• Title/Summary/Keyword: Sclerotinia sclerotiorum S2

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Sclerotinia Rot in Stringy Stonecrop Caused by Sclerotinia sclerotiorum

  • Wan-Gyu Kim;Hyo-Won Choi;Gyo-Bin Lee;Weon-Dae Cho
    • The Korean Journal of Mycology
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    • v.50 no.2
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    • pp.143-147
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    • 2022
  • Sclerotinia rot symptoms were observed in stringy stonecrop (Sedum sarmentosum) plants growing in vinyl greenhouses in Yeoju and Icheon, Gyeonggi Province, Korea, during disease surveys in spring 2019 and 2020. The initial symptoms were soft rot on stems and leaves at or above the soil line. Furthermore, the symptoms progressed upwards, and the infected plant parts exhibited white to grayish-yellow discoloration. The infestation of diseased plants in the vinyl greenhouses was 1-5% at the two locations examined. Eight isolates of Sclerotinia sp. were obtained from lesions of the diseased plants. The isolates were identified as Sclerotinia sclerotiorum based on their morphological and molecular characteristics. In addition, artificial inoculation tested three isolates of S. sclerotiorum for pathogenicity on stringy stonecrop plants. All the tested isolates caused Sclerotinia rot symptoms in the inoculated plants. The symptoms were similar to those observed in plants from the vinyl greenhouses investigated. This study is the first report of S. sclerotiorum causing Sclerotinia rot in stringy stonecrop.

Occurrence of Sclerotinia Rot in Solanaceous Crops Caused by Sclerotinia spp.

  • Kim, Wan-Gyu;Cho, Weon-Dae
    • Mycobiology
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    • v.31 no.2
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    • pp.113-118
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    • 2003
  • Solanaceous crops grown in greenhouses and fields in Korea were surveyed from 1994 to 2000. Sclerotinia rot most severely occurred up to 60% in potato. Incidence of the disease was as high as 20% at its maximum in tomato and 5% in eggplant, but as low as less than 1% in red pepper. Symptoms of Sclerotinia rot commonly developed on stems of the solanaceous crops but rarely on fruits of eggplant and tomato. A total of 169 isolates of Sclerotinia species was obtained from the diseased solanaceous crops. Out of the isolates, 165 isolates were identified as S. sclerotiorum, and the others as S. minor based on their morphological and cultural characteristics. S. sclerotiorum was isolated from all the solanaceous crops, while S. minor was only isolated from tomato. Eight isolates of S. sclerotiorum and two isolates of S. minor were tested for their pathogenicity to the solanaceous crops by artificial inoculation. All the isolates of the two Sclerotinia spp. induced rot symptoms on stems of the solanaceous crops tested, which were similar to those observed in the fields. The pathogenicity tests revealed that there was no significant difference in the susceptibility of the solanaceous crops to the isolates of S. sclerotiorum. However, in case of S. minor, the potato cultivar Sumi was relatively less susceptible to the pathogen.

Occurrence of Sclerotinia Rot in Cruciferous Crops Caused by Sclerotinia spp.

  • Kim, Wan-Gyu;Cho, Weon-Dae
    • The Plant Pathology Journal
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    • v.19 no.1
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    • pp.69-74
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    • 2003
  • Cruciferous crops grown in greenhouses and fields in Korea were surveyed from 1995 to 2000. Sclerotinia rot most severely occurred up to 30% in cabbage. Incidence of the disease was as high as 20% at its maximum in Chinese cabbage and rape and 10% in radish, but as low as less than 1 or 2% in broccoli and kale. Symptoms of Sclerotinia rot commonly developed on loaves and stems of the crucifers, but rarely on rachises of broccoli. A total of 112 isolates of sclerotinia species was obtained from the diseased crucifers. Out of the isolates, 103 isolates were identified as S. sclerotiorum, and the rest as 5. minor based on their morphological and cultural characteristics. S. sclerotiorum was isolated from all the crucifers, while S. minor was isolated from Chinese cabbage, broccoli, and kale. Six isolates of S. sclerotiorum and three isolates of S. minor were tested for their pathogenicity to the crucifers by artificial inoculation. All the isolates of the two Sclerotinia spp. induced rot symptoms on the plants of the crucifers tested, which were similar to those observed in the fields. The pathogenicity tests revealed that there was no significant difference in the susceptibility of the crucifers to the isolates of S. sclerotiorum. However, in case of S. minor, radish was relatively less susceptible to the pathogen.

