• 대한의생명과학회지
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• 제7권3호
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• pp.145-149
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• 2001

The present study was conducted to determine whether local production of neutrophil chemoattractant cytokines preceded the influx of neutrophils 1311owing dermal scald burn injury. In order to inhibit the influx of neutrophils, we induced scald burn injury on dorsal skin of rats (TBSA 15%) and spread Rhubarb (R. undulatum L.) and complex (Rhubarb+Olibanum+Myrrha) on scald skin in rats. 1. Levels of dermal KC, the indicator of skin iuflammation, caused by burn gradually increased 5 hours, 24 hours (p<0.001) and 72 hours (p<0.001) after the burn occurred. After 24 hours it occurs, KC in skin was decreased by both skin spread of Rhubarb (p<0.05) and complex (p<0.05) compared to scald bum group. After 72 hours, KC by skin sp.end of Rhubarb and complex was significantly decreased (p<0.01), and the inhibition effect of KC production by complex is higher than by Rhubarb. 2. KC content in serum was increased (p<0.01) at 24 hours after burn injury and comes down back to the normal level after 72 hours. At 24 hours postbunt both Rhubarb (p<0.05) and complex (p<0.01) skin spread decreased KC in serum. The inhibition effect of KC production by complex skin spread group is higher than by Rhubarb skin spread group. The data suggest that the KC production effects by Rhubarb skin spread group and complex skin spread group are high. Especially the endurance of the effect by complex skin spread group was longer than by Rhubarb skin spread group. The inhibition effect of KC production by complex skin spread group increases more than by Rhubarb skin spread group as time passes.

• 생약학회지
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• 제36권4호통권143호
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• pp.257-262
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• 2005

This study was carried out to obtain the basic information that can be used to index rhubarbs in six regions of China and Korea. The anthraquinone and stilbene contest in various rhubarb produced in th different area were quantitatively analysed by HPLC. The average of sennoside A content of the Chinghai rhubarb was higher than that of the other palmata rhubarbs(high-quality rhubarbs) produced in China. As a result, the order of the sennoside A content was 1) Chinghai rhubarb, 2) Tsuchan rhubarb, 3)Kansu rhubarb 4) Neimenggu rhubarb. On the other hand, the total stilbenes content of each of the cultivated Korean rhubarb(Rheum undulatum) were higher than that of the Chinese rhapontica rhubarb(low-quality rhubarbs).

• 한국가정과학회지
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• 제8권4호
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• pp.5-12
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• 2005

The Purpose of this study is to investigate dyeing properties of fabrics with rhubarb colorants. Rhubarb colorants were extracted with water from sliced dry root of rhubarb. Using rhubarb extract, wool fabric specimens were dyed under various conditions(temperature, time, pH, mordants). Effects of dyeing conditions and mordanting on dyeing properties and colorfastness were studied. The color of the dyed fabric specimens were predominantly yellow for all of the pH range. The optimum condition for dyeing with rhubarb extract was dyeing at $80^{\circ}C$ for 30minutes. Al of 4 kinds of mordating agents and pre-mordanting showed the highest chroma value. Washfastness was 3-4, but the dyed wool fabrics became darker on light exposure.

• 약학회지
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• 제43권2호
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• pp.233-236
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• 1999

In order to clarify the anti-Ohyul activity of rhubarb, we investigated the effects of water extract from rhizomes of four different rhubarb on blood platelet aggregation induced by arachidonic acid, ADP, collagen and PAF in vitro. The cultivated Korean rhubarb rhizomes (Rheum undulatum) exhibited the most potent inhibitory action on the aggregation induced by arachidonic acid and also among the four fractions, stilbene components containing part showed strong inhibitory action. These inhibitory effect may partially contributed to anti-Ohyul activity of rhubarb.

• 동의생리병리학회지
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• 제18권1호
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• pp.200-205
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• 2004

Uterine leiomyoma is the most common tumor in the female genital tract. Although the tumor is benign, it is of paramount importance since it often causes profuse menstrual bleeding, pressure symptoms, and infertility. Nevertheless, the etiology and patholphysiology of this abnormality remain poorly understood. The traditional definitive treatment for uterine leiomyomas is hysterectomy and, even today, symptomatic leiomyomas are the leading cause of hysterectomy in Korea. Clearly, the development of a safe, effective, and nonsurgical method of treatment for leiomyoma would be of great benefit to many women. The present study was designed to investigate the effect of Rhubarb on apoptosis in uterine leiomyoma cells. Results demonstrate that Rhubarb inhibited cell growth in dose-dependent manner. Cell growth significantly decreased to 60% of control in the treatment of Rhubarb (300㎍/㎖). Associated with the decreased response, there was a concomitant and significant delay of subG1 8.32% above baseline in the treatment of Rhubarb (300㎍/㎖). The delay of subG1 showed a dose-dependent manner, as evidenced by the flow cytometry. The reduced cellular viability on exposure to Rhubarb may represent the induction of apoptosis, at least in part, as concomitantly evidenced by enhanced DNA fragmentation, PARP cleavage and caspase 9 and decreased pro-caspase 3. In addition, Rhubarb decreased clAP1 expression levels in dose-dependent manner. Talcen together, there results suggest that Rhubarb can produce a potent inhibition effect of apoptosis and implicate the delay of G1 phase in the cell cycle and pathways of caspase 3 and 9 in the mechanism underlying inhibitory apoptosis effect of Rhubarb.

