• Title/Summary/Keyword: SPP

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Distribution of Microorganisms in Domestic Museum Environments (국내 박물과 환경에 분포하는 미생물의 분리)

  • Lee Sang-Joon;Lee Jae-Dong;Cha Mi-Sun;Lee Na-Eun;Yoon Soo-Jeong;Cho Hyun-Hok;Kwon Young-Suk
    • Journal of Environmental Science International
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    • v.14 no.8
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    • pp.793-800
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    • 2005
  • We isolated and identified microorganisms from the aerial environment of domestic museums. The fungi, Penicillium spp., Alternaria spp., and Cladosporium spp. were isolated in many museums. It seems that these fungi are related to biological degradation of textile remains. A total of 14 kinds of bacterial strains were isolated: Acinetobacter spp., Pseudomonas spp., Neisseria spp., Alcaligenes spp., Shigella spp., Klebsiella spp., Corynebacterium spp., Aerococcus spp., Bacillus spp., Micrococcus spp., Citrobacter spp., Erwinia spp., Salmonella spp., and Providencia spp. Acinetobacter spp., Pseudomonas spp., Neisseria spp., and Alcaligenes spp. were the predominate bacteria found in samples with a variety of bacteria. This suggests that there is a relationship between bacteria and the damage of textile remains. In the museum, we isolated Alternaria spp, Geotrichum spp., Penicillium spp. Acinetobacter spp., Pseudomonas spp., Alcaligenes spp. from the entrance, exhibit hall and storage, but they were found in smaller number and species in the exhibit cases and paulownia cases. We concluded that paulownia cases were not influenced by the microorganisms because of quality of care provided by the museum staff. Corynebacterium spp., and Bacillus spp. were not detected at the entrance and exhibit hall but were detected in paulownia cases. It is presumed that those bacteria did not flow in from outside, but resulted from contaminants in paulownia cases. In the distribution of microorganisms associated with textile remains, more fungi were detected than bacteria. Acinetobacter spp., Pseudomonas spp., and Neisseria spp., were isolated from silk items. Penicillium spp. and Cladosporium spp. were isolated in the silk and hump items. Aspergillus spp. and Penicillium spp. were isolated from the cotton items. On the other hands, there were no fungi strains in the wool items. Most of the isolated strains from textile remains were aerial microorganisms from the museum environment. These results suggest that textile remains were apt to contaminated by contact with the air.

Analysis of Microbial Diversity in Nuruk Using PCR-DGGE (PCR-DGGE를 이용한 누룩에서의 미생물 다양성 분석)

  • Kwon, Seung-Jik;Sohn, Jae-Hak
    • Journal of Life Science
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    • v.22 no.1
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    • pp.110-116
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    • 2012
  • Nuruk plays a significant role in the flavor and quality of Takju and Yakju, which are produced through saccharification and alcohol fermentation by various microorganisms. In this study, we identified microbial strains isolated from a plate count and PCR-denaturing gradient gel electrophoresis (DGGE) analysis targeting the 16S and 28S rRNA genes, in order to characterize bacterial and fungal diversity in Sansung Nuruk. The numbers of bacteria and fungi in Nuruk were $1.5{\times}10^9$ CFU/g and $2.2{\tims}10^8$ CFU/g, respectively. The 16S rRNA gene sequence indicated that the predominant bacteria in the isolates and PCR-DGGE profile of Nuruk were Kocuria spp., Pantoea spp., Lactobacillus spp., Pediococcus spp., Weissella spp., Staphylococcus spp., endophytic bacterium, uncultured Gamma-proteobacteria, uncultured Cyanobacteria, and Actinobacteria. Dominant bacteria from the PCR-DGGE profile were Pediococcous pentosaceus and uncultured Cyanobacteria. The 28S rRNA gene sequence indicated the predominant fungi in the isolates and PCR-DGGE profile to be Trichomonascus spp. Pichia spp., Torulaspora spp., Wickerhamomyces spp., Sacharomycopsis spp., Lichtheimia spp., Mucor spp., Rhizopus spp. Aspergillus spp., and Cladosporium spp. Dominant fungi from the PCR-DGGE profile were Pichia kudriavzevii and Aspergillus oryzae. The PCR-DGGE technique was used for the first time in this study to assess a microbial community in Nuruk and proved to be an effective protocol for profiling microbial diversity.

