• Title/Summary/Keyword: Spectrophotometer

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Quantitative Measurement of Spray Deposit Using Spectrophotometer (Spectrophotometer를 이용한 농약의 부착량 측정방법)

  • 이중용;안성용;정창주
    • Journal of Biosystems Engineering
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    • v.24 no.6
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    • pp.479-486
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    • 1999
  • A measurement method for the deposition amount of spray, using tracer and spectrophotometer was developed. Food colors was selected as tracer, because it was cheep and easily treatable. Using NIRS(Near Infrared Reflection Spectrophotometer), regression curves between absorbance spectrum and concentration of the tracer were obtained. Yellow food colors showed the peak of spectrum at 452nm, and absorbance of peak showed a tendency to increase as concentration increased. Also, the possibility of concentration control by heating was investigated.

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The Proposed Validation Guidelines and Their Application for Performance Verification of UV-VIS Spectrophotometer (UV-VIS 분광기기의 성능검증을 위한 밸리데이션 가이드라인의 제정 및 응용)

  • Jin, Jing-Yu;Kang, Young-Koo;Lee, Won-Jae
    • YAKHAK HOEJI
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    • v.50 no.3
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    • pp.154-159
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    • 2006
  • For the increasing demand for the validation of analytical results, one of the important concerns of the analyst is to be assured that the instrumentation is working to qualified conditions. In this study, we have investigated and compared several validation guidelines of the performance verification of UV-VIS spectrophotometer used in pharmacopoeias of major countries and UV-VIS instrument analytical companies, and we proposed the practically useful validation guidelines of UV-VIS spectrophotometer. Based on our proposed validation guidelines of UV-VIS spectrophotometer, several UV-VIS spectrophotometers used in drug manufacturing laboratories were validated for their performances.

Study of Analytical Method for Diesel Fuel Using UV-Spectrophotometer in Water Samples (UV-Spectrophotometer 를 이용한 수중 경유 분석법)

  • Lee, Jong-Sik;Jung, Kwang-Yong
    • Korean Journal of Environmental Agriculture
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    • v.18 no.1
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    • pp.83-85
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    • 1999
  • This study was conducted to find out analytical method for diesel fuel using uv-spectrophotometer in water samples. The optimum wavelength, detection limit and recoveries for desel fuel extracted with n-pentane were 257nm, $100{\sim}800mg/l$ and $102{\sim}121%$, respectively. This method were stable for measuring concentrations of desel fuel for 1 hour at $20{\pm}1^{\circ}C$ condition.

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Evaluation of illumination effect for on-board spectrometer system (내장형 분광광도시스템 구성에 따른 조명 영향 평가)

  • Lee, Sangsik;Lee, Choongho
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.3 no.2
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    • pp.18-24
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    • 2010
  • On-board spectrophotometer has been widely used as a non-contact and non-destructive measurement system in many fields. In this study, we evaluated the effect with respect to the light of on-board spectrophotometer based on a comparison with the standard spectrophotometer and color information coordinate system. Red, yellow, green and blue color paper, which were the standard reflective color paper and Munsell color paper, were used for experiments. In order to compare between a standard spectrophotometer system and an on-board spectrophotometer system, each color paper was measured 20 times. We concluded that it is possible to develop a system regardless the effect of light if the light was supplied consistently and a calibration was performed exactly while we applied an on-board spectrophotometer to non-contact and non-destructive measurement system.

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A Study on the Charateristics of ORMOSIL as a Dye Host by Measurement of UV/VIS Spectrophotometer (UV-VIS Spectrophotometer를 이용한 ORMOSIL의 투광특성)

  • Kim, Goo-Dae;Lee, Dong-A;Kang, Hyun-Gyu;Moon, Ji-Woong
    • Analytical Science and Technology
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    • v.7 no.1
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    • pp.17-23
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    • 1994
  • Through sol-gel process, inorganic/organic composite as a dye host was made. It was observed that this composite has about 90% of trnasmittance by UV/VIS spectrophotometer. The transmittance is the most important requirement to decide whether the composite could apply or not as the dye host. The transmittance was increased with decreasing HCl as a catalyst, aging time, and amount of PDMS. Also, fluorescent and transmittant charateristics of Rhodamine 6G Perchlorate that was doped into ORMOSIL were measured. The fluorescent intensity was increased and the trnasmittance was decreased with rising gelling temperature.

