• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.196-200
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• 2002

Compositions of four kinds of products inhibit aging and protect many kinds of disease through eliminating. These compositions inhibit growth of harmful bacteria. such as Bacteroides fragilis. Clostridium perfringens. Staphylococcus aureus, Listeria monocytogenes. Vibrio parahaemolyticus etc. Bacteroides fragilis was controlled by mixing SCUTELLARIA BAICALENSIS GEORGE+CRATAEGI FRUCTUS+PAEONIA JAPONICA+SCHIZANDRAE FRUCTUS. SCUTELLARIA BAICALENSIS GEORGE+CRATAEGI FRUCTUS+PAEONIA JAPONICA+CORNI FRUCTUS. etc. and Clostridium perfringens was SCUTELLARIA BAICALENSIS GEORGE+PHELLODENDRI CORTEX+EPIMEDII HERBA+ASTRAGALI RADIX. SCUTELLARIA BAICALENSIS GEORGE+EPIMEDII HERBA +ASTRAGALI RADIX+GLYCYRRHIZAE RADIX.ste. Listeria monocytogenes was NELUMBO NUCLFERA GAERTNER+SCUTELLARIA BAICALENSIS GEORGE+COPTIDIS RHIZOMA+PAEONIA JAPONICA, NELUMBO NUCLFERA GAERTNER+SCUTELLARIA BAICALENSIS GEORGE+COPTIDIS RHIZOMAtSOPHORAE FLOS. etc. and Vibrio parahaemolyticus was ACANTHODANACIS CORTEX +ASTRAGALI RADIX+PHELLODENDRI CORTEX+COPTIDIS RHIZOMA ACANTHODANACIS CORTEX +ASTRAGALI RADIX + PHELLODENDRI CORTEX+PAEONIA JAPONICA. We expected it as a functional food economical and easy type of taking.

• Korean Journal of Plant Resources
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• v.27 no.6
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• pp.635-641
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• 2014

Scutellaria baicalensis $G_{EORGI}$ (Scutellariae Radix) has been used to clear heat and to dry dampness in the stomach or intestines, which manifests as diarrhea or dysenteric disorder. In this study, we investigated the effective preparation of active components in Scutellariae Radix using the methods of solvent extraction and absorption fractionation for the development of new functional food or pharmaceuticals. The marker substances, baicalin, baicalein, wogonoside, and wogonin were directly isolated from the Scutellariae Radix. There chemical structures were elucidated by spectroscopic analysis. The Scutellariae Radix was extracted with hot water. To enhance yield of flavonoids in Scutellariae Radix, the hot water extract was dissolved in ethanol with concentration dependent manner. The precipitates were separated using centrifugal techniques at 10,000 rpm. Supernatant liquid was applied to the HPLC for quantification of major compounds. Separately, the hot water extract was absorbed on Diaion HP-20 resin. And then, the absorbed fraction was eluted with methanol for HPLC. The contents of baicalin, baicalein, wogonoside and wogonin in different treatment methods were analyzed by HPLC. Total amount of four major components were 16.9% in 50% ethanol extract, 21.7% in 70% ethanol extract, 20.5% in 90% ethanol extract, and 39.3% in absorbed fraction of Diaion HP-20 resin. In these results, we found that resin absorption method is suitable for the extraction of enriched flavonoids from Scutellariae Radix.

• Journal of Applied Biological Chemistry
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• v.62 no.1
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• pp.31-38
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• 2019

Scutellaria baicalensis Georgi (Scutellariae Radix) has been widely used as a dietary ingredient and traditional herbal medicine such as diuretic, hyperlipidemia, antibacterial, anti-allergy, anti-inflammatory and anticancer properties. In this study, the isolation of biomarkers or bioactive compounds from complex S. baicalensis extracts represents an essential step for de novo identification and bioactivity assessment. The bioactive fraction consisted of eight compounds which was chromatographed on an analytical high performance liquid chromatography column using two different gradient runs. A simulative replacement of the analytical column with a medium pressure liquid chromatography and open column allowed the determination of gradient profile to allow sufficient separation in the preparative scale. From the optimized method, eight standard compounds have been identified in the fractions. In addition, MS, UV, HRMS detection was provided by ultraperformance liquid chromatographyequadrupole time-of-flight mass spectrometry (UPLC-QTof-MS) of all fractions. Therefore, this scale up procedure was successfully applied to a S. baicalensis extract.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.737-740
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• 2000

