• The Korean Journal of Food And Nutrition
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• v.34 no.2
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• pp.137-145
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• 2021

This study was conducted in order to investigate the antioxidant and antimicrobial activity of Solanum nigrum L. fruit powder after undergoing different extraction solvent processes. The total phenolic content of Solanum nigrum L. fruit powder measured a 14.66 GAE mg/g after undergoing ethanol extraction, and the total flavonoid content measured at 201.23 mg CE/g when undergoing ethanol extraction. The ABTS radical scavenging activity was 160.38~209.53 TEAC umol/g, and the DPPH radical scavenging activity was 53.99~90.76 TEAC umol/g, which indicated a higher level of antioxidant power in the ethanol extract as opposed to in the water extract. The FRAP (ferric reducing antioxidant power) of Solanum nigrum L. fruit powder was 115.58~194.58 TEAC umol/g, and B. subtilis KCTC 2189 showed greater antimicrobial activity in the ethanol extract (concentration 200 ug/uL) as opposed to the water extract. Solanum nigrum L. fruit powder revealed differences in antioxidant and antimicrobial activity between the different extraction solvents. In particular, ethanol extract had higher antioxidant and antibacterial activity, meaning it is more favorable for usage as a functional food material.

• Korean Journal of Pharmacognosy
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• v.22 no.2
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• pp.142-143
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• 1991

A steroidal sapogenin, neochlorogenin, was isolated from Solanum nigrum. This is the first report from this plant.

• Journal of the Korea Convergence Society
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• v.9 no.12
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• pp.69-74
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• 2018

The purpose of this study was to antibacterial effect of solanum nigrum extra in candida albicans. In this study, the antibacterial effects of 70% methanol extract and solanum nigraum were investigated. The content of flavonoids and polyphenol contents in solanum nigrum extraction. The antimicrobial activity was determined by spectrophotometric growth inhibition assay(optical density) and colony forming untis (CFU) for the candida albicans. Total contents were flavonoids and polyphenols of solanum nigrum, the result was $8.23{\pm}0.13mg/g$ and $14.97{\pm}1.58mg/g$, respectively. The CFU results showed lower CFU counts in the experimental groups compared to the control group. And the result of the bacterial viability was decreased in the experimental group. Therefore the extracts of solanum nigrum were found to have antimicrobial activity in candida albicans. From the these results, solanum nigrum extracts have a possibility to be a candidate for natural antibacterial components in dental products.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.23
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• pp.10469-10473
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• 2015

Background: Solanum nigrum L. has been used in traditional Chinese medicine because of its diuretic and antipyretic effects. The present research concerned effects of crude polysaccharides isolated from Solanum nigrum L. on erythrocyte membranes of tumor-bearing $S_{180}$ and $H_{22}$ in mice. Materials and Methods: Fluorescence-labeled red blood cell membranes were used with DPH fluorescence spectrophotometry to examine erythrocyte membrane fluidity, and colorimetry to determine degree of erythrocyte surface membrane blocking. Extent of reaction by tumor-bearing mice with the enzyme erythrocyte membrane bubble shadow detection of red cell membrane variation in the degree of closure before and after administration. Results: Solanum nigrum polysaccharide could significantly improve the $S_{180}$ and $H_{22}$ tumor-bearing mice erythrocyte membrane fluidity, compared with the control group, the difference was significant (p<0.01), SNL can significantly improve the red blood cell membrane and then $S_{180}$ tumor-bearing mice sealing ability, compared with the negative control group, the difference was significant(p<0.05, p<0.01). $H_{22}$ tumor-bearing mice can increase red cell membrane and then sealing ability, the difference was significant (p<0.05). Solanum nigrum polysaccharide degree of fluidity and blocking two transplanted tumors in mice restored the ability to raise the red cell membrane has a significant effect. Conclusions: Solanum nigrum L.-type mice transplanted tumor can affect the red blood cell membrane fluidity and re-closed, through the red cell membrane of red blood cells to enhance the immune function of the possibility of erythrocyte immunity against tumor formation garland provide experimental basis.

• Journal of Life Science
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• v.11 no.6
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• pp.509-516
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• 2001

Enzymes and non-enzymatic antioxidants are involved in defense of oxgen free radical intermediates in all aerobic cells. The non -enzymatic antioxidants and antioxidant enzyme from the extracts of Solanum nigrum L. known to be anticancer medicinal plant were examined in other to utilize the discovery in natural products as cancer chem-opereventive agents. The DPPH(1,1-diphenyl-2-picryl-hydrazyl) free radical scavening activity on plant position of Solanum nigrum L. was the highest in root, with stem, whole plant, seed, leaf and flower, at higher activities respectively. In extraction methods, the DPPH free radical scavenging activity by circulating extraction with 80 % MeOH. The DPPH activity of L6 fraction by LH-20 column chromatography showed about 6.7 times higher than that of ethyl acetate-fraction. These were identified as phenolic compounds such as 2-6-methano-3-benzazocin-11-ol, 2[1H]-phyidinethione and 2-hydroxy -5-methyl-benzaldehyde. Peroxidase(POD) and superoxide dismutase(SOD) activities of stem and root were higher than that of other plant positions and those of plant positions according to growing stage were the highest in 60 days after seeding. The numbers of isozyme pattern of POD and SOD showed 10 hands and 5 bands, respectively, especially, 8 bands of POD and 3 bands of SOC showed a difference according to plant positions.