Assessment of Plant Growth Promoting Activities of Phosphorus Solubilizing Bacteria

  • Walpola, Buddhi Charana;Song, June-Seob;Yoon, Min-Ho
    • Korean Journal of Soil Science and Fertilizer
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    • v.45 no.1
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    • pp.66-73
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    • 2012
  • Plant growth promoting traits like production of indoleacetic acid (IAA), ammonia, hydrogen cyanide (HCN), siderophore, and like the enzyme activities of catalase, ACC deaminase, cellulase, chitinase and protease were assayed in vitro for twenty one phosphorus solubilizing bacteria isolated from soil isolates. Except SPP-5 and SPP-15 strains, all the other isolated strains produced IAA in various amounts of 10 to $23{\mu}g\;ml^{-1}$. All strains showed positive response for ammonia production and ACC deaminase activity implying that they are capable of growing in a N-free basal medium. Catalase activity was found to be superior in SPP-2, SPP-7, SPP-12 and SPP-17 compared to the other strains tested. HCN production was detected by 15 strains and among them SPP-9, SPP-15, SAph-11, and SAph-24 were found to be strong HCN producers. Except the isolates SPP-10, SPP-12, SPP-13 and SPP-14, all the other isolates produced more than 80% siderophore units. None of the strains showed cellulose and chitinase activity. SAph-8, SAPh-11, SAPh-24 and SPP-15 strains showed 35.84, 50.33, 56.64 and 34.78 U/ml protease activities, respectively. SPP-1, SPP-2, SPP-3, SPP-11, SPP-17, SPP-18, SAph-11 and SAph-24 strains showed positive response for all the tested plant growth promotion traits except cell wall degrading enzyme activities. According to the results, all the tested phosphorus solubilizing isolates could exhibit more than three or four plant growth promoting traits, which may promote plant growth directly or indirectly or synergistically. Therefore, these phosphorus solubilizing strains could be employed as bio-inoculants for agriculture soils.

Mycoflora in Broiler Houses (Broiler양계사내(養鷄舍內)의 진균총(眞菌叢))

  • Choi, Won-pil;Yeo, Sang-geon;Lee, Hun-jun
    • Korean Journal of Veterinary Research
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    • v.24 no.1
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    • pp.36-39
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    • 1984
  • The present survey was undertaken to determine the mycoflora of broiler houses. Attempts were made to isolate and identify fungi in the dust, feed, litter and water from 21 broiler houses. A total of 166 isolates of fungi was identified as yeast spp. (44%), Aspergillus spp. (30.7%), Verticillium spp. (7.2%), Penicillium spp. (3.6%), Paecilomyces spp. (3.6%), Scopulariopsis spp. (3.0%), Cephalosporium spp. (3.0%), Chrysosporium spp. (2.4%), Cladosporium spp. (1.8%) and Absidia spp. (0.6%). Isolated of Aspergillus(A) spp. and Penicillium(P) spp. were identified as A. fumigatus, A. flavus, A. terreus, A. nidulans, A. niger, P. citrinum and P. palitan. Fungal contamination of the broiler houses predominated in the dust. The important point is that must of the isolates were pathogenic fungi.

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Overexpression of the SPP2 gene of saccharomyces cerevisiae and production of antibodiesd to Spp2p

  • Park, Kwang-Hark;Lea, Ho-Zoo;L. Woolford;Kim, Kyung-Hoon
    • Journal of Microbiology
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    • v.33 no.3
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    • pp.201-207
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    • 1995
  • We have previously reported that SPP2 gene product of yeast Saccharomyces cerevisiae is involved in the pre-mRNA splicing. To investigate the rol ein the splicing pathway of the Spp2p protein, the SPP2 gene was overexpressed in Escherichia coli and polyclonal antibodies to Spp2p were generated from rabbits. First, a DNA fragment containing the SPP2 GENE without its promoter was subcloned into an E. coli expression vector, pKK233-3. The resulting recombinant plasmid pBQ14 contained an IPTG inducible tac promoter and the SPP2 structural gene. Overexpression of the SPP2 gene was achieved by additionof 0.1 to 1.0 mM IPTG to a logarithmic culture of E. coli JM103(pBQ14) for 90 min at 37.deg.C. Sequence of N-terminal 15 amino acids of the overproduced protein was well matched to the deduced one from the SPP2 reading frame. Then, polyclonal antibodies were generated from rabbits immunized with gel-purified SppSp protein. These antibodies reacted specifically with the Spp2p protein extracted from yeast cells expressing the SPP2 gene to a great extent. The antibodies could also block the activity of yeast splicing extracts.

