• Korean Journal of Organic Agriculture
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• v.18 no.1
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• pp.75-82
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• 2010

The purpose of this study is to investigate an occurrence rate of rice stripe virus disease under different rice transplanting time and different rice cultivars. After final harvest, the yield of rice infected by rice stripe virus was obtained on Wangchal cultivar compared to uninfected rice. The results obtained as following: 1) Yield of rice infected by rice stripe virus was decreased by 64% with reduction of culm length, pancile length, number of spike, number of spikelet, and grain filling compared to uninfected rice, 2) An occurrence rate of rice stripe virus disease under different rice planting season was high as order of May 30 > June 15 > July 1. In additional, to reduce a rice stripe virus disease on higher disease occurrence region and susceptible cultivars, rice planting season will be accepted on and after June 15, and 3) Eighteen cultivars were resistance cultivars such as Hwayeongbyeo, Onnuri, Ilmibyeo, Nampyongbyeo, Dongjin2ho, Hopumbyeo, Hwangkeumnuri, Malgeumi, Saenuri, Pungmi1ho, Haechanmulgyeo, Hwangkeumnodeul, Chilbobyeo, Dongjinbyeo, Younganbyeo, Junambyeo, Samkwangbyeo, and Nakdongbyeo. Twenty-one cultivars were susceptible cultivars such as Odaebyeo, Unkwangbyeo, Shinungbong1ho, Manabyeo, Hopyongbyeo, Borami, Damibyeo, Hwangchalbyeo, Sulgengbyeo, Nongrim6ho, Sasanishigi, Yunishigari, Chungmubyeo, Dongjin1ho, Huknambyeo, Guromy, Shanghehanghulna, Heukchalbyeo, Heukhwangbyeo, and Aranghwangchalbyeo.

• Korean journal of applied entomology
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• v.10 no.2
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• pp.85-89
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• 1971

An investigation was carried out to know the host range of the rice stripe virus as a basis of the disease control. 1. In order to investigate the host range of the rice stripe virus 30 species including gramineaceae and forage crop and weeds were inoculated with viruliferous Laodelphax sriatellus Fallen. As a result, 21 out of 30 species showed typical symptoms. 2. Cyperus amuricus Maximowicz var. laxus., Pycreus sanguinolentus Nees. and Eriocaulon robustius Makino, not belonging to gramineaceae were known to be new host plants of rice stripe virus. The highest infection occurred on Pycreus sanguinolentus grown widly in the wet paddy fold. 3. The number of vectors inoculated on the plants was decreased after 48 hours than that of 24 hours.

• Research in Plant Disease
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• v.10 no.1
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• pp.30-33
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• 2004

Until now, occurrence of rice stripe virus (RSV) is limitted in southern part of Korea. However, recently the occurrence of RSV is increased and spreaded in central part of Korea including Chungcheong and Kyonggi province. It is very difficult to distinguish RSV symptoms on virus symptom physiological damage of rice. We detected RSV viral RNA from infected rice and its insect vector Laodelphax striatellus using specific primer of RSV-polymerase and coat protein gene with reverse transcription (RT)-PCR. The result of RT-PCR, we observed specific band including RSV-polymerase (1,,023 bp) and CP (969 bp) in both host of rice and insect vector.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.140.2-141
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• 2003

Among over-wintering small brown planthoppers, population of the rice stripe virus (RSV)-viruliferous insects was surveyed throughout the country in late April of 2003 by using DAS-ELISA. Averaged population of the RSV-viruliferous insects in this year was 2.1％, which was lower than that of last year of 3.7％. However, the insect population in Seoul, Incheon and Kyeonggi areas were relatively high showing 6.7％, 6.2％ and 2.6％, respectively. Based on the survey results, it was expected that overall occurrence of RSV on rice could be decreased in this year, except certain areas. Ovarial transmission rate of RSV by the insects on diseased rice samples collected from 10 areas ranged from 22.2％ to 77.8％. Among 35 graminous weed species collected from rice fields in Ganghwa and Kimpo in 2002 and 2003, common reed and formosens were found to be infected by RSV. The result indicates that those weeds are potential alternative natural hosts of the RSV Further studies on ecological and pathological impacts of the alternative natural host of RSV are being processed.

• The Plant Pathology Journal
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• v.20 no.4
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• pp.313-315
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• 2004

The coat protein gene of Korean isolate of Ricer stripe virus (RSV-Kr) was cloned and its nucleotide sequence was determined. Total RNA was extracted from infected leaves and RSV viral RNA was detected by using RT-PCR with specific primer of coat protein gene. The result of RT-PCR showed a specific band. Purified RT-PCR products of coat protein gene were ligated into the pGEM-T Easy plasmid vector and cloned cDNA was obtained for nucleotide sequence determination. Coat protein gene of RSV-Kr consisted of 969 bp long encoding a protein of 322 amino acids. RSV-Kr showed 94%-99% sequence identities to that of Japanese- and Chinese isolates.

