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Anthelmintic Efficacy of Albendazole and Ivermectin Against Gastrointestinal Nematodes, Trematodes and Cestode in Korean Native Goats (반추수(反芻獸)의 내부기생충(內部寄生蟲)에 대한 Albendazole과 Ivermectin의 구충효과(驅蟲效果))

  • Suh, Myung-deuk
    • Korean Journal of Veterinary Research
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    • v.26 no.2
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    • pp.321-327
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    • 1986
  • The anthelmintic efficacy of Albendazo1e and Ivermectin against gastrointestinal nematodes, trematodes and cestode was tested in naturally infected Korean native goats. Albendazole was medicated at a dose rate of 10mg/kg of body weight orally and Ivermectin was injected at a dose rate of 0.2mg/kg of body weight subcutaneously. The efficacy was measured by faecal examinations on the day 7th and the day 14th :after treatment. The results obtained were summarized as follows : 1. The efficacy of Albendazole against trematodes and cestode was shown 91.7% in Fasciola hepatica, 68.8% in Paramphislomum spp., 66.7% in Eurytrema pancreaticum and 100% in Moniezia expansa(cestode). 2. The efficacy of Ivermectin against trematodes and cestode was shown 38.8% in Fasciola hepalica, 26.1% in Paramphistomum spp., 22,2% in Eurytrema pancreaticum and 100% in Moniezia expansa(cestode). 3. The anthelmintic efficacy of Albendazole against gastrointestinal nematodes was shown 97.7% in Haemonchus contorus and 100% in Oesophagostomum spp., Ostertagia spp., Bunostomum trigonocephalum, Trichostrongylus spp., Strongyloides papillosus and Cooperia spp. 4. The efficacy of Ivermectin against gastrointestinal nematodes was shown 96.6% in Haemonchus contortus, 94.1% in Trichostrongylus spp. and 100% in Oesophagostomum spp., Ostertagia spp., Bunostomum trigonocephalum, Strongyloides papillosus and Cooperia spp.

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Identification of Woods Excavated from the Peat-bed Site of Ilsan Area (일산 신도시 개발지역 토탄층(土炭層) 출토목재의 수종)

  • Park, S.J.;Kang, A.K.
    • Journal of Conservation Science
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    • v.1 no.1 s.1
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    • pp.12-26
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    • 1992
  • Ilsan peat-bed site, newly constructed city area, is located at the northern part of Seoul. This site is situated near the mouth of Han river, which flows through central Korea. Many fossil woods were excavated from this site, and surveyed the wood anatomical features. Totally 90 specimens were identified into 10 species including two roots and five barks which were not identified in detail. Alnus japonica and A. maxmowiczii are the most common and occupied 26. 7%, 34.4% of the total specimen number respectively, and then other species which excavated from Ilsan area are follows : Fraxinus rhynchophylla(18.9%), Lepido-balanus spp.(3.3%), Maackia amurensis(2.2%), Diospyros spp.(2.2%), Juglans mandshrica(1.1%), Prunophora spp.(1.1%), Malus or Pyrus spp.(1.1%), Rhamnus spp.(1.1%). Among the identified species there are fairly some species growing in the central - or northern temperate zone, such as A. maxmowiczii, F. rhynchophylla, Lepidobalanus spp., M. amurnsis, J. mandshurica, Rhumnus spp. Therefore, it can be estimated that the former temperature of Ilsan area was same or slightly cold than today. The species bearing fruit for food as .J, mandshunca, Diospyros spp., Prunophora spp., Mulus or Pyrus spp. were regarded as artificially planting trees, because they do not grow together in the same site.

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Isolation, Identification and Drug Susceptibility of Bacteria from Genital Organs of Slaughter Sows (도축돈의 생식기내 세균분리 동정 및 약제함수성시험)

  • 한영도;김년수;이종오;육심용;정재용;김동훈
    • Korean Journal of Veterinary Service
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    • v.15 no.1
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    • pp.81-88
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    • 1992
  • This study was conducted to determine the microorganisms inhabitating in sow genital organs and their anti-microbial drug susceptibility During the period between February, 1991 and November 1991, 128 sow genital organs were sampled at six abattoirs. Gross pathological examination and bacterial isolation and identification were performed from the genital organ. In addition, antimicrobial drug susceptibility for the major organisms isolated were examined. 1. Among the bateria isolated from normal genital organs, E. coli(30.7%) Stahylococcus spp.(29.4%), Corynebarterium pyogenes(C. pyogenes) (14.7%), Streptococcus spp.(13.3%) were most freqently isolated, whereas the genera of Klebsiella, Actinobacillus, and Serratia were detected less freqently. 2. Among the bacteria isolated from abnormal genital organs, C. pyogenes,(37.7%), Stahylococcus spp.(30.2%), Proteus spp. (26.4%) , Pasteurella spp. (18.9%) , Steptococcus spp. (9.4%) were most freqently isolated whereas the genera of Pseudomonas, Serratia and Klebsiella were detected less freqently. 3. From sow genital organs showing lesion of endometritis and purulent endometritis C. pyogenes were most freqently isolated, the isolation rate being 67.7% and followed by Stahylococcus spp., E. coli, Proteus spp., Steptococcus spp. and Pasteurella spp. in the order. 4. Antimicrobial drug susceptibility of the major organisms showed that all the isolates were susceptible to cephalothin, ampicillin, chloramphenicol and sulfamethoxazole / trimethoprim, but resistant to penicillin and streptomycin.

