• The Microorganisms and Industry
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• v.15 no.2
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• pp.13-19
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• 1989

이제까지 벌견된 SKI 유전자는 SKI2, SKI3, SKI4, SKI6, SKI7, SKI8의 5가지로서 이 중 SKI8만이 cloning된 상태이다. 본연구에서는 SKI3 유전자를 cloning한 후 이 유전자의 염기서열 분석을 통해 SKI3 유전자가 coding하는 단백질의 구조를 밝히고 SKI3 유전자의 killer효모 내에서의 분자생물학적 기능을 밝히고자 하였다.

• Journal of the Korean Institute of Landscape Architecture
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• v.32 no.1
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• pp.92-103
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• 2004

This design was submitted to an invited competition for the Ski Resort of Kangwon Land which was held by Kangwon Land Resort in August, 2003. The site is located at 1∼17 Gohan-ri Gohan-eup Jungsungun Kangwon-do and has an area of 5,000,000$m^2$. The objectives of this project were to revitalize the local economy of Sabuk, Gohan which is well Down for deserted coal mines, by creating a resort complex for family group users with an environmentally friendly ski facility. This design scheme addresses three areas of concerns: First, how to boost the local economy through the proposed ski resort. By limiting lodging facilities in the ski resort, we could induce more development in the nearby towns. In order to balance the economical benefit to towns of Sabuk and Gohan, we decided to have two separate ski base facilities in each town boundary. Second, how to encourage family-oriented use of the ski resort by designing user-friendly ski slope, especially for beginner skiers. We designed 50% more beginner-level ski slope compared to average ski resorts. Third, how to make environmentally-friendly ski resort by respecting the original land form. We minimized the damage to the natural environment by protecting valuable tree groves. Also, ski slopes are designed to reduce unnecessary earth movement. This was designed under the assumption that more valley-oriented slopes would result in less cutting and banking of earthwork； this assumption was verified in comparison with other ski resorts. It is expected that this desist would serve environmentally-friendly designs in ski slopes.

• Korean Journal of Applied Biomechanics
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• v.24 no.3
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• pp.189-199
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• 2014

The purpose of this study was to compare pflugbogen's biomechanical characteristics between on the ski simulator and snowed ski slope to develop interactive ski simulator. Nine ski instructors(sex: male, age: $29.6{\pm}5.4yrs$, height: $176.0{\pm}5.6cm$, body mass: $76.0{\pm}14.0kg$) belong to Korean Ski Instructors Association participated in this research. 24 Infrared cameras for snowed ski slope experiment and 13 infrared camera for ski simulator experiment were installed near by path of pflugbogen. The participants did pflugbogen on the snowed ski slope and the ski simulator both. During the experiment, the participants weared motion capture suit with infrared reflective makers on it, and plantar pressure sensors in ski boots, so that ski motion and plantar pressure data were collected together. Displacement of COG(center of gravity) movements, trunk flexion/extension angle, adduction/abduction angle, and plantar pressure data were significantly different between on the simulator and ski slope. However, percentage of time of COG movement in the phases during medial/lateral and anterior/posterior movement were not significantly different. Findings indicate that the difference between two groups occurred because the ski simulator's drive mechanism is different from ski motion on the slope. In order to develop the ski simulator more interactively for pflugbogen, the ski simulator's drive mechanism need to be reflected 3D motion data of pflugbogen on the slope that were purposed in this research.

• Journal of Fisheries and Marine Sciences Education
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• v.26 no.3
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• pp.553-566
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• 2014

The purpose of this study was to analyze the effect of ski class satisfaction on re-participation of ski focused on double mediations of professor and education images. The results of this study were summarized in the following. Firstly, the satisfaction of ski class was statistically positive effect on re-participation of ski. Second, the indirect effect of the satisfaction of ski on re-participation of ski through the image of professor was statistically positive significant. Third, the indirect effect of the satisfaction of ski on re-participation of ski through the education image was statistically insignificant. Lastly, the indirect effect of the satisfaction of ski on re-participation of ski through the image of professor and education image was statistically insignificant. This result indicated that there was no the relationship of satisfaction of ski and re-participation through the double mediations which were professor and education images.

• Korean Journal of Applied Biomechanics
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• v.30 no.3
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• pp.205-215
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• 2020

Objective: The purpose of this study was to investigate the relative angles and cross-correlation between body segments and ski among four alpine ski turning techniques. Method: 19 alpine ski instructors participated in this study. Each skier asked to perform 4- types of turning technique, classified by radius and level. 8 inertial measurement units were used to measure orientation angle of segment and ski on the anteroposterior and vertical axis. Results: Significant differences were found between types of turning in the segments-ski relative angle on the anteroposterior and vertical axis (p<.05). Although, cross-correlation showed a high correlation between angles of segment and ski, there were significant differences between types of turning. Conclusion: Based on our results, the relative movement and timing between each segment and ski is different according to the turning techniques, so the training methods should be applied differently.

