• 한국축산식품학회지
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• 제24권2호
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• pp.176-181
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• 2004

우유내 Salmonella를 검출하기 위한 Sandwich ELISA의 특이성을 조사하였다. Sandwich ELISA에 사용한 항체들은 OMP 분획을 닭에 면역주사하여 얻은 IgY와 OMP 분획을 gel filtration하여 얻은 분자량 40,000의 OMP를 토끼에 면역 주사하여 얻은 토끼 IgG를 사용하였다. Immnoblot assay에서 IgY는 분자량 6,000의 OMP에 강하게 반응하였으며 토끼 IgG는 분자량 40,000, 35,000과 6,000의 OMP들에 강하게 반응하였다. IgY와 토끼 IgG는 Salmonella typhimurium의 다른 단백질에도 반응하였다. Competitive ELISA에서 IgY가 Salmonella의 두 개 균주에 대해 특이성을 나타냈으며 Eshcherchia coli와 Yersinia enterocolitica에 의하여서는 반응을 나타내지 않았다. 우유에 세균을 첨가하여 실시한 sandwich ELISA에서 Salmonella typhimurium 2균주가 가장 높은 흡광도를 보였다. Salmonella cholerasuis 균주들은 상대적으로 흡광도가 낮았으며 이루 일부 Salmonella cholerasuis 균주들은 비 Salmonella 균주들과 차이가 없었다.

• 대한족부족관절학회지
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• 제2권2호
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• pp.97-101
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• 1998

Primary subacute pyogenic osteomyelitis, or Brodie's abscess has received much attention since its initial documentation in the literature in 1832 by Sir Benjamin Brodie. Brodie's abscess is a localized form of chronic osteomyelitis that occurs most often in the metaphyseal area of the long bones of the lower extremities of young adults, Intermittent pain of long duration is the presenting complaint, along with local tenderness over the affected area. Laboratory evaluation is unrevealing, with a normal white blood cell count and differential count. The erythrocyte sedimentation rate may also be normal. Roentgenogram shows a markedly varied appearance and an abscess may be easily mistaken for various neoplasm. The most common organism cultured from abscess is Staphylococcus species. Treatment includes curettage of the lesion and administration of antibiotics. We present a case report (with a 1-year follow-up period), demonstrating the successful surgical treatment of Brodie's abscess of the distal metaphysis of the left tibia caused by Salmonella cholerasuis in a 33-year-0ld male who had no hemoglobinopathy.

• 대한수의학회지
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• 제36권1호
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• pp.109-118
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• 1996

The Salmonella rfb gene encoding for the biosynthesis of the oligosaccharide-repeating units of the O-antigenic determinants was cloned and sequenced. A set of nucleotide primers(a forward and reverse) was selected to target a defined region of the guanosine diphospho-mannose(GDP-Man) pyrophosphorylase synthase gene : rfbM of Salmonella C serogroup. The primer set was used to develop a PCR-based rapid and specific detection system for Salmonella C1 serogroup. Amplification bands of predicted size(1,422bp) were generated from 11 different Salmonella C1 isolates. The bands were verified to be specific for the C1 serogroup by Southern blot analysis using reference homologous DNA specificity was further confirmed by the lack of reactivity with heterologous DNA derived from non-salmonella members of the family enterobacteriaeceae. A specificity of 100% was deduced along with a very high sensitivity shown by a detection limit of 1fg of a purified DNA template. The isolated DNA sequence was found to be 99.8% homologous to S montevideo but the related primers amplified with the predicted band sizes with all the Salmonella C1 serogroups tested. It is concluded that the PCR protocol based on the rfbM gene from S cholerasuis is optimal fast and specific for the detection of Salmonella C1 serogroup and also the corresponding probe is suitable for rapid detection of all Salmonella C1 serogroup DNA tested. This technology should facilitate the identification of contaminated pig products and for any other products contaminated with the Salmonalla C1 serogroup. The immediate impact of this developed method will be in the area of food safety of pig products with the potential prospect for adaptation to other food inspection technologies.

• 대한수의학회지
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• 제51권2호
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• pp.123-128
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• 2011

Salmonella spp. is an important pathogen to both public and swine industry. The aim of this study was to investigate the distribution of Salmonella serovar and antibiotics susceptibility of the isolates from Korean swine producing systems. A total of 63 (5.28%) Salmonella spp. was isolated from 1,194 samples (977 fecal materials and 67 organ samples). The predominant Salmonella (S.) enterica serotype and serovar was group B (69.8%) and S. Typhimurium (47.6%), S. Derby (20.6%) and S. Heidelberg (1.6%). But S. Cholerasuis which is characterized host specific by septicemia and enteritis to pigs was not isolated. Antimicrobial susceptibility of the isolates varies as follows: Norfloxacine (75%), Ciprofloxacin (67.5%), Amikacin (60%), Colistin (60%), Enrofloxacin (55%). All of isolates were resistant to Erythromycin, Penicillin, Tetracycline and Lincomycin. The results of this study provided useful information regarding antimicrobial susceptibility and resistance patterns to treat salmonellosis and to prevent emergence of multidrug resistance Salmonella.

