• Journal of radiological science and technology
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• v.15 no.1
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• pp.123-130
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• 1992

This study was prepared to observe the change of enzyme activities in kidney treated with red ginseng extract in the gamma ray irradiated mice. Determine the activity of SOD, peroxidase, catalase in the kidney a period of 1 day, 2 day, 3 day, 4 day, 5 day after a saline injection or injection of red ginseng extract or gamma ray irradiatied group into four classify. The activity SOD and catalase showed a tendency to increase and recovery at the early state but pay no regard. Wherease, the activity of peroxide restored and increased pay regard. A physiological saline injection group after gamma ray irradiation showed a tendency to diminish after remakable increase of activity of SOD, peroxidase and catalase than control group. Injection group of red ginseng extract after gamma ray irradiation observed rapid recovery on activity of SOD, peroxidase, catalase than a saline injection group. Experimental result suggested that injection of red ginseng extract after irradiation have the recovery effect on the changed of activity of SOD, peroxidase and catalase against radiation injury.

• Bulletin of the Korean Chemical Society
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• v.22 no.4
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• pp.362-366
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• 2001

To show the effects of metallothionein (MT) on the activity of enzymes involved in the removal of reactive oxygen species, MT has been added to the assay systems of superoxide dismutase (SOD), catalase and peroxidase. We have used assay systems of SOD based on NADPH oxidation and nitrite formation from hydroxylammonium chloride as an assay of superoxide breakdown rate. The two assay systems showed different results at the high concentration of MT. MT showed the scavenging of superoxide in the SOD assay system in the presence and absence of SOD. MT added to the SOD assay system behaved as an activator of SOD, but apo-MT behaved as an inhibitor. When MT was added to the assay system in the presence of a fixed amount of SOD, the breakdown rate of superoxide increased. The effects of MT on the decomposition of hydrogen peroxide and the activity of catalase and peroxidase decomposing hydrogen peroxide were evaluated. MT decreased the activities of catalase and peroxidase. We have concluded that the function of MT as an antioxidant might effect the level of superoxide scavenging and not the level of hydrogen peroxide.

• Toxicological Research
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• v.13 no.4
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• pp.393-400
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• 1997

The effects of superoxide dismutase(SOD) and catalase on the toxicity of paraquat(PQ) were studied using diethyldithiocarbamate(DDC), 3-amino-1,2,4-triazole(AT) which are inhibitors of Cu, Zn-SOD and catalase in rats. Sprague Dawley rats were divide into 6 groups: control, DDC, PQ, AT, DDC+PQ, and AT+PQ group. The PQ (50 mg/kg body weight(BW); about half dose of $LD_{50}$) was administered with orally, otherwise AT(1.0g/kg BW) and DDC(1.0g/kg BW) were administered by intrperitoneal(iP) injection. The survival rate of rats in PQ+AT group was significantly decreased compared with PQ group while the difference of survival rate between DDC group and DDC+PQ group was not significant. The SOD activity after administration of DDC was decreased in liver, lung and kidney, but catalase activity was not changed. The catalase activity in liver, lung and kidney of AT treated rats was decreased, while SOD activity was not changed in this group. The effects of DDC and AT to the PQ toxicity was also observed in primary cultured rat Skin fibroblasts. The viable cells that was measured with MTT method, was decreased in AT+PQ treated group compared to PQ treated group, but the difference of cell viability between DDC treat group and DDC+PQ treated group was not observed. This result, AT potentlate PQ toxicity while DDC were not affect, suggested that the decreased catalase activity lead to elevation of hydrogen peroxide levels and PQ toxicity may be correlate with the hydrogen peroxide rather than the superoxides.

• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.2
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• pp.356-362
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• 2003

In order to examine the antioxidant activities of SJDBT(Sibjeondaebo-tang), the study was done through measurement of parameters such as LPO(lipidperoxidation), GSH(glutathione), SOD(superoxidation dismutase), catalase, GOT, GPT, ALP. The results were obtained as follows: For the weight changes, the kidney and testis of group given SJDBT showed decrease in the weight compared to the control group, and the left cerebrum, right cerebrum, cerebellum and liver of group given SJDBT showed increase. But the changes were not significant. The left cerebrum and right cerebrum of group given SJDBT showed significant decrease in the content of LPO compared to the control group, and in the activity of SOD and catalase showed significant increase. The cerebellum of group given SJDBT showed significant decrease in the content of LPO and GSH compared to the control group, and in the activity of SOD and catalase showed significant increase. For the changes of LPO, GSH in the liver, the group given SJDBT showed significant decrease in the content of LPO and GSH compared to the control group, and in the activity of catalase showed significant increase. For the changes of LPO in the kidney, the group given SJDBT showed significant decrease in the content of LPO compared to the control group. For the changes of LPO, GSH and the activity of SOD, catalase in the testis, the group given SJDBT showed significant decrease in the content of LPO and GSH compared to the control group, and in the activity of SOD and catalase showed significant increase. From above results, the antioxidant action of SJDBT is effective. And it is expected to be necessary to the study of the mechanism in the antioxidant of SJDBT.

