• Proceedings of the Korean Society of Fisheries Technology Conference
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• 1998.06a
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• pp.355-356
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• 1998

• Journal of Korean Society on Water Environment
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• v.34 no.3
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• pp.285-292
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• 2018

This study was performed in order to identify the red tide-causing organism and to understand the characteristics of the water quality during the winter of 2015 and 2016 in the Oncheon stream, a tidal river in Busan, where red tide often occurs in the wintertime. Two sites were selected on the stream and the surface water was sampled a total of 28 times during the experimental period. Twelve water quality characteristics, including water temperature, pH, DO, COD, total-N (T-N), total-P (T-P), and salinity were analyzed in order to test water quality. The cell numbers of cryptomonads were counted directly by microscopic observation. The nucleotide sequences of the partial 28S rRNA gene and psbA gene from metagenomic DNA, derived from each sampling site, were analyzed. According to the results, the alga most responsible for the bloom was identified as Teleaulax OC1 sp., which belongs to the cryptomonads. Three items of chl-a, pH, and DO were positively correlated with the cell numbers of the cryptomonads counted at the upper stream of the tidal area (St 1) while eight items of chl-a, TOC, BOD, total-N, COD, SS, pH, and DO were positively correlated with the cells located at the junction between the stream and Su-young river (St 2) in the order.

• ALGAE
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• v.18 no.4
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• pp.361-363
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• 2003

This study was conducted at Seomjin River estuary to identify the causative species of algal bloom and their blooming cycles. Field surveys were conducted at 4 stations in every week from April to December of 1999. Thirty species were observed as the causative species of alga bloom. Skeletonema costatum, Thalassiosira sp., and microflagellate spp. (mixed red tide: Chroomonas sp. and two species of Prasinophycea) made algal blooms during the present study period. In addition, toxic algal species of diatom Pseudo-nitzschia multiseries and dinoflagellate Dinophysis acuminata were observed. The algal blooms were caused by microflagellate spp. in June, Thalassiosira sp. in July and Skeletonema costatum in August. Generally, the algal blooms persisted for about 5 days in this area.

• Fisheries and Aquatic Sciences
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• v.1 no.2
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• pp.250-254
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• 1998

Harmful micro algae isolated from Korean coastal waters, were tested with FITC-conjugated lectins and observed by epifluorescent microscopy to distinguish each other. Strain-specific sugar composition at the cell surface was suggested by the affinity of lectins to different microalgae. The micro algae Cochlodinium polykrikoides (CP-1) and Gymnodinium $A_3\;(GA_{3-1}\;1)$, are morphologically similar, but exhibited different binding activity with the lectins ECA, HPA and WGA. In Peridiniales, the micro alga Alexandrium tamarense (AT) bound HPA and WGA, but Scrippsiella trochoidea (ST-1) did not bind those lectins. Three species of Prorocentrum also exhibited different binding specificity with HPA, PHA and SBA. A non­toxic Korean isolate of Heterosigma akashiwo (HA-2) bound ConA, PEA and UEA. These results suggest that lectins are useful in discriminating morphologically similar species, as well as different species or strains within the same genus.

• ALGAE
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• v.29 no.2
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• pp.101-109
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• 2014

Small red algae, especially those previously referred to as 'primitive' are often overlooked, but can be quite abundant. These 'primitive' red algae are now placed in several classes distinct from the Florideophyceae, for example the Stylonematophyceae. A brownish-red filamentous alga was collected from a sandy tide pool at Cape Tribulation, Queensland, Australia. Cultured specimens were identified as Bangiopsis and conformed to the morphological characters of the genus (multicellular base, erect filaments branched or unbranched, uniseriate to multiseriate-tubular, single multilobed purple-red to red-brown plastid with central pyrenoid, vegetative cells released directly as spores). Molecular data of two plastid genes (rbcL, psbA) support placement of the Australian isolate and isolates from India in Bangiopsis. The genetic variation between these isolates and isolates from Puerto Rico previously attributed to B. subsimplex indicates that these should be considered as a separate species. As the type locality is in the Atlantic Ocean, French Guiana, and not far from Puerto Rico, and the Puerto Rican isolate has been used often in phylogenetic analyses, we propose that the Indian and Pacific Ocean isolates be designated a new species, B. franklynottii, to acknowledge Ott's many years of research on inconspicuous freshwater and marine red algae. Our research also highlights the lack of careful descriptions in many of the records of this genus and the lack of morphological characters to distinguish species. Especially within the morphologically simple red algae, morphological distinctness does not necessarily reflect evolutionary divergences.

• Journal of Korean Society of Environmental Engineers
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• v.32 no.1
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• pp.53-60
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• 2010