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Detection of Waterborne Pathogens by PCR-reverse Blot Hybridization

  • Choi, Yeon-Im;Lee, Gyu-Sang;Bang, Hye-Eun;Kim, Jong-Bae;Lee, Hye-Young
    • Biomedical Science Letters
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    • v.16 no.1
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    • pp.10-18
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    • 2010
  • The present study was set to develop comprehensive system for assessing the safety of drinking water using PCR-reverse blot hybridization assay (REBA). The REBA developed in this study can detect waterborne pathogens such as Shigella spp., Salmonella spp., Citrobacter spp., Enterobacter spp., Klebsiella spp., Yersinia spp., Mycobacterium spp., Listeria spp. at the genus level, and Escherichia coli, Citrobacter freundii, Klebsiella pneumoniae, Pseudomonas aeruginosa, Yersinia enterocolitica, Y. pseudotuberculosis, Mycobacterium avium complex, M. marinum, Enterococcus faecalis, and Staphylococcus aureus at the species level, and E. coli O157:H7 at the strain level.

Anti-Helicobacter pylori Activity of Bifidobacterium spp.

  • Bae, Eun-Ah;Kim, Dong-Hyun;Han, Myung-Joo
    • Journal of Microbiology and Biotechnology
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    • v.10 no.4
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    • pp.532-534
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    • 2000
  • The inhibitory effects of different Bifidobacterium spp. on the growth of Helicobacter pylori (HP) were investigated. A significant suppression of HP growth occurred only when HP was inoculated onto a petri dish containing 0.1 mg/ml of Bifidobacterium spp. When HP was separately cultured with B. breve K-110, B. catenulatum K-309, B magnum K-311, B. magnum K-321, and B. cuniculi K-513, the urease activity was also inhibited by these Bifidobacterium spp. Therefore, it appears that these Bifidobacterium spp. excrete a heat-labile inhibitory component for HP growth into the culture medium. Although most organic acids produced by the Bifidobacterium spp. inhibited the growth of HP, the HP growth was not inhibited by the physiological concentrations of organic acids produced in bifidobacteria-cultured media. Accordingly, these results suggest that some Bifidobacterium spp. may produce antibiotic-like compounds (bacteriocins).

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재래식 메주로부터 효모의 분리, 동정 및 배양조건

  • Lee, Jong-Soo;Yi, Sung-Hun;Kwon, Su-Jin;Ahn, Cheol;Yoo, Jin-Young
    • Microbiology and Biotechnology Letters
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    • v.25 no.5
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    • pp.435-441
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    • 1997
  • Forty seven strains of yeast were isolated from traditional Meju and were identified as Saccharomyces spp. (7 strains), Zygosaccharomyces spp. (7 strains), Kluyveromyces spp. and Hansenula spp. (each 5 strains), Rhodotorular spp. (8 strains), Candida spp. (12 strains), Pichia spp. and Debaryomyces spp. from results of their microbiological characteristics. The optimal medium for growth of all the yeasts was YM media and the optimal initial pH of the medium was 6.0. The optimum temperature for growth was 30$circ$ and among them, Sacch. exiguus OE-5, Sacch. cerevisiae OE-16, Sacch. kluyveri C-1 strains were thermotolerant yeasts which could grow at 40$circ$C.

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Identification of a Strain of Babesia Isolated from Korean Cattle (한우(韓牛)로부터 분리(分離)한 바베시아 원충(原蟲)의 동정(同定))

  • Jeon, Yeong
    • Korean Journal of Veterinary Research
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    • v.18 no.1
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    • pp.27-31
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    • 1978
  • In order to identify unknown Babesia spp. which was isolated from Korean cattle, the morphology of Korean strain was compared with that of Babesia bigemina (Kochinda strain) and Babesia spp. (Miyake strain). Immunofluorescent technique was used to identify the serological character of the parasites. The results obtained were summarized as follows: 1. Korean strain was morphologically very similar to Babesia spp.(Miyake strain) which mostly showed parallel-bigeminate forms, while B. bigemina (Kochinda strain) was mostly round and oval forms. 2. By the indirect fluorescent antibody technique: a) Anti-Babesia spp. and Korean Babesia spp. sera showed a higher antibody titers with Babesia spp. (Miyake strain) antigen (1:500) than with B. bigemina (Kochinda strain) antigen (1:50). b) Anti-Babesia bigemina sera showed a lower titer with Babesia spp. antigen (1:50) than with B. bigemina antigen (1:250). 3. On the basis of morphological and serological confirmantions, a Babesia strain isolated from a Korean cattle was very similar, if not identical, to Miyake strain of Babesia spp.