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Antagonism of Pseudomonas spp. against to Rhizoctonia solani and Pythium spp. (Pseudomonas spp.의 Rhizoctonia solani 및 Pythium spp. 병원균에 대한 길항작용)

  • 주영규;한정훈
    • Asian Journal of Turfgrass Science
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    • v.8 no.1
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    • pp.47-52
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    • 1994
  • Attempts were made to investigate the antagonistic activity of soil borne microorganisms Pseudomonas spp. and Trichoderma spp. against to the pathogens of turf diseases Rhizoctionia solani spp. and Pythiom spp. in vitro by a dual culture bioassay. Inhibition zone between the edge of the my-celium and the margin of each antagonistic bacteria, Pocudontonas, on potato dextrose agar was measured 3 days after incubation at 28˚C. Psudomonas spp. showed relatively high inhibition of mycelium growth of R. solani AG-i and Pythium spp. which cause brown patch and pythium blight, respectively. Antagonistic fungi Trichodenma spp. also showed effective inhibition against mycelium growth of both pathogens, more proper methods of measuring the inhibition effects were required because of fast growth of Trichodenna hypae. Brown patch and pythium blight both, re-quire most higher rate of fungicide use to control in golf curses in Korea. Application of antagon-istic microorganisms are useful as biological resources an approach to sole environmental contamination.

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The Distribution of Bacterial Flora in Kunsan bay (군산 내만의 해양수에서 분리되는 세균의 분포)

  • Choi, Min-Soon;Shim, Hyun-Bin;Joung, Kyung-Min
    • Journal of fish pathology
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    • v.13 no.1
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    • pp.67-73
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    • 2000
  • This study was carried out to elucidate the preliminary ecosystem of the heterotrophic bacterial flora in Kunsan Bay located in western costal area of Korea. Samples were collected at 5 sampling stations. Among 123 bacterial isolates, 9 genera of bacteria were appeared as follows; Vibrio spp.(44 isolates/35.7%), Pseudomonas spp.(42 isolates/34.1%), Aeromonas spp.(11 isolates/8.9%), Moraxella spp.(9 isolates/7.3%), Enterobacteria spp.(6 isolates/4.8%), Bordetella spp.(3 isolates/2.4%), Alkaligenesis spp.(3 isolates/2.4%), Flavobacterium spp.(2 isolates/1.6%), and Staphylococcus spp.(3 isolates/2.4%) respectively. Total viable heterotrophic bacteria was ranged from $5{\times}10^3$ to $1{\times}10^5$ CFU/ml and the most abundant viable counts of bacterial population were showed at the stations 2 and 3. This result indicates that the coastal area around Kunsan bay is getting to contaminate far more by municipal wastewaters and industrial byproducts. and so the outbreak of the bacterial diseases will be increased in fish farms.

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Occurrence and control of postharvest diseases of apple (사과저장병의 발생 및 방제)

