• Journal of Korean Society of Forest Science
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• v.99 no.6
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• pp.885-890
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• 2010

This study was conducted to confirm the application as ingredients of cosmetics through an examination of the function for anti-oxidant activity of the fraction isolated from Pyrus ussuriensis leaves. The dried leaf of Pyrus ussuriensis were extracted with acetone-$H_2O$ (6:4, v/v), concentrated and fractionated with the upper layer of acetone on a separatory funnel. Each fraction was freeze dried, then a portion of acetone soluble powder was chromatographed on a Sephadex LH-20 column using a series of aqueous methanol as eluents and also used the MIC-gel using a series of aqueous methanol as developing solvent. The isolated compounds were identified by silica-gel TLC. The concentration of total phenolic compound of Pyrus ussuriensis acetate soluble fraction was high, 914 mg/g. The results obtained from the analyses of the anti-oxidanat effects of Pyrus ussuriensis acetate fraction can be summarized as follows: In the result of DPPH scavenging radical activity, Pyrus ussuriensis acetate soluble fraction showed more than 80% at 100 ppm. SOD-like activity of one of Pyrus ussuriensis acetate soluble fractions was 77% at 1000 ppm. Xanthine oxidase inhibition of Pyrus ussuriensis acetate soluble fraction was 38% at 100 ppm. From these results, we confirmed that acetate fraction of Pyrus ussuriensis has a great potential as a natural ingredients with a natural antioxidant and antimicrobial source.

• Korean Journal of Medicinal Crop Science
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• v.7 no.4
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• pp.303-307
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• 1999

Three antioxidative compounds were isolated from the EtOAc extract of the fruits of Pyrus ussuriensis. On the basis of spectroscopic evidences, the structures of these compounds were established as 1, 4-dibenzenediol, chlorogenic acid and quercitrin. The DPPH free radical scavenging activities of 1, 4-dibenzenediol $(RC_{50}\;:\;0.4{\mu}g)$ and chlorogenic acid $(RC_50\;:\;4{\mu}g)$ were more effective than those of BHA $(RC_{50}\;:\;14{\mu}g)$ and a-tocopherol $(RC_{50}\;:\;12{\mu}g)$

• Korean Journal of Plant Resources
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• v.34 no.4
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• pp.377-383
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• 2021

In this study, we investigated in vitro immunostimulatory activity of fruit extracts from Pyrus ussuriensis var. hakunensis (Nakai) T.B. Lee (PUF) using mouse macrophage RAW264.7 cells. PUF increased the production of immunostimulatory factors such as NO, iNOS, IL-1β, IL-6 and TNF-α, and phagocytic activity in RAW264.7 cells. The inhibition of TLR2 and TLR4 blocked PUF-mediated production of immunostimulatory factors in RAW264.7 cells. In addition, the inhibition of MAPKs signaling pathway reduced PUF-mediated production of immunostimulatory factors. From these results, PUF may have immunostimulatory activity via TLR2/4-mediated activation of MAPKs signaling pathway. Therefore, PUF expected to be used as a potential immune-enhancing agent.

• Journal of Korean Society of Forest Science
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• v.81 no.4
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• pp.357-362
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• 1992

To understand water flow path in the cells of Robinia pseudoacacia and Pyrus ussuriensis and obtain color wood from them, this experiment was performed by penetrating 0.5% acidic fuchsin and 0.5% fast green solution into the living wood of them. A comparison was made of the wood structure and water flow path from Robinia pseudoacacia containing the ring-porous wood with tyloses, and Pyrus ussuriensis including solitary diffuse-porous wood with distinct fiber pits. The dye penetrated into vessel elements in the early wood of two growth rings from bark in Robinia pseudoacacia, but permeated all xylem in fonts ussuriensis. In Robinia pseudoacacia, the vessels of heart wood and intermediate wood were not stained because of tyloses.

• Journal of Korean Society of Forest Science
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• v.99 no.4
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• pp.546-552
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• 2010

The aim of the study was to assess the cosmeceutical activity of Pyrus ussuriensis leaves and it is possible that can be used as a cosmetic ingredient for application of cosmetic industries. P. ussurensis leaf was extracted with various solvents including water, 70% ethanol and 60% acetone. In the result of DPPH (1,1-diphenyl-2-picryl-hydrazyl) scavenging radical activity, water, ethanol and acetone extract of P. ussuriensis leaf were higher than 70%, 80% and 85% at 50 ppm concentration, respectively. Xanthine oxidase inhibition activity and superoxide dismutase (SOD)-like activity by P. ussuriensis extract were higher than 30%. In addition, SODlike activity of all extracts showed tendency of the significant increase with the increase of concentration. In the anti-inflammatory test, P. ussuriensis leaf extract inhibited generation of nitric oxide (NO) stimulated by LPS in the macrophage cell line (raw 264.7) after 12 to 24 hours. As above results, P. ussuriensis has a great potential as a cosmeceutical raw material as well as anti-oxidant and anti-inflammatory ability.

