• Proceedings of the Korean Nutrition Society Conference
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• 1992.05a
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• pp.28-29
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• 1992

• Biomolecules & Therapeutics
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• v.25 no.6
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• pp.586-592
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• 2017

Sinomenium acutum has been long used in the preparations of traditional medicine in Japan, China and Korea for the treatment of various disorders including rheumatism, fever, pulmonary diseases and mood disorders. Recently, it was reported that Sinomenium acutum, has sedative and anxiolytic effects mediated by GABA-ergic systems. These experiments were performed to investigate whether sinomenine (SIN), an alkaloid derived from Sinomenium acutum enhances pentobarbital-induced sleep via ${\gamma}$-aminobutyric acid (GABA)-ergic systems, and modulates sleep architecture in mice. Oral administration of SIN (40 mg/kg) markedly reduced spontaneous locomotor activity, similar to diazepam (a benzodiazepine agonist) in mice. SIN shortened sleep latency, and increased total sleep time in a dose-dependent manner when co-administrated with pentobarbital (42 mg/kg, i.p.). SIN also increased the number of sleeping mice and total sleep time by concomitant administration with the sub-hypnotic dosage of pentobarbital (28 mg/kg, i.p.). SIN reduced the number of sleep-wake cycles, and increased total sleep time and non-rapid eye movement (NREM) sleep. In addition, SIN also increased chloride influx in the primary cultured hypothalamic neuronal cells. Furthermore, protein overexpression of glutamic acid decarboxylase ($GAD_{65/67}$) and $GABA_A$ receptor subunits by western blot were found, being activated by SIN. In conclusion, SIN augments pentobarbital-induced sleeping behaviors through $GABA_A$-ergic systems, and increased NREM sleep. It could be a candidate for the treatment of insomnia.

• The Journal of Korean Medicine
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• v.21 no.2
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• pp.52-59
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• 2000

Objectives: The root and stem of Sinomenium acutum has been used for treatment of arthritis and neuralgia in oriental medicine. To find new substances of the anti-anaphylactic drugs, we studied Sinomenium acutum. Methods: To investigate the effect of this plant, the effect on anaphylactic reaction, plasma histamine level, and tumor necrosis $factor-{\alpha}-(TNF-{\alpha})$ production were measured after the aqueous extract of Sinomenium acutum stem (SSAE) was administrated to mice and rats. Results: The SSAE (0.1 to 1000 mg/kg) dose-dependently inhibited systemic anaphylactic reaction induced by compound 48/80 in mice. Especially, SSAE reduced compound 48/80-induced anaphylactic reaction with 50% at the dose of 1000 mg/kg. SSAE (100 to 1000 mg/kg) also significantly inhibited local anaphylactic reaction activated by anti-dinitrophenyl (DNP) IgE. When mice were pretreated with SSAE at a concentration ranging from 0.1 to 1000 mg/kg, the plasma histamine levels were reduced in a dose-dependent manner. SSAE (1 to 1000 g/ml) dose-dependently inhibited histamine release from the rat peritoneal mast cells (RPMCs) activated by compound 48/80 or anti-DNP IgE. The level of cAMP in RPMCs, when SSAE was added, increased compared with that of a normal control. In addition, SSAE (0.1 g/ml) had a significant inhibitory effect on anti-DNP IgE-induced $TNF-{\alpha}$ production. Conclusions: These results indicate that SSAE inhibits mast cell-mediated anaphylactic reactions and $TNF-{\alpha}$ production from mast cells.

• The Korea Journal of Herbology
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• v.28 no.5
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• pp.1-6
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• 2013

Objectives : This study was conducted to identify the original Sinomini Caulis et Rhizoma plant among Stephania tetrandra, Cocculus trilobus, and Aristolochiae fangchi to develop the genetic marker for Sinomini Caulis et Rhizoma. Methods : Sinomenium acutum was identified by the classification and identification committee of the National Center for Standardization of Herbal Medicines. The chloroplast ndhF gene was amplified. We performed sequences alignment analysis of Sinomenium acutum, Stephania tetrandra, C. trilobus, and A. fangchi using BioEdit program. The SFR markers designed were consisted of SF01, SR04, and SR05 primers. Results : Many variations of Sinomeni Caulis et Rhizoma are currently commercialized as herbal medicine. We compared the base sequences of the ndhF intergenic space of chloroplast DNA with Sinomenium acutum, Stephania tetrandra, C. trilobus, and A. fangchi. According to the results, it showed that the nucleotide variations were seen in 30 genes of four species. Phylogenetic analysis revealed that 4 species were classified into five groups based on an inter-group divergence in nucleotide sequence of 9%. We developed SFR marker nucleotides enough to authenticate respective species and confirmed its application on the band size at 419 base pair. These sequence differences at corresponding positions were available genetic markers to identity the Sinomeni Caulis et Rhizoma. Conclusions : Base on these results, the ndhF region was effective in distinguishing Sinomini Caulis et Rhizoma The SFR genetic marker was useful for identifying Sinomini Caulis et Rhizoma with other species.

