• Journal of Chest Surgery
• /
• v.19 no.2
• /
• pp.181-187
• /
• 1986

The changes of serum creatine phosphokinase [CPK], serum lactic dehydrogenase [LDH], serum glutamic oxaloacetic transaminase [GOT] and serum glutamic pyruvic transaminase [GPT] were studied in a total of 82 cases who underwent open heart operation under the cardiopulmonary bypass at the Department of Thoracic and Cardiovascular Surgery, Chungnam National University Hospital. The results were as follows 1. The average values of CPK and COT after the operation were reached to the maximal values on the first postoperative day, which were returned to the normal range until the fifth postoperative day. The average values of LDH and CPT after the operation were reached to the maximal values on the first and second postoperative day respectively, which were significantly increased until the seventh postoperative day. 2. In the relationship of the serum enzymes and duration of the extracorporeal circulation, the values on the group over 90 minute of the extracorporeal circulation were more increased than on the group below 90 minute of the extracorporeal circulation. 3. In the relationship of the changes of the serum enzymes and congenital heart diseases and acquired heart diseases, there were no significantly differences in the values between the two groups.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.28 no.1
• /
• pp.205-211
• /
• 1999

Effects of Houttuynia cordata ethanol extracts on lipid metabolism and antioxidant enzymes in Sprague Dawley male rats were investigated. High fat used in the diet mixture included 10% of lard, 1% of cholesterol and 0.25% of sodium cholate. Total serum cholesterol contents of the rats fed Houttuynia cordata extracts were decreased compared to the control. On the other hand, HDL cholesterol contents were increased along with the decrease of athrogenic index. When high fat diet was fed, total serum cholesterol contents were significantly increased(p<0.01) with the athrogenic index increase of four times of the control. With the administration of Houttuynia cordata extract HDL cholesterol was increased by 53% in the high fat diet group. Antioxidant enzymes including GST and catalase activities were increased comparing the control. On the otherhand, the extracts lowered phospholipid(p<0.01), GOT, GPT, Cu,Zn SOD and alkaline phosphatase enzyme activities in the serum which are related to the liver functions. Therefore, the above results suggest that Houttuynia cordata ethanol extracts can help to maintain normal liver functions and help to protect from peroxidative damages caused by excess dietary fat intake.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.28 no.1
• /
• pp.199-204
• /
• 1999

This study has attempted to examine the effect of Artemisia iwayomogi extract on antioxidant and liver function related enzymes in rats fed high fat diet along with B( )P administration. The activities of the serum glutamic oxaloacetic transaminase, glutamic pyruvate transaminase and alkaline phosphatase of the rats fed Artemisia iwayomogi ethanol extract were decreased compared to the control. Similarily, the activities of the enzymes were also decreased when the combination of B( )P and ethanol extracts were administered compared to the group adminstered only B( )P. On the other hand, high fat diet increased the above liver function related enzymes. The activities of antioxidant enzymes including GST, catalase and Cu,Zn SOD were significantly increased by feeding the extracts (p<0.01) in addition to the increase of tocopherol contents in the serum. These results suggest that Artemisia iwayomogi extracts can protect cell membranes from the damages by free radicals or hydroperoxides and further may lead to the protection from cancer risks.

• Journal of Nutrition and Health
• /
• v.31 no.1
• /
• pp.28-35
• /
• 1998

This study was done to investigate the relationship between ultrasonograph fat density (ULFD) using objective measurement and serum enzymes for testing liver function in 101 healthy adults(43 males and 58 females). Average serum enzyme activities in males and females were GOT27.111U/L and 22.46IU/L, GPT 34.06IU/L and 18.501U/L, and ${\gamma}$-GTP 37.67IU/L and 17.201U/L, respectively. Males showed significantly higher activities of GPT and ${\gamma}$-GTP than females. ULFD of the obese group (BMI$\geq$25) was significantly higher than that of the nonobese group. GOT, GPT, and ${\gamma}$-GTP tended to be high in the obese group. GPT and ${\gamma}$-GTP of the high TG group (TG$\geq$170) tended to be markedly high for males, but not for females. GPT was positively correlated with ULFD, body weight , and weight-to-height, ratio, and ${\gamma}$-GTP was positively correlated with body weight, weight-to-height ratio. BNI, and KI. ULFD and ${\gamma}$-GTP were positively correlated with serum TG. These results suggests that , among serum enzymes for testing liver function, GPT has a close relationship with ULFD using objective measurement, while GOT does not. Also , ${\gamma}$-GTP has a close relationship with parameters for obesity.