• Journal of Korean Society of Forest Science
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• v.100 no.2
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• pp.136-141
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• 2011

This study was conducted to confirm the application as ingredients of cosmetics through an examination of the function for anti-cancer and anti-microbial of the fraction isolated from Pyrus ussuriensis leaves. The dried leaf of P. ussuriensis were extracted with acetone-$H_{2}O$ (6:4, v/v), concentrated and fractionated with the upper layer of acetone on a separatory funnel. Each fraction was freeze dried, then a portion of acetone soluble powder was chromatographed on a Sephadex LH-20 column using a series of aqueous methanol as eluents and also used the MIC-gel using a series of aqueous methanol as developing solvent. The isolated compounds were identified by silica-gel TLC. The growth inhibition activity was measured using the MTT assay by the mouse meltioma (B16F10) cell. The cancer cell growth inhibition rate of fractions isolated from P. ussuriensis leaf was 80%. In anti-microbial activity test, the fraction of P. ussuriensis with 0.25 mg/disc resulted in the clear zone of 1.3 cm and 2 cm for Staphylococcus aureus and S. epidermidis of gram positive bacillus, respectively. In Escherichia coli of gram negative bacillus, the fraction with 0.5 mg/disc resulted in the clear zone of 1.1 cm~1.5 cm each fraction. From these results, we confirmed that acetate fraction of P. ussuriensis has a great potential as a natural ingredients with a anti-cancer and anti-microbial source.

• Journal of Forest and Environmental Science
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• v.37 no.3
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• pp.226-234
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• 2021

Two species, P. pyrifolia and P. ussuriensis, of the genus Pyrus native to Korea, are valuable genetic resources that can be used for food, dietary supplement, pharmaceutical, and cosmetics. Bioactive compounds of the plant leaves are the main components that are used for the products. Farmers had cultivated a few individuals from the wild to produce fruits or leaves for traditional remedy or tea; however, bioactive components of their leaves are not tested. We selected some trees from the natural stand that have distinct traits for the improvement program. We investigated the bioassay on the extracts' bioactive compounds and antioxidant capacity from the selected accessions and other accessions, including newly developed cultivars. The contents of the phenolic compounds and flavonoids from the leaf extracts of the selected accessions were higher than the commonly cultivated trees in both species but lower than 'Sanhyang' in P. ussuriensis. The antioxidant capacity was measured using two assay methods, DPPH and ABST. The selected cultivars also had higher inhibitory activity than common trees. The selected accession 'Cultivar 3' in P. pyrifolia had the highest radical scavenging activity than others. Although leaves of the accessions were used only in this study, all three selected individuals have the potential for cultivar in containing high bioactive compounds and antioxidant capacity.

• Plant Resources
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• v.4 no.3
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• pp.130-139
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• 2001

Four major naturally populated areas around the Jindong valley in Inje-Gun, Gangwon province for wild Pyrus ussuriensis, Malus sieboldii, and Malus baccata are mostly located on the southeast side of the mountains at 630-745m in altitude at sea level and are in very good sunny areas. The total of 77 taxa dividing into 32 families, 58 genera, 65 species, and 12 varieties has been inhabited in these areas. Most of these plants are heliophytes, which love sunshine, but some of rhizo-plants such as Sasa borealis, climbing plants such as Actinidia arguta and Celastrus orbiculatus, or naturalized plants like Bidens frondosa are also shown. These areas have been continually destroyed, so it is possibly thought that the second transition has been progressed. The index of species diversity of plant groups in these areas is 0.672∼1.465 based on the Shannon-Wiener's method, but its index for the area that rhizo-plants like Sasa borealis are growing as an under planting decreases relatively. In this study, the oldest plant of Pyrus ussuriensis in Korea, which is 120cm in diameter at breast height (DBH), 19m in tree height and 25m in width, has been found.

• Korean Journal of Environment and Ecology
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• v.16 no.2
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• pp.160-171
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• 2002

In order to clarify the species composition, distribution and habitat of the Korean wild Pyrus ussuriensis and P. pyrifolia communities, that are so expected as a useful resource plant, an investigation was carried out according to phytosociological method on Mts. Hambaek, Ilwol, Sobaek, Juwang and Gaya in Korea. In the present study, we made an attempt to elucidate the autecological characteristics of the wild Pyrus species by synecological approach. As a result, the forests including the Pyrus species were divided into the two associations and two communities, considering the existing vegetation units for the forest; Syneilesio-Quercetum serratae, Corylo-Quercetum mongozicae, Ainsziaea acerifolia- Quercus mongolica community and Fraxinus rhynchophylla-Pyrus ussuriensis community. Among the units, the Syneilesio-Quercetum serratae and the Corylo-Quercetum mongolicae belong to southern type and middile-northern type, respectively, in their distributional type. It is inferred that the wild Pyrus species are distributed preferably in the deciduous forest zone in Korea, compared to the other forest zones. In particular the Pyrus species are present alone or dominantly in only both the tree layer or subtree layer of the forests, reflecting their shade intolerance ecophysiologically. Thus they usually were distributed in SE or SW of slope aspect. Generally the canopy of the forests including the Pyrus species was so open. Species diversity of the vegetation units was highest in the Syneizesio-Quercetum serratae and lowest in the Ainsliaea acerifolia-Quercus mongolica community. The Corylo-Quercetum mongolicae and the Pyrus ussuriensis-Fraxinus rhynchophylla community are medium between the two.

• Plant Resources
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• v.4 no.3
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• pp.157-160
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• 2001

The wild Pyrus plants belong to the deciduous woody plants in Rosaceae family. Most are cultivated for fruit productions, but some for ornamental plants. There are 5 Pyrus species including Pyrus ussuriensis and 8 varieties cultivated in Korea. In Korea, these plants are populated near villages or on the hills, and they are easily propagated since their fruit are very juicy and quite big in size. Plant breeding to get improved cultivars is not difficult because the Pyrus plants are easy to cross breeding among its genus. Because not only this reason but there have been shown lots of variation in wild groups, the Pyrus plants inhabited in Korea are excellent deciduous woody plants and favorable fruit trees to develop ornamental plants. It is necessary to develop many kinds of fruit trees and ornamental trees containing genetically different character, so that it will be satisfied for many different people's taste and demands. Collecting lots of wild plant resource with diverse genes is necessary for plant breeding.