Two groups of bacteria, harmful and useful bacteria, coexist in our body. Harmful bacteria and free radicals which cause many diseases and agings could be controlled by not synthetic materials but natural products. 50 kinds of natural products were searched for this purpose, some of them expressed bacterial controlling ability. Clostridium difficle was controlled by Paeonia Japonica and Lycii Fructus, etc., Clostridium perfringens was inhibited by Scutellaria Baicalensis and Astragali Radix, etc., and same way, Listeria monocytogenes; Paeonia Japonica and Scutellaria Baicalensis, E. coli; Scutellaria Baicalensis and Schizandrae Fructus, Staphylococcus aureus; Paeonla Japonica and Scutellaria Baicalensis, Streptococcus mutans; Coptis Rhizoma, respectively. These natural products will play an important role in bio-Industry.

• YAKHAK HOEJI
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• v.51 no.1
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• pp.68-74
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• 2007

Scutellaria Radix, the dried roots of Scutellaria baicalensis Georgi (Labiatae), has been used in oriental traditional medicine for treatment of fever urine disorder, diarrhea, inflammation. Present study was carried out for the gastroprotective effect of chrysin, baicalein, baicalin, wogonin from Scutellaria baicalensis. This reports evaluated antioxidant effect, antibacterial activity against Helicobacter pylori and HCI-ethanol-induced gastric lesion in rats and showed the significant effectiveness. It may be regarded that the antigastritic effects and antibacterial activity of baicalin, baicalein from Scutellaria baicalensis are originated from acid-neutralizing capacity, free radical scavenging effects and the antibacterial activity against Helicobacter pylori.

• YAKHAK HOEJI
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• v.43 no.4
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• pp.429-436
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• 1999

A simple, accurate and reproducible Capillary electrophoresis (CE) assay has been developed for the determination of baicalin, baicalein, wogonin and chrysin in Scutellaria baicalensis. Successful separation of these compounds has been obtained in 35 mM phosphate butter (pH 7.0) using a untreated fused silica capillary ($57{\;}cm{\times}75{\;}{\mutextrm{m}}$ i.d.) at $25^{\circ}C$ with the electric field of 19kV. Baicalin, baicalein wogonin and chrysin was separated and detected at 280 nm 13 min. The detection limits of CE were acceptable compared to HPLC. Reproducibilities of migration time and peak area were 0.66~1.11% (within-run), 2.18~3.38% (between-run) and 3.50~4.55% (within-run), 3.97~4.82%(between-run) at CE. The results indicate that CE could be a promising technique for quality and quantity control analysis of Scutellaria baicalensis as a validation method.

• Journal of the East Asian Society of Dietary Life
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• v.16 no.2
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• pp.199-206
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• 2006

This study was designed to investigate the effect of medicinal herb extract on the antioxidant and antimicrobial activities against Helicobacter pylori, which is known as a ulcerogenic pathogen. The concentration of total phenolic compound of Scutellaria baicalensis(13.14%) was highest among water extracts and that of Ulmus parvifolia(15.12%) was highest among the ethanol extracts. The antioxidant activity of the water extract of Scutellaria baicalensis and of the ethanol extract of Ulmus parvifolia were 91.00% and 65.03%, respectively, in DPPH assay. The antioxidant activity of the water extract of Scutellaria baicalensis and of the ethanol extract of Ulmus parvifolia were 32.90% and 27.70%, respectively, in SOD assay. The antioxidant activity of the water extract of Ulmus parvifolia and of the ethanol extract of Glycyrrhiza uralensis Fischer was 2.15 and 2.17, respectively, in TBARS assay. In disc method, Scutellaria baicalensis showed the highest anti-microbial activity against H. pylori, followed by Glycyrrhiza uralensis Fischer among the water extracts and Glycyrrhiza uralensis Fischer showed the highest anti-microbial activity followed by Radix puerariae among ethanol extract.