  • Kim, Yong-Ki;Kim, Ryung-Hee;Ryu, Jae-Dang;Ryu, Jae-Gee;Lee, Sang-Yup;Choi, Yong-Chul
    • The Korean Journal of Pesticide Science
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    • v.2 no.2
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    • pp.83-89
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    • 1998
  • The occurrence of postharvest disease of apple was surveyed from 1995 to 1997 in the major apple-producing area in Korea. Ten genera including Alternaria spp., Botryosphaeria dothidea, Botrytis cinerea, Fusarium spp., and Penicillium spp. were isolated from the decayed apple fruits. Of these, B. cinerea, Penicillium spp. and Fusarium spp. were frequently isolated and were highly pathogenic to apple fruits. Optimum temperature of mycelial growth for Penicillium spp. ranged from 10 to $30^{\circ}C$ and that of mycelial growth for B. cinerea and Alternaria spp. ranged from 5 to $30^{\circ}C$. Optimum temperature of sporulation of Penicillium spp. and Alternaria spp. ranged $15{\sim}25^{\circ}C$ and $10{\sim}20^{\circ}C$, respectively. Occurrence of postharvest disease of apple increased in neglecting selection of wounded fruits before storing apples. Most of these fungi causing postharvest diseases such as Penicillium spp, Botrytis cinerea and Alternaria spp. were isolated from healthy fruits sampled at apple orchard. These results suggested that posthavest diseases of apple were originated from apple fruits contaminated from apple orchard and occurred during storage. In addition, five fungicides including prochloraz EC, fenari EC, captan WP, benomyl WP and folpet WP suppressed posthavest diseases when they sprayed on apple fruits at 30 days before harvesting.

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Antibiotic Susceptibility to Isolated Bacteria and Fungi from the Indoor-air (실내 공기중에서 세균 및 진균의 분포와 분리균의 항생물질 감수성)

  • 장명웅;장태호;박인달;김광혁
    • Journal of Life Science
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    • v.8 no.5
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    • pp.537-549
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    • 1998
  • This investigation was performed to isloate and identify the total bacteria, Staphylococcus spp. and fungi from the indoor air exposed for 30 minutes on the blood agar plate at the 27 places in a hospital. Antibiotic susceptibility tests of the isolated bacteria were also studied. The mean numbers of total bacteria, Staphylococci spp. and fungi were 26, 17, and 2 in the summer and 19, 8, and 2 in the winter, respectively. Staphylococcus epidermidis was the most common isolated bacteria, and the next was Staphylococcus aureus, Aerococcus spp., Micrococcus spp., and Bacillus spp. from the indoor-air of hospital. Aspergillus spp., Cephalosporum spp., Curvularia spp., penicillium spp., and Phialophora spp. was frequently isolated from the indoor-air of hospital. The 109 strains of isolated Staphylococcus epidermidis sho-wed resistance to tetracycline(45.0%), methicillin(40.2%), erythromycin(31.2%), and kanamycin(24.8%). The 76 strains of isolated Staphylococcus aureus showed resistance to erythromycin(71.7%), methicillin(63.2%), kanamycin (44.7%), tetracycline(39.5%), and ampicillin(32.9). The 67 strains of isolated Aerococcus spp. showed resistance to erythromycin(26.9%), methicillin(25.4%), kanamycin(22%), and tetracycline(22.4%). The 48 strains of isolated Micrococus spp. showed resistance to tetracycline(27.0%), methicillin(22.9%), erythromycin(22.9%), and kanamycin(20.8%).

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Antimutagenic Characteristics of Lactobacillua spp. and Bifidobacterium spp. against 2-Nitrofluorene (Lactobacillus spp.와 Bifidobacterium spp.에 의한 돌연변이원물질 2-Nitrofluorene에 대한 돌연변이 억제특성)

  • Yoon, Yeong-Ho;Cho, Jung-Geun
    • Journal of Dairy Science and Biotechnology
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    • v.16 no.2
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    • pp.83-89
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    • 1998
  • Studies on the antimutagenicity of Lactobacillus spp. and Bifidobactrium spp. against 2-nitrofluorene have been conducted utilyzing Salmonella typhimurium TA 98 in order to characterize the activity by the starter and non-starter strains. The average antimutagenic activity of Lactobacillus spp. and Bifidobactrium spp. against 2-nitrofluorene was 20.29% and L. plantarum CU 722 revealed the greatest mutation inhibition activity of 50.34%. An intensive antimutagenicity was found in the cell wall and cytoplasm fraction of L. plantarum CU 722 in skim milk culture showing inhibition rate of 34.9% and 24.5% respectively and very low activity remained in cell free broth and in lactic acid. The optimum cultivation time for Lactobacillus spp. and Bifidobacterium spp. to inhibit mutation was 24 hours and the optimum preincubation time of the reaction mixture containing the mutagen, lactic culture and indicator strain was 60 minutes, and the optimum incubation time for the test plates was 48 hours.

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