• Korean Journal of Animal Reproduction
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• v.18 no.3
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• pp.217-228
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• 1994

These experiments were carried out to establish the optimal condition of electrostimulatin inducing cell fusion and oocyte activation for nuclear transplantation in mouse embryos. Eggs selected for cell fusion or activation by electrostimulation were equilibrated for 5~10 min. in 0.3M sucrose solution and electrostimulated for 60$\mu$sec using 1 pulse of 60, 70, 80, 90 or 100 volts DC with electrodes 0.2 mm apart. Then they were cultured in 20${mu}ell$ dropsof Tyrode's solution. The results of these experiments are as follows : 1. When one pulse of 60, 70, 80, 90 or 100 volts DC for 60$\mu$sec were applied to 2-cell embryos for fusion of blastomeres, fusion rates were 50.0, 81.7, 91.7, 100 and 100%, respectively ; and developmental rates of fused embryos to blastocyst were 76.7 to 81.5%. Higher fusion rates were observed in 90V and 100V. 2. The average cell number in fused embryos developed to blastocyst was about half of the cell number in diploid controls; and the cell number decreased with increasing of voltages. 3. When pulse numbers were increased, fusion rates improved, but developmental rates were not signficiantly different from the group for which the number of pulse was not increased. And the cell number of blastocyst decreased even more. 4. Oocytes aged for 6hrs after ovulation were electrostimulated for oocyte activation by the same method used for cell fusion. Rates of oocyte activated by electrostimulation were 45.3 to 60.4%, and fragmentation rates were 7.5~15.1%. The lysis rates were 17.0~34.0%. The results of these experiments indicate that the optimal condition for achieving cell fusion and activation is 1 pulse, duration 60$\mu$sec in 90 Volt. The results also show that this condition is suitable for nuclear transplantation using mouse eggs.

• Journal of The Korean Dental Society of Anesthesiology
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• v.12 no.1
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• pp.17-23
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• 2012

Background: This study evaluated pulp vitality of anterior permanent teeth using pulse oximetry (PO), which is already used for monitoring of patient's $SpO_2$ and pulse rates (PR). Also we compared with ice tests and electric pulp test (EPT). Methods: 9 teeth, endodontic treated, were selected as non-vital teeth group. 17 vital teeth were selected as control group. Our aim is to compare sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of ice test, electric pulp test and pulse oximetry, respectively. Pulse oximetry has two test results, $SpO_2$ and pulse rates. Also we calculated correlation and statistical significances by Pearson's test between EPT and pulse oximetry. Results: Sensitivity, specificity, PPV, NPV were calculated on each tests. Ice test has results of 1.00, 0.89, 0.94 and 1.00, respectively. EPT has results of 0.94, 0.78, 0.89 and 0.88 respectively. $SpO_2$ has results of 0.94, 1.00, 1.00 and 0.90, respectively. PR has results of all 1.00. Conclusions: PO showed relatively accurate, stable and objective results on both $SpO_2$ and PR. Percentage of ability of accurate diagnosis for vital teeth is 94% for ice test, 89% for EPT, 100% for $SpO_2$ and PR. Percentage of ability of accurate diagnosis for non-vital teeth is 100% for ice test, 88% for EPT, 90% for $SpO_2$ and 100% for PR. In additions, PR could be more accurate and significant tests than $SpO_2$.

• Journal of Embryo Transfer
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• v.14 no.2
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• pp.107-111
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• 1999

The present study was performed to evaluate the best electric fusion condition in nuclear transfer, Korean Native Cattle fibroblasts were used as nucleic donors. Oocytes from slaughterhouse were matured in vitro for 22 h and enucleated. Each individual cells were transferred into enucleated ocytes and reconstructed embryo were placed into the fusion chamber. In experiment 1, pulse were performed by altering pulse duration at 1. 75kv/cm, 1 time. When pulse duration is 30$mutextrm{s}$, fusion and development rates is higher than other conditions. In experiment 2, the effect of different pulse number were studied at the pulse duration 30$mutextrm{s}$ and the same pulse intensity. When pulse number was one, fusion rates were higher than other conditions. The fused embryos were moved to culture medium and assessed their development to blastocyst. These results showed that best fusion condition was 30$mutextrm{s}$ and one time. And the fibroblasts derived from Han Woo can be reprogrammed by nuclear transplantation and develop subsequently in vitro.

