• Animal cells and systems
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• 제16권3호
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• pp.183-189
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• 2012

It has been suggested that chromatin is organized into the stable structures that provide fundamental units of chromosome architecture in interphase mammalian cells. The stable structures of chromatin can be visualized as replication foci when replicating DNA is labeled with thymidine analogs. Previously, we showed that the chromosome territory expanded after BAF53 knockdown. In this study, we found that BAF53 is required for the formation of replication foci. DNA replication was not impaired in BAF53 knockdown cells, suggesting that the decrease in the number of replication foci is due to disintegration of replication foci, but not suppression of DNA replication. The attractive forces that maintain structural integrity of replication foci could be disrupted by BAF53 knockdown, and it may be responsible, at least in part, for the expansion of chromosome territories after BAF53 knockdown.

• BMB Reports
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• 제40권6호
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• pp.895-898
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• 2007

The oxygen dependent regulation of DNA replication is an essential property of proliferating mammalian cells. In human T24 bladder cancer cells, several hours of hypoxia leads to reversible DNA replication arrest and re-entry of oxygen induces a burst of replication initiation. This short communication provides strong evidence that PD184352 initiates DNA replication in living hypoxic cells without elevating the oxygen level. PD184352 releases the regular hypoxic replicon arrest, however, at a low intensity compared to the effect of reoxygenation. Moreover, PD184352 shows no effect on normoxically incubated as well as reoxygenated T24 cells.

• 생명과학회지
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• 제11권4호
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• pp.371-378
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• 2001

The effects of the RM 60 series on DNA replication systems were examined by using simian virus 40 (SV40) DNA replication system in vitro. The RM 60 series inhibited the DNA replication in the initiation step rather than in the elongation step. Polymerase$\alpha$-primase activity and toposiomerase I activity study were performed subsequently, the RM 60 seies increased the polymerase $\alpha$-primase activity in a low dose, but decreased the activity in a higher dose. The topoisomerase I activity was inhibited by the RM 60 series. This result suggests that the RM 60 series might inhibit some molecules which are required to establish replication forks during the initiation step of the DNA replication.

• Journal of Electrical Engineering and Technology
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• 제13권6호
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• pp.2447-2455
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• 2018

Security is more important in many sensor applications. The node replication attack is a major issue on sensor networks. The replicated node can capture all node details. Node Replication attacks use its secret cryptographic key to successfully produce the networks with clone nodes and also it creates duplicate nodes to build up various attacks. The replication attacks will affect in routing, more energy consumption, packet loss, misbehavior detection, etc. In this paper, a Secure-Efficient Centralized approach is proposed for detecting a Node Replication Attacks in Wireless Sensor Networks for Static Networks. The proposed system easily detects the replication attacks in an effective manner. In this approach Secure Cluster Election is used to prevent from node replication attack and Secure Efficient Centralized Approach is used to detect if any replicated node present in the network. When comparing with the existing approach the detection ratio, energy consumption performs better.

• 한국동물학회지
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• 제37권4호
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• pp.437-477
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• 1994

The DNA replication of human Iyrnphocvtes was studied using Bromodeo3fyuridine incorporation. The characteristic patterns of dvnamlc banding were analysed. Human chromosomal ONA was synthesized in a segmental but highly coordinated fashion. Each chromosome replicates according to its innate pattern of chromosome structure (bandinsl. R-positive bands are demonstrated as the initiation sites of DNA synthesis, and G-bnads initiate replication after it has been completed in the autosomal R-bands. Many researchers demonstrated that developmental or induced methvlation of DNA can inactivate the associated gene loci. Such DNA methylation can be reversed and specific genes reactivated by treatment with 5-azacvtidine. We treated the hvpomethvlating agent 5-azacvtidine and tested for changes of DNA replication pattern. Treatment with 5-azacytidine causes an advance in the time of replication. These observed changes in timing of replication suggest that DNA methvlation may modify regional groups of genes in concert.

• 미생물과산업
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• 제11권1호
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• pp.13-20
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• 1985

특이 단백질(gene II protein)의 정성적 또는 정량적 변화에 의해 DNA 복제에 필요한 replication orgin sequence의 일부분이었단 replication enhancer를 불필요하게 함으로써 minimum replication origin을 core region만으로 국한 되게 함이었다.

