• BMB Reports
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• 제40권5호
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• pp.708-714
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• 2007

The two-component signal transduction, which typically consists of a histidine kinase and a response regulator, is used by bacterial cells to sense changes in their environment. Previously, the SphS-SphR histidine kinase and response regulator pair of phosphate sensing signal transduction has been identified in Synechocystis sp. PCC 6803. In addition, some response regulators in bacteria have been shown to be cross regulated by low molecular weight phosphorylated compounds in the absence of the cognate histidine kinase. The ability of an endogenous acetyl phosphate to phosphorylate the response regulator, SphR in the absence of the cognate histidine kinase, SphS was therefore tested in Synechocystis sp. PCC 6803. The mutant lacking functional SphS and acetate kinase showed no detectable alkaline phosphatase activity under phosphate-limiting growth conditions. The results suggested that the endogenous acetyl phosphate accumulated inside the mutants could not activate the SphR via phosphorylation. On the other hand, exogenous acetyl phosphate could allow the mutant lacking functional acetate kinase and phosphotransacetylase to grow under phosphate-limiting conditions suggesting the role of acetyl phosphate as an energy source. Reverse transcription PCR demonstrated that the transcripts of acetate kinase and phospho-transacetylase genes in Synechocystis sp. PCC 6803 is up-regulated in response to phosphate limitation suggesting the importance of these two enzymes for energy metabolism in Synechocystis cells

• International Journal of Naval Architecture and Ocean Engineering
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• 제9권4호
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• pp.390-403
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• 2017

Smoothed Particle Hydrodynamics (SPH) method has a good adaptability for the simulation of free surface flow problems. There are two forms of SPH. One is weak compressible SPH and the other one is incompressible SPH (ISPH). Compared with the former one, ISPH method performs better in many cases. ISPH based on Rankine source solution can perform better than traditional ISPH, as it can use larger stepping length by avoiding the second order derivative in pressure Poisson equation. However, ISPH_R method needs to solve the sparse linear matrix for pressure Poisson equation, which is one of the most expensive parts during one time stepping calculation. Iterative methods are normally used for solving Poisson equation with large particle numbers. However, there are many iterative methods available and the question for using which one is still open. In this paper, three iterative methods, CGS, Bi-CGstab and GMRES are compared, which are suitable and typical for large unsymmetrical sparse matrix solutions. According to the numerical tests on different cases, still water test, dam breaking, violent tank sloshing, solitary wave slamming, the GMRES method is more efficient than CGS and Bi-CGstab for ISPH method.

• 한국터널지하공간학회 논문집
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• 제15권3호
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• pp.345-356
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• 2013

본 연구에서는 SPH(Smoothed Particle Hydrodynamics) 코드를 사용하여 TBM에 장착되는 디스크커터에 의한 암석의 절삭과정을 모사하였다. 이를 위하여 본 연구에서는 3차원 FEM 해석 상용프로그램인 AUTODYN3D를 사용하였으며 이를 통해 국내 황등화강암을 대상으로 총 25개의 절삭조건에 대한 수치적인 절삭시험을 수행하였다. 암석과 디스크커터를 각각 라그란지안 솔버와 SPH 솔버를 사용하여 3차원 형상으로 모델링 하고 두 개의 디스크커터가 순차적으로 암석을 절삭하도록 모델링 하였다. 수치해석과 LCM시험에서 측정한 디스크커터의 작용력은 오차 10%이내의 값을 보여 대체로 일치하는 것으로 나타났고 균열의 전파양상과 암석의 파쇄양상 또한 유사한 것으로 나타났다. 또한 절삭된 최적 커터간격을 측정한 결과 LCM시험 결과와 일치하였다. 이를 통해 SPH코드를 사용한 수치해석기법의 적용성을 확인할 수 있었으나 해석시간을 단축하기 위한 Lagrange-SPH코드의 커플링에 관한 후속연구가 필요할 것으로 판단되었다.