• Proceedings of the KIEE Conference
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• 2002.11d
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• pp.221-223
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• 2002

This paper describes a vacuum tube based high voltage pulse generator for pulsed gas lasers. The pulse generator delivers 20 kV, 300 A pulse outputs with more than 10 kHz pulse repetition rates It can be controlled its Pulse width from 30 ns to 80 ns continuously without any hardware change.

• The Transactions of the Korean Institute of Electrical Engineers C
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• v.52 no.4
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• pp.179-184
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• 2003

The pulsed power system is widely used for many industries and environments. Generally, we call the "RUST", the reddish brown surface, that was made on iron surface or some other metals, when they are contacted by water and air the main substance of rust is oxide-ionization. In other words, the chemical symbol of rust on iron surface is iron oxide(III) hydrate Fe203.nH2O. In this study, we have designed and fabricated our system which has a compact pulse generator with switching MOSFET. Also we have studied the metal-oxide removing characteristics using in the atmospheric arc discharge. It has been investigated their removing characteristics by the change of charging voltage and pulse repetition rates. From this result, we can find out that the removal area Is increased from 3.80 to 8.04[$\textrm{cm}^2$], when pulse duration is increased from 100[pps] to 400[pps]. 400[pps].

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.27 no.6
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• pp.556-559
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• 2001

The aim of the present study was to evaluate the influences of epinephrine and pain on the changes of blood pressure and pulse rates in mandibular 3rd molar extraction. A total of 80 patients(aged between 10 and 30) without systemically compromised conditions were selected and divided into two groups(I, II). 2% lidocaine with 1:100,000 epinephrine was administered in group I (male 20, female 20), and 2% lidocaine without epinephrine was administered in group II (male 20, female 20). Systolic blood pressure and pulse rates were checked by a digital monitor during the pre-local anesthesia state(stage 1), 5 minute later in the post-local anesthesia state(stage 2), the extraction state(during odontectomy, stage 3), and during the post-suture state(stage 4). The measurement of pain perceived by patients was made using a three-point visual analog scale. The results suggest that local anesthetic with epinephrine is better at decreasing pain preventing the severe elevation of systolic blood pressure than local anesthetic without epinephrine. However, these results should be confirmed by additional studies on cardiac patients.

• Journal of Communications and Networks
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• v.7 no.4
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• pp.462-470
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• 2005

We propose a multi-rate and multi-BEP transmission scheme using adaptive overlapping pulse-position modulator (OPPM) and optical power controller in optical code division multiple access (CDMA) networks. The proposed system achieves the multi-rate and multi-BEP transmission by accommodating users with different values of OPPM parameter and transmitted power in the same network. The proposed scheme has advantages that the system is not required to change the code length and number of weight depending on the required bit rate of a user and the difference of bit rates does not have so much effect on the bit error probabilities (BEPs). Moreover, the difference of transmitted powers does not cause the change of bit rate. We analyze the BEPs of the four multimedia service classes corresponding to the com­binations of high/low-rates and low/high-BEPs and show that the proposed scheme can easily achieve distinct differentiation of the service classes with the simple system configuration.

• Proceedings of the Optical Society of Korea Conference
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• 2000.08a
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• pp.18-19
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• 2000

In this paper, packet error rates of an all-optical packet switching node, which uses a fiber-optic delay-line matched filter as the optical packet header processor, has been calculated for the various optical pulse shapes.

• Proceedings of the Acoustical Society of Korea Conference
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• 1998.08a
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• pp.136-141
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• 1998

In order to enhance the performance of a CELP coder at low bit rates, it would be necessary to make the CELP excitation have the peaky pulse characteristic. In this paper we introduce an excitation signal with peaky pulse characteristic. It is obtained by using a two-tap pitch predictor. Samples of the signal have different gains according to their amplitudes by the predictor. In voiced sound the signal has the desirable peaky pulse characteristic, and its periodicity is well reproduced. Particularly, peaky pulses at voiced onset and a burst of plosive sound are clearly reconstructed.

• Proceedings of the KIEE Conference
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• 1998.07g
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• pp.2255-2258
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• 1998

Method of diagnosis in oriental medicine, the unbalance of the physiological function of the five viscers and six bowels of the human body is determined from time immemorial with the condition of blood circulation which is performed through blood vessels by the vitality of the heart. In oriental medicine, treatment is largely attempted by adjusting this unbalance. The analysis of pulse wave, which mainly measures the changes in blood flows, is to evaluate the shapes of a pulse wave rather than the quantitative changes like rates and strength of the pulse. This paper presents the development of Hardware System and Pulse Diagnosis Algorithm for automatic diagnosis of the pulse wave. This system makes the precise diagnosis and the objective recording possible.