• Journal of Life Science
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• v.24 no.3
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• pp.234-241
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• 2014

To identify and isolate anticancer active compounds from Solanum nigrum, S. nigrum was extracted with MeOH and then fractionated with various organic solvents ($CH_2Cl_2$, EtOAc, n-BuOH, and $H_2O$). The cytotoxic effects of the MeOH extracts from S. nigrum and its organic solvent-soluble fractions were also tested in HT29 cells. All the MeOH extracts of S. nigrum and its organic-solvent extracts induced cytotoxicity in the HT29 cells. Among the extracts, $H_2O$ was the most effective. The $H_2O$ extract was purified further by repeated silica gel, Sephadex LH-20, Diaion HP- 20, and RP-18 column chromatography. An active anticancer compound, Des-N-26-methylene-dihydrotomatidine, was isolated with a molecular weight of 416 and a molecular formula of $C_{28}H_{48}O_2$. Analysis of the cytotoxic effects of Des-N-26-methylene-dihydrotomatidine on the HT29 cells compared to those of tomatine and tomatidine are similar in its structure, is higher than tomatidine above the 40 ${\mu}g/ml$ concentration, but lower than tomatine. This is the first study to describe the anticancer activity of Des-N-26-methylene-dihydrotomatidin, isolated from S. nigrum. Des-N-26- methylene-dihydrotomatidine seems to have potential as a natural bioactive compound.

• Korean Journal of Medicinal Crop Science
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• v.2 no.1
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• pp.38-43
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• 1994

The effect of thidiazuron on callus growth and shoot regeneration of Solanumspecies was very positive. Increasing concentrations of thidiazuron stimulated callus growth of Solanum ptycanthum and Solanum nigrum. Shoot regeneration of S. ptycanthum and S. nigrum was greater on medium with thidiazuron than that with IAA and BA. Thidiazuron at $0.5{\mu}M$ or above increased the number of shoots regenerated from S. ptycanthum calli compared to the IAA with BA. High concentrations of thidiazuron $( >I{\mu}M)$ increased the number of shoots than BA or low levels of thidiazuron and IAA or BA The addition of IAA to thidiazuron media reduced S. ptycanthum shoot formation.

• Journal of the Korean Society of Costume
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• v.57 no.4 s.113
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• pp.61-69
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• 2007

The purpose of this study was to identify the utility value with material of natural dyeing and to examine the dyeability and antibiosis of fabrics dyed with Solanum nigrum extract. The results obtained are as follows. First, the color tone of dyed fabrics was tinged with from yellow series to green series, in the chrominance the fabric dyed with Fe mordant was showed the highest value. Second, colorfastness to dyeing showed difference as to mordants, but colorfastness to dyeing except for sunlight colorfastness showed the good colorfastness result on the whole. Third, in the test results of antibiosis, the dyed both cotton and silk fabrics showed the very excellent antibiosis effect for bacteria but showed the insignificant effect for fungus.

• Journal of Plant Biology
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• v.14 no.2
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• pp.7-10
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• 1971

Haploid cell obta-ined from microspores of Solanum nigrum were cultured on two kinds of medium, "Callus-inducing medium" and "Differentiation medium", in order to conduct histological studies of callus and examine differentiation of plantlets. On the callus-inducing medium the calli grew rapidly. The bulk of callus mass was light brown colored "Wet callus" covered on the surface with thin layers of rough and gleaming "White callus". The wet callus was consisted of parenchyma and meristematic tissues, while the white callus had no meristematic tissues. Large parenchyma cells, by successive divisions, became multicellular or poly nucleate cells which developed later to be meristematic tissues. The calli embedded on the differentiation medium quickly turned to dark brown color. Plantlets, however, came out later from these blackened callus mass. In the callus sectioned about ten weeks after imbedding on the differentiation medium, radially elongated tissue, concentric tissue, epidermis, tracheid-like structure, and plant jprimordia were observed.ure, and plant jprimordia were observed.

• Korean Journal of Plant Resources
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• v.25 no.5
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• pp.596-602
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• 2012

This study was carried out to effect on Growth Characteristics and Yield of Solanum njgrum L. The experimental results are summarized as follows. Germination, growth characteristics and yield of Solanum nigrum Linne collected from southern parts of Korea were investigated. The one fruit weight was the heaviest in Gochang traditional S. nigrum among the collected sites. The number of seeds in one fruit was high in the orders of Gochang traditional sites, Gwangju traditional sites, Suncheon traditional sites and Naju traditional sites. The earliest flowering of Solanum nigrum was observed on 10. August. The plant height, stem diameter, leaf numbers and branch numbers were ranged from 65 cm to 75 cm, 7.1 mm to 8.2 mm, 237 ea to 270 ea and 12 ea to 17 ea, respectively. The fresh weight per plants were ranged from 265 g to 279 g, and yield of biomass were ranged from 1,298 kg to 1,367 kg per 10a. The insect pest numbers of S. nigrum according to the collected sites were showed no significant in this test.