• Asian Pacific Journal of Cancer Prevention
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• 제13권2호
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• pp.625-631
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• 2012

In order to investigate whether SKI-II could reverse drug resistance and its possible mechanisms, we treated SGC7901/DDP cells with SKI-II or SKI-II in combination with DDP. Then cell growth, apoptosis, micromorphological changes, and expression of SphK1, P-gp, NF-${\kappa}B$, Bcl-2 and Bax were assessed by MTT assay, flow cytometry, electron microscopy, immunocytochemistry and Western blot assay respectively. SGC7901/DDP cells were insensitive to cisplatin 2.5mg/L, but when pretreated with SKI-II, their proliferation was inhibited by cisplatin 2.5mg/L significantly, the inhibition rate increasing with time and dose. The apoptosis rate was also significantly elevated. Expression of SphK1 and P-gp was decreased significantly, Pearson correlation analysis showing significant correlation between the two (r=0.595, P<0.01). Expression of NF-${\kappa}B$ and Bcl-2 was decreased significantly,while that of Bax was increased, compared to the control group. There were significant correlations between SphK1 and NF-${\kappa}B$(r=0.723, P<0.01), NF-${\kappa}B$ and Bcl-2(r=0.768, P<0.01). All these data indicated that SKI-II could reverse drug resistance of SGC7901/DDP to cisplatin by down-regulating expression of P-gp and up-regulating apoptosis through down-regulation of SphK1. The increased apoptotic sensitivity of SGC7901/DDP to cisplatin was due to the decreasing proportion of Bcl-2/Bax via down-regulating NF-${\kappa}B$.

• 천문학회보
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• 제43권1호
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• pp.32.2-32.2
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• 2018

We present the structural parameters of ~ 910 dwarf elliptical-like galaxies in the local universe ($z{\lesssim}0.01$) derived from the r-band images of the Sloan Digital SKy Survey (SDSS). We examine the dependence of structural parameters on the morphological types (dS0, dE, dEbc, dSph, and dEblue) and the environment. There is not much difference in the structural parameters among the five subtypes but the mean surface brightness within the effective radius (<${\mu}e$>) of dSph galaxies is clearly different from that of other subtypes. The frequency of disk features such as spiral arm, bar, lens, and rings strongly depends on the morphology of dwarf elliptical-like galaxies with no disk features in dSph galaxies. The absence of disk features and the low surface brightness of dSph galaxies are thought to be closely related to their low mass which leads to different evolution from other subtypes of dwarf elliptical-like galaxies. Density Environments Using IMSNG.

• Annals of Surgical Treatment and Research
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• 제95권5호
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• pp.233-239
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• 2018

Purpose: In Korea, the working-hour limitation regulation has been implemented in December 2017. We aimed to define the difference in operative experience of surgical residents before and after implementing this policy in 2 hospitals among 8 affiliated hospitals of the Catholic Medical Center where implemented the 80 working-hour limitation policy since March 2015. Methods: All the operation records were reviewed, and the number of resident-participated surgeries between March and August in 2002 and 2017 were compared. Operations performed or participated in by residents as first assistants were defined as resident participated surgery. Results: After 2 years from the initiation of the resident work-hour limitations, the number of resident participated surgery has slightly decreased in both hospitals (Yeouido St. Mary's Hospital [YSM]: 317 to 302, St. Paul Hospital [SPH]: 635 to 461). For each resident, changes were like followings: $0{\rightarrow}21$ cases for R1, $65{\rightarrow}72$ cases for R2, $83{\rightarrow}192$ cases for R3, and $169{\rightarrow}17$ cases for R4 in YSM. In SPH, number of resident participating surgery was changed like followings: $4{\rightarrow}32$ cases for R1, $222{\rightarrow}100$ cases for R2, $317{\rightarrow}300$ cases for R3, and $92{\rightarrow}29$ cases for R4. In both hospital, while, total number of resident participating oncologic surgery has been decreased, number of resident participating appendectomy has been far increased. Activity of each grade resident is different according to hospital. Conclusion: Although total number of resident participating surgery decreased, variable changes were observed in each grade of resident according to each type of surgery and different hospitals. It is believed that comparisons of experiences from more hospitals in the future would be helpful in establishing the guidelines for surgical experience requirement of residents in Korea.

• 천문학회지
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• 제29권1호
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• pp.19-30
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• 1996

We have examined consequences of strong tidal encounters between a neutron star and a normal star using SPH as a possible formation mechanism of isolated recycled pulsars in globular clusters. We have made a number of SPH simulations for close encounters between a main-sequence star of mass ranging from 0.2 to 0.7 $M_\bigodot$ represented by an n=3/2 poly trope and a neutron star represented by a point mass. The outcomes of the first encounters are found to be dependent only on the dimensionless parameter $\eta'{\equiv}(m/(m+ M))^{1/2}(\gamma_{min}/R_{MS})^{3/2}(m/M)^{{1/6)}$, where m and M are the mass of the main-sequence star and the neutron star, respectively, $\gamma_{min}$ the minimum separation between two stars, and $R_{MS}$ the size of the main-sequence star. The material from the (at least partially) disrupted star forms a disk around the neutron star. If all material in the disk is to be acctreted onto the neutron star's surface, the mass of the disk is enough to spin up the neutron star to spin period of 1 ms.