• 대한본초학회지
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• 제31권3호
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• pp.37-41
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• 2016

Objectives : The purpose of this study is to assume the size of sliced piece, daily dose and extracting Method of Rhubarb in Treatise on Cold Damage Diseases.Methods : I contrast results of recent studies with assuming results based on original text of Treatise on Cold Damage Diseases.Results : Daily dose was 6, 4 or 2 Ryang in case of cutting Rhubarb in bean-size. These prescriptions were decocted with water or sinked in boiled water. Another daily doses were large baduk-piece size 6 units and baduk-piece size 6 units in case of cutting Rhubarb in size bigger than bean. The former was used in adding to the Jisilchijasi-tang in case of constipation, the latter was used in Sihogayonggolmoryeo-tang and Jeodang-tang. The size of large baduk-piece was 2.32 cm in width, 4.64 cm in length, 4.3 g in weight, and the length and weight of baduk-piece was half of that was. Two sizes of Rhubarbs were sunk in water for 12 hours. After decocting the other ingredients, mixed Rhubarb extraction and Rhubarb, and then boiled it for 1 minute.Conclusions : From this study, daily dose of Rhubarb was 6, 4 or 2 Ryang and the 6 pieces of large baduk-piece or baduk-piece are respectively 4 or 2 Ryang. The extracting methods was decocting, sinking in boiled water for short time, sinking in water for long time and then mixing these with other decocted solution.

• 한국균학회지
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• 제49권4호
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• pp.555-560
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• 2021

During disease surveys conducted from 2019 to 2021 in Korea, leaf spot symptoms in rhubarb (Rheum rhabarbarum) plants were frequently observed in plants growing in fields located in Cheolwon, Taebaek, and Inje in Gangwon Province. The incidence of diseased leaves of the plants in the fields ranged from 2% to 80%. Nine single-spore isolates of Phoma sp. were obtained from lesions of the diseased leaves; all of them were identified as Didymella rhei based on their cultural, morphological, and molecular characteristics. Three isolates of D. rhei were tested for pathogenicity on rhubarb leaves using artificial inoculation. All the tested isolates caused leaf spot symptoms in the inoculated plants; the symptoms were similar to those observed in plants from the fields investigated. This is the first report of D. rhei causing leaf spot in rhubarb in Korea.

• Archives of Pharmacal Research
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• 제22권4호
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• pp.401-403
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• 1999

In continued studies on cultivated Korean rhubarb rhizomes (Rheum undulatum), three known stillbenes (desoxyrhapontigenin, rhapontigenin, piceatannol) have been screened for activity on blood platelet aggregation. Both rhapontigenin and desoxyrhapontigenin exhibited strong inhibition on the aggregation induced by arachidonic acid collagen. However, piceatannol did not show inhibition. These inhibitory effects may partially contribute to anti-blood stagnancy activity of rhubarb.

• 대한본초학회지
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• 제25권4호
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• pp.1-6
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• 2010

Objective : The purpose of this study is to compare anti-thrombotic activities of 4 Rhubarb species. Methods : Rhubarb has been used as medical and edible resources worldwide for the therapy of cancer, constipation and inflammation, etc. To compare the anti-thrombotic activities among the species within rhubarb, we investigated and compared the inhibitory properties of water extracts from R. palmatum(RPE), R. officinale(ROE), R. nobile(RNE), and R. franzenbachii (RFE) on the fibrinolysis, blood coagulation and platelet aggregation. APTT and PT, blood coagulation time, were measured by apparatus of auto blood coagulation analysis that called ACL-7000. The measurement of fibrinolysis was measured and compares with the width of clear zone from melted fibrin plate. The platelet aggregation was measured and compared with inhibition rate of aggregation. Results : We found out the best species from rhubarb showing the significant fibrinolytic, anti-coagulant and anti-platelet aggregation activity. Our results show that Rheum nobile (RNE) has the most effective anti-thrombotic activity among 4 tested rhubarb. Conclusions : This study, therefore, RNE will be able to be used the development of drug which is thrombosis treatment.

• 한국치위생학회지
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• 제23권4호
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• pp.219-226
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• 2023

연구목적: 이 연구는 대황 추출물이 골수 유래 대식세포(BMM)에서 파골세포 분화에 미치는 영향을 조사하는 것을 목적으로 한다. 파골 세포는 골 재흡수 및 재형성에 중요한 역할을 하며, 파골 세포의 조절 장애는 다양한 골 관련 질환을 유발할 수 있다. 잠재적인 항염증 특성을 가진 약용 식물인 대황은 뼈 대사를 조절하는 것으로 제안되었다. 연구방법: 생후 5주령의 C57BL/6 마우스의 대퇴골과 경골에서 BMM을 분리하고 M-CSF(mouse macrophage colony-stimulating factor) 존재하에 3일간 배양한 후 M-CSF와 파골 세포 분화를 유도하기 위한 핵 인자-κB 리간드(RANKL)의 활성화제를 처리하였다. 연구결과: 대황 추출물로 처리하면 BMM에서 파골 세포 분화가 현저하게 억제되었다. 또한 대황 추출물은 파골세포 형성에 필수적인 유전자인 TRAP(tartrate-resistant acid phosphatase) 및 CTSK(cathepsin K)의 mRNA 발현을 억제하였다. 또한 파골세포 분화에 중요한 전사 인자인 활성화된 T 세포 c1(NFATc1)의 핵 인자의 RANKL 유도 발현을 억제하였다. 결론: 이러한 결과는 대황 추출물이 BMMs에서 파골 세포 형성에 억제 효과가 있음을 나타낸다. 따라서 대황 추출물은 비정상적인 파골 세포 활동과 관련된 뼈 관련 질환의 치료를 위한 유망한 치료제이다. 잠재적인 임상 적용을 완전히 이해하기 위해서는 메커니즘에 대한 추가 연구와 탐색이 필요하다.