• 대한수의학회지
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• 제32권2호
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• pp.211-215
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• 1992

Fecal examination for survey of natural prevalence of Balantidium coli was performed on 1080 healthy bred pigs in Korea, and the positive rate was 56.2%. In order to observe if Balantidium coli might be a secondary invader after certain initiation of the intestinal lesions, the piglet groups preinfected with the protozoa experimentally were treated with Salmonella cholerasuis, Trichuris. cold stress, HCI, and immunosuppressive drug respectively, but no relation was found between them. Also the protozoa were not detected from the intestinal lesions spontaneously formed of 107 pigs which were checked in the slaughter house. According to the above results, Balantidium coli is not directly associated with the formation of any lesions in the pig's large intestine.

• 한국동물위생학회지
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• 제15권2호
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• pp.195-202
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• 1992

Fecal Examination for survey of natural prevalence of Balantidium coli was performed on 1,080 healthy bred pigs in Korea, and the positive rate was 56.2%. In order to observe if Balantidium coli might be a secondary invader after certain initiation of the Intestinal lesions, the piglet groups preinfected with the protozoa experimentally were treated with salmonella cholerasuis, Trichuris, cold stress, HCI, and immunosuppressive drug respectively, but no relatios was found between them. Also the protozoa were not deteceted from the intestinal lesions spontaneously formed of 107 pigs which were checked in the slaughter house. According to the above results, Balantidium coli is not directly associated with the formation of any lesions in the pig's intestine.

• 대한미생물학회지
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• 제6권1호
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• pp.21-27
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• 1971

Recent reports confirm that R factors is widespread in Korea among Enterobacteriaceae isolated from humans. However, no reports have been made concerning the incidence of transferable drug resistance in domestic animals in this country. A total of 211 isolates of Escherichia coli, including 94 strains from dogs, 76 strains from pigs, 30 strains from chickens, and 21 strains from cow milk, were examined for drug resistance and distribution of R factors. And, respective two strains of Salmonella E group and Salmonella cholerasuis which were isolated from dogs and pigs, respectively were also examined for the same purposes. Of 211 strains of E. coli isolated, 66.8% were found to be resistant to 8 antibacterial agents such as streptomycin(SM), tetracycline(TC), chloramphenicol(CP), ampicillin sodium(AP), nalidixic acid(NA), gentamicin(GM), and polymyxin B(PX). Among the isolates, 86.2% of the strains from dogs, 70% of the strains from chickens, 43.4% of the strains from pigs, and 28.6% of the strains from milk, respectively, were found to be resistant to the drugs. The following percentage of resistance of E. coli to each individual drugs was encountered: of 94 strains from dogs, AP, 64.9%; SM, 20.2%; NA, 12.8%, CP and PX, 8.5% each; GM, 2.1% each; GM, 2.1%. Among 76 strains from pigs, 42.2% and 2.6% each were resistant to TC, AP and PX, respectively. Among 30 strains from chickens, 43.3% were resistant to SM, TC, AP, respectively, and no strains were resistant to the other drugs. No strains of the isolated from milk were resistant to the drugs, except that 28.6% were resistant to SM and AP, respectively. Of the strains from dogs, multiply resistant strains(56.8%) were more than singly resistant one(43.2%) and sixteen different drug resistant patterns were observed. The most frequently encountered patterns were AP TC AP and SM CP AP NA. Of the isolates from other sources, the most frequently encountered resistant patterns were as follows: TC among the strains from pigs; SM TC AP from chickens; SM AP from milk. Of the resistant strains from dogs, 32% carried R factors and the most common resistance patterns of R factors were AP TC AP and SM TC CP, whereas 35.2% of the resistant strains from pigs carried R factors of which the most common encountered pattern was TC. Of the resistant strains from chickens, 46.9% carried R factors of which the most common patterns were SM TC TC AP and AP, whereas 50% of the resistant strains from milk carried R factors of which the most common pattern was SM. Of 4 strains of Salmonella isolated, no strains were resistant to the drugs, except that only one strain of Salmonella E group isolated from a dog was resistant to AP. The strain did not harbor R factor.