Inhibitory Effect of Paraconiothyrium minitans CM2 on Sclerotial Germination of Sclerotinia sclerotiorum and S. minor Causing Sclerotinia Rot of Lettuce (Paraconiothyrium minitans CM2의 상추균핵병균(Sclerotinia sclerotiorum, S. minor) 균핵 발아에 대한 억제 효과)

  • Lee, Sang-Yeob;Kim, Wan-Gyu;Hong, Sung-Kee;Weon, Hang-Yeon;Park, Kyung-Seok
    • The Korean Journal of Mycology
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    • v.39 no.2
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    • pp.131-135
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    • 2011
  • One fungal isolate CM2 parasitic to Sclerotinia sclerotiorum and S. minor causing Sclerotinia rot of lettuce was identified as Paraconiothyrium minitans based on its morphological and molecular characteristics. P. minitans CM2 grew best on PDA with pH 6.5 at $22^{\circ}C$ under alternating cycles of 12 hr near ultraviolet light and 12 hr darkness. Scleroria of S. sclerotiorum and S. minor treated with conidial suspension of P. minitans CM2 did not directly germinate and produced no apothecia.

Characterization of Sclerotinia sclerotiorum Isolated from Paprika

  • Jeon, Young-Jae;Kwon, Hyuk-Woo;Nam, Ji-Sun;Kim, Seong-Hwan
    • Mycobiology
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    • v.34 no.3
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    • pp.154-157
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    • 2006
  • A fungal isolate collected from infected paprika (Capsicum annuum var. grossum) was characterized as Sclerotinia sclerotiorum based on its ability of sclerotium formation, physiological and molecular properties. When the isolate was grown on potato dextrose agar, oatmeal agar, and malt extract agar, it grew most well on PDA. Optimal temperature and pH for its growth were $25^{\circ}C$ and pH 7, respectively. The fungal isolate produced sclerotia on PDA within 10 days, and the color and shape of the sclerotia were similar to those of S. sclerotiorum. The ITS rDNA regions including ITS1 and ITS2 and 5.8S sequences were amplified using ITS1F and ITS4 primers from the genomic DNAs of the paprika isolate and other known pathogenic S. sclerotiorum isolated from different crops in Korea, and their nucleotide sequences were determined. Sequence comparison analysis showed the ITS rDNA of the paprika isolate shares 100% sequence identity with those of S. sclerotiorum isolated from red pepper, lettuce and a S. sclerotiorum isolate registered in GenBank DNA database. Neighbor joining analysis based on the ITS rDNA sequence revealed the paprika isolate has very close phylogenetic relationships with known Sclerotinia sclerotiorum isolates. This is the first report that S. sclerotiorum has been found associated with paprika rot in paprika growing countries.

Biological Control of Perilla Sclerotinia Rot Caused by Sclerotinia sclerotiorum Using Bacillus megaterium N4. (Bacillus megaterium N4에 의한 들깨 균핵병 (Sclerotinia sclerotiorum)의 생물학적 방제)

  • 문병주;김현주;송주희;이광열;백정우;정순재
    • Journal of Life Science
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    • v.14 no.5
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    • pp.761-769
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    • 2004
  • This study was investigated the occurrence of sclerotinia rot caused by Sclerotinia sclerotiorum at the major perilla cultivating area, Gangdong-dong, Gangseo-gu, Busan in 1998. The incidence of this disease ranged from 8.1 to 28.3% at Gangdong-dong area during the growing seasons. Symptoms of the disease initially appeared damping-off of infected stems and soft-rot on the leaves of perilla. Under the relatively high humidity, abundant white mycelia of the pathogen formed on the lesion developed into black sclerotia later and the infected leaves were finally fell down. Sixteen isolates, Sl-S16, isolated from diseased lesions showing typical symptoms, and pathogenicity was tested using mycerlial disks. Among them, S2 isolate showing the most strong pathogenicity was selected and identified as Sclerotinia sclerotiorum on the basis of morphological and cultural characteristics. For biological control, an antagonistic bacteria, N4 isolate which effectively inhibited not only mycelial growth of S2 isolate but also suppress sclerotinia rot on the pot assay, was selected and identified as Bacillus megaterium according to Bergey's manual and API system., Wettable powder type, N4 formulation using B. megaterium N4 isolate was developed and estimated its control effect on perilla crops in a plastic house. As a results, N4 formulation which applied before 3 days inoculation of pathogen was effectually controlled Sclerotinia rot as the control value of 98.0%, was more effective than chemical fungicide, benomyl showing the control value of 78.0%. This is the first report of wettable powder formulation as a biocontrol agent using B. megaterium N4 against Sclerotinia rot caused by S. sclerotiorum on perilla.

Sclerotinia Twig Blight on Trees and Cottony Rot on Fruits of Satsuma Mandarin Caused by Sclerotinia sclerotiorum

  • Song, Jang-Hoon;Koh, Young-Jin
    • The Plant Pathology Journal
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    • v.15 no.4
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    • pp.236-241
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    • 1999
  • Sclerotinia twig blight on trees and cottony rot on fruits of Satsuma mandarin were observed in Cheju, Korea in 1996 and 1997. The causal fungus causing citrus twig blight and cottony rot was identified as Sclerotinia sclerotiorum which had cup-shaped apothecia bearing hyaline and clavate asci and periphyses on sclerotium. Symptoms were produced on twigs, developing young leaves, fully developed new leaves, and fruits 2-9 days after wound inoculation, but only on twigs with young lateral buds and developing young leaves by unwound inoculation. The fungus grew well between 10 and $27^{\circ}$, but optimum temperature was $24^{\circ}$ on potato dextrose agar. Most varieties were highly susceptible to S. sclerotiorum, whereas some varieties such as Ichinan were relatively resistant among twelve Satsuma mandarin varieties in the field. This is the first record of Scledrotinia twig blight on trees and cottony rot on stored fruits of Satsuma mandarin in Korea.