• Journal of the East Asian Society of Dietary Life
• /
• v.6 no.1
• /
• pp.41-49
• /
• 1996

This research was performed to Investigate the effects of green tea on the lipid composition of serum and liver and the specific activities of antioxidative enzymes. Male Sprague Dawley rats were fed 10% fat diet with lard and fish oil. Powdered green tea was added to the lard and fish oil diet at the level of 0.1% and 1%. After 6 weeks of feeding, serum and liver were obtained from experimental rats. Then we measured the concentration of total cholesterol, HDL-cholesterol and triglyceride. From liver cytosolic fraction, we analized the specific activities of superoxide dismutase, glutathione peroxidase and glutathione S-transferase. The level of total cholesterol and triglyceride were decreased and the ratio of HDL-cholesterol to total cholesterol was increased by the fish oil in the serum. But in the liver, the level of total cholesterol was increased by the fish oil and green tea than the lard. The specific activities of glutathione S-transferase were more increased in the fish oil than the lard. There was not effect of the green tea of daily dose on the lipid composition of serum and liver and the specific activities of antioxidative enzymes in rats.

• Journal of Sasang Constitutional Medicine
• /
• v.22 no.2
• /
• pp.60-69
• /
• 2010

1. Objectives: The purpose of this study was to analyze the serum lipid, digestive enzymes, thyroid hormone of each Sasang type groups which were classified by Sasang medicine specialist. 2. Methode: The subjects were 1084 (male:female=399:685) person with 371 of Taeeum type, 324 of Soeum type, 389 of Soyang type after the Sasang type classification. The blood samples of the 1084 subjects were to analyze the serum lipid(triglyceride, total cholesterol, HDL-cholesterol, LDL-cholesterol), digestive enzymes(lipase, amylase), thyroid hormone(T3, TSH). The general linear model (SPSS 12.0) were used for the analysis. 3. Results: Taeeum type group ($133.50\pm89.84$, $189.41\pm33.11$, $107.29\pm28.66$) showed significantly higher triglyceride, total cholesterol, LDL-cholesterol than other two group. In digestive enzymes(lipase, amylase), we found significant differences in the order of Soeum>Soyang>Taeeum. Soeum type group($116.23\pm21.38$) has lower T3 than Soyang type group ($119.90\pm21.60$) with marginal significance (p=0.066). 4. Conclusions: The Results of present study showed that Taeeum type showed significantly highest in triglyceride, total cholesterol, LDL-cholesterol. Soeum type showed significantly highest in digestive enzymes. Blood sample of each Sasang type group will serve as an important evidence for further study showing that the Sasang typology has biological basis.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.28 no.1
• /
• pp.212-217
• /
• 1999

The effects of nuruk and wheat bran on cholesterol level in serum and activities of free radical metabolizing enzymes were investigated in rats. The rats were fed a diet containing nuruk or wheat bran for one month. Body weight and food intake were measured. Animals were sacrificed after one month. The increased food efficiency ratio throughout whole growth period was observed in the rats fed with either nuruk containing Aspergillus terreus or wheat bran compared with control group on normal diet. In the rats fed with nuruk, hepatic GSH content, glutathione S transferase activity, hepatic cytochrome P 450 content, and aniline hydroxylase activities were generally increased. In the rats fed with nuruk containing other fungi except Aspergillus terreus, xanthine oxidase activity was decreased. The decreased cholesterol level in serum was observed in rats fed with nuruk prepared from Aspergillus terreus and wheat bran. LDL cholesterol level was decreased in rats fed with nuruk prepared with other fungi such as Penicillium sp. and Rhizopus sp. But HDL cholesterol level was increased in all groups fed with nuruk from any fungi and wheat bran. These results suggested that nuruk or wheat bran supplemented diet might exert their effect by decreasing cholesterol level in serum and amount of oxygen free radical level.

