• Journal of Animal Science and Technology
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• v.45 no.4
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• pp.537-542
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• 2003

Sixty [(Duroc${\times}$Yorkshire)${\times}$Landrace] pigs (7.63$\pm$0.41kg average body weight and 25-d average age) were used in a 20-d growth assay to determine the effect of dietary recombinant human lactoferricin culture (RHLC) supplementation on growth performance, digestibility and plasma IgG concentration in weaning pigs. Dietary treatments included 1) Negative control (NC : without antibiotic), 2) Positive control (PC : NC diet + 0.1% chlortetracycline), 3) RHLC0.3 (NC diet + 0.3% RHLC), 4) RHLC0.5 (NC diet + 0.5% RHLC). No differences were found among treatments in average daily gain (P>0.05). ADFI of pigs fed RHLC0.3 diet was higher than that of pigs fed PC diet (P<0.05). However, pigs fed RHLC0.5 diet had improved gain/feed compared to pigs fed PC diet. Pigs fed PC and RHLC diets showed significantly increased dry matter digestibility compared to pigs fed NC diet (P<0.05). There was no significant difference in plasma IgG concentrations (P>0.05). The supplementation of RHLC in starter pig diets appears to be an alternative to antibiotics.

• Korean Journal of Agricultural Science
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• v.28 no.2
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• pp.92-98
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• 2001

Lactofenicin is an antibacterial peptide fragment (about 5 kD) derived from lactoferrin (80 kD) that displays the various biological functions. The production of a human lactoferricin (Lactoferricin H) in mouse HC11 mammary epithelial cells was achieved by placing its cDNA under the control of the bovine ${\beta}$-casein gene. To express lactoferricin H in this cell culture system, constructed a hybride-splice signal consisting of bovine ${\beta}$-casein intron I and rabbit ${\beta}$-globin intron II, and a DNA fragment spanning intron 8 of the bovine ${\beta}$-casein gene. Expression of lactofenicin H from this expression vector was identified by RT-PCR, northern and dot blot analysis. RT-PCR using total RNA of HC11 cells transfected with pBL1-cin expression vector yielded a product identified as having a size of the 150bp. Northern blot analysis was identified about 2.3 kb. In dot blot analysis, recombinant lactofenicin H was recognized with anti-human lactofrrnin polyclonal antibody.