• Title/Summary/Keyword: R-determinant replication origin

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Characterization of R plasmid pKU 41 from pseudomonas putida KU190 (Pseudomonas putida KU 190에서 분리한 R plasmid pKU 41의 특성)

  • 이윤희;주미자;이영록
    • Korean Journal of Microbiology
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    • v.26 no.1
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    • pp.13-19
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    • 1988

  • The location of R-determinants, $Ap^{r}$ and $Tc^{r}$, and replication origin in pKU41 determined using the construction of miniplasmid by the BamHI and the HindIII restriction fragment from pKU41 and the cloning of the restriction fragments from pKU41 into pSY343. The gene encoding resistance to ampicillin (Ap) as well as replication origin in pKU41 were located on the region overlapping BamHI B fragment and HindIII A fragment. The gene encoding resistance to tetracycline (Tc) was located on the region of the HindIII C fragment, which was cleaved by BamHI as well.

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