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Evaluation of illuminant effect of NIR(Near Infrared Radiation) using spectrophotometer for medicine (의학용 분광광도계를 이용한 근적외선 대역의 조명 영향평가)

  • Lee, Sangsik
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.3 no.4
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    • pp.16-22
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    • 2010
  • In this paper, we evaluated the effect with respect to the light of 700-1,100 nm NIR(near infrared radiation) for spectrophotometer. Standard, red, yellow, green and blue color paper which was Munsell color paper were used for experiments. Our used light is incandescent lamp, fluorescent lamp, invert fluorescent lamp and combined lamp. Each color paper was measured 20 times. Therefore we concluded that it is possible to build a spectrophotometer for NIR(near infrared radiation) measurement we applied an spectrophotometer to measurement system in incandescent lamp.

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Identification of Lanosterol and Ergosterol in Sarcodon aspratus Berk. (S. Ito) (능이버섯에서의 lanosterol과 ergosterol의 확인)

  • 김종봉;박홍덕
    • Journal of Life Science
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    • v.10 no.6
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    • pp.617-620
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    • 2000
  • Methanol extract of Sarcodon aspratus Berk. (S. Ito) was analyzed by thi layer chromatography, gas chromatography and mass spectrophotometer. Nine fractions from primary methanol extract were observed by TLC. Six fractions were observed by the second TLC analysis of the second and the third fractions. 27,28-digydrolanosterol({TEX}$C_{30}H_{52}${/TEX}O), ergost-7-en-3-ol({TEX}$C_{28}H_{48}${/TEX}O) were identified from two fractions of the second TLC analysis by mass spectrophotometer. The molecular weights of 27,28-dihydrolanosterol and ergost-7-en-3-ol were 413 and 400 respectively.

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Measurement of Spray Deposit Amount Using Spectrophotometer and Food Dye as Tracer

  • Rhee, J.Y.;Ahn, S.Y.
    • Agricultural and Biosystems Engineering
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    • v.1 no.1
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    • pp.16-21
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    • 2000
  • Measurement of spray deposit is necessary for evaluation of a chemical application technology. However it is not easy and time consuming. A simple method for measuring the deposition amount of spray using a tracer and a spectrophotometer was developed. Various materials were tested to determine an adequate tracer. Food dye was selected as a tracer, because it was cheep and easily treatable. Using NIRS(Near Infrared Reflection Spectrophotometer), a regression curves between maximum absorbance of a solution and concentration of the tracer were obtained. Yellow food dye solution showed a peak of spectrum at 452 nm, and absorbance of peak showed a tendency to increase as concentration increased. Green or pink food dye were tested and judged to be good tracers. However, tracer concentration should not exceed certain limits in order to measure maximum absorption. Using spraying liquid with known tracer concentration and known amount of washing liquid, spray deposit amount on real targets on leaves could be estimated at less than 13% error level.

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The Analysis of the UV cut-off effect of tinted lens and the Characteristic of UV tester (착색렌즈의 UV 차단효과와 UV TESTER 평가 분석)

  • Park, Moon Chan;Jung, Boo Young
    • Journal of Korean Ophthalmic Optics Society
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    • v.7 no.2
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    • pp.129-134
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    • 2002
  • We obtained the UV cut-off effect of tinted CR-39 Ophthalmic lens with the respect of concentration of green, brown, blue color. First, we analyzed the UV spectrum of tinted CR-39 lens measured by Spectrophotometer and then compared the UV data by Spectrophotometer with UV data by UV tester used in an optician shop. The result obtained show that the UV cut-off effect is increased with color concentration of tinted CR-39 lens, and the transmittance of 0%~5% at 400nm by Spectrophotometer is correspondence with transmittance of 0% by UV tester.

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