In the present study, experiments have been performed to investigate the possibility of removing Cochlodinium polykrikoides using the dredged sediment from a coastal fishery and then to derive the optimal conditions; the amount and particle size of dredged sediment besprinkled into water, the thermal treatment, the types and amounts of additives, and the depth profile of Cochlodinium polykrikoides. Results showed that the optimal amount of dredged sediment besprinkled into water was 6~10 g/L, and the removal efficiency of Cochlodinium polykrikoides after the reaction time for 60 min was 73~93%. Note that, in the real sea water, it is necessary to besprinkle 6~10 $kg/m^3$ of dry dredged sediment on a unit area (1 $m^2$). With decreasing particle size, Cochlodinium polykrikoides could be more efficiently removed. The removal efficiency was 93% with the dredged sediment smaller than 100 ${\mu}m$, whereas it was 51% with that of 100 ${\mu}m$ ${\mu}m$. Since most of dredged sediment (over 90%) was smaller than 100 ${\mu}m$, high efficiency could be obtained by besprinkling only the dredged sediment without pre-treatment. CaO was found to be an effective additive in promoting the removal efficiency (up to 99%). The optimal amount of additive was 5~10%, however, it was necessary to use as small amount of an additive as possible in order to avoid the sharp increase in pH. The removal efficiency increased with increasing depth profile of Cochlodinium polykrikoides. The removal efficiency was 83% at 5 cm depth, whereas it was 93% at 50 cm depth. In the sea water, red tide occurred within 3 m depth, and furthermore most Cochlodinium polykrikoides existed within 1 m depth. It was, therefore, expected that higher removal efficiency of Cochlodinium polykrikoides could be obtained when the dredged sediment was besprinkled into the sea water. The removal efficiency of Cochlodinium polykrikoides was up to 93% when the dredged sediment (<100 ${\mu}m$) was besprinkled into water at the ratio of 10 g/L. This result was comparable to that obtained with loess (90~97%). All the results in the present study indicated that the dredged sediment from a coastal fishery could be successfully used as a substitute of loess for removing the red tide alga.

• Journal of Chitin and Chitosan
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• v.22 no.3
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• pp.185-193
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• 2017

Red tide Heterosigma akashiwo (H. akashiwo), a microscopic alga of the class Raphidophyceae, causes extensive damage to all marine ecosystems. It is essential to reduce the damage to marine ecosystems for them to be used as a resource. In this study, we used organic solvent fractionation to obtain an ethyl acetate-methanol extract from H. akashiwo (HAEM80) and then evaluated its anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and a zebrafish model. HAME80 markedly inhibited the production of nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$). It also down-regulated the protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) and decreased the secretion of interleukin-$1{\beta}$ ($IL-1{\beta}$) in LPS-stimulated RAW 264.7 cells. HAME80 reduced yolk edema and improved the survival rate of LPS-stimulated zebrafish embryos; in addition, the extract significantly reduced the production of ROS and NO and attenuated cell death in this model. Gas chromatography-mass spectrometry (GC-MS) of the extract was used to confirm the identity of peaks 1-20. Taken together, our data suggest that H. akashiwo is a beneficial anti-inflammatory agent.

• Journal of the Korean Society for Marine Environment & Energy
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• v.19 no.4
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• pp.341-348
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• 2016

Micro-algae, one of the biological resources for alternative energy, has been heavily studied. Among various methods to analyze the status of the micro-algae including counting, screening, and flocculation, the flocculation approach has been widely accepted in many critical applications such as red tide removal study or microalgae resource study. To characterize the flocculation status of the micro-alga. A traditional optical modality, i.e., photospectrometry, measuring the optical density of the flocs has been frequently employed. While this traditional optical method needs shorter time than the counting method in flocculation status analysis, it has relatively lower detection accuracy. To address this issue, a novel real-time micro-algae flocculation analysis method based on the lens-free shadow imaging technique (LSIT) is introduced. Both single cell detection and floc detection are simultaneously available with a proposed lens-free shadow image, confirmed by comparing the results with optical microscope images. And three shadow parameters, e.g., number of flocs, effective area of flocs, and maximum size of floc, enabling quantification of the flocculation phenomenon of micro-alga, are firstly demonstrated in this article. The efficacy of each shadow parameter is verified with the real-time flocculation monitoring experiments using custom developed cohesive agents.

• Korean Journal of Environmental Biology
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• v.36 no.3
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• pp.359-369
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• 2018

Harmful algal blooms (HABs) are a serious problem for public health and fisheries industries, thus there exists a need to investigate the possible ways for effective control of HABs. In the present study, we investigated the algicidal effects of a newly developed GreenTD against the HABs (Chattonella marina, Heterosigma akashiwo, Cochlodinium polykriokides, and Heterocapsa circularisquama) and non-HABs (Chaetoceros simplex, Skeletonema sp. and Tetraselmis sp.), which is focused on the different population density and concentration gradients of algicidal substances. The time series viability of target alga was assessed based on the activity of Chl. a photosynthetic efficiency in terms of $F_v/F_m$, and in vivo fluorescence (FSU). Effective control of Raphidophyta, C. marina and H. akashiwo was achieved at a GreenTD concentration of $0.5{\mu}gL^{-1}$ and $0.2{\mu}gL^{-1}$, respectively, and regrowth of both the species was not observed even after 14 days. The inhibitory ratio of the dinoflagellate, C. polykriokides was more than 80% at $0.2{\mu}gL^{-1}$ of GreenTD. H. circularisquama was constantly affected in the presence of $0.2{\mu}gL^{-1}$ of GreenTD in the high- and low-population density experimental groups. On the other hand, diatoms, C. simplex, and Skeletonema sp. were not significantly affected even in the presence of $0.2{\mu}gL^{-1}$ of GreenTD and exhibited re-growth activity with the passage of incubation time. In particular, green alga Tetraselmis sp. remained unaffected even in the presence of the highest concentration of GreenTD ($1.0{\mu}gL^{-1}$), implying that non-HABs were not greatly influenced by the algicidal substances. As a result, the algicidal activity of GreenTD on the harmful and nonharmful algae was as follows: raphidophyte>dinoflagellates>diatoms>green alga. Consequently, our results indicate that inoculation of GreenTD substances into natural blooms at a threshold concentration ($0.2{\mu}gL^{-1}$) can maximize the algicidal activity against HABs species. If we consider the dilution and diffusion rate in the field application, it is hypothesized that GreenTD will demonstrate economic efficiency, thus leading to effective control against the target HABs in the closed bay.