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Conjunctival fungal flora of the clinically normal pony eye : Including Nocardia and Streptomyces species (임상적으로 건강한 조랑말의 결막 진균총 조사 : Nocardia 종 및 Streptomyces 종을 포함하여)

  • Seo, Kang-moon;Bedford, P.G.C.
    • Korean Journal of Veterinary Research
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    • v.38 no.1
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    • pp.217-225
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    • 1998
  • 영국 Hertfordshire의 두지역에서 사육되고 있는 건강한 조랑말의 결막낭내 상존하고 있는 진균총의 분포도를 조사 비교하고자 36두 조랑말의 양안(총72안)의 상 하 결막낭에서 시료를 채취하여 진균을 분리 동정하였다. 총 76.4%의 안구에서 진균이 발견되었으며, 13종이 분리되었다. 전체적으로 가장 많이 분리된 진균은 Aspergillus spp(27.8%), Nocardia spp(25.0%), Cladosporium spp(19.4%), Penicillium spp(9.7%) 및 Penicillium spp(9.7%)순이었다. 1지역에서는 Nocardia spp와 Cladosporium spp가 가장 많이 분리된 반면, 2지역에서는 Aspergillus spp가 가장 많이 분리되었다. 상 하결막낭내 진균분리율의 차이는 상결막낭에서보다는 하결막낭내에서 진균이 유의성 있게 많이 분리되었다(p<0.05). 시료채취시에 사용된 swab의 상태에 따른 비교에서 건조된 swab과 젖은 swab간에는 통계적 유의성이 없었다.

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Research Trend of Cronobacter Species Detection Methods: A Review (Cronobacter Species의 검출에 관한 연구동향: 총설)

  • Kwon, Heejun;Kim, Myunghee
    • The Korean Journal of Food And Nutrition
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    • v.28 no.4
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    • pp.728-736
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    • 2015
  • Cronobacter species (Cronobacter spp.), previously known as Enterobacter sakazakii, are gram negative food borne pathogenic bacteria. They pose a very high risk of infection to neonates and immuno-compromised individuals and can affect the human central nervous system. Consequently, survivors often suffer from severe neurological impairment including hydrocephalus, quadriplegia, and developmental delays. Cronobacter spp. were not only isolated from plant food and products such as cereals, fruits, vegetables, legume products, herbs, and spices but also from animal source foods such as milk, meat, fish, and products made from these foods. Therefore, rapid detection of Cronobacter spp. is essential for food safety. Many detection methods have been developed since the Cronobacter spp. were first reported. However, the development of more rapid, sensitive, and easy-to-use detection methods for the Cronobacter spp. is required. In this review, our aim was to study and compare the available detection methods for Cronobacter spp., including culture-based, molecular biology-based, and immunology-based methods. This study will contribute to the development of new and rapid detection method for Cronobacter spp.

The Prevalence and Control of Spoilage Mold and Yeast in Cheese (치즈에서 부패를 일으키는 효모와 곰팡이의 다양성 및 저감법)

  • Kim, Jong-Hui;Kim, Bu-Min;Jeong, Seok-Geun;Oh, Mi-hwa
    • Journal of Dairy Science and Biotechnology
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    • v.35 no.3
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    • pp.152-161
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    • 2017
  • Cheese is an excellent substrate for yeast and mold growth. These organisms can cause cheese spoilage, resulting in significant food wastage and economic losses. In the context of cheese spoilage, the presence and effects of spoilage or pathogenic bacteria are well documented. In contrast, although yeasts and molds are responsible for much dairy food wastage, only a few studies have examined the diversity of spoilage fungi. This article reviews the spoilage yeasts and molds affecting cheeses in various countries. The diversity and number of fungi present were found to depend on the type of cheese. Important fungi growing on cheese include Candida spp., Galactomyces spp., Debaryomyces spp., Yarrowia spp., Penicillium spp., Aspergillus spp., Cladosporium spp., Geotrichum spp., Mucor spp., and Trichoderma spp.. In addition, several mold spoilage species, such as Aspergillus spp. and Penicillium spp., are able to produce mycotoxins, which may also be toxic to humans. There are many ways to eliminate or reduce toxin levels in foods and feeds. However, the best way to avoid mycotoxins in cheese is to prevent mold contamination since there are limitations to mold degradation or detoxifications in cheese. Chemical preservatives, natural products, and modified atmosphere packaging have been used to prevent or delay mold spoilage and improve product shelf life and food safety.