• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.108.1-108
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• 2003

Soil metagenome is untapped total microbial genome including that of the majority of unculturable bacteria present in soil. We constructed soil metagenomic library in Escherichia coli using DNA directly extracted from two different soils, pine tree rhizosphere soil and forest topsoil. Metagenomic libraries constructed from pine tree rhizosphere soil and forest topsoil consisted of approximately 33,700 clones and 112,000 clones with average insert DNA size of 35-kb, respectively. Subsequently, we screened the libraries to select clones with antimicrobial activities against Saccharomyces cerevisiae and Agrobacterium tumefaciens using double agar layer method. So far, we have a clone active against S. cerevisiae and a clone active against A. tumefaciens from the forest topsoil library. In vitro mutagenesis and DNA sequence analysis of the antifungal clone revealed the genes involved in the biosynthesis of antimicrobial secondary metabolite. Metagenomic libraries constructed in this study would be subject to search for diverse genetic resources related with useful microbial products.

• Journal of Microbiology and Biotechnology
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• v.14 no.3
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• pp.498-502
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• 2004

The current study was conducted to monitor the possibility of the gene transfer among soil bacteria, including the effect of drift due to rain and surface water, in relation to the release of genetically modified organisms into the environment. Four types of bacteria, each with a distinct antibiotic marker, kanamycin-resistant P. fluorescens, rifampicin-resistant P. putida, chloramphenicol-resistant B. subtilis, and spectinomycin-resistant B. subtilis, were plated using a small-scale soil-core device designed to track drifting microorganisms. After three weeks of culture in the device, no Pseudomonas colonies resistant to both kanamycin and rifampicin were found. Likewise, no Bacillus colonies resistant to both chloramphenicol and spectinomycin were found. The gene transfer from glyphosate-tolerant soybeans to soil bacteria, including Rhizobium spp. as a symbiotic bacteria, was examined by hybridization using the DNA extracted from soil taken from pots, in which glyphosate-tolerant soybeans had been growing for 6 months. The results showed that 35S, T-nos, and EPSPS were observed in the positive control, but not in the DNA extracted from the soilborne microorganisms. In addition, no transgenes, such as the 35S promoter, T-nos, and EPSPS introduced into the GMO soybeans were detected in soilborne bacteria, Rhizobium leguminosarum, thereby strongly rejecting the possibility of gene transfer from the GMO soybeans to the bacterium.

• Structural Engineering and Mechanics
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• v.87 no.2
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• pp.173-189
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• 2023

For a given structural geometry, the stiffness and damping parameters of the soil and the dynamic response of the structure may change in the face of an equivalent linear soil behavior caused by a strong earthquake. Therefore, the influence of equivalent linear soil behavior on the impedance functions form and the seismic response of the soil-structure system has been investigated. Through the substructure method, the seismic response of the selected structure was obtained by an analytical formulation based on the dynamic equilibrium of the soil-structure system modeled by an analog model with three degrees of freedom. Also, the dynamic response of the soil-structure system for a nonlinear soil behavior and for the two types of impedance function forms was also analyzed by 2D finite element modeling using ABAQUS software. The numerical results were compared with those of the analytical solution. After the investigation, the effect of soil nonlinearity clearly showed the critical role of soil stiffness loss under strong shaking, which is more complex than the linear elastic soil behavior, where the energy dissipation depends on the seismic motion amplitude and its frequency, the impedance function types, the shear modulus reduction and the damping increase. Excellent agreement between finite element analysis and analytical results has been obtained due to the reasonable representation of the model.

• Journal of IKEEE
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• v.23 no.1
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• pp.318-321
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• 2019

The apparent soil resistivity is used for estimating multilayer soil parameters, such as, layer's depth and soil resistivity. The apparent soil resistivity can be measured, and also can be calculated if soil parameters are given, becacuse the apparent soil resistivity is a function of these parameters. Therefore, any optimization algorithms can be used to find these parameters which make the calculated apparent soil resistivity close to the measured one. The equation for calculating the apparent soil resistivity is complicated and time consumed, because it is composed of an infinite integral which includes a zero order Bessel's function of the first kind. In this paper, a fast algorithm for calculating the apparent soil resistivity of horizontal multilayer earth structure has been presented using complex image method.

