• Archives of Pharmacal Research
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• v.2 no.2
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• pp.133-144
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• 1979

To investigate constituents of Strobilomyces floccopus (Fr.) Karst. and Coprinus comatus (Fr.) S. F. Gray, free and total amino acids of the two mushrooms were quantitatively analyzed by G. L. C. and an amino acid analyzer. Free amino acids were extracted from both mushrooms with ethanol. Fourtenn free amino acids were detected from the ethanol extract of S. floccopus and fifteen free amino acids from C. comatus by G. L. C. And the dry carphopores of both mushrooms were hydrolyzed with hydrochloric acid and then the total protein amino acids were analyzed by A. A. A. Seventeen total amino acids were detected from each acid-hydrolysate of S. floccopus and C. comatus. Lipids were extracted from the carpophores of S. floccopus and saponified with alcoholic potassium hydroxide. The isolated sterols were subjected to G. L. C. and two sterols were detected. The isolated free fatty acids were methylated with diazomethane and subjected to column chromatography and G. L. C. Eleven saturated and nine unsaturated free fatty acids were detected from the carpophores of S. floccopus. The presence of these nutrient components shows that the two mushrooms can be utilized as edible ones.

• Phonetics and Speech Sciences
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• v.12 no.4
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• pp.37-45
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• 2020

This paper quantitatively calculated the speech frequency of the discourse markers 'a', 'eo', and 'eum' and the speech duration of these discourse markers using the Seoul Corpus, a spontaneous speech corpus. The sound durations were confirmed with Praat, the Seoul Corpus was analyzed with Emeditor, and the results were presented by statistical analysis with R. Based on the corpus analysis, the study investigated whether a particular factor is preferred by speakers of particular categories. The most prominent feature of the corpus is that the sound durations of female speakers were longer than those of men when using the 'eum' discourse marker in a final position. In age-related variables, teenagers uttered 'a' more than 'eo' in an initial position when compared to people in their 40s. This study is significant because it has quantitatively analyzed the discourse markers 'a', 'eo', and 'eum' by gender and age. In order to continue the discussion, more precise research should be conducted considering the context. In addition, similarities can be found in "e" and "ma" in Japanese(Watanabe & Ishi, 2000) and 'uh', 'um' in English(Gries, 2013). afterwards, a study to identify commonalities and differences can be predicted by using the cross-linguistic analysis of the discourse.