• The Korean Journal of Mycology
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• v.41 no.3
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• pp.181-184
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• 2013

Rhodosporidium fluviale 201-C-1 and Kluyveromyces thermotolerans 150-JE-2(1) were isolated from wild flowers in Geumsan-gun, Chungcheongnam-do and Wando-gun, Jeollanam-do, respectively. Rhodosporidium paludigenum 86-J-1 and Candida sp. 80-J-3 were also isolated from flowers in Jeju island, Korea. Characteristics for these yeasts were not reported before. Therefore, morphological and physiological characteristics for these yeasts were investigated in this study. R. fluviale 201-C-1 was halophilic asporogenous yeast and formed pseudomycelium. It also grew at $30^{\circ}C$ and pH 4.0-6.0. K. thermotolerans 150-JE-2(1) was sugar-tolerant and halotolerant asporogenous yeast. It formed pseudomycelium and showed positive urease activity. Candida sp. 80-J-3 and R. paludigenum 86-J-1 were asporogenous yeasts, and grew well in yeast extract-peptone dextrose(YPD) medium, potato-dextrose medium as well as vitamin-free medium and YPD medium containing 5% NaCl.

• The Korean Journal of Mycology
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• v.19 no.4
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• pp.258-265
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• 1991

Ubiquinone compounds of strains in Leucosporidium scottii, Leucosporidium fellii, Leucosporidium lari-marini, and Rhodosporidium fluviale were analyzed by the high performance liquid chromatography. The Q-9 or Q-l0 compounds, independent on mating or self-sporulating type, were determined in the analyses of Leucosporidium scottii strains. Particularly, there is heterogenous in the quinone compounds in the same strain of L. scottii. These results showed reassessment of the quinone compounds as a taxonomic criterion; L. fellii had the Q-9 compound, L. lari-marini the Q-8 compound, and R. fluviale the Q-10 compound, Taxonomic position of L. lari-marini should be elucidated on the basis of analysis of Q-compound with other aspects.

• The Korean Journal of Mycology
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• v.19 no.4
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• pp.253-257
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• 1991

Cellular carbohydrates were hydrolysed and analyzed in the strains of Leucosporidium scottii and its related species of basidiomycetous yeasts by HPLC methods without any derivatization. Xylose was detected from the hydrolyses of the cellular carbohydrates of L. lari-marini, but not from those of three strains of L. antarcticum, L. fellii, and Rhodosporidium fluviales. not also from those of six strains of L. scottii contrary to other data reported. L. antarcticum and L. lari-marini were considered to be placed on the different genus of Cystofilobasidium or Mrakia, as based on the numerical analyses.

• The Korean Journal of Mycology
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• v.41 no.3
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• pp.185-191
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• 2013

Several yeast colonies were isolated from wild flowers collected from East, West and South coast areas of Korea by plating of flower suspensions on the YPD plates containing antibiotics, streptomycin and ampicillin. Polymerase chain reactions (PCR) were performed for the amplification of D1/D2 region of 26S rDNA for those colonies. PCR-amplified nucleotide sequences were compared using BLAST for their identification. As results, 27 yeast strains belonged to 15 species were isolated from wild flowers collected at Donghae, where is located in eastern coast of Korea. Also, 34 strains belonged to 17 species were isolated from wild flowers of Daecheon, where is located in western coast of Korea. In addition, 22 strains belonged to 13 species were isolated from wild flowers collected at Wando, where is located in southern coast of Korea. Among those 45 species isolated from 3 different collection sites, only 4 species including Cryptococcus laurentii, Metschnikowia koreensis, Pseudozyma rugulosa, and Rhodotorula mucilaginosa were found from all 3 different collection sites. And 5 species including Cryptococcus aureus, Cryptococcus flavus, Hanseniaspora uvarum, Pichia guilliermondii, and Rhodosporidium fluviale were overlapped from the at least 2 different collection sites. Other 23 species were found only in a specific collection sites implying that each area has distinctive yeast flora.