• Journal of Environmental Impact Assessment
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• v.7 no.2
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• pp.37-51
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• 1998

This study was carried out to lead the scientific management of the urban forest by estimating the forest environment. Forest environmental information was constructed using IDRISI system based on survey data, soil, plant, and digital elevation data. Forest environmental information was consisted of soil depth, soil organic content, soil hardness and parent rock as a soil environmental factor, and forest community, tree age, crown density as a plant environmental factor. Plant activity and topographic environment also were analyzed by using remote sensing data and digital elevation data. Environmental function of urban forest was estimated based on results of soil conservation and forest productivity. 70% of urban forest is located in elevation of lower than 200m and 55% of forest area have the slope of lower than 15 degree. Analyzed soil conservation status and forest productivity were almost the same as the soil chemical properties of collected soil sample and the vegetation index estimated using remote sensing data, respectively. Thus, the constructed forest environmental information could be useful to give some ideas for management of urban forest ecosystem and establishment of environmental conservation planning, including forests, in Taejon. The best forest environmental function was appeared at the natural ecology preservation zone. Current natural parks and urban parks were appeared to establish the environmental conservation plan for further development. The worst forest environmental function was appeared at the forest near to the industrial area and an overall and systematic plan was required for the soil management and high forest productivity because these forest was developing a severe soil acidification and having a low forest productivity.

• Journal of The Korean Society of Agricultural Engineers
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• v.55 no.4
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• pp.1-11
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• 2013

Unsaturated hydraulic conductivity function is one of key parameters to solve the flow phenomena in problems of landslide. Prediction models for hydraulic conductivity function related to soil-water retention curve equations in many geotechnical applications have been still used instead of direct measurement of the hydraulic conductivity function since prediction models from soil-water retention curve equations are attractive for their fast and easy use and low cost. However, many researchers found that prediction models for the hydraulic conductivity function can not predict the hydraulic conductivity exactly in comparison with experimental outputs. This research introduced an inverse analysis to evaluate the hydraulic conductivity function corresponding to experimental output from the flow pump system. Optimisation process was carried out to obtain the hydraulic conductivity function. This research showed that the inverse analysis with flow pump system was suitable to assess the hydraulic conductivity in unsaturated soil, and the prediction models for the hydraulic conductivity were led to the significant discrepancy from actual experimental outputs.

• Journal of the Korean Geotechnical Society
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• v.20 no.3
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• pp.47-51
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• 2004

An unsaturated hydraulic conductivity function and a soil-water characteristic curve are the essential constitutive factors in studying unsaturated soils. In order to obtain the unsaturated hydraulic conductivity function, prediction functions, which are based on the soil-water characteristic curve, have been used because it is difficult to measure the unsaturated hydraulic conductivity function directly. In this study, a parameter estimation method using the flow pump technique is introduced to determine the unsaturated hydraulic conductivity function. This method provides more accurate and independent solution than previous methods for the determination of the unsaturated hydraulic conductivity function which is not subordinate to the soil-water characteristic curve or prediction models.

• International Journal of Advanced Culture Technology
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• v.7 no.4
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• pp.268-273
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• 2019

The apparent soil resistivity is used for estimating multilayer soil parameters, such as, layer's depth and soil resistivity. The soil parameters are estimated by continuously revising those parameters until the error between the measured and calculated apparent soil resistivity reaches to allowable level. The equation for calculating the apparent soil resistivity is complicated and time consumed, because it is composed of an infinite integral which includes a zero order Bessel's function of the first kind. In this paper, a fast algorithm for calculating the apparent soil resistivity of horizontal multilayer earth structure is proposed using exponential sampling method.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2003.06a
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• pp.20-23
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• 2003

• Journal of Microbiology and Biotechnology
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• v.15 no.5
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• pp.1130-1134
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• 2005

To monitor the possibility of horizontal gene transfer between transgenic potato and bacteria in the environment, the gene flow from glufosinate-tolerant potato to bacteria in soils was investigated. The soil samples treated with the leaf tissue of either glufosinate-tolerant or glufosinate-sensitive potato were subjected to PCR and Southern hybridization to determine possible occurrence of glufosinate-resistant soil bacteria and to detect the bar (phosphinothricin acetyltransferase) gene, conferring tolerance to glufosinate. The bar gene was not detected from genomic DNAs extracted at different time intervals from the soil samples, which had been treated with the leaf tissue of either transgenic or non-transgenic potato for 2 to 8 weeks. In addition, the level of glufosinate-resistant bacteria isolated from the soil samples treated with the leaf tissue of transgenic potato was similar to that of the samples treated with non-transgenic potato after 4 months of incubation at $25^{\circ}C$. The bar gene was not detected in the genomic DNAs extracted from colonies growing on the plate containing glufosinate, indicating that the bacteria could acquire the resistant phenotype to glufosinate by another mechanism without the uptake of the bar gene from glufosinate-tolerant potato.