• Journal of Nutrition and Health
• /
• v.35 no.2
• /
• pp.183-191
• /
• 2002

The purpose of this study was to investigate the effect of dietary mushroom powder on blood glucose levels, seam lipid levels, glucose 6-phosphtase (G6Pase), thiobarbituric arid reactive substance (TBARS) and glutathione enzymes in diabetic rats treated with streptozotocin (STZ). Four groups of rats (Sprague-Dawley male rats, 180-200 g) were fed as follows: normal rats were fed a control diet (C), diabetic rats were file a control diet (CD), normal fats were fed a mushroom powder diet (M), and diabetic rals were find mushroom powder diet (MD). Diabetes was induced by single injection of streptozotocin (60 mg/kg B.W.). The animals were fed ad libium each of the experimental diets for five weeks. Food and water intake was determined every day. Blood glucose and serum total cholesterol levels were determined every week. After five weeks, the rats were sacrificed and blood glucose, serum total cholesterol, triglyceride levels and glutathione enzymes were measured. HDL-cholesterol levels were analyzed and LDL-cholesterol concentrations were calculated by equation. There was body weight loss in the diabetic rats, but the MD group showed less body weight loss than the CD group. Blood glucose and serum total cholesterol level of the MD group were lower than those of the CD group (p < 0.05). Also, serum total cholesterol of the M group was lower than that of the C group (p < 0.05). But the serum triglyceride level of the diabetic rats (CD and MD) was higher than that of the normal rats (C and M). However, there was no significant difference between the control diet group and the mushroom diet group. Serum HDL-cholesterol levels of the C group and CD group were higher than that of the M group (p < 0.05), and the MD group was not significantly different. But the serum LDL-cholesterol levels of the M group were lower than those of the C group (p < 0.05). Activity of hepatic microsomal G6Pase significantly increased in the CD and MD, reaching levels higher than those of the C and M groups. Hepateic gutathione S-transferase (GST, glutathione reductase (GR) and glutathione peroxidase (GPX) activity was not significant. But renal GST, GR and GPX activity in the MD group was lower than that of the CD group (p < 0.05). These results suggest that dietary mushroom reduces renal disorders such as oxidation and aging of tissue. In conclusion, dietary mushroom groups reduced blood glucose and cholesterol levels in STZ-induced diabetic rats and renal glutathione enzymes activity was averted in diabetic rats.

• Journal of the Korean Applied Science and Technology
• /
• v.1 no.1
• /
• pp.23-31
• /
• 1984

In order to investigate the acute toxicity of autoxidized methyl linoleate(AOML) on the activity of serum enzymes in the mouse, we administered once 0.45ml of AOML to ICR strain mouse by using stomach tube. The following results were obtained: The total lactic dehydrogenase(LDH) activities in the serum of AOML group were generally increased than those of normal group. According to electrophoresis, the activities of LDH, were increased while those of LDH, were decreased. The activities of glutamic oxaloacetic transaminase(GOT), glutamic-pyruvic transaminase(GPT) and ${\alpha}-amylase$ in the serum of AOML group were increased more than those of normal group. The activities of alkaline phosphatase in the serum of AOML group were increased but those of isozyme were not confirmed in the normal and AOML group. In the serum protein of AOML group, albumin was increased, on the other hand ${\gamma}-globulin$ was decreased. At the peripheral blood slide smear, lymphocytes were significantly decreased but neutrophils were increased and the morphological change of erythrocytes was observed. From these results we conclude that the AOML fed to mouse influences on the activity of various serum enzymes and blood cells in the mouse.

• Journal of Microbiology
• /
• v.34 no.2
• /
• pp.198-205
• /
• 1996

Two proteases were partially characterized from culture filtrate of Mycobacterium, tuberculosis KIT110. Their molecular weights were approximately 200 and 180 kDa, respectively and they exhibited similar enzymatic characteristics. These enzymes were inhibited significantly by EDTA and to some extent by EGTA. Their activity was enhanced by $Ca^{2+}$ and $Mg^{2+}$ to some degree. However, $Cu^{2+}$ and $Ag^{2+}$ completely inhibited the enzyme activity at the concentration of 2.5 and 5 mM, respectively. The optimal pH was 7.0 and optimal temperature was around $40^{\circ}C$. These enzymes were rapidly inactivated at $80^{\circ}C$. Therefore, they were heat-labile, neutral metalloproteases. These enzymes exhibited antigenicity shown by their reacting with sera from the partients with pulmonary tuberculosis. These enzymes were able to degrade serum proteins including hemoglobin, bovine serum albumin, lysozyme and immunoglobulin G and structural matrix protein such as type I collagen. Therefore, these enzymes may be thought to contribute to tissue necrosis and pathogenesis during infection.