• Journal of Microbiology and Biotechnology
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• 제17권11호
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• pp.1841-1847
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• 2007

We previously identified the origin of replication of p703/5, a small cryptic plasmid from the KBL703 strain of Enterococcus faecalis. The origin of replication contains putative regulatory cis-elements required for replication and a replication initiator (RepA) gene. The replicon of p703/5 is similar in its structural organization to theta-type plasmids, and RepA is homologous to a family of Rep proteins identified in several plasmids from Gram-positive bacteria. Here, we report molecular interactions between RepA and the replication origin of p703/5. DNase I footprinting using recombinant RepA together with electrophoretic mobility shift assays confirmed the binding of RepA to the replication origin of p703/5 via iterons and an inverted repeat. We also demonstrated the formation of RepA dimers and the different binding of RepA to the iteron and the inverted repeat using gel filtration chromatographic analysis, a chemical crosslinking assay, and electrophoretic mobility shift assays in the presence of guanidine hydrochloride. Our results suggest that RepA plays a regulatory role in the replication of the enterococcal plasmid p703/5 via mechanisms similar to those of typical iteroncarrying theta-type plasmids.

• BMB Reports
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• 제44권6호
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• pp.393-398
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• 2011

Polydnaviruses are a group of double-stranded DNA viruses and are symbiotically associated with some ichneumonoid wasps. As proviruses, the replication of polydnaviruses occurs in the female reproductive organ at the pupal stage. This study analyzed the effects of two developmental hormones, juvenile hormone (JH) and ecdysteroid, on the viral replication of Cotesia plutellae bracovirus (CpBV). All 23 CpBV segments identified contained a conserved excision/rejoining site ('AGCTTT') from their proviral segments. Using quantitative real-time PCR based on this excision/rejoining site marker, initiation of CpBV replication was determined to have occurred on day 4 on the pupal stage. Pyriproxyfen, a JH agonist, significantly inhibited adult emergence of C. plutellae, whereas RH5992, an ecdysteroid agonist, had no inhibitory effect. Although RH5992 had no effect dose on adult development, it significantly accelerated viral replication. The results of immunoblotting assays against viral coat proteins support the effects of the hormone agonists on viral replication.

• 미생물과산업
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• 제12권1호
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• pp.28-34
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• 1986

이글은 Esser, K. & Oeser, B.의 논문으로 해외근착자료로 소개한다. Nucleic acids do not only carry the genetic information, but are also the only substances being able of self-replication. Molecular cloning, an essential tool in biotechnology, requires among other things, an understanding of the mechanisms of replication which at present is fairly well known. After an introduction to the general principle, the status of art on replication procedure and its implication for biotechnology are dealt with.

• 한국정보과학회논문지:정보통신
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• 제30권4호
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• pp.545-558
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• 2003

이동단말의 사용이 보편화 됨에 따라 통신 서비스의 개인화 및 맞춤화 요구가 증가하고 있다. 개인화 서비스의 궁극적인 목표는 사용자가 임의의 망으로 로밍해 가더라도 당초 가입한 서비스와 유사한 형태로(same look and feel) 서비스를 제공하는 가상 홈 환경의 실현에 있다. 가상 홈 환경에서는 개인의 서비스를 정의하는 서비스 프로파일의 관리가 필수 절차가 된다. 이러한 가상 흠 환경을 구현하기 위한 두 가지 방법은 가입자가 로밍하는 곳에 프로과일을 중복하여 방문망 내에서 서비스론 제공하는 무조건추종중복[Follow-Me Replication Unconditional(FMRU)] 기법과, 홈 망의 서비스시스템으로부터 서비스를 실행하는 중앙집중(Central) 기법으로, 전자는 call-to-mobiligy ratio(CMR)이 높은 가입자에게, 후자는 CMR이 낮은 가입자에게 적합하다. 그러나 해당 가입자가 어떤 경우에나 고정된 CMR을 갖는 것은 아니므로 평균적인 CMR로 위 두 기법을 선택하여 적용하는 것은 성능 저하의 문제가 있다. 본 논문에서는 가상 홈 환경에서 서비스 프로파일 관리를 위한 두 가지 기법을 구체화하고, FMRU를 개선한 요구기반 무조건추종중복[Follow-Me Replication Unconditional on-Demand(FMRUD)]기법을 제시하였다. 또한 가입자의 변화하는 CMR에 따라 자동적으로 두 기법을 선택하도록 하는 적응적 추종 중복[Adaptive Follow-Me Replication(AFMR)]기법을 제안하였으며 이들을 비교하였다. 반응 수식을 통한 성능분석 결과 프로파일 크기 관점에서 효율성을 판단하며 CMR 변화를 고려하는 AFMR이 더욱 높은성능을 가짐을 알 수 있다.