• Korean Journal of Microbiology
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• v.40 no.2
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• pp.94-99
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• 2004

The activities of enzymes that act on oxygen derivatives in Rhodobacter sphaeroides D-230 were investigated under various culture conditions. Intracellular SOD activity from the cells grown in aerobic or anaerobic culture conditions was highest at pH 7.0 and pH 8.0, respectively. On the other hand, extracellular SOD activity was highest at pH 6.0. Catalase activity was highest at neutral pH in both cases. Growth of R. sphaeroides D-230 in aerobic or anaerobic culture conditions was inhibited by methyl viologen. As R. sphaeroides D-230 was cul-tured aerobically, SOD activity was increased about 2-fold by addition of iron ion. But $Mn^+2$ had little effect on the SOD activity of R. sphaeroides D-230 grown in aerobically. NaCN, the inhibitor of Cu$.$Zn-SOD, did not inhibit SOD activity. But, $NaN_3$, the inhibitor of Mn-SOD, inhibited SOD activity in anaerobic cultures con-dition. Therefore, R. sphaeroides D-230 produce Mn-SOD in anaerobic condition, although Fe-Sod is produced in aerobic condition. The activity of catalase was induced by methyl viologen, however, extremely inhibited by NaCN and $NaN_3$.

• Journal of Food Hygiene and Safety
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• v.16 no.1
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• pp.41-47
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• 2001

The purpose of this study was to elucidate the effects of green tea catechins (GTC) on the lipid peroxidation and superoxide dismutase (SOD). GTC showed the high SOD activity, while sitgnificantly inhibited the peroxide value of linoleic acid (93%) and lipid peroxidation (84%) from rat liver microsomal fraction induced by Fe$^{2+}$ascorbate system. The effects of GTC on the SOD and catalase activities, and lipid peroxidation after oral administration were investigated. GTC (50 mg/kg) significantly increased SOD (62%) and catalase activities (75%), while significantly inhibited the lipid peroxidation (52%) of rat liver microsome in a dose-dependent manner. These results suggest that GTC has the antioxidative effect which is rotated to the prevention of aging and cancer.r.

• Journal of Periodontal and Implant Science
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• v.25 no.1
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• pp.167-178
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• 1995

It has been believed that the increased release of free oxygen radicals and their tissue damaging potency might be a contributing factor in the pathogenesis of periodontal disease. Antioxidant enzymes such as superoxide dismutase(SOD) and catalase can protect the tissue damage from the free oxygen radicals($O_2^-,H_2O_2$, and $OH^-$). In order to investigate the SOD- and catalase - activity in the blood plasma and red blood cells(RBCs) of the patients with perodontitis, 19 male periodontitis patients($25{\sim}35$ years old) who had good general health, more than 10 teeth with severely inflamed gingiva, attachment loss more than 6mm and bone loss were selected as periodontitis group, and 13 male volunteers($22{\sim}29$ years old) with good general and periodontal health were selected as normal group. After blood plasma and RBC were separated from peripheral blood of 2ml collected from antecubital vein of each subject, SOD- activity in blood plasma and RBCs was measured by the same method that Paoletti et al. did, and catalase - activity in RBC was measured by the same method that Beers et al, did. The difference of SOD- and catalase - activity between the normal and the periodontitis groups was statistically analyzed by Student t-test with SPSS/PC program.The results were as follows : 1. SOD activity in blood plasma was significantly lower in the periodontitis group($1.986{\pm}0.893$) than in the normal group($3.324{\pm}1.044$)(p<0.05). 2. There was no statistical significance in the difference of SOD- activity in RBCs between the periodontitis group($7.753{\pm}3.206$) and the normal group($8.116{\pm}1.192$)(p$242.8{\pm}45.6$) than in the normal group($280.2{\pm}32.6$)(p