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Occurrence of Sclerotinia Rot in Four Leguminous Crops Caused by Sclerotinia sclerotiorum

  • Kim, Wan-Gyu;Hong, Sung-Kee;Lee, Sang-Yeob
    • The Plant Pathology Journal
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    • v.22 no.1
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    • pp.16-20
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    • 2006
  • Four leguminous crops grown in greenhouses and fields in Korea were surveyed from 2000 through 2002. Sclerotinia rot most severely occurred up to $60\%$ in Phaseolus vulgaris grown in greenhouses but occurred as low as $0-0.5\%$ in that grown in fields. Incidence of the disease in Pisum sativum grown in greenhouses ranged $1-5\%$, and that in Vicia Java and Vigna sinensis grown in fields was $0.8\%$ and $2\%$, respectively. Symptoms of Sclerotinia rot commonly developed on stems and pods of the crops. A total of 59 isolates of Sclerotinia species were obtained from diseased stems and pods of the crops. All of the isolates were identified as Sclerotinia sclerotiorum based on their morphological characteristics. Eight isolates of the fungus were tested for their pathogenicity to four host crops by artificial inoculation. All of the isolates induced rot symptoms on stems of the host crops tested, which were similar to those observed in the fields. The pathogenicity tests revealed that there was no significant difference in the susceptibility to the isolates among the leguminous crops tested This is the first formal report that S. sclerotiorum causes the Sclerotinia' rot of the four leguminous crops in Korea.

Occurrence of Sclerotinia Rot of Leonurus sibiricus Caused by Sclerotinia sclerotiorum (Sclerotinia sclerotiorum에 의한 익모초 균핵병 밭생)

  • Kwon, Jin-Hyeuk;Park, Chang-Seuk
    • Research in Plant Disease
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    • v.8 no.2
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    • pp.113-116
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    • 2002
  • A sclerotinia rot of Leonurus sibiricus caused by Sclerotinia sclerotio겨m occurred severely in farmer's fields at Seosang-myon, Hamyang-gun, Gyeongnam Province, Korea in 2001. The typical symptoms of the disease were that leaves, stems or collar of the infected plants at first became darker green and then watery soft rotted, and white fluffy mycelia formed on the lesion developed into black sclerotia later, Sclerotia on the plant and PDA medium were globose to cylindrical or irregular in shape, and 0.8~10.3$\times$0.8~6.4 m in size. Aphothecia with numerous asci were cup-shape, and 0.6~1.2 cm in diameter, Asci with 8 spores were cylindrical, and 87~246$\times$4.6~21.6 $\mu\textrm{m}$ in size. Ascospores of one cell were hyaline, ellipsoid to ovoid in shape, and 10.2~14.6$\times$4.7~7.2 $\mu\textrm{m}$ in size. The range of temperature for mycelial growth was from 5 to 3$0^{\circ}C$, and the optimum was $25^{\circ}C$. This is the first report on the sclerotinia rot of L. sibiricus caused by Sclerotinia sclerotioum in Korea.

Effect of Temperature on Sclerotia Formation and Viability of Sclerotinia sclerotiorum Causing Sclerotiorum Rot of Cryptotaenia japonica (파드득나물 균핵병균(Sclerotinia sclerotiorum)의 균핵 헝성 및 생존에 미치는 온도의 영향)

  • Chang, Seog-Won;Lee, Han-Bum;Kim, Sung-Kee
    • Research in Plant Disease
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    • v.9 no.1
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    • pp.47-51
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    • 2003
  • Sclerotinia rot of C. japonica caused by Sclerotinia sclerotiorum was observed throught the growing season in the vinylhouse at the Namyangiu, Yangju and Yangpyung areas. The disease was commonly occurred at late autumn after transplanting and continuously increased until spring in next year, The disease was especially severe at the Yangpyung area among three areas. Three isolates of S. sclerotiorum obtained from C. japonica in different geographic areas were evaluated for ability of sclerotia formation and viability under different temperature. Temperature for mycelial growth ranged from 5 to 3$0^{\circ}C$, with optimum temperature at 15~3$0^{\circ}C$. Sclerotia were fewer formed at low temperature, but their size was larger than that at high temperature. Small size of sclerotia was more viable than those of large size at high temperature. Based on the differences in viability of sclerotia, the relative ranks of sclerotia were in order ~2 mm > 3~4 mm > 5 mm~.