim;Lee, Young-Sun;Koh, Young-Jin;Shin, Jong-Sup;Jung, Jae-Sung
    • Korean Journal of Microbiology
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    • v.43 no.4
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    • pp.337-340
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    • 2007
  • Pseudomonas syringae Pv. syringae is a causal agent of bacterial blossom blight of kiwifruit in Korea. Eleven strains of the pathogen were isolated from different kiwifruit orchards in Korea and the plasmid profiles were obtained by pulsed-field gel electrophoresis. They could be clustered into six groups according to the number and size of plasmids. The number of plasmids per strain and size of these plasmids ranged from 0 to 4 and from 22 to 160 kb, respectively. Among them, four strains belonging to Group III which harbored two plasmids were resistant to copper sulfate. Southern blot hybridization of the plasmid DNA indicated that the copper resistance determinant was carried on a 48 kb plasmid.

Streptomycin Resistant Genes of Pseudomonas syringae pv. syringae, the Causal Agent of Bacterial Blossom Blight of Kiwifruit (참다래 꽃썩음병 병원세균(Pseudomonas syringae pv. syringae)의 스트렙토마이신 저항성 유전자)

  • Park, So-Yeon;Han, Hyo-Shim;Lee, Young-Sun;Koh, Young-Jin;Jung, Jae-Sung
    • Research in Plant Disease
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    • v.13 no.2
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    • pp.88-92
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    • 2007
  • A total of 41 Pseudomonas syringae pv. syringae, the causal agent of bacterial blossom blight, were isolated from kiwifruit plants in Korea. Among them, two strains showing streptomycin resistance were examined to investigate the structure of resistant determinants by PCR and nucleotide sequence analysis. PCR results suggested that the streptomycin resistance is mediated by strA-strB genes carried on Tn5393a. Insertion sequences, IS6100 and IS1133, which were located within or downstream of tnpR gene in Xanthomonas campestris and Erwinia amylovora were not found. Nucleotide sequences of strA-strB were 100% identical with Tn5393a. Two stretomycin resistant strains had three plasmids. Southern blot hybridization using strA-strB probe indicated that the resistant genes were carried on a 100kb plasmid.

Occurrence of Leaf Spot Disease on Watermelon Caused by Pseudomonas syringae pv. syringae (Pseudomonas syringae pv. syringae에 의한 수박 잎점무늬병의 발생)

  • Park, Kyoung-Soo;Lee, Ji-Hye;Kim, Young-Tak;Kim, Hye-Seong;Lee, June-woo;Lee, Hyun-Su;Lee, Hyok-In;Cha, Jae-Soon
    • Research in Plant Disease
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    • v.27 no.4
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    • pp.180-186
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    • 2021
  • Typical bacterial symptoms, water-soaking brown and black leaf spots with yellow halo, were observed on watermelon seedlings in nursery and field of Gyeongnam and Jeonnam provinces. Bacterial isolates from the lesion showed strong pathogenicity on watermelon and zucchini. One of them was rod-shaped with 4 polar flagella by observation of transmission electron microscopy. They belonged to LOPAT group 1. The phylogenical trees with nucleotide sequences of 16S rRNA and multi-locus sequencing typing with the 4 house-keeping genes (gapA, gltA, gyrB, and rpoD) of the isolates showed they were highly homologous to Pseudomonas syringae pv. syringae and grouped together with them, indicating that they were appeared as P. syringae genomospecies group 1. Morphological, physiological, and genetical characteristics of the isolates suggested they are P. syringae pv. syringae. We believe this is the first report that P. syringae pv. syringae caused leaf spot disease on watermelon in the Republic of Korea.