• Horticultural Science & Technology
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• v.19 no.4
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• pp.555-559
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• 2001

This research was conducted to estimate the chlorophyll contents of chrysanthemum leaves using SPAD-502 chlorophyll meter under different photoperiod and light intensity. Measurements were done at every third leaf intervals starting from the top of the stem to the bottom at harvest time. SPAD value was highest at 10 hours of photoperiods, followed by 13 and 16 hours of photoperiods. In particular, under short day condition, SPAD value was highest in the young leaves below the flower bud and decreased down the leaf profile. Under long day condition, SPAD value reached maximum at leaves between $15-27^{th}$ from the top and decreased to the minimum immediately below the flower bud (youngest leaf). These results may indicate that the younger leaves become strong source of supporting flowers in reproductive stage, while the younger leaves serve as sinks and older leaves support their development in vegetative stage. Changes of SPAD value at different levels of irradiance showed that highest SPAD value were observed at high irradiance and decreased with decreasing irradiance.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.3
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• pp.263-267
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• 2002

This experiment was conducted to find out the varietal differences in the relationships of chlorophyll and nitrogen concentration with leaf greenness value (SPAD value). We used two rice varieties with different leaf color type: Hwaseongbyeo has pale green leaves, and Gwanganbyeo has dark green leaves. SPAD value was correlated with chlorophyll a concentration significantly in both varieties, but not with chlorophyll b in Hwaseongbyeo. The correlation coefficients between SPAD value and nitrogen concentration of rice flag leaves were highly significant. However, the regression analysis between SPAD value and nitrogen concentration of rice flag leaves showed different relation among varieties. The slope of the regression line in Gwanganbyeo was steeper than that in Hwaseongbyeo. In conclusion, SPAD value can be applied to estimate the chlorophyll a concentration without consideration of varietal differences. Chlorophyll b and nitrogen concentration in the leaf were regressed to the SPAD values differently with varieties. This implies that the SPAD value can not be used directly to estimate chlorophyll b and nitrogen concentration without consideration of varietal differences.

• Journal of Biosystems Engineering
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• v.28 no.3
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• pp.245-252
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• 2003

This study was investigated the possibility of measuring chlorophyll in paddy crops using two kinds of chlorophyll meters(SPAD-502, Field Scout). The results of correlation analysis showed the 0.7758(r$^2$) relationship between the chlorophyll contents of paddy leaves measured by SPAD and spectrophotometer. It indicates that chlorophyll content in paddy crops could be measured using the SPAD. Considering the data of the SPAD and Field Scout there was significant difference between their chlorophyll contents measured by the SPAD and Field Scout. Likewise, such results were consistent with the corrected data using light intensity. According to the results, it can be concluded that it is difficult to describe the rice chlorophyll measured by the Field Scout more accurately than the SPAD when using one standard spectrum and another spectrum for reflectance measuring. The chlorophyll variance measured at a research institute and a farmer's field revealed that the output of SPAD was more reliable than that of the Field Scout.

• Proceedings of the Korea Water Resources Association Conference
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• 2008.05a
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• pp.805-809
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• 2008

Measurement of leaf area index (LAI) is useful for understanding rice growth, water use, and canopy light interception. The top nitrogen content(TNC) per unit area is an important quantitative index of the condition of nitrogen nutrition in rice production. The rapid and simple method of estimation of TNC, with the use of the existing nondestructive analyzing instruments chlorophyll meter SPAD-502 and plant canopy analyzer (PCA) LAI-2000, was scrutinized. Destructive measurement is time consuming and labor intensive. Our objective was to evaluate sampling procedures using the Li-Cor LI-1800, LAI 2000 plant canopy analyzer (PCA) for nondestructive estimation of rice LAI, and SPAD-502 on the Northern Plains of Cheongju. The LAI estimated by PCA tended to underestimate the LAI determined by actual measurement by about 20%. The estimation of LAI by PCA was judged to have a sufficient accuracy as a practical technique. A high positive correlation was obtained between the values of the SPAD reading and LAI. NDVI and LAI also showed a very high correlation. The values of the SPAD reading and LAI, and NDVI gave a high positive correlation. These results indicated that the method described in this study was effective as a simple and rapid method for the estimation of rice growth.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.68-71
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• 2002

SPAD(Sulfur Particle Autotrophic Denitrification) process is a biological denitrification process which uses elemental sulfur as an electron donor and a mall amount of organic to assist autotrophic denitrification. $^{1)}$SPAD process was applied to a nitrate containing wastewater (200-300mg $NO_3\;^-$ -N/L) with high concentration of $Ca^{2+}$ ion(5000-15000mg/L) from S. Steel Co. in Ulsan city, to est the feasibility of SPAD process. This pilot was operated from November 2001 to early March 2002, and the inner temperature of the pilot was controlled around $20^{\circ}C$. In spite of low temperature, denitrification efficiency was maintained above 90% achieving the average effluent $NO_3\;^-N$ concentration around 20mg$NO_3\;^-$ -N/L.