• Preventive Nutrition and Food Science
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• v.4 no.1
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• pp.52-56
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• 1999

This study has examined the effects of Aralia elata Seemann ethanol extract on antioxidant enzyme systems inrats along with benzo($\alpha$) pyrene(B(a)P) administration . The ethanol extract of Aralia elata Seemann (50mg/kg body wt.) was fed to rats for 4 weeks by stomach tubing. The extract administration increased antioxidant activities of glutathione sulfur transferase(GST) comparing to the control. also total superoxide dismutase(SOD) and Cu, Zn-SOD activities were stimulated. Catalase activities were increased by 50% with the extract feeding compared to the control . Combined administration of B($\alpha$)P and the extract increased GST activity in B($\alpha$) P group. Although total SOD acitivity was decreased , Cu, Zn-SOD was greately increased from 0.10unit to 0.18 unit and catalase activity also was increased compared to the group of B($\alpha$) P. GST activity in CLE group was 1.32 unit, increased by 33% comparing to the group CL of 0.99unit. Cu, Zn-SOD and catalase activities in thegroup fed high fat and ethanol extracts were increased by 25% and 39%, respectivley comparing to the group of high fat. In addition , total SOD was decreased but, Cu, Zn-SOD acitivity was increased from 0.09 unit to 0.18unit. Catalase activity was 76.05 unit in the group of B($\alpha$) P and extract comparing to 65.26 units in B($\alpha$)P group. Serum $\alpha$-tocopherol of rat was markedly increased by theextract. Administration of B9$\alpha$)P reduced $\alpha$-tocopherol levels in the serum, on the other hand, lard in the diet increased $\alpha$-tocopherol levels in the serum. The above results indicate that Aralia bud exerts antioxidant functions in vivo against B($\alpha$)P. Further research may be necessary for the identification fo the biologically active material.

• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.2
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• pp.436-442
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• 2003

In order to examine the antioxidant actions of YMJHT, the study was done through measurement of parameters such as LPO(lipidperoxidation), GSH(glutathione), SOD(superoxidation dismutase), catalase, GOT, GPT, ALP, the results were obtained as follows: For the weight changes, in the testis the group given YMJHT showed significant increase compared to the control group. In the left cerebrum, the group given YMJHT showed significant increase compared to the control group on the activities of SOD, catalase. In the right cerebrum, the group given YMJHT showed significant decrease on the content of LPO and showed significant increase on the activity of catalase. In the cerebellum, the group given YMJHT showed significant decrease on the content of LPO and showed significant increase on the activities of SOD, catalase. In the liver, the group given YMJHT showed significant decrease on the content of GSH and showed significant increase on the activity of SOD. In the kidney, the group given YMJHT showed significant decrease on the contents of GSH, GSH and showed significant increase on the activities of SOD, catalase. In the testis, the group given YMJHT showed significant decrease on the contents of LPO, GSH and showed significant increase on the activity of SOD. From above results, the antioxidant action of YMJHT is effective. And it is expected to be necessary to the study of the mechanism in the antioxidant of YMJHT.

• Biomolecules & Therapeutics
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• v.7 no.2
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• pp.121-126
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• 1999

In an attempt to define the effects of hyperbaric oxygen treatment on the lipid peroxidation and oxygen free radical reactions in rats exposed to carbon monoxide, we studied malondialdehyde (MDA) level and activities of catalase and superoxide dismutase in the kidney of the rats exposed to carbon monoxide. Male Sprague-Dawley albino rats weighing 240 to 260 gm were used. Experimental groups consist of Control group (=breathing with air), HBO group (=exposed to hyperbaric oxygen 〔HBO, 3ATA, 100%〕 after air breath), CO group (=exposed to CO〔3,970 ppm〕after air breath), CO-Air group (=exposed to CO after air breath followed by air breath) and CO-HBO group (=exposed to CO after air breath followed HBO treatment). The CO group showed significantly higher MDA level, catalase activity and SOD activity as compared to that of control group. The CO-HBO group showed significantly lower MDA level as compared to that of CO group, and did not show significantly lower catalase activity and SOD activity as compared to that of CO group. These results suggest that the excessive oxygen free radicals is an important determinant in pathogenesis of CO-induced nephrotoxicity and HBO inhibits the lipid peroxidation caused by excessive oxygen free radicals in the kidney of the rats exposed to carbon monoxide.