• 천문학회지
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• 제50권4호
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• pp.111-124
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• 2017

The environmental dependence of the morphology of dwarf galaxies in isolated satellite systems is analyzed to understand the origin of the dwarf galaxy morphology using the visually classified morphological types of 5836 local galaxies with $z{\leq}0.01$. We consider six sub-types of dwarf galaxies, dS0, dE, $dE_{bc}$, dSph, $dE_{blue}$, and dI, of which the first four sub-types are considered as early-type and the last two as late-type. The environmental parameters we consider are the projected distance from the host galaxy ($r_p$), local and global background densities, and the host morphology. The spatial distributions of dwarf satellites of early-type galaxies are much different from those of dwarf satellites of late-type galaxies, suggesting the host morphology combined with $r_p$ plays a decisive role on the morphology of the dwarf satellite galaxies. The local and global background densities play no significant role on the morphology of dwarfs in the satellite systems hosted by early-type galaxies. However, in the satellite system hosted by late-type galaxies, the global background densities of dE and dSph satellites are significantly different from those of $dE_{bc}$, $dE_{blue}$, and dI satellites. The blue-cored dwarf satellites ($dE_{bc}$) of early-type galaxies are likely to be located at $r_p$ > 0.3 Mpc to keep their cold gas from the ram pressure stripping by the hot corona of early-type galaxies. The spatial distribution of $dE_{bc}$ satellites of early-type galaxies and their global background densities suggest that their cold gas is intergalactic material accreted before they fall into the satellite systems.

• 한국군사과학기술학회지
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• 제20권6호
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• pp.743-751
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• 2017

To evaluate the structural integrity of the helicopter radome, we performed bird strike analysis using SPH (Smoothed Particle Hydrodynamics) technique. Since the SPH method is a meshfree method, there is no phenomenon such as mesh tangling and it is suitable to predict the dispersion behavior of debris and debris cloud generated by high-speed impact. In order to observe the scattering direction of fractured bolts, the analysis were performed under the condition that the fracture occurs at the proof load. As a result of bird strike analysis, there is no secondary damage as well as the damage due to, the dispersion behavior of the bird model, and the scattering of the fractured bolts and radome. From the additional analysis that were performed to determine the actual bolt fracture, only plastic deformation is predicted since the maximum stress of the bolt does not exceed the ultimate stress.

• 한국전산구조공학회논문집
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• 제26권5호
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• pp.343-349
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• 2013

항공기 연료셀은 추락 상황에서 승무원의 생존성과 직결되는 중요 구성품으로 회전익 항공기에 적용되고 있는 내충격성 연료셀은 추락시 승무원의 생존성 향상에 큰 역할을 하고 있다. 미육군은 항공기가 처할수 있는 다양한 상황에서 연료셀이 제 기능을 발휘할 수 있도록 1960년대 초부터 MIL-DTL-27422 이라는 연료셀 개발규격을 제정하여 현재까지 적용해 오고 있다. 해당 개발규격에 규정된 시험 중에서 충돌충격시험은 연료셀의 내충격 성능을 검증하는 시험으로써, 해당 시험을 통과하는 연료셀은 생존가능 충돌환경에서 화재가 발생하지 않아 승무원의 생존성이 대폭 향상될 수 있음을 의미한다. 그러나 충돌충격시험은 작용하는 하중 수준이 너무 높기 때문에 실패 위험성이 가장 큰 시험이기도 하다. 연료셀이 해당 시험을 통과하지 못하는 경우에는 재시험을 위한 비용과 준비기간이 상당히 소요되어 항공기 개발일정에 심각한 지장을 초래할 가능성도 높다. 따라서, 연료셀 설계 초기부터 내충격성능 만족여부에 대한 예측을 위해 충돌충격시험의 수치해석을 통한 실물시험에서의 실패 가능성을 최소화해야 한다는 필요성이 제기되어 왔다. 본 연구에서는 충돌모사 프로그램인 LS-DYNA에서 지원하는 유체-구조 연성해석 방법인 SPH 방법을 사용하여 연료셀 충돌충격시험 수치 모사를 수행하였다. 수치해석 조건으로 MIL-DTL-27422에서 요구하는 시험조건을 고려하였고, 실물 연료셀의 시편시험을 통해 확보한 물성데이타를 해석에 반영하였다. 그 결과로 연료셀 자체의 응력수준을 평가하고 취약부위에 대한 고찰을 수행하였다.