• The Plant Pathology Journal
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• v.29 no.3
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• pp.330-337
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• 2013

Rice stripe virus (RSV), the type member of the genus Tenuivirus, transmits by the feeding behavior of small brown planthopper (SBPH), Laodelphax striatellus. To investigate the interactions between the virus and vector insect, total RNA was extracted from RSV-viruliferous SBPH (RVLS) and non-viruliferous SBPH (NVLS) adults to construct expressed sequence tag databases for comparative transcriptome analysis. Over 30 million bases were sequenced by 454 pyrosequencing to construct 1,538 and 953 of isotigs from the mRNA of RVLS and NVLS, respectively. The gene ontology (GO) analysis demonstrated that both libraries have similar GO structures, however, the gene expression pattern analysis revealed that 17.8% and 16.8% of isotigs were up- and down-regulated significantly in the RVLS, respectively. These RSV-dependently regulated genes possibly have important roles in the physiology of SBPH, transmission of RSV, and RSV and SBPH interaction.

• Korean journal of applied entomology
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• v.54 no.4
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• pp.419-423
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• 2015

Small brown planthopper (SBPH), Laodelphax striatellus, gives a lot of damage to the rice by insect vector of rice stripe virus (RSV). This study compared the RSV acquisition and infection rates according to wing form, sex, and life stage of SBPH. The RSV acquisition rate in macropterous and brachypterous was 60.7% and 63.1%, respectively. The RSV acquisition rate by sex was 61.9% in female and 52.2% in male. However, there was no difference in significance. The RSV acquisition rate of nymphs and adults was 51.2% and 58.7%, respectively. The RSV infection rate by wing form was 53.3.% in macropterous and 48.2% in brachypterous. According to life stage, nymphs was 38.2% and adults was 42.6%. There was no difference in significance. On the other hand, female and male of RSV infection rate was 50.5% and 22.3%, respectively. There was a significant difference. Additionally, developmental periods of SBPH by RSV infection, the longest when inoculated with RSV-infected SBPH in healthy rice, while the shortest when inoculated healthy SBPH in healthy rice.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.12a
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• pp.63-63
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• 2020

Rice stripe virus (RSV) disease is one of the major constraints in rice production, transmitted by the small brown planthopper (SBPH; Laodelphax striatellus). Upon RSV infection, plants develop typical symptoms, which include chlorosis and weakness of newly emerged leaves, white and yellow spots, stripe on leaves, and necrotic and wilting leaves, resulting in plant growth inhibition, oxidative damage that may culminate in programmed cell death (PCD) and plant death in severe epidemics. Although RSV-resistant quantitative trait loci (QTLs), Stv-a, Stv-b, and Stv-bi, were mapped using various resistant varieties, one RSV-resistant gene, OsSOT1, has been identified so far. In this study, we used the rice cultivar Zenith, known to carry Stv-b, to investigate novel RSV-genes through fine mapping. Therefore, we crossed Zenith (Donor parent, RSV resistant) with Ilpum (Recurrent parent, RSV susceptible) to fine-map using a BC₂F₂ population of 2100 plants. Chromosome segment introgression lines that were heterozygous at a different region were selected, two types of heterozygous lines showed an heterozygous genotype between Sid2 and Sid75 to Indel9 and RM6680. Interestingly, we identified qSTV11^Z region harboring Stv-b, covering about 171-kb region between the InDel markers Sid75 and Indel8. The localization of qSTV11^Z provides useful information that could be used for marker-assisted selection and determination of genetic resources in rice breeding.

• The Plant Pathology Journal
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• v.29 no.3
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• pp.223-233
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• 2013

Rice stripe virus (RSV) is one of the most destructive viruses of rice, and greatly reduces rice production in China, Japan, and Korea, where mostly japonica cultivars of rice are grown. RSV is transmitted by the small brown plant-hopper (SBPH) in a persistent and circulative-propagative manner. Several methods have been developed for detection of RSV, which is composed of four single-stranded RNAs that encode seven proteins. Genome sequence data and comparative phylogenetic analysis have been used to identify the origin and diversity of RSV isolates. Several rice varieties resistant to RSV have been selected and QTL analysis and fine mapping have been intensively performed to map RSV resistance loci or genes. RSV genes have been used to generate several genetically modified transgenic rice plants with RSV resistance. Recently, genome-wide transcriptome analyses and deep sequencing have been used to identify mRNAs and small RNAs involved in RSV infection; several rice host factors that interact with RSV proteins have also been identified. In this article, we review the current statues of RSV research and propose integrated approaches for the study of interactions among RSV, rice, and the SBPH.

• Research in Plant Disease
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• v.18 no.3
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• pp.245-249
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• 2012

The small brown planthopper (Laodelphax striatellus) is one of the most important rice pests in Republic of Korea because it damages rice plants not only by sap-sucking but also by transmitting Rice stripe virus (RSV). Outbreaks of RSV are closely related to outbreaks of the small brown planthopper (SBPH). Therefore, it is very important to control SBPH for the management of RSV. Mass-migrating SBPH collected by aerial net traps in June 2011 at Taeanup, Geunheungmyon and Gonammyon in Taeangun were examined for virus carrier status and effects of the pesticide, 'Myungtaja', on the control of RSV. Among 1,217 SBPH trapped, about 7.7% were detected as RSV positive and 4.4% were positive for Rice black streak dwarf virus (RBSDV) by RT-PCR. After the mass migration, pesticide 'Myungtaja' was sprayed once or twice on rice fields and compared to untreated fields. The incidence of RSV was not affected by the frequency of spraying 'Myungtaja' but was influenced by the time of pesticide treatment. Myungtaja' treatment within 5-7 days after mass migration resulted in the most efficient RSV control, resulting in RSV incidence decreased by 87.6% compared to the control. Therefore, we conclude that pesticide spraying for RSV control was most effective when it was done within 5-7 days after mass migration.