• Korean Journal of Fisheries and Aquatic Sciences
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• v.42 no.5
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• pp.449-455
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• 2009

In Hansan Geojeman area, 2,050 ha of shellfish growing area has been designated as shellfish production area for export. The main shellfish species from the designated area is oysters. For the sanitary management of the designated area established in Hansan Geojeman area, bacteriological examination of sea water and shellfish at the sampling stations inside and outside of the designated area were performed from January 2006 to December 2008. The range of fecal coliform of 756 sea water samples at 21 stations located in the designated area were <1.8~>1,600 MPN/100mL. And the range of geometric mean and the estimated 90th percentile of fecal coliform were 1.8~2.9 and 2.7~15.8 MPN/100mL, respectively. Sanitary conditions of the current designated area in Hansan Geojeman meets the required standards of the Fisheries Product Quality Control and National Shellfish Sanitation Program (NSSP, USA) criteria for the approved area. Also, the sanitary status of the shellfish harvested from the designated area met the Korean Shellfish Sanitation Program (KSSP) fecal coliform criterion (<230 MPN/100g). And the human pathogen such as Salmonella spp. and Shigella spp. were not detected from the examined shellfish samples.

• Journal of Environmental Science International
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• v.26 no.6
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• pp.691-710
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• 2017

This study was performed to investigate the geochemical and benthic environment of three shellfish farms in Suncheon Bay during the period of September 2014 ~ April 2015. Three sampling stations were selected; St.1 is the shellfish farm of razor clam near Jangsan area. St.2 is the shellfish farm of small ark shell near Hwapo area and St.3 is the shellfish farm of razor clam near Yongdu area. Razor clam was the dominant species at St.1, small ark shell and granulated ark shell were dominant at St.2 and St.3, respectively. Granulated ark shell inhabited St.3, although it is not cultured at that station. This station's exposure to air during the ebb tide and sediment composition likely provides the appropriate habitat for granulated ark shell species. Analysis of the number of different species showed that 8 benthos species were found to be distributed at St.1, 18 species at St.2, and 13 species at St.3. Among three stations, the highest Ignition Loss (IL), Chemical Oxygen Demand (COD) and Acid Volatile Sulfide (AVS) values were obtained from the sediment at St.2. The analysis of pore water from St.2 also showed the highest values of Total Organic Carbon (TOC), ammonia ($NH_4^+$), Dissolved Inorganic Nitrogen (DIN) and phosphate ($PO_4^{3-}$). These results are related to the fact that species dominance and richness is the highest in St.2.

• Fisheries and Aquatic Sciences
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• v.11 no.1
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• pp.7-14
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• 2008

The sanitary quality of a shellfish-growing area in Kamak Bay, Korea, was assessed through a bacteriological examination of seawater and oysters from January 2004 to December 2006. From seawater samples collected at 28 stations, the range of geometric mean (GM) and the estimated 90th percentile for most-probable-number (MPN) values of fecal coliforms were <1.8-2.4 MPN/100 mL and 1.8-6.0 MPN/100 mL, respectively. The observed fecal coliform GM and the estimated 90th percentile did not exceed the fecal coliform water quality standards of 14 MPN/100 mL and 43 MPN/100 mL. Therefore, the bacteriological quality of seawater at this shellfish-growing area met the Korean Shellfish Sanitation Program (KSSP) criteria for a growing area used for export. The range of the fecal coliform GM and the estimated 90th percentile MPN values of oyster samples were 19.2-160 MPN/100 g and 20.2-166.9 MPN/100 g, respectively, and the range of the viable cell count was 30-1900 CFU/g. Thus, the fecal coliform value for the oysters and the viable cell count were less than the current shellfish quality standards of 230 MPN/100 g and 50,000 CFU/g, respectively. The bacteriological quality of the oysters complied with the criteria for domestic use and export of shellfish.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.33 no.6
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• pp.546-549
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• 2000