• The Korea Journal of Herbology
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• v.22 no.2
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• pp.109-114
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• 2007

Objectives : A morphological classification among Sinomenium acutum, Cocculus trilobus and Aristolochia fangchi was made through microscopic observation. Method : The slice of the tested material made by paraffin section technique was colored with Safranine Malachite Green contrast methods, and then observed and photographed by olymphus-BHT. Result : 1. Fiber bundles of pericycle in S. acutum are like a crescent moon, stone cells were found among fiber bundles, and starch grain and raphides of calcium oxalate were included in parenchyma. 2. C. trilobus has the fiber bundle of pericycle resembling a crescent moon, but does not have the raphidesof calcium oxalate. 3. A. fangchi has the raphides of calcium oxalate, but does not have the fiber bundles of pericycle. 4. Stems and roots of S. acutum arc mostly similar, but only stems have the pith. Conclusion :' C. trilobus and A. fangchi can be distinguished from each other by the fiber bundles of pericycle and the raphides of calcium oxalate.

• YAKHAK HOEJI
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• v.57 no.3
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• pp.161-166
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• 2013

Sinomenium acutum has been used for the treatment of rheumatoid arthritis, arrhythmia, and pain. We evaluated the quality of S. acutum extract by quantitatively analyzing its components such as sinomenine, magnoflorine and syringaresinol with the simultaneous determination method using HPLC-DAD. A total of 53 samples collected from different localities were evaluated with this quality evaluation method. Sinomenine, magnoflorine and syringaresinol from tested samples ranged from 0.0649~9.1050%, 0.7460~10.7590% and 0.0010~0.2441%, respectively. In the current study, we were able to exhibit the diverse quality of S. acutum samples collected from various locations using the simultaneous determination method.

• Bulletin of the Korean Chemical Society
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• v.33 no.10
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• pp.3455-3457
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• 2012

• Korean Journal of Food Science and Technology
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• v.26 no.5
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• pp.627-632
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• 1994

The growth inhibitions of each strain on tryptic soy broth containing various concentration of 75% ethanol extract of Sinomenium acutum(Thunb.) Rehd. et wils and Glycyrrhiza glabra L. var. glandulifera Regel were compared which were exhibited comparatively strong growth inhibition on Listeria monocytogenes ATCC 19111, 19112, 19113, 19114 and 15313 at previous screening test by disk method. The Sinomenium acutum(Thunb.) Rehd. et wils extract showed partly effect by its concentration in broth to L. monocytogenes ATCC 19111 and 19114. The Glycyrrhiza glabra L. var. glandulifera Regel extract inhibited the growth of all test strains and even 100 ppm of the extract in the broth inhibited the growth of all test strains of L. monocytogenes for 12 to 48 hours. The extract stopped the growth totally of all strains in 500 ppm addition level. L. monocytogenes ATCC 19113 was more sensitive than other strains.

• The Journal of Korean Obstetrics and Gynecology
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• v.20 no.4
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• pp.41-55
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• 2007

Purpose: This study is to examine anti-obesity effect and cytotoxicity of the long-term oral administration of Sinomenium acutum (Bang-gi, SA) Methods: Using diet-induced obesity C57BL/6 mouse model, anti-obesity effect and DNA chip expression and cytotoxicity of the long-term oral administration of this herbal extract were investigated. Results: The herbal extract treated groups were arrested in weight increment only when they were lodged together. Such effects were abolished when they kept individually. SA fed mice behaved very rudely and violently. On the basis of histological studies of liver tissues and also in vitro cytotoxicity tests of the liver and kidney cell lines, no significant toxicity was found by 14 weeks of SA treatments. However, we found significant changes in gene expression profile in SA treated group by micro-array analysis. In case of SA group, up-regulated genes were 1,213 and down-regulated ones were 2,558. Some of lipid metabolism related genes also significantly changed in both treatment groups. Conclusion: SA had effects of increasing the basal metabolic rate by stimulating the sympathetic nervous systems.

• Archives of Pharmacal Research
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• v.29 no.8
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• pp.627-632
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• 2006

Chromatographic separation of the MeOH extract from the stems and rhizomes of Sinomemium acutum led to the isolation of nine alkaloids and a lignan. Their structures were determined to be dauriporphine (1), bianfugecine (2), dauriporphinoline (3), menisporphine (4), (-)-syringaresinol (5), N-feruloyltyramine (6), acutumine (7), dauricumine (8), sinomenine (9), and magnoflorine (10) by spectroscopic means. These compounds were examined for their P-gp mediated MDR reversal activity in human cancer cells. Compound 1 showed the most potent P-gp MDR inhibition activity with an $ED_{50}$ value $0.03\;{\mu}g/mL$ and $0.00010\;{\mu}g/mL$ in the MESSA/DX5 and HCT15 cells, respectively.