• Korean Journal of Plant Resources
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• v.14 no.2
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• pp.148-151
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• 2001

This study was conducted to investigate effect of activated charcoal on growth and yield of Scutelltaria baicalensis G. in different degree of activated charcoal consistency. The results obtained are summarized as follows. Stem length was shown the longest in activated charcoal 30%, and short in control. Also stem diameter was shown the thickest in activated charcoal 30%, and thin in control. Length of main root and thickness of main root were good in activated charcoal 30%. Quality and yield of Scutellaria baicalensis G. were the highest by activated charcoal 30%. Therefore, optimum consistency of activated charcoal was activated charcoal 30%.

• YAKHAK HOEJI
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• v.43 no.1
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• pp.16-22
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• 1999

Scutellaria baicalensis Georgi (Labiatae) has been extensively used in treatment of diarrhea, conjunctivitis, gastritis, enteritis and skin disease. As instructed in old description, the Scutellariae Radix should sometimes be processed before use. To 노디 light on the changes of chemical compositions by processing as well as processing method-activity relationships, Scutellariae Radix was heated at 16$0^{\circ}C$-20$0^{\circ}C$ for 30 min. in furnace or soaked with 20% EtOH (SPE) and boy's urine(SPU), respectively, which are one of processing methods and then heated at 17$0^{\circ}C$ for 30 min. To isolate the chemical components, Scutellariae Radix with/without processing were extracted with EtOH and EtOH extract was fractionated with ether, ethyl acetate and butanol to give respective fractions. Ether and EtOAc fractions obtained from the processed drug with urine (SPU) were subjected to chromatography to obtainsix components, oroxylin A, Wogonin, chrysin, baicalein, baicalein 7-O-$\beta$-D-glucopyranoside and $\beta$-sitosterol 3-O-$\beta$-D-glucopyranoside. All the isolated compounds were identified by means of physicochemical and spectroscopic methods (IR, $^1H-NMR,{\;}^13C-NMR$, Mass). By HPLC determination, the changes of the contents for each isolated components from SPE and SPU samples were observed. It was found that the content of nonglycosidic flavones such as oroxylin A, wogonin, chrysin and baicalein was increased markedly, whereas the content of baicalin and baicalein 7-O-$\beta$-D-glucopyranoside was significantly decreased in both samples as compared with those of Scutellariae Radix. When the sample was soaked with boy's urine, the total amount of nonglycosidic flavones was higher than that of processing with 20% EtOH.

• The Korea Journal of Herbology
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• v.21 no.4
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• pp.189-195
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• 2006

Objectives : It has been reported previously that the roots of Scutellaria baicalensis (known as Huang-Gum in Korean, henceforth referred to as S. baicalensis) could prevent neuronal cell death after global cerebral ischemia. In Genuine Korean medicine, S. baicalensis is known to relieve fever in upper body, and it was thus thought to be able to alleviate deteriorations in brain function. Methods : The protective effects of S. baicalensis against post-stroke memory retardation using 4-vessel occlusion model were examined in the present study. Results : S. baicalensis was shown to significantly alleviate the deficits in learning and memory by increasing the fraction of time spent in the quadrant in which the platform was initially placed ($34.9\;{\pm}\;3.2%$, p < 0.05) compared to that of the ischemia group ($28.0\;{\pm}\;2.5%$). The cytoprotective effect of S. baicalensis on CA1 hippocampal neurons was evaluated by measuring the neuronal cell density. Neuronal cell density in S. baicalensis extracts-treated ischemia group ($138.0\;{\pm}\;13.6\;cells/mm^2$) was significantly increased compared to saline-treated ischemia group ($22.1 \;{\pm}\;9.3\;cells/mm^2$, p < 0.05). In the study of OX-42 immunohistochemistry, S. baicalensis could decrease the micrgial activation in hippocampus after brain ischemia. Conclusion : These results may provide experimental support for the use of S. baicalensis in treating post-stroke memory impairment.