• Korean Journal of Microbiology
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• v.29 no.2
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• pp.75-79
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• 1991

The yeast L-A virus (4.6 kb dsRNA genome) encodes the major coat protein and a "gag-pol" fusion minor coat protein that separately encapsidate itself and $M_{1}$, a 1.8 kb dsRNA satellite virus encoding a secreted protein toxin (the killer toxin). The teast chromosomal SKI genes prevent viral cytopathology by lowering the virus copy number. Thus, $ski^{-}$ mutants are ts and cs for growth. We transformed a ski2-2 virus-infested mutant with a yeast bank in a high copy cloning vector and selected the rare healthy transformants for analysis. One type of transformant segregated M-O L-A-O cells with high frequency. Elimination of the DNA clone from the ski2-2 strain eliminated this phinotype and introduction of the DNA clone recovered from such transformants into the parent ski2-2 strain, or into ski3 or ski6 mutants gave the same phenotype. This killer-curing phenotype was due to the curing of the helper L-A dsRNA virus. The 6.5 kb insert only had this activity when carried on a high copy vector and in $ski^{-}$ cells (not in $SKI^{+}$ cells). This 6.5 kb insert acts as a mutagen on L-A dsRNA producing a high rate of deletion mutations.mutations.

• Journal of Embryo Transfer
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• v.26 no.4
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• pp.229-235
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• 2011

Sloan-Kettering virus gene product of a cellular protooncogene c-Ski is an unique nuclear pro-oncoprotein and belongs to the Ski/Sno proto-oncogene family. Ski plays multiple roles in a variety of cell types, it can induce both oncogenic transformation and terminal muscle differentiation when expressed at high levels. Ski protein is implicated in proliferation/differentiation in a variety of cells. The alternative fate of granulosa cells other than apoptosis is to differentiate to luteal cells, however, it is unknown whether Ski is expressed and has a role in granulosa cells undergoing luteinization. Thus, the aim of this study was, by means of immunohistochemical methods, to locate Ski protein in the rat ovaries during ovulation and corpora lutea (CL) formation to predict the possible involvement of Ski in luteinization. In addition, we performed to examine whether the initiation of luteinization with luteinizing hormone (LH) directly regulates expression of Ski in the luteinized granulosa and luteal cells after ovulation by in vivo models. In order to examine the expression pattern of Ski protein along with the progress of luteinization, follicular growth was induced by administration of equine chorionic gonadtropin to immature female rat, and luteinization was induced by human chorionic gonadtropin treatment to mimic luteinizing hormone (LH) surge. While no Ski-positive granulosa cells were present in preovulatory follicle, Ski protein expression was induced in response to LH surge, and was maintained after the formation of corpus luteum (CL). These results indicate that Ski is profoundly expressed in the luteinized granulosa cells and luteal cells of CL during luteinization, and suggest that Ski may play a role in luteinization of granulosa cells.

• Journal of the Korean Physical Society
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• v.73 no.10
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• pp.1410-1419
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• 2018

A skier's motion was analyzed by a simple model consist of point mass m and a single rod connected to a single ski plate. We studied the conditions for the stable ski turn as functions of the linear velocity and the radius of the turn. The solutions for the stable ski turn in our model do not require any extra skier's movement to complete a stable circular turn. The solution may then give the skier the most comfortable skiing method without any active movement to control the ski. The generalized force supporting the point mass from the ski plate was calculated. We obtained the force from the ground (rebound force) without any geometrical structure of the ski plate. Adding an active movement to the direction of the ski plate, the conditions for the stable ski turn were also analyzed. Our result gives some insight for the skier who wants to develop technique.

• Reproductive and Developmental Biology
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• v.35 no.3
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• pp.355-361
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• 2011

Ski protein is implicated in proliferation/differentiation in a variety of cells. We had previously reported that Ski protein is present in granulosa cells of atretic follicles, but not in preovulatory follicles, suggesting that Ski has a role in apoptosis of granulosa cells. The alternative fate of granulosa cells other than apoptosis is to differentiate to luteal cells, however, it is unknown whether Ski is expressed and has a role in granulosa cells undergoing luteinization. Thus, the aim of the present study was to locate Ski protein in the rat ovary during luteinization to predict the possible role of Ski. In order to examine the expression pattern of Ski protein along with the progress of luteinization, follicular growth was induced by administration of equine chorionic gonadotropin to immature female rat, and luteinization was induced by human chorionic gonadotropin treatment to mimic luteinizing hormone (LH) surge. While no Ski-positive granulosa cells were present in preovulatory follicle, Ski protein expression was induced in response to LH surge, and was maintained after the formation of corpus luteum (CL). Though Ski protein is absent in granulosa cells of preovulatory follicle, its mRNA (c-ski) was expressed and the level was unchanged even after LH surge. Taken together, these results demonstrated that Ski protein expression is induced in granulosa cells upon luteinization, and suggested that its expression is regulated post-transcriptionally.

• The Journal of Natural Sciences
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• v.13 no.1
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• pp.97-111
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• 2003

This study aims at presenting the planning parameter of ski course by designing Gapyung ski course. This site is located at Hacheon-ri, Gapyung-gun, Kyunggi-do and has an area $1,594,001m^2$. Design objectives of Gapyung Ski Resort were to build the most suitable ski course on the site area and make a comparative ski resort. This design process covers the following : site inventory and analysis; integration of design program and terrain analysis; slope planning and trail balance; comparison with other ski resort. This ski resort was composed of 5 lifts and 10 ski trails which are divided into 2 intermediate course, 3 high intermediate course, 4 advanced course, and I expert course. Total length of slope distance is 8,738m. This ski resort will be developed for advanced skiers and snow boarders who enjoy a mogul and a half pipe course. Therefore, the strategy of this ski resort is marked for target market of high intermediate.