For the establishment of biodetoxification method which can be acceptable for live bivalves, paralytic shellfish poison (PSP) detoxification bacteria were isolated from sea water and bivalves, and PSP detoxification activity and optimal growth condition of the isolated strains were investigated. from the bivalve and sea water samples, 8 strains of PSP detoxification bacteria were isolated. Of the isolated strains, CW-6 isolated from sea water shown strong PSP detoxification activity and decomposed completely 18 nmole/g of GTX2 after 3 days incubation in artificial medium. The selected stain CW-6 shown typical characteristics of the Enterobacter sp. and identified as Enterobacter sp, CW-6. Optimal growth condition of the Enterobacter sp. CW-6 were $35^{\circ}C$, pH 7 and $NaCl 1{\%}$, respectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.39 no.2
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• pp.59-65
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• 2006

Fluoroquinolones are the most common group of antibacterial agents currently used in the Korean aquaculture industry, and use of these agents has been increasing steadily. High performance liquid chromatography (HPLC) with fluorescence detection was used for the simultaneous determination of five fluoroquinolones in fish and shellfish: ofloxacin (OFL), pefloxacin (PEF), norfloxacin (NOR), ciprofloxacin (CIP), and enrofloxacin (ENRO). Fish and shellfish muscle was homogenized, and protein, lipid, and low molecular weight pigments were then excluded from the homogenate. The final eluates were analyzed by HPLC equipped with a Shiseido UG-120 type C18 reverse-phase column ($4.6{\times}250 mm$, $5{\mu}m$) and a fluorescence detector (excitation at 280 nm, emission at 450 nm). The mobile phase was 0.1 M phosphoric acid and acetonitrile solution (91:9, v/v) and tetrahydrofuran (THF) was added to it at a rate of 5 mL per a liter of the mobile phase. Adequate chromatography separation was obtained using the above method. Average recoveries of fortified samples at levels from 0.05 to 0.5 mg/kg were $72.3{\pm}2.5-84.5{\pm}1.2%$ for OFL, $82.7{\pm}3.3- 109.3{\pm}7.5%$ for NOR, $85.3{\pm}6.6-116.0{\pm}7.9%$ for PEF, $76.0{\pm}4.3-109.3{\pm}12.4%$ for CIP, and $78.7{\pm}5.9-100.0{\pm}9.8%$ for ENRO. The limit of detection of OFL was $5{\mu}g/L$, the others were $1{\mu}g/L$. We concluded that the new analytical method was suitable for the determination of fluoroquinolones in fish and shellfish.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.43 no.1
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• pp.25-32
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• 2010

A Sanitary survey in an Important shellfish-growing area of Biin Bay in Seocheon-gun Korea, was conducted to evaluate bay conditions and compliance with the bacteriological criteria for areas designated for the production of shellfish for export. Seawater samples were collected monthly at 55 sampling stations established in the survey area from January 2006 to December 2008. Bacteriological water quality did not change in response to a small rainfall (10.0 mm), but it increased abruptly in response to rainfall to 62.5 mm The most probable number (MPN) value for total coliforms and fecal coliforms in 1, 980 seawater samples ranged from <1.8 to >1, 600 MPN/100mL and from <1.8 to 330 MPN/100mL, respectively. The geometric mean and estimated $90^{th}$ percentile value for total coliforms ranged from 2.0 to 10.3 MPN/100mL and from 7.0 to 42.6 MPN/100mL respectively. The geometric mean and estimated percentile value for fecal coliforms ranged from 1.8 to 4.0 MPN/100mL and from 1.9 to 18.3 MPN/100mL, respectively. Accordingly, the bacteriological water quality of Biin Bay met the National Shellfish Sanitation Program (NSSP) and Korea Shel1fish Sanitation Program(KSSP) criteria for areas designated for shellfish production for export in Korea.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.41 no.3
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• pp.155-162
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• 2008