• Journal of Biosystems Engineering
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• v.35 no.2
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• pp.132-137
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• 2010

Site-specific N application for corn is one of the precision crop management. To implement the site-specific N application, various nitrogen stress sensing methods, including aerial image, tissue analysis, soil sampling analysis, and SPAD meter readings, have been used. Use of side-dressing, an efficient nitrogen application method than a uniform application in either late fall or early spring, relies mainly on the capability of nitrogen deficiency detection. This paper presents map-based variable rate nitrogen application based using a multi-spectral corn nitrogen deficiency(CND) sensor. This sensor assess the nitrogen stress by means of the estimated SPAD reading calculated from the corn leave reflectance. The estimated SPAD value from the CND sensor system and location information form DGPS of each field block was combined into the field map using a ArcView program. Then this map was converted into a raster file for a map-based variable rate application software. The relative SPAD (RSPAD = SPAD over reference SPAD) was investigated 2 weeks after the treatments. The results showed that the map-based variable rate application system was feasible.

• Journal of the Korean Society for Railway
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• v.11 no.1
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• pp.75-80
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• 2008

Human factors still play a significant part in many railway accidents. It is well blown that SPAD (Signal Passed at Danger) remains as the single most cause of railway accidents. In this study a stochastic model is developed to quantify SPAD human reliability. This model provides closed-form mathematical expressions into which multiple factors affecting the reliability of man-machine systems can be incorporated. Two basic elements are combined to form the framework for modeling: random signal occurrence and transient human performance characteristics.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.3
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• pp.224-231
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• 2003

This study was conducted to investigate the varietal differences in leaf senescence during ripening stage and its relation to grain yield of rice. During grain filling period leaf senescence was evaluated by SPAD readings (an indirect indicator of chlorophyll content) for 74 varieties including local, improved domestic, and introduced varieties in the field condition. Leaf senescence was varied greatly among 74 varieties. Jodongji and Dadajo known as local rice varieties had significantly lower SPAD value than the other varieties and became senescent rapidly. However, SPAD value of the flag leaf and 2nd leaf of SNU-SG1 were much higher than the other varieties and leaves of SNU-SG1 also showed a tendency of delayed senescence compared to the other varieties. There were significantly positive correlation between cumulated SPAD value of upper leaf(flag leaf and 2nd leaf) during 35 days after heading and grain yield divided by sunshine hour during 40 days of grain filling and compensated for temperature effect, and cumulated SPAD value of the 4th leaf showed negative correlation with the yield. That is, the delayed senescence of the upper leaves and the rapid senescence of lower leaves were positively associated with grain yield increase.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.6
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• pp.479-483
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• 2003

Knowledge of N accumulation during the vegetative growth stage of rice (Oryza sativa L.) is useful for determining the need for topdressing fertilizer N at panicle initiation and booting stage. The chlorophyll content showing SPAD threshold values generated by a simple and portable diagnostic instrument, chlorophyll meter, enables implement the measures of greenness and/or relative chlorophyll contents of leaves. Two-year field experiment was conducted to evaluate the possible application of the chlorophyll meter for N diagnosis in transplanted paddy rice. The chlorophyll meter threshold values (SPAD reading) were taken from uppermost fully expanded leaves at 10days interval from 10 days after transplanting. SPAD readings and N contents of rice leaf blade were closely related at the maximum tillering stage. SPAD readings during growth period showed M-shaped distribution, sharply finished to drop at 20 days after heading date. As N fertilizer increased, the SPAD readings increased. The SPAD readings showed more critical and clear difference in immature paddy field than in ordinary paddy field, where the former soil showed higher response to N fertilizer topdressed. Based on the PAD readings for obtaining an optimum sink size bearing the maximum grain yield, N fertilizer to be dressed at the panicle initiation stage is to be adjusted within the SPA$\times$tillers value range 888 in ordinary paddy, and 800 in immature paddy vice. Among the cultivars tested, Huckhyangbyeo, Jinpumbyeo, Ansungbyeo, Sobibyeo, Manpungbyeo, Sangmibyeo, Jinbongbyeo, showed high SPAD values, whereas the cultivars, Nonghobyeo, Saechuchungbyeo, Hwabongbyeo, Mananbyeo, did low values and others intermediate SPAD threshhold values.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.6
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• pp.759-765
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• 1997

The process of the senescence in Oryza glaberrima Steud. strains and Oryza sativa L. cultivars were examined in terms of SPAD value(chlorophyll content) and photosynthetic rate. SPAD values and photosynthetic rates of flag leaves for 10 genotypes of each species was measured at the 1, 3 and 5 weeks after heading. SPAD values and photosynthetic rates of O. glaberrima strains tended to decrease rapidly after heading. O. sativa cultivars showed slower decrease as compared to O. glaberrima strains, in particular during the period from 1 to 3 weeks after heading. Although there was no significant difference between the two species in the mean value of photosynthetic rate and SPAD value at 1 weeks after heading, O. glaberrima had lower values after 3 weeks after heading. There were significant positive correlation coefficients between the photosynthetic rate and the SPAD value at 1 and 3 weeks after heading for O. glaberrima strains, and at 1 and 5 weeks after heading for O. sativa cultivars. There were significant positive correlation between the decreasing rates of the photosynthetic rates and the decreasing rates of the SPAD values at the period from the 1 to 3 weeks after heading for both species. At the period from 3 to 5 weeks after heading, only O. glaberrima showed a significant correlation between two traits, indicating that rapid decrease in chlorophyll content would affect the photosynthesis in O. glaberrima.