The seawater in the Taean area was surveyed to evaluate the conditions of the bay and compliance with the bacteriological criteria for a designated area for shellfish production for export. Samples of seawater were collected monthly at 34 sampling stations established in the survey area from January 2002 to December 2004. The bacterial density in the coastal area close to a pollution source located to the northeast of the survey area was higher than in the open sea to the west. The bacteriological counts in the water did not change with 16.5mm of rainfall, but increased abruptly after 65.4mm of rainfall. The total coliform and fecal coliform most probable numbers (MPNs) of 1,224 seawater samples in the survey area were <1.8-2,400 and <1.8-790 MPN/100 mL, respectively. The geometric mean and estimated 90th percentile of total coliforms were 1.9-3.4 and 2.7-26.3 MPN/100mL, respectively, and for fecal coliforms were <1.8-2.6 and 1.8-12.0 MPN/100mL, respectively. The bacteriological water quality in the Taean seawater area met the National Shellfish Sanitation Program criteria for an approved area and the Korea Shellfish Sanitation Program criteria for a designated area for shellfish production for export.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.43 no.6
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• pp.614-622
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• 2010

The sanitary quality of a shellfish-growing area in Gangjinman, Korea, was assessed through a bacteriological examination of seawater form January 2007 to December 2009. The range of the geometric mean (GM) and the estimated 90th percentile for most-probable-number (MPN) values for fecal coliforms in samples collected in the designated area were 2.0-2.4 and 6.1-11.5 MPN/100 mL, respectively. Therefore, the bacteriological quality of seawater in the designated area in the Gangjinman area met the National Shellfish Sanitation Program (NSSP, USA) guidelines for approved areas. Also, the sanitary status of shellfish harvested from the designated area met the Korean Shellfish Sanitation Program (KSSP) fecal coliform criterion (<230 MPN/100 g). However, the degree of bacteriological contamination increased rapidly, and water quality exceeded the criteria for approved areas when large amounts of rain fell before the sampling date. Fortunately, fecal pollutants did not affect the bacteriological quality of a buffer zone established between the shoreline and the designated area. These results indicate that the current buffer zone established around the designated area protects the designated area properly.

• Applied Biological Chemistry
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• v.50 no.2
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• pp.121-126
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• 2007

Halophyte and shellfish are functional and favorite foods in Korea. This study was conducted to survey the contents of inorganic metals to elucidate seafood hygiene & safety. The average concentrations of heavy metals in the halophyte and shellfish were 1.73, and 1.54 ppb of Cd and 47.37, 25.06ppm of Cu, respectively. Average contents of Hg in halophytes was 0.26 ppb and 50.43 ppb in shellfish. In case of minerals, the average concentrations were 4,475.62, 4,440.54 ppm of Ca, and 3,936.16, 1,227.09 ppm of Fe, 2,145.36, 6.170.60 ppm of P. and 314.53, 162.11 ppm of Zn, respectively. The average values of heavy metals were all below the permissible amounts in food but minerals showed high levels in halophyte and shellfish.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.33 no.6
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• pp.550-553
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• 2000

Optimum temperature for paralytic shellfish poison (PSP) detoxofication of Enterobacter sp. CW-6 isolated from sea water and changes of contents and ingredients composition of PSP during bacterial detoxification process were investigated. Enterobacter sp. CW-6 detoxicated $61.5{\~}67.7{\%}\;and\;87.4{\~}96.8{\%}$ of initial PSP toxicity ($25.0{\~}28.5\;nmole/g$) after $5{\~}12$ days at 30 and $35^{\circ}C$, identified as optimal growth temperature, respectively. The detoxification rate of Enterobacter sp. CW-6 for crude PSP with initial concentration of 38.2 nmole/g after 8 and 12 days at $30^{\circ}C$ in the Marine broth was 88.4 and $92.7{\%}$, respectively. During bacterial detoxification process using crude toxin solution, temporary increasement of STX group was detected and identified that was derived from GTX2, 3 group. The detoxification rate of Enterobaoter sp. CW-6 on purified GTX1 and 4 with initial concentration 47 nmole/g and 37 nmole/g were more than $90{\%}$ after 12 days in the marine broth at $30^{\circ}C$. Enterobacter sp. CW-6 also showed a detoxification activity on purified GTX2 and 3, and the detoxification rate for the initial concentration 25.6 nmole/g after 12 days was $66.4{\%}$.